Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 186

Full Length Research Paper

Antimicrobial effect of Microdacyn 60, OxOral, and sodium hypochlorite 5.25% in anaerobic bacteria

Elvia Denisse Mena-Mendivil1, Jorge Jaime Flores-Treviño1, Idalia Rodríguez-Delgado1, Myriam A. de la Garza-Ramos1,2*, Hilda H. Torre-Martínez1 and Gustavo Israel Martínez- González1
1Facultad de Odontologia, Universidad Autonoma de Nuevo Leon, Calle Dr. Eduardo Aguirre Pequeño s/n, Colonia Mitras Centro, Monterrey, Nuevo Leon, 64460, Mexico. 2Centro de Investigación y Desarrollo en Ciencias de la Salud, Calle Dr. Carlos Canseco y Ave. Gonalitos s/n, Colonia Mitras Centro, Monterrey, Nuevo Leon, 64460, Mexico.
Email: [email protected]

  •  Accepted: 19 September 2013
  •  Published: 31 October 2013

Abstract

Irrigants have traditionally been used in the root canal. Sodium hypochlorite (NaOCl) is the most commonly used solution, because of its advantages; however, it is known to be toxic to periradicular tissues and can cause necrosis of support tissues and edema. The objective of this study is to compare the antimicrobial effect of Microdacyn 60®, OxOral®, and NaOCl 5.25% against Streptococcus sobrinus, Porphyromona gingivalis, Streptococcus intermedius, Tanerella forsytensis, and Enterococcus faecalis. Thirty-three extracted uniradicular teeth were inoculated with 10 µl of a mixture of anaerobic bacteria. After seven days, the samples were irrigated with the solutions and samples were taken. These were placed in an Eppendorf tube and incubated at 37°C. After seven days, a sample was taken for a bacterial count. Polymerase chain reaction (PCR) was performed to identify the bacteria present in the samples. Student´s t test and analysis of variance were used with a 95% confidence interval. NaOCl showed complete bacteria elimination with no statistically significant difference with OxOral. The saline and Microdacyn 60 groups showed greater bacterial growth than OxOral. In the Microdacyn 60 group, E. faecalis was the most resistant microorganism. NaOCl 5.25% had a greater antibacterial effect, followed by OxOral, and lastly by Microdacyn 60.

 

Key words: Irrigants, sodium hypochlorite (NaOCl), Enterococcus faecalis, Microdacyn 60, OxOral, irrigation.