Journal of Toxicology and Environmental Health Sciences
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Article Number - 2F1C8C1793


Vol.2(1), pp. 1-6 , April 2010

ISSN: 2006-9820



Full Length Research Paper

Effect of Hibiscus sabdariffa anthocyanins on 2, 4-dinitrophenylhydrazine-induced tissue damage in rabbits


A. Ologundudu1*, A. O. Ologundudu1, O. M. Oluba2, I. O. Omotuyiand F. O. Obi2 




Department of Biochemistry, Adekunle Ajasin University, Akungba Akoko, Ondo State, Nigeria.


Email: oluologundudu@yahoo.com






 Accepted: 08 December 2009  Published: 30 April 2010

Copyright © 2010 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


This study examines the effects of anthocyanin extract of the dried calyces of Hibiscus sabdariffa Linnon the 2, 4 -dinitrophenylhydrazine (2, 4 -DNPH)-induced cytotoxic effects in rabbits. Twenty male adult rabbits used for the study were divided into four groups. Group 1, the control took only water while animals in groups 2 and 4 received 100 mg/kg body weight of the anthocyanin extract of H. sabdariffa once daily for 28 days. After the 22nd day of treatment, the rabbits in groups 3 and 4 received 28 mg/kg body weight of DNPH for the remaining 5 days of treatment, after which the animals were sacrificed. Relative to control, DNPH caused significant (p < 0.05) increase in the formation of malondialdehyde (MDA) in serum, liver and brain and decreased the levels of reduced glutathione (GSH) in liver and brain. Also, DNPH caused a significant (p < 0.05) elevation in the activity of glucose- 6 -phosphate dehydrogenase (G6PD) in the serum and liver. However, pretreatment with H. sabdariffa anthocyanin extract significantly (p < 0.05) reduced MDA formation, increased the levels of GSH and maintained at normalcy the activity of G6PD in the tissues, thereby effectively ameliorated the toxic effects of DNPH. These findings indicate that anthocyanin extract from dried calyces of H. sabdariffa protects the rabbit against 2, 4 -DNPH lipoperoxidative and cytotoxic effects.

 

Key words: Anthocyanin extract, 2, 4-dinitrophenylhydrazine, glucose-6-phosphate dehydrogenase, Hibiscus sabdariffa, rabbit, reduced glutathione, malondialdehyde


APA (2010). Effect of Hibiscus sabdariffa anthocyanins on 2, 4-dinitrophenylhydrazine-induced tissue damage in rabbits. Journal of Toxicology and Environmental Health Sciences, 2(1), 1-6.
Chicago A. Ologundudu, A. O. Ologundudu, O. M. Oluba, I. O. Omotuyi and F. O. Obi . "Effect of Hibiscus sabdariffa anthocyanins on 2, 4-dinitrophenylhydrazine-induced tissue damage in rabbits." Journal of Toxicology and Environmental Health Sciences 2, no. 1 (2010): 1-6.
MLA A. Ologundudu, et al. "Effect of Hibiscus sabdariffa anthocyanins on 2, 4-dinitrophenylhydrazine-induced tissue damage in rabbits." Journal of Toxicology and Environmental Health Sciences 2.1 (2010): 1-6.
   
DOI
URL http://academicjournals.org/journal/JTEHS/article-abstract/2F1C8C1793

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