Journal of Stored Products and Postharvest Research
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Article Number - A2BF99A66534


Vol.8(9), pp. 103-112 , October 2017
DOI: 10.5897/JSPPR2017.0240
ISSN: 2141-6567



Full Length Research Paper

Insecticidal activity of four essential oils on the survival and oviposition of two sympatric bruchid species: Callosobruchus maculatus F. and Callosobruchus subinnotatus PIC. (Coleoptera: Chrysomelidea: Bruchinae)



Seth W. Nyamador
  • Seth W. Nyamador
  • Applied Entomology Laboratory, Faculty of Sciences, University of Lomé, Lomé, Togo.
  • Google Scholar
Abla D. Mondédji
  • Abla D. Mondédji
  • Applied Entomology Laboratory, Faculty of Sciences, University of Lomé, Lomé, Togo.
  • Google Scholar
Boris D. Kasseney
  • Boris D. Kasseney
  • Applied Entomology Laboratory, Faculty of Sciences, University of Lomé, Lomé, Togo.
  • Google Scholar
Guillaume K. Ketoh
  • Guillaume K. Ketoh
  • Applied Entomology Laboratory, Faculty of Sciences, University of Lomé, Lomé, Togo.
  • Google Scholar
Honoré K. Koumaglo
  • Honoré K. Koumaglo
  • Plant Extracts and Natural Aromas Laboratory, Faculty of Sciences, University of Lomé, Lomé, Togo.
  • Google Scholar
Isabelle A. Glitho
  • Isabelle A. Glitho
  • Applied Entomology Laboratory, Faculty of Sciences, University of Lomé, Lomé, Togo.
  • Google Scholar







 Received: 06 March 2017  Accepted: 29 September 2017  Published: 31 October 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Callosobruchus maculatus F. and Callosobruchus subinnotatus Pic. are two pest species of stored cowpeas and bambara groundnuts. Methods of controlling their populations remain the use of chemical insecticides that have ecotoxicological effects. The aim of this work is to look for alternative methods using essential oils extracted from four aromatic plants (Bidens borianiana, Chromolaena odorata, Cymbopogon giganteus and Cymbopogon nardus) to control these pests. Essential oils GC/MS analysis revealed differences in their composition. The major components of the essential oils of the two congeneric Poaceae species C. giganteus and C. nardus are totally different. Limonene (23.03%), cis-p-mentha-2, 8-dien-1-ol (14.26%) and p-mentha-1(7), 8-dien-2-ol isomer (14.06%) were the main compounds in C. giganteus oil whereas citronellal (30.58%) and geraniol (23.93%) were identified in C. nardus oil. In the essential oils of the other two plants, the major components are respectively geyrene (19.44%), α-pinene (15.96%), and germacrene D (14.03%) for Chromolaena odorata essential oil and trans-β-ocimene (31.58%) for Bidens borianiana essential oil. Toxicity tests were performed by fumigation on adult survival and female oviposition in C. maculatus and C. subinnotatus by evaluating the LD10; LD50 and LD90 of the four essential oils. These tests showed that only essential oils extracted from Cymbopogon species are efficient. The essential oil of C. giganteus was the most toxic to adults of both bruchid species. The LD50 were 20.06 and 34.62 µL/L, respectively for C. maculatus and C. subinnotatus while C. nardus essential oil showed the best ovicidal activity with female oviposition reduction in both bruchid species of more than 80% at a lower concentration (10 µL/L). C. giganteus and C. nardus essential oils can thus be used in stocks of cowpea and bambara groundnuts for adult control and prevent female oviposition.

 

Key words: Callosobruchus maculatus, Callosobruchus subinnotatus, Cymbopogon giganteus, Cymbopogon nardus, Bidens borianiana, Chromolaena odorata, essential oils, survival, oviposition

Agboyi LKBA (2009). Vulnérabilité des agroécosystèmes maraîchers du Togo et essai de biocontrôle de deux ravageurs, Brevicoryne brassicae et Plutella xylostella, sur le chou (Brassica oleracea L.), à l'aide de Beauveria bassiana 5653 et de l'extrait aqueux d'amandes de graines de neem (Azadirachta indica A. Juss). Mémoire de DEA, Université de Lomé, 61p.

 

Ahadji-Dabla KM, Brunet JL, Ketoh GK, Apetogbo GY, Glitho AI, Belzunces LP (2015). Larvicidal Activity of a Natural Botanical Biostop Moustiques® and Physiological Changes Induced in Susceptible and Resistant Strains of Anopheles gambiae Giles (Diptera: Culicidae). Open. Entomol. J. 9:12-19.
Crossref

 
 

Ajayi FA, Lale NES (2000). Susceptibility of unprotected seeds and seeds of local bambara groundnut cultivars protected with insecticidal essential oils to infestation by Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). J. Stored Prod. Res. 37:47-62.
Crossref

 
 

Bowers WS, Ortego F, You X, Evans PH (1993). Insect repellents from the Chinese prickly ash Zanthozylum bungueanum. J. Nat. Pro. 56 (6):933-938.
Crossref

 
 

Brink M, Grubben GJH, Brink M, Belay G, Agrooh P (2006). Ressources végétales de l'Afrique tropicale 1 : Céréales et légumes secs. Edition M. Brink. 328p.

 
 

Caburet A, Hekimian Letheve C (2000). Les légumineuses à graines. Memento de l'Agronome pp. 865–878.

 
 

Cassier P, Lafont R, Deschamps M, Porchet M, Soyez D (1997). La reproduction des Invertébrés : Stratégies, modalités et régulation ; Intérêt fondamental et appliqué. Ed. Masson, 354 p.

 
 

Dick KM, Credland PF (1984). Egg production and development of tree strains of Callosobruchus maculatus F. (Coleoptera: Bruchidae). J. Stored Prod. Res. 20:221-227.
Crossref

 
 

Don Pedro KN (1996). Investigation of single and joint fumigant insecticidal action of citruspeel oil components. Pest. Sci. 46:79–84.
Crossref

 
 

Doumma A, Alfari BY, Sembene M, Sidikou SDR, Sanon A, Ketoh GK, Kadidia AH, Glitho IA (2011). Toxicity and persistence of Boscia senegalensis Lam. (Ex Poir.) (Capparaceae) leaves on Callosobruchus maculatus Fab. (Coleoptera:Bruchidae). Int. J. Biol. Chem. Sci. 5(4):1562-1570

 
 

El-nahal AKM, Schmidt GH, Risha EM (1994). Influence of vapours of Acorus calamus L oil on the reproductivity of some stored-product Coleoptera. Pak. J. Entomol. Karachi 9(1):21-27.

 
 

Enan EE (2001). Insecticidal activity of essential oils: Octopaminergic sites of action. Com. Biochem. Physiol. 130:325-337.
Crossref

 
 

FAOSTAT (2007). Agricultural production, crop primary database. Food and Agricultural Organization of the United Nations, Rome. Website at http:// faostat.org (updated 20 January, 2007).

 
 

Finney DJ (1971). Probit analysis. Cambridge University Press, 3rd Edition. 333p.

 
 

Georghiou GP, Lagune Lagunes-Tajeda A (1991). The Occurrence of Resistance to Pesticides in Arthropods. FAO UN Rome. 318p.

 
 

Glitho IA, Ketoh KG, Koumaglo HK (1997). Effets de quelques huiles essentielles sur l'activité reproductrice de Callosobruchus maculatus F. Ann. Univ. Ouagadougou. 5:174-185.

 
 

Hemingway J, Hawkes NJ, McCarroll L, Ranson H (2004). The molecular basis of insecticide resistance in mosquitoes. Insect Biochem. Mol. Biol. 34(7):653-665.
Crossref

 
 

Huignard J, Glitho IA, Sembène M. (2011). L'infestation des cultures puis des stocks de graines par les Coléoptères Bruchinae. In Insectes ravageurs des graines de légumineuses: Biologie des Bruchinae et lutte raisonnée en Afrique. Coord. Huignard J, Glitho IA, Monge J-P, Regnault-Roger C. Ed Quae, France, pp. 33-64.

 
 

Javaid I, Poswal MAT (1995). Evaluation of certain spices for the control of Callosobruchus maculatus (Fabricius) (Coleoptera: Bruchidae) in cowpea seeds. Afr. Entomol. 3(1):87-89.

 
 

Kéita SM, Vincent C, Schmidt JP, Anarson JT, Bélanger A (2001). Efficacy of essential oil of Ocimum basilicum L. and O. graticimum L. applied as an insecticidal fumigant and powder to control Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae). J. Stored Prod. Res. 37(4):339-349.
Crossref

 
 

Ketoh KG (1998). Utilisation des huiles essentielles de quelques plantes aromatiques au Togo comme biopesticides dans la gestion des stades de développement de Callosobruchus maculatus (Coleoptera : Bruchidae). Thèse de Doctorat, Univ. du Bénin, Lomé, 141p.

 
 

Ketoh GK, Glitho IA, Nuto PY (2001). Activité reproductrice de la bruche de voandzou, Callosobruchus subinnotatus Pic (Coleoptera : Bruchidae). J. Rech. Univ. Lomé (Togo) 5(2):159-164.

 
 

Ketoh KG, Glitho IA, Huignard J (2002). Susceptibility of Bruchid Callosobruchus maculatus (Coleoptera: Bruchidae) and its parasitoïde Dinarmus basalis (Hymenoptera: Pteromalidae) to three essential oils. J. Econ. Entomol. 95(1):174-182.
Crossref

 
 

Ketoh KG, Koumaglo HK, Glitho IA, Huignard J (2006). Comparative effects of Cymbopogon schoenanthus essential oil and piperitone on Callosobruchus maculatus developpement. Fitoterapia 77(7- 8):506-510.

 
 

Koumaglo KH, Akpagana K, Glitho IA, Garneau FX, Gagnon H, Jean FI (1996). Germial and Neral, Major Constituents of Lippia multiflora Moldenke Leaf oil. J. Essent. Oil. Res. 8:237-240.
Crossref

 
 

Mazibur MR, Gerhard HS (1999). Effect of Acorus calamus (l.) (Araceae) essential oil vapours from various origins on Callosobruchus phaseoli (Gyllenhal) (Coleoptera: Bruchidae). J. Stored Prod. Res. 35:285-295.
Crossref

 
 

Mondédji AD, Nyamador WS, Amevoin K, Ketoh GK, Glitho AI (2014). Efficacité d'extraits de feuilles de neem Azadirachta indica (Sapindale) sur Plutella xylostella (Lepidoptera : Plutellidae), Hellula undalis (Lepidoptera : Pyralidae) et Lipaphis erysimi (Hemiptera : Aphididae) du chou Brassica oleracea (Brassicaceae) dans une approche « champ école paysan» au sud du Togo. Int. J. Biol. Chem. Sci. 8(5):2286-2295.
Crossref

 
 

Mondédji AD, Nyamador WS, Amevoin K, Abbey GA, Ketoh GK, Philippe Giordanengo, Glitho AI (2015). Treatment and post-treatment effects of neem leaves extracts on Plutella xylostella (Lepidoptera: Plutellidae). Afr. J. Agric. Res. 10(6):472-476.
Crossref

 
 

Negahban M, Moharramipour S, Sefidkon F (2007). Fumigant toxicity of essential oil from Artemisia sieberi Besser against three stored-product insects. J. Stored Prod. Res. 43:123-128.
Crossref

 
 

Ngamo LST, Ngassoum MB, Mapongmetsem PM, Malaisse F, Haubruge E, Lognay G, Hance T (2007). Current post haverst practices to avoid insect attacks on stored grains in Northern Cameroon. Agric. J. 2(2):242-247.

 
 

Nyamador WS, Ketoh GK, Amévoin K, Nuto Y, Kouamaglo HK, Glitho IA (2010). Variation in the susceptibility of two Callosobruchus species to essential oils. J. Stored Prod. Res. 46(1):48-51.
Crossref

 
 

Nyamador SW, Mondedji AD, Ketoh GK, Amevoin K, Glitho IA (2016). Potential Reproductive Activity of Callosobruchus subinnotatus Pic. (Coleoptera: Bruchinae), Bambara Groundnut (Vigna subterranea Verd.) Depredator. J. Life Sci. 10:134-144.

 
 

Odah K (1995). Impact du développement de Callosobruchus maculatus F. (Coleoptera : Bruchidae) sur les graines de trois variétés de Vigna unguiculata Walp. Mémoire de DEA, Univ. Bénin. Lomé. Togo. 65p.

 
 

Papachristos DP, Stamapoulos DC (2004). Fumigant toxicity of tree essential oil vapours on the eggs of Acanthocelides obtectus (Say) (Coleptera: Bruchidae). J. Stored Prod. Res. 40:517–525.
Crossref

 
 

Park Il-K, Lee SG, Choi DH, Park JD, Ahn YJ (2003). Insecticidal activities of constituents identified in the essential oil from leaves of Chamaecyparis obtusa against Callosobruchus chinensis (L.) and Sitophilus oryzae (L.). J. Stored Prod. Res. 39:375–384.
Crossref

 
 

Schmidt GH, Risha EM, El-Nahal AKW (1991). Reduction of progeny of some stored product Coleoptera by vapours of Acorus calamus. J. Stored Prod. Res. 27(2):121-127.
Crossref

 
 

Séri – Kouassi BP (2004). Entomofaune du niébé (Vigna unguiculata L. Walp.) en culture, bio-écologie et gestion des populations de Callosobruchus maculatus Fab. (Coleoptera : Bruchidae) à l'aide d'huiles essentielles comme bio-pesticides dans les stocks de cette denrée en Côte d'Ivoire. Thèse de Doctorat Univ. Cocody. Côte d'Ivoire. 197p.

 
 

Siabi KM (1996). Activité reproductrice de Callosobruchus maculatus F. (Coleoptera : Bruchidae) sur deux variétés de niébé (Vigna unguiculata Walp.). Impact de la valeur marchande du niébé au Togo. Mémoire d'Ingénieur Agroalimentaire. Univ. Bénin. Lomé. Togo. 63p.

 
 

Tabashnik BE (1994). Evolution of resistance to Bacillus thuringiensis. Annu. Rev. Entomol. 39:47-79.
Crossref

 

 


APA Nyamador, S. W., Mondédji, A. D. , Kasseney, B. D., Ketoh, G. K., Koumaglo, H. K. & Glitho, I. A. (2017). Insecticidal activity of four essential oils on the survival and oviposition of two sympatric bruchid species: Callosobruchus maculatus F. and Callosobruchus subinnotatus PIC. (Coleoptera: Chrysomelidea: Bruchinae). Journal of Stored Products and Postharvest Research, 8(9), 103-112.
Chicago Seth W. Nyamador, Abla D. Mond&edji, Boris D. Kasseney, Guillaume K. Ketoh, Honor&e K. Koumaglo and Isabelle A. Glitho. "Insecticidal activity of four essential oils on the survival and oviposition of two sympatric bruchid species: Callosobruchus maculatus F. and Callosobruchus subinnotatus PIC. (Coleoptera: Chrysomelidea: Bruchinae)." Journal of Stored Products and Postharvest Research 8, no. 9 (2017): 103-112.
MLA Seth W. Nyamador, et al. "Insecticidal activity of four essential oils on the survival and oviposition of two sympatric bruchid species: Callosobruchus maculatus F. and Callosobruchus subinnotatus PIC. (Coleoptera: Chrysomelidea: Bruchinae)." Journal of Stored Products and Postharvest Research 8.9 (2017): 103-112.
   
DOI 10.5897/JSPPR2017.0240
URL http://academicjournals.org/journal/JSPPR/article-abstract/A2BF99A66534

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