Journal of Plant Breeding and Crop Science
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Article Number - 9F32F08718


Vol.2(3), pp. 039-043 , March 2010

ISSN: 2006-9758



Estimation of genetic divergence among some cotton varieties by RAPD analysis


L. Chaudhary1*, A. Sindhu, M. Kumar, R. Kumar2 and M. Saini3




1Department of Biotechnology Engineering, Ambala College of Engineering and Applied Research, Mithapur, Ambala Cantt., Haryana, India- 133 101.

2Department of Botany and Plant Physiology, CCS Haryana Agricultural University, Hisar-125 004, India.

3Department of Plant, Soil and Agricultural System, Southern Illinois University, Carbondale-62901, USA.


Email: lakshmi_gpb@rediffmail.com






 Accepted: 05 January 2010  Published: 31 March 2010

Copyright © 2010 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Total genomic DNA from 15 cotton varieties were analysed to evaluate genetic diversity among them through random amplified polymorphic DNA (RAPD) analysis, with 30 random decamer primers using the polymerase chain reaction (PCR). A total of 370 bands were observed, with 12.3 bands per primer, of which 91.6% were polymorphic. OPM-16 produced the maximum number of fragments while the minimum number of fragments was produced with the primer OPM-18. Cluster analysis by the unweighted paired group method of arithmetic means (UPGMA) showed that 15 varieties can be placed in five groups with a similarity ranging from 0.48 - 0.86. Maximum similarity was observed between H-777, H-974 and H-1098 (0.86). Interestingly, these varieties have been developed at one breeding center. The analysis revealed that the intervarietal genetic relationship of several varieties is related to their center of origin. Most of the varieties have a narrow genetic base. These results were well in accordance with previous reported results. The RAPD analysis indicates that it may be a more efficient marker than morphological marker, isozyme and restriction fragment length polymorphism (RFLP) technology. The results obtained can be used in selecting divergent parents for breeding and mapping purposes.

 

Key words: Cluster, cotton varieties, diversity, RAPD, genetic similarities.


APA (2010). Estimation of genetic divergence among some cotton varieties by RAPD analysis. Journal of Plant Breeding and Crop Science, 2(3), 039-043.
Chicago L. Chaudhary, A. Sindhu, M. Kumar, R. Kumar and M. Saini. "Estimation of genetic divergence among some cotton varieties by RAPD analysis." Journal of Plant Breeding and Crop Science 2, no. 3 (2010): 039-043.
MLA L. Chaudhary, et al. "Estimation of genetic divergence among some cotton varieties by RAPD analysis." Journal of Plant Breeding and Crop Science 2.3 (2010): 039-043.
   
DOI
URL http://academicjournals.org/journal/JPBCS/article-abstract/9F32F08718

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