Journal of Yeast and Fungal Research
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Article Number - 8E42DA761081


Vol.7(5), pp. 39-46 , October 2016
https://doi.org/10.5897/JYFR2016.0172
ISSN: 2141-2413


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Full Length Research Paper

Molecular identification of yeasts associated with raw cow milk from peri-urban farms in Kaduna State, Nigeria



Mbuk E. U.
  • Mbuk E. U.
  • Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
  • Google Scholar
Kwaga J. K. P.
  • Kwaga J. K. P.
  • Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
  • Google Scholar
Bale J. O. O.
  • Bale J. O. O.
  • National Animal Production Research Institute, Ahmadu Bello University, Shika, P.M.B. 1096, Zaria, Kaduna State, Nigeria.
  • Google Scholar
Umoh J. U.
  • Umoh J. U.
  • Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
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 Received: 26 August 2016  Accepted: 02 October 2016  Published: 31 October 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


This study aimed to examine the diversity of yeasts associated with bovine mastitis by employing molecular techniques in yeast identification. A total of 300 composite milk samples were collected from 26 peri-urban farms and examined. After culture, 37 (12.3%) fungal isolates were identified using the API 20C AUX. Polymerase chain reaction (PCR) and sequencing of the domains D1 and D2 of the 26S rRNA gene identified eleven (11) of these yeasts as Candida albicans (3 isolates), Saccharomyces cerevisiae (1 isolate) and Pichia Kudriavzevii (7 isolates). The D1/D2 26S rRNA gene sequences were 100% identical for the yeast isolates within the same species. It was found that all C. albicans had ability to produce germ tubes by incubation on human serum and chlamydospores. Antimycotic sensitivity showed that 78% fungal isolates were sensitive to amphotericin B. The presence of isolates of the pathogenic yeast, C. albicans in this study raises the possibility of cow milk being a vehicle of transmission of pathogenic yeasts.

Key words: Bovine, milk, mastitis, yeasts, molecular identification.

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APA Mbuk, E. U., Kwaga, J. K. P., Bale J. O. O., & Umoh, J. U. (2016). Molecular identification of yeasts associated with raw cow milk from peri-urban farms in Kaduna State, Nigeria. Journal of Yeast and Fungal Research , 7(5), 39-46.
Chicago Mbuk E. U., Kwaga J. K. P., Bale J. O. O. and Umoh J. U.. "Molecular identification of yeasts associated with raw cow milk from peri-urban farms in Kaduna State, Nigeria." Journal of Yeast and Fungal Research 7, no. 5 (2016): 39-46.
MLA Mbuk E. U., et al. "Molecular identification of yeasts associated with raw cow milk from peri-urban farms in Kaduna State, Nigeria." Journal of Yeast and Fungal Research 7.5 (2016): 39-46.
   
DOI https://doi.org/10.5897/JYFR2016.0172
URL http://academicjournals.org/journal/JYFR/article-abstract/8E42DA761081

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