Journal of Veterinary Medicine and Animal Health
Subscribe to JVMAH
Full Name*
Email Address*

Article Number - 2B959F566284


Vol.9(11), pp. 290-299 , November 2017
https://doi.org/10.5897/JVMAH2017.0627
ISSN: 2141-2529


 Total Views: 0
 Downloaded: 0

Full Length Research Paper

Effect of a low dose of BCG-Phipps vaccine on the development of reactivity to tuberculin skin test in neonatal calves and adult cows



Lizzet Corona-Gómez
  • Lizzet Corona-Gómez
  • Departamento de Inmunología, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, INIFAP, Toluca, Mexico.
  • Google Scholar
Laura Jaramillo-Meza
  • Laura Jaramillo-Meza
  • Departamento de Inmunología, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, INIFAP, Toluca, Mexico.
  • Google Scholar
Rafael Pérez-González
  • Rafael Pérez-González
  • Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autonoma de México, México City, Mexico.
  • Google Scholar
Fernando Diosdado-Vargas
  • Fernando Diosdado-Vargas
  • Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autonoma de México, México City, Mexico.
  • Google Scholar
Julio Santiago-Cru
  • Julio Santiago-Cru
  • Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autonoma de México, México City, Mexico.
  • Google Scholar
Clara I Espitia-Pinzón
  • Clara I Espitia-Pinzón
  • Instituto de Investigaciones Biomédicas, Universidad Nacional Autonoma de México, México City, Mexico.
  • Google Scholar
Ricardo Lascurain
  • Ricardo Lascurain
  • Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autonoma de México, México City, Mexico.
  • Google Scholar
Fernando Díaz-Otero
  • Fernando Díaz-Otero
  • Departamento de Inmunología, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, INIFAP, Toluca, Mexico.
  • Google Scholar







 Received: 21 July 2017  Accepted: 12 September 2017  Published: 30 November 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Bovine tuberculosis (bTB) has a direct impact in the productive and reproductive efficiency of dairy cattle. Nowadays, disease control programs based on tuberculin testing and removal of infected cattle are unaffordable for the developing countries, since there is no program of financial compensation, especially in high bTB-prevalence herds. Thus, control strategies based on vaccination are considered the best alternative. The aim of this study was to evaluate whether a low dose of BCG-Phipps vaccine induces reactivity to tuberculin skin test and its duration in neonatal calves and adult cows. For the analysis of the former, 69 TB-free Holstein-Friesian calves less than one-month old were used; of which, 54 calves were subcutaneously inoculated with 104 CFU of the BCG-Phipps vaccine, while the rest remained without vaccination. Under similar conditions of immunization, 133 single intradermal comparative cervical (SICCT) reactors and 133 non-reactors Holstein-Friesian cows of different age were also analyzed. In calves, the SICCT-reactivity was evaluated periodically in the first 14 months post-vaccination (mpv) while, in adult cows, the effect of vaccination on the test was evaluated at six months post-vaccination. A comparative ELISA was used by measuring the antibody levels in the groups. In calves, reactivity frequencies of 7.4 and 3.7% at 3 and 5 mpv, respectively were recorded. This reactivity disappeared at six months. None calves in the control group were reactor during the study. There were no variations in the degree of reactivity in the group adult reactor cows. However, in the non-reactor group, a conversion of 12.8% at six mpv was recorded. In addition, the conversion percentage was higher in older cows than in younger cows (p<0.05). The specific antibody levels did not increase in the vaccinated groups. Data indicate that the low dose of BCG-Phipps vaccine used had a reduced effect on the development of a delayed type hypersensitivity to tuberculin in neonatal calves, and heifers lesser than one-year-old.

Key words: Bacillus Calmette-Guérin, bovine tuberculosis, M. bovis, single intradermal comparative tuberculin test.

Abdallah AM, Hill-Cawthorne GA, Otto TD, Coll F, Guerra-Assunção JA, Gao G, Naeem R, Ansari H, Malas TB, Adroub SA, Verboom T, Ummels R, Zhang H, Panigrahi AK, McNerney R, Brosch R, Clark TG, Behr MA, Bitter W, Pain A (2015). Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations. Sci. Rep. 5:15443.
Crossref

 

Bernardelli A (2007). Producción y control de tuberculina bovina y aviar. Derivado Proteico Purificado (DPP). Servicio Nacional de Sanidad y Calidad Agroalimentaria. Buenos Aires. Argentina. 

 
 

Bradford MMA (1976). Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
Crossref

 
 

Buddle BM, Hewinson RG, Vordermeier HM, Wedlock DN (2013). Subcutaneous administration of a 10-fold-lower dose of a commercial human tuberculosis vaccine, Mycobacterium bovis Bacillus Calmette-Guérin Danish, induced levels of protection against bovine tuberculosis and responses in the tuberculin intradermal test similar to those induced by a standard cattle dose. Clin. Vaccine Immunol. 20(10):1559-1562.
Crossref

 
 

Buddle BM, Shu D, Parlane NA, Subharat S, Heiser A, Hewinson RG, Vordermeier HM, Wedlock DN (2016). Vaccination of cattle with a high dose of BCG vaccine 3 weeks after experimental infection with Mycobacterium bovis increased the inflammatory response, but not tuberculous pathology. Tuberculosis (Edinb). 99:120-127.
Crossref

 
 

Buddle BM, Wedlock DN, Parlane NA, Corner LAL, de Lisle GW, Skinner MA (2003). Revaccination of neonatal calves with Mycobacterium bovis BCG reduced the level of protection against tuberculosis induced by a single vaccination. Infect. Immun. 71(11):6411-6419.
Crossref

 
 

Cardona PJ (2006). RUTI: A new chance to shorten the treatment of latent tuberculosis infection. Tuberculosis (Edinb). 86(3):273-289.
Crossref

 
 

Castillo-Rodal AI, Casta-ón-Arreola M, Hernández-Pando R, Calva JJ, Sada-Díaz E, López-Vidal Y (2006). Mycobacterium bovis BCG substrains confer different levels of protection against Mycobacterium tuberculosis infection in a BALB/c model of progressive pulmonary tuberculosis. Infect. Immun. 74(3):1718-1724.
Crossref

 
 

Cockle PJ, Gordon SV, Lalvani A, Buddle B.M, Hewinson RG, Vordenmeier HM (2002). Identification of novel Mycobacterium tuberculosis antigens with potential as diagnostic reagents or subunit vaccine candidates by comparative genomic. Infect. Immun. 70:6996-7003.
Crossref

 
 

Cousins DV, Wilton SD, Francis BR, Gow BL (1992).Use of polymerase chain reaction for rapid diagnosis of tuberculosis. J. Clin. Microbiol. 30(1):255-258.

 
 

Da Costa AC, Nogueira SV, Kipnis A, Junqueira-Kipnis AP (2014). Recombinant BCG: innovations on an old vaccine. Scope of BCG strains and strategies to improve long-lasting memory. Front. Immunol. 5:152.
Crossref

 
 

El-Sayed A, El-Shannat S, Kamel M, Casta-eda-Vazquez MA, Casta-eda-Vazquez H (2016). Molecular epidemiology of Mycobacterium bovis in humans and cattle. Zoonoses Public Health. 63(4):251-264.
Crossref

 
 

Estrada-Chavez C, Mancilla R, Arriaga-Díaz C, Pérez-González R, Díaz-Otero F (2001). Determination of anti-PPD antibodies in dairy herds with different prevalence of bovine tuberculosis in Mexico. Vet. Méx. 32:207-211.

 
 

González-González XE, Jaramillo-Meza L, Lascurain-Ledezma R, Torres-Barranca JI, Quevillon CE, Díaz-Otero F (2012). Evaluation of T lymphocyte subpopulations in cattle vaccinated against bovine tuberculosis: longitudinal comparative study. Rev. Mex. Cienc. Pecu. 3(2):137-154.

 
 

González-Salazar D, Díaz-Otero F, Jaramillo-Meza L, Pérez-González R, Padilla-Urbina J, Santillán-Flores MA, Arriaga-Díaz C, Bojórquez-Narváez L (2007). Evaluación de diferentes inmunógenos contra la tuberculosis bovina mediante la presencia de lesiones a la necropsia. Vet. Méx. 38(3):271-284.

 
 

Good M, Duignan A (2011). Perspectives on the history of bovine TB and the role of tuberculin in bovine TB eradication. Vet. Med. Int. 2011:410470.
Crossref

 
 

Gupta MP, Kumar H, Singla LD (2009) Trypanosomosis concurrent to tuberculosis in black bucks. Indian Vet. J. 86:727-728.

 
 

Hope JC, Thom ML, McAulay M, Mead E, Vordermeier HM, Clifford D, Hewinson RG, Villarreal-Ramos B (2011). Identification of surrogates and correlates of protection in protective immunity against Mycobacterium bovis infection induced in neonatal calves by vaccination with M. bovis BCG Pasteur and M. bovis BCG Danish. Clin. Vac. Immunol. 18(3):373-379.
Crossref

 
 

Hope JC, Thom ML, Villarreal-Ramos B, Vordermeier HM, Hewinson RG, Howard CJ (2005). Vaccination of neonatal calves with Mycobacterium bovis BCG induces protection against intranasal challenge with virulent M. bovis. Clin. Exp. Immunol. 139:48-56.
Crossref

 
 

Joung SM, Ryoo S (2013). BCG vaccine in Korea. Clin. Exp. Vaccine Res. 2(2):83-91.
Crossref

 
 

Kaufmann SHE, Lange C, Rao M, Balaji KN, Lotze M, Schito M, Zumla AI, Maeurer M (2014). Progress in tuberculosis vaccine development and host-directed therapies a state of the art review. Lancet Respir. Med. 2:301-320.
Crossref

 
 

Lienhardt C, Fruth U, Greco, M (2012). The blueprint for vaccine research and development: Walking the path for better TB vaccines. Tuberculosis (Edinb). 92(S1):33-35.
Crossref

 
 

Logan KE, Chambers MA, Hewinson RG, Hogarth PJ (2005). Frequency of IFN-γ producing cells correlates with adjuvant enhancement of bacilli Calmette-Guérin induced protection against Mycobacterium bovis. Vaccine 23(48-49):5526-5532.
Crossref

 
 

López-Valencia G, Rentería-Evangelista T, Williams JJ, Licea-Navarro A, Mora-Valle AL, Medina-Basurto G (2010). Field evaluation of the protective efficacy of Mycobacterium bovis BCG vaccine against bovine tuberculosis. Res. Vet. Sci. 88(1):44-49.
Crossref

 
 

Lyashchenko K, Whelan AO, Greenwald R, Pollock JM, Andersen P, Hewinson RG, Vordermeier HM (2004). Association of tuberculin-boosted antibody responses with pathology and cell-mediated immunity in cattle vaccinated with Mycobacterium bovis BCG and infected with M. bovis. Infect. Immun. 72(5):2462-2467.
Crossref

 
 

Norma Oficial Mexicana NOM-031-ZOO-1995. 27 de agosto 2008. Campa-a Nacional Contra la Tuberculosis Bovina (Mycobacterium bovis). Secretaria de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación. Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria. Avalable at: 

View

 
 

Office International des Epizooties (OIE) (2012). Manual of diagnostic tests and vaccines for terrestrial animal. Office International des Epizooties (OIE). Chapter 2.4.7. Bovine tuberculosis, pp. 674-689. Available at: 

View

 
 

Okkels LM, Brock I, Follmann F, Agger EM, Arend SM, T. H. M. Ottenhoff THM, Oftung F, Rosenkrands I, Andersen P (2003). PPE protein (Rv3873) from DNA segment RD1 of Mycobacterium tuberculosis: strong recognition of both specific T-cell epitopes and epitopes conserved within the PPE family. Infect. Immun. 71(11):6116-6123.
Crossref

 
 

Ritz N, Hanekom WA, Robins-Browne R, Britton WJ, Curtis N (2008). Influence of BCG vaccine strain on the immune response and protection against tuberculosis. FEMS Microbiol. Rev. 32(5):821-841.
Crossref

 
 

Schiller I, Oesch B, Vordermeier HM, Palmer MV, Harris BN, Orloski KA, Buddle BM, Thacker TC, Lyashchenko KP, Waters WR (2010). Bovine Tuberculosis: A review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication. Transbound. Emerg. Dis. 57(4):205-220.
Crossref

 
 

Siddiqui N, Price S, Hope J (2012). BCG vaccination of neonatal calves: potential roles for innate immune cells in the induction of protective immunity. Comp. Immunol. Microbiol. Infect. Dis. 35(3):219-226.
Crossref

 
 

Skinner MA, Wedlock DN, Buddie BM (2001). Vaccination of animals against Mycobacterium bovis. Rev. Sci. Technol. Off. Int. Epiz. 20(1):112-132.
Crossref

 
 

Thoen C, Lobue P, de Kantor I (2006). The important of Mycobacterium bovis as a zoonosis. Vet. Microbiol. 112(2-4):339-345.
Crossref

 
 

Thom ML, McAulay M, Vordermeier HM, Clifford D, Hewinson RG, Villarreal-Ramos B, Hope JC (2012). Duration of immunity against Mycobacterium bovis following neonatal vaccination with Bacillus Calmette-Guérin Danish: significant protection against infection at 12, but Not 24, Months. Clin. Vaccine Immunol. 19(8):1254-1260.
Crossref

 
 

Tree JA, Williams A, Clark S, Hall G, Marsh PD, Ivanyi J (2004). Intranasal bacilleCalmette–Guérin (BCG) vaccine dosage needs balancing between protection and lung pathology. Clin. Exp. Immunol. 138(3):405-409.
Crossref

 
 

Waters WR, Palmer MV, Buddle BM, Vordermeier HM (2012). Bovine tuberculosis vaccine research: Historical perspectives and recent advances. Vaccine 30:2611-2622.
Crossref

 
 

Wayne DW (2006). Bioestadística: base para el análisis de las ciencias de la salud. Fourth Ed., Limusa Editorial S. A. Mexico. ISBN: 978-968-18-6164-3

 
 

Whelan AO, Coad M, Upadhyay BL, Clifford DJ, Hewinson RG, Vordermeier HM (2011). Lack of correlation between BCG-induced tuberculin skin test sensitization and protective immunity in cattle. Vaccine 29(33):5453-5458.
Crossref

 
 

Zhang L, Ru HW, Chen FZ, Jin CY, Sun RF, Fan XY, Guo M, Mai JT, Xu WX, Lin QX, Liu J (2016). Variable virulence and efficacy of BCG vaccine strains in mice and correlation with genome polymorphisms. Mol. Ther. 24(2):398-405.
Crossref

 

 


APA Corona-Gómez, L., Jaramillo-Meza, L., Pérez-González, R., Diosdado-Vargas, F., Santiago-Cruz, J., Espitia-Pinzón, C. I., Lascurain, R., & Díaz-Otero, F. (2017). Effect of a low dose of BCG-Phipps vaccine on the development of reactivity to tuberculin skin test in neonatal calves and adult cows. Journal of Veterinary Medicine and Animal Health, 9(11), 290-299.
Chicago Lizzet Corona-G&omez, Laura Jaramillo-Meza, Rafael P&erez-Gonz&alez, Fernando Diosdado-Vargas, Julio Santiago-Cruz, , Clara I Espitia-Pinz&on, Ricardo Lascurain and Fernando D&iaz-Otero. "Effect of a low dose of BCG-Phipps vaccine on the development of reactivity to tuberculin skin test in neonatal calves and adult cows." Journal of Veterinary Medicine and Animal Health 9, no. 11 (2017): 290-299.
MLA Lizzet Corona-Goacute;mez, et al. "Effect of a low dose of BCG-Phipps vaccine on the development of reactivity to tuberculin skin test in neonatal calves and adult cows." Journal of Veterinary Medicine and Animal Health 9.11 (2017): 290-299.
   
DOI https://doi.org/10.5897/JVMAH2017.0627
URL http://academicjournals.org/journal/JVMAH/article-abstract/2B959F566284

Subscription Form