Journal of Toxicology and Environmental Health Sciences
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Article Number - 00F65A564804


Vol.9(6), pp. 59-65 , June 2017
DOI: 10.5897/JTEHS2017.0381
ISSN: 2006-9820



Full Length Research Paper

Maternal and foetal toxicity induced by exposure to mixture of dimethoate and cypermethrin in albino rats



S. B. Ramon-Yusuf
  • S. B. Ramon-Yusuf
  • National Universities Commission, Maitama, Abuja, Nigeria.
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Y. O. Aliu
  • Y. O. Aliu
  • Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria.
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O. A. Salawu
  • O. A. Salawu
  • National Institute of Pharmaceutical and Research Development, Idu, Abuja, Nigeria.
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I. Chahoud
  • I. Chahoud
  • WHO Collaborating Centre for Developmental Toxicology, Institute of Clinical Pharmacology and Toxicology, Campus Benjamin Franklin, Charité University Medical School, Berlin, Germany.
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S. F. Ambali
  • S. F. Ambali
  • Department of Veterinary Pharmacology and Toxicology, University of Ilorin, Ilorin, Kwara State, Nigeria.
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 Received: 26 January 2017  Accepted: 03 April 2017  Published: 30 June 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The use of pesticide mixture to combat increased resistance by pest, to single chemical insecticide has brought about an increase in the use of pesticide mixture from different class, with its attendant toxicity. The objective of this study was to evaluate the maternal and foetal toxicity associated with dimethoate and cypermethrin, the two insecticides that are normally mixed to reduce pest resistance. 100 confirmed-mated (gravid) females were assigned at random among the four groups (control – tap water only):   group 1- very low dose-10 mg/kg/day of Cypermethrin (5.3% LD50) + 1.0 mg/kg/day Dimethoate (0.7% of LD50); group 2- intermediate dose- 19 mg/kg/day of Cypermethrin (10% LD50) + 1.4 mg/kg/day  Dimethoate (0.9% of LD50); and group 3- high dose-  38 mg/kg/day of Cypermethrin (20% LD50) + 12.8 mg/kg/day  Dimethoate (8.5% of LD50). The regimens were administered orally at gestation days (GD) 6-20 to pregnant rats. The dams were examined for mortality, moribundity, pertinent behavioural changes and signs of overt toxicity. Animals were weighed on day 0, and once at every 3-day intervals during the dosing period and on GD 21. The pregnant rats were sacrificed by decapitation on GD 21, the uteri were removed and the fetuses examined for litter weight, size, resorption, sex ratio, absolute and relative organ weight. The results showed that combined pesticide exposure caused overt toxic signs in the dams. However, the maternal body weight and relative organ weight were not adversely affected except in the heart that showed significant alteration. Although, the litter weight was not adversely compromised, the result showed that the prenatal exposure to pesticide mixture caused increased foetal resorption, decreased the litter size and offsprings’ male/female ratio. The study concludes that prenatal co-exposure to cypermethrin and dimethoate has adverse consequences on some maternal and foetal parameters in albino rats.

Key words: Pesticide mixture, litter size, sex ratio, litter weight, maternal relative organ weight.

Abouamer W, Abu-Shaeir W, Bakry S (2013). Dimethoate Induced Intrauterine Growth Retardations in Mice. American-Eurasian J. Toxicol. Sci. 5(4):85-93.

 

Ahmad L, Khan A, Khan MZ (2011). Cypermethrin induced biochemical and hepato-renal pathological changes in rabbits. Int. J. Agric. Biol.13: 865-872.

 

Al-Hamdani NMH, Yajurvedi HN (2010). Cypermethrin reversibly alters sperm count without altering fertility in mice. Ecotoxicol. Environ. Safety 73:1092-1097.
Crossref

 

Ambali SF, Ayo JO (2011). Sensorimotor performance deficits induced by chronic chlorpyrifos exposure in Wistar rats: mitigative effect of vitamin C. Toxicol. Environ. Chem. 93:1212-1226
Crossref

 

Assayed ME, Khalaf AA, Salem HA (2010). Protective effects of garlic extract and vitamin C against in vivo cypermethrin-induced teratogenic effects in rat offspring. Food Chem. Toxicol. 48(11):3153-3158.
Crossref

 

Astolfi P, Zonta LA (1999). Reduced male births in major Italian cities. Hum. Reprod. 14:3116-3119.
Crossref

 

Audegond L, Catez D, Foulhoux R, Le Rumeur C, L'Hotelier M, Stepniowski JP (1988). Protection of deltamethrin toxicity by organophosphorous insecticides. Journal de toxicologie clinique et experimentale 9(3):163-176.

 

Bhaskar N, Shahani L, Bhatnagar P (2014). Morphological and Skeletal Abnormalities Induced by Commercially Available Insecticides Colonel-s® and Decis® in the Developing Embryo of Gallus domesticus. Int. J. Pharm. Sci. Rev. Res. 26(1):140-148.

 

Colborn T (2006). A case for revisiting the safety of pesticides: a closer look at Neurodevelopment. Environ. Health Perspect. 114:10-17.
Crossref

 

Cox C (1996). Insecticide fact sheet - Cypermethrin. J. Pestic. Reform. 16(2):15-20.

 

Cremonese C, Freire C, De Camargo AM, De Lima JS, Koifman S, Meyer A (2014). Pesticide consumption, central nervous system and cardiovascular congenital malformations in the South and Southeast region of Brazil. Int . J. Occup. Med. Environ.Health 27(3):474-486.
Crossref

 

Crumpton WG (2001). Using wetlands for water quality improvement in agricultural wetlands the importance of water shed sale approach. Water Sci. Tech 44(11-12):559-564.

 

Dewailly E, Forde M, Robertson L, Kaddar N, Laouan SEA, Côté S, Gaudreau E, Drescher O, Ayotte P (2014). Evaluation of pyrethroid exposures in pregnant women from 10 Caribbean countries. Environ. Int. 63:201-206.
Crossref

 

Farag AT, EL Okazy AM, El-Sebael AK (2003). Developmental toxicity study of chlorpyriphos in rats. Reprod. Toxicol. 17:2003-2008.
Crossref

 

Farag AT, Eweidah MH, EL Okazy AM (2000). Reproductive toxicology of acephate in male mice. Reprod. Toxicol. 14:457-462.
Crossref

 

Farag AT, Karkour T, El Okazy A (2006). Teratogenic effect of orally administered technical dimethoate in rats. Birth Defects Res. Part B: Dev. Reprod. Toxicol. 77:40-46.
Crossref

 

Freeman N, Shalat S, Black K, Jimenez M, Donnelly K, Calvin A, Ramirez J (2004). Seasonal pesticide use in a rural community on the US/Mexico border. J. Expo. Anal. Environ. Epidemiol. 14:473-478.
Crossref

 

Gabr GA, Soliman GA, Abdulaziz SS, Al- Kahtani AA, Ali BE (2015). Teratogenic Effects in rat foetuses subjected to the concurrent in utero exposure to emamectin benzoate insecticide. Pak. J. Biol. Sci. 18:333-340.
Crossref

 

Gallo MA, Lawryk NJ (1991). Organic phosphorus pesticides. In Handbook of Pesticide Toxicology. Hayes WJ Jr., Laws ER Jr, Eds. Academic Press, New York, NY. pp. 5-3.

 

Garber JC, Barbee RW, Bielitzki JT, Clayton LA, Donovan JC, Hendriksen CF, Kohn DF, Lipman NS, Locke PA, Melcher J, Quimby FW (2011). Guide for the care and use of laboratory animals. The National Academic Press, Washington DC. 8:220.

 

Garcia AM, Fletcher T, Benevides FG, Orts E (1999). Parental agricultural work and selected congenital malformations. Am. J. Epidemiol. 149:64-74.
Crossref

 

Garey J, Wolff MS (1998). Estrogenic and antiprogestagenic activities of pyrethroid insecticides. Biochem. Biophys. Res. Commun. 251:855-859.
Crossref

 

Garry VF, Harkins M, Lyubimov A, Erickson L, Long L (2002). Reproductive outcomes in the women of the red river valley of the North. I. The spouses of pesticide applicators: Pregnancy loss, age at menarche, and exposures to pesticides. J. Toxicol. Environ. Health Part A 65:769-786.
Crossref

 

Gary LE, Ostby J (1998). Effects of pesticides and toxic substances on behavioral and morphological reproductive mechanisms. Toxicol. Ind. Health 14:159-184.
Crossref

 

Glass R (2008). Chronic and long-term effects of pesticides use in agriculture: current knowledge and limits. Toxicol. Lett. 180:S21.
Crossref

 

Gomes J, Dawodu AH, Lloyd O, Revitt DM, Anilal SV (1999a). Hepatic injury and disturbed amino acid metabolism in mice following prolonged exposure to organophosphorous. Hum. Exp.Toxicol. 18:33-37.
Crossref

 

Gomes J, Lloyd O, Rebvitt MD, Basha M (1999b). Morbidity among farm workers in a country in relation to long term exposure to pesticides. Scand. J. Work Environ. Health 24:213-219.
Crossref

 

Gomes J, Lloyd OL, Hong Z (2008). Oral exposure of male and female mice to formulations of organophosphorous pesticides: Congenital malformations. Hum. Exp. Toxicol. 27:231-240.
Crossref

 

Gore AC (2001). Environmental toxicant effects on neuroendocrine function. Endocrine 14:235-246.
Crossref

 

Huang C, Li X (2014). Maternal cypermethrin exposure during the perinatal period impairs testicular development in C57BL male offspring. PLOS 9(5):96781.
Crossref

 

International Programme on Chemical Safety (IPCS/WHO) (1992). Dimethoate Health and Safety Guide. Health and Safety Guide P 20.

 

Kimberly PM, Borgeest C, Greenfeld DT, Flaws JA (2004). In utero effects of chemicals on reproductive tissues in females. Toxicol. Appl. Pharmacol. 198(2):111-131.
Crossref

 

Landrigan PJ, Claudio L, Markowitz SB, Berkowitz GS, Brenner BL, Romero H, Wetmur JG, Matte TD, Gore AC, Godbold JH, Wolff MS (1999). Pesticides and inner-city children: exposures, risks, and prevention. Environ. Health Perspect. 107(3):431-437.
Crossref

 

Liu P, Song X, Yuan W, Wen W, Wu X, Li J, Cheu X (2006). Effects of cypermethrin and methyl parathion mixtures on hormone levels and immune function in Wister rats. Arch. Toxicol. 80:449-457.
Crossref

 

Madu EP(2015).Teratogenic and embryotoxic effects of orally administered cypermethrin in pregnant albino rats. J. Toxicol. Environ. Health Sci. 7(7):60-67.
Crossref

 

Marinovich M, Ghilardi F, Galli CL (1996). Effects of mixtures on in vitro nervous cells: comparison with single pesticides. Toxicology108:201-206.

 

Martin T, Ochou OG, Vaissayre M, Fournier D (2003). Organophosphorous insecticides synergize pyrethroids in the resistant strain of cotton bellworm, Helicoverpa armigera (Hubner) (Lepidoptera Noctuidae) from West Africa. J. Econ. Entomol. 96(2):468-474.

 

Martuzzi M, Di Tanno ND, Bertollini R (2001). Declining trends of male proportion at birth in Europe. Arch. Environ. Health 56:358-364.
Crossref

 

Matt DW, Borzelleca JF (1995). Toxic effects on the female reproductive system during pregnancy,parturition and lactation. In: Witorsch, Raphael J. (Ed.), Reproductive Toxicology, second ed. Raven Press Ltd. New York, NY, USA. pp. 175-193.

 

Narahashi T (1996). Neuronal ion channels as the target sites of insecticides. Pharmacol. Toxicol. 78:1-14.
Crossref

 

National Agricultural Statistics Service (2005). NASS Pesticide use Data. Available: http://old.ipmcenters.org/datasources/nass/

 

Olgan S, Gogal RM, Adeshina F, Choudhry H, Misra HP (2004). Pesticide mixtures potentiate the toxicity in murine thymocytes. Toxicology 196:181-196.
Crossref

 

Raees K, Asmatullah, Ahmad KR (2010). Pregnancy and foetal correlations of cypermethrin in mice (Mus musculus). Biologia 56(1&2):39-54.

 

Roberts T, Hutson D (1999). Metabolic Pathways of Agrochemicals. Part 2: Insecticides and Fungicides. The Royal Society of Chemistry, Cambridge, United Kingdom. pp. 1180-1384.
Crossref

 

Sarkar R, Maitra SK (1990). Responses of adrenal medulla to oral administration of organophosphorus pesticides in roseringed parakeets Psittacula krameri borealis (Neumann). Euro. Arch. Biol. 101:469-480.

 

Shaw GM, Wasserma CR, O'Marley CD, Nielsen V, Jackson RJ (1999). Maternal pesticide exposures from multiple sources and selected congenital anomalies. Epidemiology 10:60-66.
Crossref

 

Singh AK, Tiwari MN, Prakash O, Singh MP (2012). A current review of cypermethrin-induced neurotoxicity and nigrostriatal dopaminergic neurodegeneration. Curr. Neuropharmacol 10(1):64-71.
Crossref

 

Sinha C, Shukla, GS (2003). Species variation in pesticide induced blood-brain barrier dysfunction. Hum. Exp. Toxicol. 22:647-652
Crossref

 

Srivastava MK, Raizada RB (1996). Development toxicity of technical dimethoate in rats: maternal and foetal toxicity evaluation. Ind. J. Exp. Biol. 34(4) 329-333.

 

Stillerman KP, Mattison DR, Giudice LC, Woodruff TJ (2008). Environmental exposures and adverse pregnancy outcomes: A review of the science. Reprod. Sci. 15:631-650.
Crossref

 

Syed F, Soni I, John PJ, Bhatnagar P (2009). Embryotoxic and teratogenic evaluation of cyfluthrin in Swiss albino mice. Toxicol. Int. 16:121-126.

 

WHO (1989). Environmental Health Criteria Monograph "Dimethoate" World Health Organization. Geneva.

 

Young JG, Eskenazi B, Gladstone EA, Bradham A, Pedersen L, Johnson C (2006). Association between in-utero organophosphate pesticide exposure and abnormal reflexes in neonates. Neurotoxicology 26:199-209.
Crossref

 


APA Ramon-Yusuf, S. B., Aliu, Y. O., Salawu, O. A., Chahoud, I., & Ambali, S. F. (2017). Maternal and foetal toxicity induced by exposure to mixture of dimethoate and cypermethrin in albino rats. Journal of Toxicology and Environmental Health Sciences, 9(6), 59-65.
Chicago S. B. Ramon-Yusuf, Y. O. Aliu, O. A. Salawu, I. Chahoud and S. F. Ambali. "Maternal and foetal toxicity induced by exposure to mixture of dimethoate and cypermethrin in albino rats." Journal of Toxicology and Environmental Health Sciences 9, no. 6 (2017): 59-65.
MLA S. B. Ramon-Yusuf, et al. "Maternal and foetal toxicity induced by exposure to mixture of dimethoate and cypermethrin in albino rats." Journal of Toxicology and Environmental Health Sciences 9.6 (2017): 59-65.
   
DOI 10.5897/JTEHS2017.0381
URL http://academicjournals.org/journal/JTEHS/article-abstract/00F65A564804

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