Journal of Soil Science and Environmental Management
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Article Number - CC934B367063


Vol.8(11), pp. 190-197 , December 2017
https://doi.org/10.5897/JSSEM2017.0657
ISSN: 2141-2391


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Full Length Research Paper

Assessment of the ecological state of soil above an underground natural gas storage using bioassay



Clement T. Ngun
  • Clement T. Ngun
  • Department of Biochemistry and Biophysics, Saratov State University, Saratov, 410012, Russia.
  • Google Scholar
Yekaterina V. Pleshakova
  • Yekaterina V. Pleshakova
  • Department of Biochemistry and Biophysics, Saratov State University, Saratov, 410012, Russia.
  • Google Scholar
Mihail V. Reshetnikov
  • Mihail V. Reshetnikov
  • Laboratory of Geoecology and Ecological Geochemistry, Saratov State University, Saratov, 410012, Russia.
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 Received: 14 September 2017  Accepted: 24 November 2017  Published: 31 December 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Soils in areas of underground natural gas storage facilities (UGS) can become contaminated with hydrocarbons, which impacts negatively on soil properties and functions of soil biocenosis. The purpose of this study was to biodiagnose soil samples from the territory of an UGS (Stepnoye district, Saratov region) using bioassay. For microbiological analysis of the soil samples, the following parameters were selected: The total number of heterotrophic microorganisms, the content of hydrocarbon-oxidizing and methylotrophic bacteria, as well as the number of iron-oxidizing bacteria given that in the area of this natural gas storage facility clusters of bacteriomorphic magnetite may have been formed. Dehydrogenase, catalase and β-fructofuranosidase activities were studied as informative and sensitive indicators. The biodiagnostic results showed reduced levels of heterotrophic microorganisms in the soil samples from the area of this UGS compared to the background samples obtained outside the UGS. A high content of indicator organisms: Hydrocarbon-oxidizing and methylotrophic bacteria, not only facultative, but obligate was observed in some soil samples which suggests the emission of methane into the upper layers of the soil. The presence of iron-oxidizing microorganisms in the soil samples was confirmed by an increased activity of dehydrogenases and catalases. The observed correlations between the studied biological and physico-chemical parameters of soil confirmed the presence of hydrocarbon pollution.

 

Key words: Underground gas storage, bioassay, soil microorganisms, soil enzymes, magnetic susceptibility.

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APA Ngun, C. T., Pleshakova, Y. V., & Reshetnikov, M. V. (2017). Assessment of the ecological state of soil above an underground natural gas storage using bioassay. Journal of Soil Science and Environmental Management, 8(11), 190-197.
Chicago Clement T. Ngun, Yekaterina V. Pleshakova and Mihail V. Reshetnikov. "Assessment of the ecological state of soil above an underground natural gas storage using bioassay." Journal of Soil Science and Environmental Management 8, no. 11 (2017): 190-197.
MLA Clement T. Ngun, Yekaterina V. Pleshakova and Mihail V. Reshetnikov. "Assessment of the ecological state of soil above an underground natural gas storage using bioassay." Journal of Soil Science and Environmental Management 8.11 (2017): 190-197.
   
DOI https://doi.org/10.5897/JSSEM2017.0657
URL http://academicjournals.org/journal/JSSEM/article-abstract/CC934B367063

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