Journal of Yeast and Fungal Research
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Article Number - E216C522268


Vol.1(1), pp. 015-024 , February 2010

ISSN: 2141-2413


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Full Length Research Paper

Study on combined antimicrobial activity of some biologically active constituents from wild Moringa peregrina Forssk.


M. A. Abdel-Rahman Tahany1*, A. K. Hegazy1, A. Mohsen Sayed1, H. F. Kabiel1, T. El-Alfy2 and S. M. El-Komy2




1Cairo University, Faculty of Science, Cairo, Egypt.

2Cairo University, Faculty of Pharmacy, Cairo, Egypt.


Email: [email protected]






 Accepted: 31 October 2009  Published: 28 February 2010

Copyright © 2010 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Three plants were used in this study: Moringa pregrina, Achillea fragrantissimaand Coleome droserifolia. Six active constituents were separated from n-hexane and ethyl acetate fractions of Moringa pregrina. These active constituents were lupeol acetate, α-amyrin, β-amyrin, β-sitosterol, β-sitosterol-3-O- β-D-glucoside and apignin were assayed individually and in combination against pathogenic bacteria and fungi. All constituents were proved to be more antibacterial than antifungal agents. Aspergillus flavus and Fusarium solani were completely resistant to all constituents. α-amyrin was the most effective antibacterial compound. The least relative activity was achieved by β-sitosterol against Bacillus subtilis compared to ampicillin. Reasonable antifungal activity was recorded in case of lupeol acetate, α-amyrin and β-amyrin, while β-sitosterol and β-sitosterol-3-O- β-D-glucoside, revealed no antifungal activity.  Apignin missed both antifungal and antibacterial activities.  Low MICs were detected by α-amyrin, β-amyrin and β-sitosterol-3-O-β-D-glucoside against all tested bacteria. Concerning fungi, β-sitosterol and β-sitosterol-3-O-β-D-glucoside showed no antimycotic activity. Lupeol acetate, α-amyrin and β-amyrin, however, have slightly high MICs for all tested dermatophytic fungi compared to that of fluconazole. Among ninety nine assayed combination mixtures, thirty seven synergistic combination mixtures were detected which exerts 37 synergisms against different pathogens with FICI less than 0.5, which indicates high efficacy of combination mixtures over monotherapy treatments.

 

Key words: Combined antimicrobial therapy, active constituents, Moringa peregrina, Achillea fragrantissimaColeome droserifolia.


APA (2010). Study on combined antimicrobial activity of some biologically active constituents from wild Moringa peregrina Forssk.. Journal of Yeast and Fungal Research , 1(1), 015-024.
Chicago M. A. Abdel-Rahman Tahany, A. K. Hegazy, A. Mohsen Sayed, H. F. Kabiel, T. El-Alfy and S. M. El-Komy. "Study on combined antimicrobial activity of some biologically active constituents from wild Moringa peregrina Forssk.." Journal of Yeast and Fungal Research 1, no. 1 (2010): 015-024.
MLA M. A. Abdel-Rahman Tahany, et al. "Study on combined antimicrobial activity of some biologically active constituents from wild Moringa peregrina Forssk.." Journal of Yeast and Fungal Research 1.1 (2010): 015-024.
   
DOI https://doi.org/
URL http://academicjournals.org/journal/JYFR/article-abstract/E216C522268

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