Journal of Toxicology and Environmental Health Sciences
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Article Number - 029903B4769


Vol.5(9), pp. 166-171 , September 2013
DOI: 10.5897/JTEHS12.029
ISSN: 2006-9820



Full Length Research Paper

Effect of some pesticides on growth, nitrogen fixation and nifgenes in Azotobacter chroococcum and Azotobacter vinelandii isolated from soil



Kasim Abass Askar
  • Kasim Abass Askar
  • School of Biomedical and Biological Sciences, University of Plymouth, Plymouth, PL4 8AA, United Kingdom
  • Google Scholar
Aras Mohammed Khudhur
  • Aras Mohammed Khudhur
  • Soil and Water Department, College of Agriculture, University of Salahaddin, Erbil, Iraq
  • Google Scholar







 Accepted: 30 July 2013  Published: 30 September 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


This study was designed to evaluate the effects of three pesticides (Imazetapir, Dimethoate and Bayleton 50) at the recommended concentration (in the field), on the growth of pure cultures ofAzotobacter chroococcum and Azotobacter vinelandii, on the amount of fixed nitrogen and nifgenes. Herbicide Imazetapir had no negative effect on nitrogen fixing bacteria, while Dimethoate and Bayleton 50 exhibited inhibitory effect on growth. Same effects were obtained on fixed nitrogen obtained when treated with studied pesticides. nifH1, nifH2, nifH3, nifU and nifV from A. chroococcum, and nifH, nifK, nifD, and nifM gene in A. Vinelandii were lost when pots were cultivated with wheat and treated with both Dimethoate and Bayleton 50, therefore, be deemed highly susceptible to them. While herbicide did not affect the nif genes, the bands on gel electrophoresis appeared as normal sample.

 

Key words: Azotobacter chroococcumAzotobacter vinelandii, fungicide (Bayleton 50), herbicide (Imazetapir), insecticide (Dimethoate).

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APA (2013). Effect of some pesticides on growth, nitrogen fixation and nifgenes in Azotobacter chroococcum and Azotobacter vinelandii isolated from soil. Journal of Toxicology and Environmental Health Sciences, 5(9), 166-171.
Chicago Aras Mohammed Khudhur, and Kasim Abass Askar. "Effect of some pesticides on growth, nitrogen fixation and nifgenes in Azotobacter chroococcum and Azotobacter vinelandii isolated from soil." Journal of Toxicology and Environmental Health Sciences 5, no. 9 (2013): 166-171.
MLA Aras Mohammed Khudhur, and Kasim Abass Askar. "Effect of some pesticides on growth, nitrogen fixation and nifgenes in Azotobacter chroococcum and Azotobacter vinelandii isolated from soil." Journal of Toxicology and Environmental Health Sciences 5.9 (2013): 166-171.
   
DOI 10.5897/JTEHS12.029
URL http://academicjournals.org/journal/JTEHS/article-abstract/029903B4769

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