Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 112

Full Length Research Paper

Molecular identification of yeasts associated with raw cow milk from peri-urban farms in Kaduna State, Nigeria

Mbuk E. U.
  • Mbuk E. U.
  • Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
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Kwaga J. K. P.
  • Kwaga J. K. P.
  • Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
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Bale J. O. O.
  • Bale J. O. O.
  • National Animal Production Research Institute, Ahmadu Bello University, Shika, P.M.B. 1096, Zaria, Kaduna State, Nigeria.
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Umoh J. U.
  • Umoh J. U.
  • Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
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  •  Received: 26 August 2016
  •  Accepted: 02 October 2016
  •  Published: 31 October 2016

Abstract

This study aimed to examine the diversity of yeasts associated with bovine mastitis by employing molecular techniques in yeast identification. A total of 300 composite milk samples were collected from 26 peri-urban farms and examined. After culture, 37 (12.3%) fungal isolates were identified using the API 20C AUX. Polymerase chain reaction (PCR) and sequencing of the domains D1 and D2 of the 26S rRNA gene identified eleven (11) of these yeasts as Candida albicans (3 isolates), Saccharomyces cerevisiae (1 isolate) and Pichia Kudriavzevii (7 isolates). The D1/D2 26S rRNA gene sequences were 100% identical for the yeast isolates within the same species. It was found that all C. albicans had ability to produce germ tubes by incubation on human serum and chlamydospores. Antimycotic sensitivity showed that 78% fungal isolates were sensitive to amphotericin B. The presence of isolates of the pathogenic yeast, C. albicans in this study raises the possibility of cow milk being a vehicle of transmission of pathogenic yeasts.

Key words: Bovine, milk, mastitis, yeasts, molecular identification.