Journal of Toxicology and Environmental Health Sciences
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Article Number - BE64F7B754


Vol.1(1), pp. 001-007 , June 2009

ISSN: 2006-9820



Full Length Research Paper

Characterization of industrial waste and identification of potential micro-organism degrading tributyl phosphate


Trupti D. Chaudhari3, Susan Eapen2 and M. H. Fulekar1*




Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai-400 098, India.


Email: mhfulekar@yahoo.com






 Accepted: 06 May 2009  Published: 30 June 2009

Copyright © 2009 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The present research study has been carried out in the waste disposal site for characterization of physical, chemical and biological parameters to assess the microbial consortium present in the contaminated site and to isolate the potential microorganism for biodegradation of Tributyl Phosphate. The ambient conditions present in the contaminated site shows the values: pH (6.61), Temperature (35.6), Moisture (50.72%), Nutrients; Nitrogen (0.41%), Phosphorus (27.87 mg/l), and Sulphur (993.5 mg/l) respectively. The biological parameters studied indicate Dissolved Oxygen (4.58 mg/l), Biological Oxygen Demand (4.62 mg/l), Chemical Oxygen Demand (146.1 mg/l). The microbial consortium identified was found to survive and multiply in the present environment conditions. Microbial consortium was sequenced and compared using BLAST, ClustalW and PHYLIP. In order to identify potential microorganism, microbial consortium was exposed to increasing concentration of Tributyl Phosphate viz. 10, 25, 50, 75, 100 mg/l in MSM, the potential microorganism was found to survive at higher concentration and utilized it as a sole source of carbon. This organism was   identified as Pseudomonas pseudoalcaligenes strain DSM 50018T using 16S rRNA sequencing. This organism was found to have high potential for degradation of Tributyl Phosphate present in Low Level Nuclear Waste.

 

Key words: Tributyl phosphate (TBP), low level nuclear waste, 16S rRNA sequencing, industrial effluent, biodegradation.


APA (2009). Characterization of industrial waste and identification of potential micro-organism degrading tributyl phosphate. Journal of Toxicology and Environmental Health Sciences, 1(1), 001-007.
Chicago Trupti D. Chaudhari, Susan Eapen and M. H. Fulekar. "Characterization of industrial waste and identification of potential micro-organism degrading tributyl phosphate." Journal of Toxicology and Environmental Health Sciences 1, no. 1 (2009): 001-007.
MLA Trupti D. Chaudhari, Susan Eapen and M. H. Fulekar. "Characterization of industrial waste and identification of potential micro-organism degrading tributyl phosphate." Journal of Toxicology and Environmental Health Sciences 1.1 (2009): 001-007.
   
DOI
URL http://academicjournals.org/journal/JTEHS/article-abstract/BE64F7B754

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