Journal of Physiology and Pathophysiology
Subscribe to JPAP
Full Name*
Email Address*

Article Number - 3336A20566

Vol.3(2), pp. 12-19 , March 2012

ISSN: 2141-260X

 Total Views: 0
 Downloaded: 0

Full Length Research Paper

The study of 24 h post treatment effects of the aqueous extract of Rosmarinus officinalis after renal ischemia/reperfusion in rat

Marzieh Zohrabi1, Saeed Changizi Ashtiyani1*, Saeed Hajihashemi1, Akbar Hassanpoor2 and Nasser Hosseini3


1Department of Physiology, Arak University of Medical Sciences, Arak, Iran.

2Department of Pathology, Arak University of Medical Sciences, Arak, Iran.

3Department of Medicinal Plants, Arak University, Arak, Iran.

Email: [email protected]

 Accepted: 10 February 2012  Published: 30 March 2012

Copyright © 2012 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Ischemia/reperfusion (I/R) induced acute renal failure (ARF), causes renal functional and histological disorders. This study investigated the effect of oral Rosemary extract on ARF disorders in rat. Forty male Sprague-Dawley rats were used in 4 groups (n=10) as control, sham, I/R with gavage feeding normal saline, and I/R with gavage feeding of 8% aqueous extract of Rosemary (10 ml/kg/day). ARF was induced by bilateral renal artery and vein occlusion for 30 min. Reperfusion period was continued for 24 h. Blood pressure and renal blood flow of rats were measured using a power lab system (AD instruments). After placing the rats into metabolic cages, urine sampling was done. Plasma samples were obtained and after removing both kidneys and staining kidney sections, histological studies were performed. Data were analyzed using SPSS software version 11.5. The comparison between Rosemary and vehicle groups indicated significant reduction in [Cr]p (P< 0.001), [BUN]P (P < 0. 01), UNaV° (P < 0.001), and significant increase in UKV° (P< 0.001) in the Rosemary group. Oxidative stress studies indicated the ability of the aqueous extract of Rosemary to decrease malondialdehyde (MDA) and increase ferric reducing antioxidant power (FRAP) in comparison with the I/R group. Histopathological analyses indicated significant decreases in vessels congestion, tubules disorder, and space of Bowman's capsule in the Rosemary group in comparison with the vehicle groups. Post treatment with Rosemary aqueous extract protects the rats against histological injury and functional impairment induced by 24 h I/R.


Key words: Acute renal failure, ischemia/reperfusion, Kidney, rat, Rosmarinus officinalis.

Abuelo JG (2007). Normotensive ischemic acute renal failure. N. Engl. J. Med., 357: 797-805.
Ashtiyani SC, Moosavi SMS, Hosseinkhani S, Shirazi M (2008). The effects of vitamin-E on oxidative stress and metabolic imbalance induced by acute unilateral ureteral obstruction in anaesthetized rats. Tehran University Med. J., 66: 617-624.
Bagshaw SM, Bellomo R (2007). Acute renal failure. Surgery, 25: 391-398.
Barnes J, Anderson LA, Phillipson JD (2007). Textbook of Herbal Medicines, 3rd edn. Pharmaceutical Press, London, Chicago.
Basile DP, Friedrich JL, Spahic J, Knipe N, Mang H, Leonard EC, Changizi-Ashtiyani S, Bacallao RL, Molitoris BA, Sutton TA (2011). Impaired endothelial proliferation and mesenchymal transition contribute to vascular rarefaction following acute kidney injury. Am. J. Physiol. Renal. Physiol., 300: 721-33.
PMid:21123492 PMCid:PMC3064142
Bayrak O, Bavbek N, Karatas OF, Bayrak R, Catal F,Cimentepe E, Akbas A, Yildirim E, Unal D, Akcay A (2008). Nigella sativa protects against ischaemia/reperfusion injury in rat kidneys. Nephrol. Dial. Transplant, 23: 2206-2212.
Chatterjee PK, Cuzzocrea S, Brown PA, Zacharowski K, Stewart KN, Mota-Filipe H, Thiemermann C (2000a). Tempol, a membrane-permeable radical scavenger, reduces oxidant stress-mediated renal dysfunction and injury in the rat. Kidney. Int. 58: 658-673.
Chatterjee PK, Zacharowski K, Cuzzocrea S, Otto M, Thiemermann C (2000b). Inhibitors of poly (ADP-ribose) synthetase reduce renal ischemia-reperfusion injury in the anesthetized rat in vivo. FASEB J., 14: 641-51.
Conger J (1997). Hemodinamic factors in acute renal failure. Adv. Ren. Replace. Ther., 4: 25-37.
Doolaege EHA, Raes DK, Vos FD, Verhe R, Smet SD (2011). Absorption, distribution and elimination of carnosic acid, a natural antioxidant from Rosmarinus officinalis in rats. Plant. Foods. Hum. Nutr., 66: 196-202.
Frishman WH, Sinatra ST, Moizuddin M (2004). The use of herbs for treating cardiovascular disease. Semin. Integr. Med., 2: 23-35.
Gimelreich D, Popovtzer MM, Wald H, Pizov G, Berlatzky Y, Rubinger D (2001). Regulation of ROMK and channel-inducing factor (CHIF) in acute renal failure due to ischemic reperfusion injury. Kidney Int., 59: 1812-1820.
Gong H, Wang W, Kwon TH, Jonassen T, Li C, Ring T, Frokler J, Nielsen S (2004). EPO and a-MSH prevent ischemia/reperfusion-induced down-regulation of AQPs and sodium transporters in rat kidney. Kidney Int., 66: 683-695.
Haloui M, Louedec L, Michel JB, Lyoussi B (2000). Experimental diuretic effects of Rosmarinus officinalis and Centaurium erythraea. J. Ethnopharmacol., 71: 465-472.
Han KH, Kim HY, Croker BP, Reungjui S, Lee SY, Kim J, Handlogten ME, Adin CA, Weiner ID (2007). Effects of ischemia- eperfusion injury on renal ammonia metabolism and the collecting duct. Am. J. Physiol. Renal. Physiol., 293: 1342-1354.
Haraguchi H, Saito T, Okamura N, Yagi A (1995). Inhibition of lipid peroxidation and superoxide generation by diterpenoids from Rosmarinus officinalis. Planta Med., 61: 333-336.
Hosseinzadeh H, Nourbakhsh M (1989). Negative inotropic action of Rosemary oil, 1,8-cineole and bornyl acetate. Planta Med., 55: 106-107.
Juhas S, Bukovska A, Cikos S, Czikkova S, Fabian D, Koppel J (2009). Anti-inflammatory effects of Rosmarinus officinalis essential oil in mice. Acta Vet. Brno., 78: 121-127.
Kenji O (2004). Prevention of endothelial cell injury by activated protein C: the molecular mechanism (s) and therapeutic implications. Curr. Vasc. Pharmacol., 2: 125-133.
Kramer AA, Postler G, Salhab KF, Mendez C, Carey LC, Rabb H (1999). Renal ischemia/reperfusion leads to macrophage-mediated increase in pulmonary vascular permeability. Kidney Int., 55: 2362-2367.
Mashiach E, Sela S, Weinstein T, Cohen HI, Shasha SM, Kristal B (2001). Mesna: A novel renoprotective antioxidant in ischaemic acute renal failure. Nephrol. Dial. Transplant, 16: 542-551.
Moosavi SMS, Ashtiyani SC, Hosseinkhani S (2011). L-Carnitine Improves Oxidative Stress and Suppressed Energy Metabolism but Not Renal Dysfunction Following Release of Acute Unilateral Ureteral Obstruction in Rat. Neurourol. Urodyn., 30: 480-487.
Moosavi SMS, Ashtiyani SC, Hosseinkhani S (2010). Comparison of the effects of L-Carnitine and alpha-tocopherol on acute ureteral obstruction-induced renal oxidative imbalance and altered energy metabolism in rats. J. Urol. Res., 38: 187-194.
Moosavi SMS, Bayat G, Owji SM, Panjehshahin MR (2009). Early renal post-ischaemic tissue damage and dysfunction with contribution of A1-adenosine receptor activation in rat. Nephrology, 14: 179-188.
Moosavi SMS, Barmaki B, Geramizadeh B, Fallahzadeh MH, Johns EJ (2004). Effect of Endothelin-A Receptor Blockade on the Early Phase of Ischemia/Reperfusion- Induced Acute Renal Failure in Anesthetized Rats. Iranian J. Med. Sci., 29: 14-20.
Naemura A, Ura M, Yamashita T, Arai R, Yamamoto J (2008). Long-term intake of Rosemary and common thyme herbs inhibits experimental thrombosis without prolongation of bleeding time. Thromb. Res., 122: 517-522.
Pechman KR, De Miguel C, Lund H, Leonard EC, Basile DP, Mattson DL (2009). Recovery from renal ischemia-reperfusion injury is associated with altered renal hemodynamics, blunted pressure natriuresis, and sodiumsensitive hypertension. Am. J. Physiol. Regul. Integr. Comp. Physiol., 297: 1358-1363.
PMid:19710386 PMCid:PMC2777774
Posadas SJ, Caz V, Largo C, De la Gándara B, Matallanas B, Reglero G, De Miguel E (2009). Protective effect of supercritical fluid Rosemary extract, Rosmarinus officinalis, on antioxidants of major organs of aged rats. Exp. Gerontol. 44: 383-389.
Rasoulian B, Jafari M, Noroozzadeh A, Mehrani H, Wahhab-Aghai H, Hashemi-Madani SMH, Ghani E, Esmaili M, Asgari A, Khoshbaten A (2008). Effects of ischemia-reperfusion on rat renal tissue antioxidant systems and lipid peroxidation. Acta. Med. Iran, 46: 353-436.
Spurgeon-Pechman KR, Donohoe DL, Mattson DL, Lund H, James L, Basile DP (2007). Recovery from acute renal failure predisposes hypertension and secondary renal disease in response to elevated sodium. Am. J. Physiol. Renal. Physiol., 293: 269-78.
Taddei I, Giachetti D, Taddei E, Mantovani P, Bianchi E (1988). Spasmolytic activity of peppermint. sage and Rosemary essences and their major onstituents. Fitoterapia, 59: 463-8.
Vallon V, Mühlbauer B, Osswald H (2006). Adenosine and kidney function. Physiol. Rev., 86: 901.
Zaouali Y, Bouzaine T, Boussaid M (2010). Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food. Chem. Toxicol., 48: 3144-3152.
Zegura B, Dobnik D, Niderl MH, Filipic M (2011). Antioxidant and antigenotoxic effects of Rosemary (Rosmarinus officinalis L.) extracts in Salmonella typhimurium TA98 and HepG2 cells. Environ. Toxicol. Pharmacol., 32: 296-305.


APA (2012). The study of 24 h post treatment effects of the aqueous extract of Rosmarinus officinalis after renal ischemia/reperfusion in rat. Journal of Physiology and Pathophysiology, 3(2), 12-19.
Chicago Marzieh Zohrabi, Saeed Changizi Ashtiyani, Saeed Hajihashemi, Akbar Hassanpoor and Nasser Hosseini. "The study of 24 h post treatment effects of the aqueous extract of Rosmarinus officinalis after renal ischemia/reperfusion in rat." Journal of Physiology and Pathophysiology 3, no. 2 (2012): 12-19.
MLA Marzieh Zohrabi, et al. "The study of 24 h post treatment effects of the aqueous extract of Rosmarinus officinalis after renal ischemia/reperfusion in rat." Journal of Physiology and Pathophysiology 3.2 (2012): 12-19.

Subscription Form