Journal of Plant Breeding and Crop Science
Subscribe to JPBCS
Full Name*
Email Address*

Article Number - BA7A09765719

Vol.9(9), pp. 144-150 , September 2017
ISSN: 2006-9758

 Total Views: 0
 Downloaded: 0

Full Length Research Paper

Estimation of heterosis and combining ability in maize (Zea mays L.) for maize lethal necrosis (MLN) disease

Kiyyo J. G.
  • Kiyyo J. G.
  • Ilonga Agricultural Research Institute, P. O. Box 33 Kilosa, Tanzania.
  • Google Scholar
Kusolwa P. M.
  • Kusolwa P. M.
  • Department of Crop Science and Horticulture, Sokoine University of Agriculture, P. O. Box 3005, Morogoro, Tanzania.
  • Google Scholar

 Received: 21 March 2017  Accepted: 28 June 2017  Published: 30 September 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

Nature of gene action and genetic parameters for disease resistance are important attributes in developing resistant cultivars. This provides the sustainable, economically justifiable and environmentally friendly means of controlling plant diseases. In this study 6 x 6 full diallel cross involving genetically divergent maize inbred lines was performed with the aim of developing resistant cultivars against Maize Lethal Necrosis (MLN) disease under MLN disease hot spot areas in Mlangarini, Ngaramtoni and Kiru six in the Northern Zone of Tanzania during 2015 cropping season. The experimental materials consisted of thirty single cross hybrids, six parents and two local checks. The experiment was laid down in a randomized complete block design (RCBD) with three replications per location. The general combing ability (GCA) and specific combining ability (SCA) effects were significantly different for MLND response among genotypes across all locations. The combined analysis revealed that GCA was highly significant at (P≤0.001) than SCA in all locations with mean squares of (5.551***), (1.61***) and (4.527***) for Mlangarini, Kiru six and Ngaramtoni respectively. The GCA: SCA ratios were 1.894, 1.726 and 1.403 for Mlangarini, Kiru six and Ngaramtoni respectively. The implication of GCA/SCA ratio of more than a unity proves that GCA is significant in all locations where this study was conducted. The results also revealed the presence of both additive and non-additive genetic effects, with the former more pronounced than the later. This implies that developing composite variety will be the better option in combating the disease. However the best cross was observed between CML 144 X CML444 with mean square -0.10, -0.45* and -0.18* for Mlangarini, Kiru six and Ngaramtoni respectively.


Key words: Diallel cross, general combing ability (GCA), heritability, Maize Lethal Necrosis disease (MLND), specific combining ability (SCA), Zea mays.

Ai-Zhi LV, Zhang H, Zhang ZX, Tao YS, Bing YU, Zheng YL (2012). Conversion of the statistical combining ability into a genetic concept. J. Integr. Agric. 11(1):43-52.


Ali F, Yan J (2012). Disease Resistance in Maize and the Role of Molecular Breeding in Defending against Global Threat. J. Integr. Plant Biol. 54:134-151.


DeVries J, Toenniessen G (2001). Securing, the Harvest: Biotechnology, Breeding and Seed Systems for African Crops. The Cromwell Press, Trowbridge, Wiltshire, UK.


Derera J, Tongoona P, Vivek B, VanRij N, Laing M (2007). Gene action determining Phaeosphaerian leaf spot disease resistance in Experimental maize hybrids; African Centre for Crop improvement; University of Kwazulunatal. S. Afr. J. Plant Soil. 24:138-143.


Gomez P, Rodriguez-Hernandez AM, Moury B, Aranda MA (2009). Genetic resistance for the sustainable control of plant virus diseases: Breeding, mechanisms and durability. J. Plant Pathol. 125:1-22.


Griffing B (1956). Concept of general and specific combining ability in relation to diallel crossing systems. Aust. J. Biol. Sci. 9:463-493.


Jawahar RS (2006). Statistical and Biometrical Techniques in Plant Breeding; New International (P) Ltd, Publishers, New Delhi.


Katinila N, Moshi A,Verkuij H, Mwangi W, Anandajagayasekerum P (1998). Adoption of Maize Technologies in Southern Tanzania.International Maize and Wheat Improvement Center (CIMMYT) Mexico. The United Republic of Tanzania and the Southern Centre for Cooperation in Agricultural Research (SACCAR).


Kiani G, Nematzadeh S, Alisha O (2007). Combining ability in cotton cultivars for Agronomic traits. Int. J. Agric. Biol. 9:74-79.


Lapierre H, Signoret P (2004). Viruses and virus diseases of poaceae (gramineae).Paris: INRA Editions.


Mahmoud I, Rashed M, Fahmy E, Abo D (1990). Heterosis, Combining ability and type of gene actions in a 6x6 diallel of maize. The Proceedings of the third conference of Agricultural Development Research, held on 22-24 December, 1990; Cairo, Egypt.


Matt FP, Viana JM (2003). Analysis of general and specific combining abilities of popcorn populations including selfed parents. J. Genet. Mol. Biol. 26(4):465-471.


Patel S, Patel M, Pateland B, Patel J (2014). Combining ability and gene action for grain yield and agronomic traits in pearl millet restorer lines; Elect. J. Plant Breed. 5: 394-401, Gujarat, India.


Redinbaugh MG, Pratt RC (2009).Virus Resistance. In: Bennetzen JL, Hake SC, (eds). Handbook of maize: It's Biology. Springer, New York, pp. 251-268.


Shekhar M, Kumar S (2012). 2nd eds. Inoculation Methods and Disease Rating; Scales for Maize Diseases. Directorate of Maize Research, Indian Council of Agricultural Research, New Delhi.


Sprague GF, Tatum RL (1942). General vs Specific combining ability in single cross of corn. J. Am. Soc. Agron. 34:923-932.


Uyemoto J, Bockelman D, Claflin L (1980). Severe Outbreak of Corn Lethal Necrosis Disease in Kansas. Plant Dis. J. 64:99-100.


Wangai AW, Redinbaugh MG, Kinyua ZM, Miano DW, Leley PK, Kasina M, Mahuku G, Scheets K, Jeffers D (2012). First report of Maize chlorotic mottle virus and Maize Lethal Necrosis in Kenya. Plant Dis. 96:1582-1582.


Water Efficient Maize for Africa (WEMA) project policy brief (2010). Mitigating the impact of drought in Tanzania.



APA Kiyyo, J. G., & Kusolwa, P. M. (2017). Estimation of heterosis and combining ability in maize (Zea mays L.) for maize lethal necrosis (MLN) disease. Journal of Plant Breeding and Crop Science, 9(9), 144-150.
Chicago Kiyyo J. G. and Kusolwa P. M.. "Estimation of heterosis and combining ability in maize (Zea mays L.) for maize lethal necrosis (MLN) disease." Journal of Plant Breeding and Crop Science 9, no. 9 (2017): 144-150.
MLA Kiyyo J. G. and Kusolwa P. M.. "Estimation of heterosis and combining ability in maize (Zea mays L.) for maize lethal necrosis (MLN) disease." Journal of Plant Breeding and Crop Science 9.9 (2017): 144-150.

Subscription Form