Journal of Plant Breeding and Crop Science
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Article Number - 2D5501463933


Vol.9(5), pp. 63-70 , May 2017
DOI: 10.5897/JPBCS2016.0630
ISSN: 2006-9758



Full Length Research Paper

Molecular diagnostics of groundnut rosette disease agents in Uganda: Implications on epidemiology and management of groundnut rosette disease



David Kalule Okello
  • David Kalule Okello
  • Department of Groundnut Improvement, National Semi-Arid Resources Research Institute, P. O. Box 56 Soroti, Uganda
  • Google Scholar
Michael Adrogu Ugen
  • Michael Adrogu Ugen
  • Department of Groundnut Improvement, National Semi-Arid Resources Research Institute, P. O. Box 56 Soroti, Uganda
  • Google Scholar
Phinehas Tukamuhabwa
  • Phinehas Tukamuhabwa
  • Department of Agricultural Production, School of Agricultural Sciences, Makerere University, P. O. Box 7062, Kampala, Uganda
  • Google Scholar
Mildred Ochwo-Ssemakula
  • Mildred Ochwo-Ssemakula
  • Department of Agricultural Production, School of Agricultural Sciences, Makerere University, P. O. Box 7062, Kampala, Uganda
  • Google Scholar
Thomas Lapaka Odong
  • Thomas Lapaka Odong
  • Department of Agricultural Production, School of Agricultural Sciences, Makerere University, P. O. Box 7062, Kampala, Uganda
  • Google Scholar
John Adriko
  • John Adriko
  • Biodiversity and Biotechnology Programme, National Agricultural Research Laboratories (NARL), Kawanda, P. O. Box 7065 Kampala, Uganda
  • Google Scholar
Faith Kiconco
  • Faith Kiconco
  • Center for International Tropical Agriculture (CIAT), P. O. Box 6247, Kampala, Uganda
  • Google Scholar
Allan Male
  • Allan Male
  • Center for International Tropical Agriculture (CIAT), P. O. Box 6247, Kampala, Uganda
  • Google Scholar
Carl Michael Deom
  • Carl Michael Deom
  • Department of Plant Pathology, University of Georgia, Athens GA, 30602, USA
  • Google Scholar







 Received: 07 December 2016  Accepted: 20 January 2017  Published: 31 May 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The objective of this study was to use molecular diagnostic tools to detect the agents of groundnut rosette disease (GRD) to guide in varietal development and disease management. Samples were collected from both GRD infected and healthy plants and sites geo-referenced. RNA extraction, cDNA synthesis, polymerase chain reaction (PCR) amplification, electrophoresis, staining and visualization were performed according to standard procedures. Molecular diagnosis of the samples showed various combinations of the GRD agents, some in isolation and others a combination of two or three agents. This distribution is attributed to dependence on the aphid feeding behaviour and pathogenicity of GRD agents. Chlorotic and green rosette symptoms were observed throughout the sampling sites signifying the presence of satellite RNA (sat-RNA) variants. Some plants showing GRD symptoms tested negative for GRD, whereas some healthy-looking plants tested positive for the GRD complexes pointing to the ineffectiveness of phenotypic screening and the need for a molecular diagnostic tool that detects all three GRD agents both in absence or presence of disease symptoms. The absence of groundnut rosette assistor virus (GRAV) in some symptomatic samples signifies that they are epidemiologically dead end sources since GRV and sat-RNA must be packaged within the GRAV coat protein to be aphid transmissible. Oyado (Cassia obtusifolia) tested positive for all the GRD agents making it a potential alternative host. There is an urgent need for validation of the phenotypic screening with molecular tools in efficient diagnosis of the multi-pathogenic GRD in guiding both plant breeding and pathology work.

 

Key words: Arachis hypogaea L., groundnut rosette disease, molecular diagnostics, polymerase chain reaction (PCR).

Anitha S, Monyo ES, Okori P (2014). Simultaneous detection of groundnut rosette assistor virus (GRAV), groundnut rosette virus (GRV) and satellite RNA (satRNA) in groundnuts using multiplex RT-PCR. Arch. Virol. 159(11):3059-3062.
Crossref

 

Ansa OA, Kuhn CW, Misari SM, Demski JW, Casper R, Breyel E (1990). Single and mixed infections of groundnut (peanut) with Groundnut rosette virus and Groundnut rosette assistor virus (abstr.). In F. S. (Ed. ). Arant, ed. Proc Am. Peanut Res Educ Soc 22:40 Arant, F. S. (Ed.). 1951. The Peanut - the Unpredictable Legume: a Symposium. Washington.

 
 

Bock KR, Murant AF, Rajeshwari R (1990). The nature of the resistance in groundnut to rosette disease. Ann. Appl. Biol. 117(2):379-384.
Crossref

 
 

Casper R, Meyer SM, Lesemann DE, Reddy DVR, Rajeswari R, Misari SM, Subbarayudu SS (1983). Detection of a luteovirus in groundnut rosette diseased groundnuts (Arachis hypogaea) by enzyme-linked immunosorbent assay and immunoelectron microscopy. Phytopathol. Z 108:12-17.
Crossref

 
 

Deom CM, Naidu RA, Chiyembekeza AJ, Ntare BR, Subrahmanyam P (2000). Sequence diversity within the three agents of groundnut rosette disease. Phytopathol 90(3):214-219.
Crossref

 
 

Hull R, Adams AN (1968). Groundnut rosette and its assistor virus. Ann. Appl. Biol. 62(1):139-145.
Crossref

 
 

Misari SM, Abraham JM, Demski JW, Ansa OA, Kuhn CW, Casper R, Breyel E (1988) Aphid transmission of the viruses causing chlorotic rosette and green rosette diseases of peanut in Nigeria. Plant Dis. 72:250-253.
Crossref

 
 

Murant AF, Kumar IK (1990). Different variants of the satellite RNA of groundnut rosette virus are responsible for the chlorotic and green forms of groundnut rosette disease. Ann. Appl. Biol. 117(1):85-92.
Crossref

 
 

Murant AF, Rajeshwari R, Robinson DJ, Raschke JH (1988). A Satellite RNA of Groundnut Rosette Virus that is Largely Responsible for Symptoms of Groundnut Rosette Disease. J. Gen.Virol. 69:1479-1486.
Crossref

 
 

Murant AF (1990). Dependence of groundnut rosette virus on its satellite RNA as well as on groundnut rosette assistor luteovirus for transmission by Aphis craccivora. J. Gen. Virol. 71: 2163-2166.
Crossref

 
 

Murant AF (1989). Groundnut rosette assistor virus. CMI\AAB Descriptions of Plant Viruses, No 345.

 
 

Naidu RA, Bottenberg H, Subrahmanyam P, Kimmins FM, Robinson D, Thresh JM (1998a). Epidemiology of groundnut rosette virus disease: current status and future research needs. Ann. Appl. Biol. 132(3):525–548. 
Crossref

 
 

Naidu RA, Robinson DJ, Kimmins FM (1998b). Detection of each of the causal agents of groundnut rosette disease in plants and vector aphids by RT-PCR. J. Virol. Methods. 76:9-18.
Crossref

 
 

Naidu RA, Kimmins FM, Deom CM, Subrahmanyam P, Chiyembekeza AJ, van de Merwe PJ (1999). Groundnut rosette: A virus disease affecting groundnut production in sub-Saharan Africa. Plant Dis. 83(8):700-709.
Crossref

 
 

Naidu RA, Kimmins FM (2007). The Effect of Groundnut rosette assistor virus on the agronomic performance of four groundnut (Arachis hypogaea L.) genotypes. J. Phytopathology 155:350-356.
Crossref

 
 

Okello DK, Akello LB, Tukamuhabwa P, Odong TL, Ochwo-Ssemakula M, Adriko A, Deom CM (2014). Groundnut Rosette Disease Symptoms types distribution and management of the disease in Uganda. Afr. J. Plant Sci. 8(3):153-163.
Crossref

 
 

Okello DK, Biruma M, Deom, CM (2010). Overview of groundnuts research in Uganda : Past, present and future. Afr. J. Biotechnol. 9(39):6448-6459.

 
 

Okusanya BAM, Watson MA (1966). Host range and some properties of groundnut rosette virus. Ann. Appl. Biol. 58(3):377-553.
Crossref

 
 

Olorunju PE, Kuhn CW, Demski JW, Misari SM, Ansa OA (1991). Disease reactions and yield performance of peanut genotypes grown under groundnut rosette and rosette-free field environments. Plant Dis. 75:1269-1273.
Crossref

 
 

Reddy DVR, Murant AF, Duncan GH, Ansa OA, Demski JW, Kuhn CW (1985). Viruses associated with chlorotic rosette and green rosette diseases of groundnut in Nigeria. Ann. Appl. Biol. 107(1):57-64.
Crossref

 
 

Robinson DJ, Ryabov EV, Raj SK, Roberts IM, Taliansky ME (1999). Satellite RNA is essential for encapsidation of groundnut rosette umbravirus RNA by groundnut rosette assistor luteovirus coat protein. Virol. 254(1):104-114.
Crossref

 
 

Scott KP, Farmer MJ, Robinson DJ, Torrance L, Murant AF (1996). Comparison of the coat protein of groundnut rosette assistor virus with those of other luteoviruses. Ann. Appl. Biol. 128:77-83.
Crossref

 
 

Storey HH, Ryland AK (1955). Transmission of groundnut rosette virus. Ann. App. Biol. 43(3):423-432.
Crossref

 
 

Storey HH (1935). Virus Disease of East African Plants: III Rosette Disease of Groundnuts. East Afr. Agric. J. 1(3):206–211.

 
 

Storey HH, Bottomley BA (1928). The rosette disease of peanuts (Arachis hypogaea L.). Ann. Appl. Biol. 15(1):26-45.

 
 

Subrahmanyam P, Hildebrand GL, Naidu RA, Reddy LJ, Singh AK (1998). Sources of resistance to groundnut rosette disease in global groundnut germplasm. Ann. Appl. Biol. 132:473-485.
Crossref

 
 

Taliansky ME, Robinson DJ, Muratnt AF (1996). Complete nucleotide sequence and organization of the RNA genome of groundnut rosette umbravirus. J. Gen. Virol. 77(9):2335-2345.
Crossref

 
 

Waliyar F, Kumar PL, Ntare BR, Monyo E, Nigam SN, Reddy AS, Osiru M, Diallo AT (2007). A Century of Research on Groundnut Rosette Disease and its Management. Information Bulletin no. 75. Technical Report. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andhra Pradesh, India.

 
 

Wangai AW, Pappu SS, Pappu HR, Deom CM, Naidu RA (2001). Distribution and characteristics of groundnut rosette disease in Kenya. Plant Dis. 85(5):470-474.
Crossref

 
 

Zimmerman G (1907). Uber eine Krankheit der Erdnusse (Arachis hypogaea). Pflanzer 3:129-133.

 

 


APA Okello, D. K., Ugen, M. A., Tukamuhabwa, P., Ochwo-Ssemakula, M., Odong, T. L., Adriko, J., Kiconco, F., Male, A., & Deom, C. M. (2017). Molecular diagnostics of groundnut rosette disease agents in Uganda: Implications on epidemiology and management of groundnut rosette disease. Journal of Plant Breeding and Crop Science, 9(5), 63-70.
Chicago David Kalule Okello, Michael Adrogu Ugen, Phinehas Tukamuhabwa, Mildred Ochwo-Ssemakula, Thomas Lapaka Odong, John Adriko, Faith Kiconco, Allan Male and Carl Michael Deom. "Molecular diagnostics of groundnut rosette disease agents in Uganda: Implications on epidemiology and management of groundnut rosette disease." Journal of Plant Breeding and Crop Science 9, no. 5 (2017): 63-70.
MLA David Kalule Okello, et al. "Molecular diagnostics of groundnut rosette disease agents in Uganda: Implications on epidemiology and management of groundnut rosette disease." Journal of Plant Breeding and Crop Science 9.5 (2017): 63-70.
   
DOI 10.5897/JPBCS2016.0630
URL http://academicjournals.org/journal/JPBCS/article-abstract/2D5501463933

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