Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 180

Full Length Research Paper

Biochemical and antioxidants activity of crude, methanol and n-hexane fractions of Vernonia calvoana on streptozotocin induced diabetic rats

Iwara Arikpo Iwara
  • Iwara Arikpo Iwara
  • Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P. M. B. 1115, Calabar, Nigeria.
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Godwin Oju Igile
  • Godwin Oju Igile
  • Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P. M. B. 1115, Calabar, Nigeria.
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Friday Effiong Uboh
  • Friday Effiong Uboh
  • Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P. M. B. 1115, Calabar, Nigeria.
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Kelvin Ngwu Elot
  • Kelvin Ngwu Elot
  • Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P. M. B. 1115, Calabar, Nigeria.
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Mbeh Ubana Eteng
  • Mbeh Ubana Eteng
  • Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P. M. B. 1115, Calabar, Nigeria.
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  •  Received: 02 December 2016
  •  Accepted: 20 December 2016
  •  Published: 31 March 2017

Abstract

This study is aimed at evaluating the biochemical effects and antioxidants activity of extracts of Vernoia calvoana Hook. f (V.C) on STZ induced diabetic rats. Thirty-six rats weighing (100 to 150 g), were divided into 6 groups of 6 animals each. Groups 1 and 2 representing normal and diabetic controls (NC and DC), respectively, receiving placebo, while groups 3 to 6 represented diabetic treated, receiving 500 mg/kg body weight (b.w.) metformin, 400 mg/kg b.w. crude, n-hexane and methanol fractions of V.C, respectively. Treament with drug and extracts of V.C showed a decrease in fasting blood glucose (FBG) in all experimental groups and was significant (p<0.05) on the 7th day of the experimental period, compared to diabetic control. Progressive increase in body weight was observed in all experimental groups compared to DC group. A significant (p<0.05) increase in glutathione peroxidase (GPX) and catalase (CAT) activities were recorded in all experimental treated animal compared to DC and NC. Malondialdehyde (MDA) concentration was observed to decrease significant (p<0.05) in all experimental groups compared to DC. Histopathologically, the changes in pancreatic integrity were consistent with that of biochemical findings. It may be concluded that, extracts of V.C possess potent ameliorative activity against STZ-induced diabetes, via a potential free radical mopping activity.

 

Key words: Vernonia calvoana, extracts, diabetes mellitus, antioxidants.