Journal of Toxicology and Environmental Health Sciences
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Article Number - F26537547667


Vol.6(8), pp. 147-153 , October 2014
DOI: 10.5897/JTEHS2014.0313
ISSN: 2006-9820



Full Length Research Paper

Determination of the effects of some artificial sweeteners on human peripheral lymphocytes using the comet assay



Zeynep Findikli
  • Zeynep Findikli
  • Department of Biology, Faculty of Arts and Science, Cumhuriyet University, Sivas, Turkey.
  • Google Scholar
Sifa Turkoglu
  • Sifa Turkoglu
  • Department of Biology, Faculty of Arts and Science, Cumhuriyet University, Sivas, Turkey.
  • Google Scholar







 Received: 02 August 2014  Accepted: 11 September 2014  Published: 31 October 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


In this study, the genotoxicity of the artificial sweeteners acesulfame potassium, aspartame, saccharin, and sorbitol, which are used in food industry and by patients with diabetes, was investigated in human peripheral lymphocyte cells using the single-cell gel electrophoresis (comet) technique. Human lymphocyte cells were treated with the substances for three hour at each of the three dosages (1.25, 2.5, and 5 ppm). The chemical additives were studied, and the related DNA damages in the study group were compared to the control group for each of the treatment dosages. The DNA breakages observed in the comet assay were assessed in terms of tail moment and tail DNA percent using the comet parameters. The statistical and photographic analyses were performed using SPSS 15 and BAB BS 200 Pro software, respectively. Based on the results for the short-term in vitro treatments, the 4 different food flavorings were found to have genotoxic effects.

 

Key words: Comet assay, DNA damages, artificial sweeteners, human peripheral lymphocytes.

Abbreviation:

ADI, Acceptable Daily Intake; FDA,  Food and Drug Administration; JECFA, Joint FAO/WHO Expert Committee on Food Additives (JECFA)


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APA Findikli, Z., & Turkoglu, S. (2014). Determination of the effects of some artificial sweeteners on human peripheral lymphocytes using the comet assay. Journal of Toxicology and Environmental Health Sciences, 6(8), 147-153.
Chicago Zeynep Fındıklı and Şifa Türkoğlu. "Determination of the effects of some artificial sweeteners on human peripheral lymphocytes using the comet assay." Journal of Toxicology and Environmental Health Sciences 6, no. 8 (2014): 147-153.
MLA Zeynep Fındıklı and Şifa Türkoğlu. "Determination of the effects of some artificial sweeteners on human peripheral lymphocytes using the comet assay." Journal of Toxicology and Environmental Health Sciences 6.8 (2014): 147-153.
   
DOI 10.5897/JTEHS2014.0313
URL http://academicjournals.org/journal/JTEHS/article-abstract/F26537547667

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