Journal of Toxicology and Environmental Health Sciences
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Article Number - 9E7CA0667152


Vol.9(10), pp. 92-97 , December 2017
DOI: 10.5897/JTEHS2017.0391
ISSN: 2006-9820



Full Length Research Paper

Effects of contaminated natural soil by Glyphosan SL on biochemical responses of the earthworm Eisenia sp.



Leida Marcano
  • Leida Marcano
  • Departamento de Biología, Universidad de Oriente, Escuela de Ciencias, Cumaná-Venezuela.
  • Google Scholar
Juanny Hernandez
  • Juanny Hernandez
  • Postgrado Biología Aplicada, Departamento de Biología, Universidad de Oriente, Cumaná-Venezuela.
  • Google Scholar
Edgar Zapata-Vivenes
  • Edgar Zapata-Vivenes
  • Departamento de Biología, Universidad de Oriente, Escuela de Ciencias, Cumaná-Venezuela.
  • Google Scholar
Analia Leon
  • Analia Leon
  • Departamento de Biología, Universidad de Oriente, Escuela de Ciencias, Cumaná-Venezuela.
  • Google Scholar







 Received: 14 April 2017  Accepted: 29 June 2017  Published: 31 December 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Biochemical biomarkers are widely used for the monitoring environmental quality since they can act as early warning signals, for potential ecosystem degradation caused by contaminants. In order to investigate the acute and chronic effects of a commercial formulation of glyphosate on antioxidant defenses (glutathione peroxidase, GPx; glutathione-S-transferase, GST; and reduced glutathione, GSH) and oxidative damage (malondialdehyde levels, MDA), Eisenia sp was exposed in plastic containers to natural soils contaminated with Glyphosan SL (100 g kg-1 of soil) during 7 and 21 days. Following exposure for 21 days, another group of worms were placed on herbicide-free soils to recovery for 30 days. Treatment with the herbicide significantly affected to all the biochemical markers evaluated. Antioxidant defenses (GSH, GPx and GST) and MDA, in general, increased at both exposure periods. These results showed that Glyphosan SL; the active ingredient (glyphosate) and/or its chemical additives exerts its toxic effects in Eisenia sp by altering cellular antioxidant defenses, inducing a condition of oxidative stress. During the recuperation phase of the earthworms previously exposed for 21 days to the herbicide, GPx decreased to values similar to those of the control group and MDA although decreased, control values were not reached. These results indicate that in 30 days Eisenia sp exhibits a partial recovery of the oxidative stress induced by the herbicide exposure, probably the activation of mechanisms clearance of the Glyphosan SL metabolites justify the high levels of GSH and GST at this stage. In conclusion, herbicide exposure induced changes in the biochemical responses of Eisenia sp highlight the importance of these responses as useful tools in the evaluation of impacts by pesticides in terrestrial organisms.
 
Key words: Biomarker, Eisenia, glyphosate, antioxidant enzymes, glutathione, lipid peroxidation.

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APA Marcano, L., Hernández, J., Zapata-Vívenes, E., & León, A. (2017). Effects of contaminated natural soil by Glyphosan SL on biochemical responses of the earthworm Eisenia sp.. Journal of Toxicology and Environmental Health Sciences, 9(10), 92-97.
Chicago Leida Marcano, Juanny Hern&andez, Edgar Zapata-V&ivenes and Anal&ia Le&on. "Effects of contaminated natural soil by Glyphosan SL on biochemical responses of the earthworm Eisenia sp.." Journal of Toxicology and Environmental Health Sciences 9, no. 10 (2017): 92-97.
MLA Leida Marcano, et al. "Effects of contaminated natural soil by Glyphosan SL on biochemical responses of the earthworm Eisenia sp.." Journal of Toxicology and Environmental Health Sciences 9.10 (2017): 92-97.
   
DOI 10.5897/JTEHS2017.0391
URL http://academicjournals.org/journal/JTEHS/article-abstract/9E7CA0667152

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