Journal of Pharmacognosy and Phytotherapy
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Article Number - 488C71563026


Vol.9(3), pp. 24-34 , March 2017
DOI: 10.5897/JPP2016.0440
ISSN: 2141-2502



Full Length Research Paper

Biochemical and antioxidants activity of crude, methanol and n-hexane fractions of Vernonia calvoana on streptozotocin induced diabetic rats



Iwara Arikpo Iwara
  • Iwara Arikpo Iwara
  • Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P. M. B. 1115, Calabar, Nigeria.
  • Google Scholar
Godwin Oju Igile
  • Godwin Oju Igile
  • Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P. M. B. 1115, Calabar, Nigeria.
  • Google Scholar
Friday Effiong Uboh
  • Friday Effiong Uboh
  • Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P. M. B. 1115, Calabar, Nigeria.
  • Google Scholar
Kelvin Ngwu Elot
  • Kelvin Ngwu Elot
  • Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P. M. B. 1115, Calabar, Nigeria.
  • Google Scholar
Mbeh Ubana Eteng
  • Mbeh Ubana Eteng
  • Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P. M. B. 1115, Calabar, Nigeria.
  • Google Scholar







 Received: 02 December 2016  Accepted: 20 December 2016  Published: 31 March 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


This study is aimed at evaluating the biochemical effects and antioxidants activity of extracts of Vernoia calvoana Hook. f (V.C) on STZ induced diabetic rats. Thirty-six rats weighing (100 to 150 g), were divided into 6 groups of 6 animals each. Groups 1 and 2 representing normal and diabetic controls (NC and DC), respectively, receiving placebo, while groups 3 to 6 represented diabetic treated, receiving 500 mg/kg body weight (b.w.) metformin, 400 mg/kg b.w. crude, n-hexane and methanol fractions of V.C, respectively. Treament with drug and extracts of V.C showed a decrease in fasting blood glucose (FBG) in all experimental groups and was significant (p<0.05) on the 7th day of the experimental period, compared to diabetic control. Progressive increase in body weight was observed in all experimental groups compared to DC group. A significant (p<0.05) increase in glutathione peroxidase (GPX) and catalase (CAT) activities were recorded in all experimental treated animal compared to DC and NC. Malondialdehyde (MDA) concentration was observed to decrease significant (p<0.05) in all experimental groups compared to DC. Histopathologically, the changes in pancreatic integrity were consistent with that of biochemical findings. It may be concluded that, extracts of V.C possess potent ameliorative activity against STZ-induced diabetes, via a potential free radical mopping activity.

 

Key words: Vernonia calvoana, extracts, diabetes mellitus, antioxidants.

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APA Iwara, I. A., Igile, G. O., Uboh, F. E., Elot, K. N., & Eteng, M. U. (2017). Biochemical and antioxidants activity of crude, methanol and n-hexane fractions of Vernonia calvoana on streptozotocin induced diabetic rats. Journal of Pharmacognosy and Phytotherapy, 9(3), 24-34.
Chicago Iwara Arikpo Iwara, Godwin Oju Igile, Friday Effiong Uboh, Kelvin Ngwu Elot and Mbeh Ubana Eteng. "Biochemical and antioxidants activity of crude, methanol and n-hexane fractions of Vernonia calvoana on streptozotocin induced diabetic rats." Journal of Pharmacognosy and Phytotherapy 9, no. 3 (2017): 24-34.
MLA Iwara Arikpo Iwara, et al. "Biochemical and antioxidants activity of crude, methanol and n-hexane fractions of Vernonia calvoana on streptozotocin induced diabetic rats." Journal of Pharmacognosy and Phytotherapy 9.3 (2017): 24-34.
   
DOI 10.5897/JPP2016.0440
URL http://academicjournals.org/journal/JPP/article-abstract/488C71563026

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