Journal of Yeast and Fungal Research
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Article Number - 290980061352


Vol.7(6), pp. 47-53 , November 2016
https://doi.org/10.5897/JYFR2016.0174
ISSN: 2141-2413


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Full Length Research Paper

Isolation, identification and characterization of yeast species from coffee waste collected from Sidama and Gedio zone



Birhanu Gizaw*
  • Birhanu Gizaw*
  • Microbial Biodiversity Directorate, Ethiopian Biodiversity institute, P. O. Box 30726 Addis Ababa, Ethiopia.
  • Google Scholar
Zerihun Tsegaye
  • Zerihun Tsegaye
  • Microbial Biodiversity Directorate, Ethiopian Biodiversity institute, P. O. Box 30726 Addis Ababa, Ethiopia.
  • Google Scholar
Genene Tefera
  • Genene Tefera
  • Microbial Biodiversity Directorate, Ethiopian Biodiversity institute, P. O. Box 30726 Addis Ababa, Ethiopia.
  • Google Scholar







 Received: 16 September 2016  Accepted: 20 October 2016  Published: 30 November 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Coffee waste represents the most abundant waste in Ethiopia, this study aims to isolate, identify and characterize yeast from coffee waste in order to utilize in the biotechnological process. 25 merged coffee waste samples were collected from Sidama and Gedio zone. Yeast strain was cultured on yeast peptone dextrose and Biolog universal yeast agar media. Pure yeast cells were suspended in sterile water at 49+ turbidity, 100 µ L and transferred into 96 wells of the biolog yeast micro Plate and incubated at 26°C for 24 to 72 h and read by the Micro Station Reader at a single wavelength of 590 nm, results were recorded and processed for identification by micro log3 software ver. 4.20.05. Biolog microstation  acceptable  result  ≥ 75% Probability and  ≥ 0.5 similarity index value identified 5 yeast species, Hanseiaspora valbyensis, (100%PROB,0.707SIM,), Hyphopichia burtonii A (98%PROB,0.060SIM), Rhodotorula hylophila (98%PROB, 0.060SIM), Rhodotrula aurantiaca A (100% PROB, 0.505SIM) and Pichia amenthionina var. menthionina, (PROB 96% SIM,0.714). There was no report on yeasts associated with coffee waste in Ethiopia for utilization in biotechnological process. Therefore characterization of yeasts is very important for industrial and environmental application.

Key words: Biolog, coffee, fermentation, micro station, Omni log, tetrazolium.

Adams MR, Dougan J (1981). Biological management of Coffee processing. Trop Sci. 123:178-196.

 

Aga E,BryngelssonT, Bekele E, Salomon B(2003). Genetic diversity of forest Arabica coffee (Coffee arabica) in Ethiopia as revealed by random amplified polymorphic DNA (RAPD) analysis. Hereditas138:36-46.

 

Agate AD, Bhat JV (1966). Role of pectinolytic yeasts in the degradation of mucilage layer of Coffea robusta cherries. Appl. Microbiol.14:256-260.

 

Alemayehu T, Esayas K, Kassu K (2007). Coffee development and marketing Improvement plan in Ethiopia. Proceeding of national workshop four decades of coffee research and development in Ethiopia, Addis Ababa. Pp. 375-387.

 

Amelia KK, Berhanu A, Suhaila OH, Marioni M (2010). Sustainable Utilization of Agro industrial waste through the Integration of Bioenergy and Mushroom Production. International Livestock Research Institute. 190 p.

 

Antier P, Minjares A, Roussos S, Raimbault M, Viniegragonzalez G (1993). Pectinase hyper Producing mutants of Aspergillus niger C28B25 for solid state fermentation of coffee pulp. J. Enzyme Microb.Technol. 15:1-1.
Crossref

 

Avallone S, Bernard G, Jean MB, Eugenia O, Joseph PG (2001). Microbiological and Biochemical Study of Coffee Fermentation. Curr. Microbiol. 42:252-256.
Crossref

 

Beech FW, Davenport RR (1970). The role of yeast in cider making. In: Rose, A.H., Harrison, I.S. (Eds.), Yeast. Academic Press. London. Pp. 73-146.

 

Biolog (1993). YT Microplate: Instruction for use. Biolog, Inc.

 

Boboye B, Dayo OL (2009). Comparative Evaluation of the Sensory Properties of Doughs Fermented with Yeasts Isolated from Orange. J. Biotechnol. 8:389-392.
Crossref

 

Bressani R (1979). Anti-physiological factors in coffee pulp. Coffee pulp: Composition, Technology and Utilization, IDRC Pub. 108E, Ottawa.

 

Demelo PGV, Soccol VT, Pandey A, Medeiros AB, Andrade LJM, Gollo AL, Soccol CR (2014). Isolation, Selection and evaluation of yeasts for use in fermentation of Coffee beans by the wet process. Int. J. Food Microbiol. 1(188):60-66.
Crossref

 

Fleet GH (2003).Yeast interactions and wine flavour. Int. J. Food Microbiol. 86(1-2):11-22.
Crossref

 

Frengova G, Simova DE, Beshkova DM (2003). Carotenoid production by lactose negative yeasts co-cultivated with lactic acid bacteria in whey ultrafiltrate. Z. Naturforsch. C 58(7-8):562-567.
Crossref

 

Gellért B, Christoph ME, Thomas HK (2000). Inter and IntraSpecific Differentiation of Natural Wine Strains of Hanseniaspora (Kloeckera) by Physiological and Molecular Methods. J. Food Technol. Biotechnol. 39(1):19-28.

 

Jorgensen H, Sanadi AR, Felby C, Lange N E K, Fischer M, Ernst S (2010). Production of ethanol and feed by high dry matter hydrolysis and fermentation of palm kernel presscake. J. Appl. Biochem. Biotechnol. 161:318-332.
Crossref

 

Katos SI, Murak M, Takeuchi M, Tokue CA (2007). Molecular cloning and characterization of an alpha amylase from Pichia burtoni. J. Biosci. Biotechnol. Biochem.12:3007-3013.
Crossref

 

Kurtzman CP, Robnett CJ (1998).Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4):331-371.
Crossref

 

Maragatham C, Panneerselvam A (2011). Production of Single Cell Protein from Yeast using Papaya Extract Medium. J. Adv. Appl. Sci. Res. 2(2):14-18.

 

Martinez CD (1989). Simple technology to cultivate Pleurotus on coffee pulp in the tropics. J. Mushroom Sci. 12(Part 2):169-178.

 

Masoud W, Lene B, Lene J, Mogens J (2004). Yeast involved infermentation of Coffee Arabica in East Africa determined by genotyping and by direct denaturating gradient gel electrophoresis. Yeast 21:549-556.
Crossref

 

Mesa L, González E, Cara C, Ruiz E, Castro E, Mussatt SI (2010). An approach to optimization of Enzymatic hydrolysis from sugarcane bagasse based on organo solvent pretreatment. J. Chem. Technol. Biotechnol. 85:1092-1098.
Crossref

 

Mussatto SI, Dragone G, Roberto IC (2006). Brewer's spent grain: generation, characteristics and Potential applications. J. Cereal Sci. 43:1-14.
Crossref

 

Mutua J (2000). Post-harvest handling and processing of coffee in African countries. Rome, Italy.

 

Noel HTG, Bernadette CM, Rosario GM (2014). Isolation, Screening and Characterization of Yeasts with Amyloytic, Lipolytic, and Proteolytic Activities from the Surface of Philippine Bananas (Musa spp.) Philippine. J. Sci.143 (1): 81-87

 

Pan C, Zhang S, Fan Y, Hou H(2010). Bioconversion of corncob to hydrogen using anaerobic mixed microflora. Int. J. Hydrogen Energy. 35:2663-2669.
Crossref

 

Phaff HJ (1970). Hanseniaspora Zikes. In. J. Lodder, (ed.), The Yeasts. North Holland Publ. Co., Amsterdam. Pp. 209-225.

 

Qureshi N, Ezeji TC (2008). Butanol "a superior biofuel" production from agricultural Residues (renewable biomass): recent progress in technology. Biofuel Bioprod. Bioref. 2:319-330.
Crossref

 

Rad MM, Zafarghandi AS, Zabihi MA, Tavallaee M, Mirdamadi Y (2012). Identification of Candida species Associated with vulvovaginal Candidiasis by Multiple PCR. Infect. Dis. Obstet. Gynecol. 1-5.230-240

 

Sheela SH, Ahmed MF, Gomes DJ (2008). Fuel Ethanol Production from Molasses by Some Indigenous Yeast Isolates. Bangladesh J. Microbiol. 25:129-133.

 

Silva CF, Batista LR, Abreu LM, Dias ES, Schwan RF (2008). Succession of bacterial and fungal communities during natural coffee (Coffea arabica) fermentation. J. Food Microbiol. 25:951-957.
Crossref

 

Silva JPA, Mussatto SI, Roberto IC(2010). The influence of initial xylose concentration, agitation and aeration of ethanol production by Pichia stipitis from ric straw hemi cellulos ichydrolysate. J. Appl. Biochem. Biotechnol.162:1306-1315.
Crossref

 

Takeuchi A, Shizumi IA, Nishiyama Y, Murak OS, Takue CA (2006). Putification and characterization of alpha amaylase of Pichia burutoniii isolated from the traditional starter Murcha in Nepa. Biosci. Biotechnol. Biochem. 70:3019-3024.
Crossref

 


APA Gizaw, B., Tsegaye, Z., & Tefera, G. (2016). Isolation, identification and characterization of yeast species from coffee waste collected from Sidama and Gedio zone. Journal of Yeast and Fungal Research , 7(6), 47-53.
Chicago Birhanu Gizaw, Zerihun Tsegaye and Genene Tefera. "Isolation, identification and characterization of yeast species from coffee waste collected from Sidama and Gedio zone." Journal of Yeast and Fungal Research 7, no. 6 (2016): 47-53.
MLA Birhanu Gizaw, Zerihun Tsegaye and Genene Tefera. "Isolation, identification and characterization of yeast species from coffee waste collected from Sidama and Gedio zone." Journal of Yeast and Fungal Research 7.6 (2016): 47-53.
   
DOI https://doi.org/10.5897/JYFR2016.0174
URL http://academicjournals.org/journal/JYFR/article-abstract/290980061352

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