Research in Pharmaceutical Biotechnology
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Article Number - 24CC1CE49464


Vol.5(2), pp. 13-21 , December 2014
https://doi.org/10.5897/RPB13.0095
ISSN: 2141-2324


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Full Length Research Paper

Antibacterial activity of alkaloidal compound isolated from leaves of Catharanthus roseaus (L.) against multi-drug resistant strains



Mohammed A. Abd Ali*
  • Mohammed A. Abd Ali*
  • Biology Department, College of Science, Missan University, Iraq.
  • Google Scholar
Abdulla H. Lafta
  • Abdulla H. Lafta
  • Biology Department, College of Science, Al basrah University, Iraq
  • Google Scholar
Sami K.H. Jabar
  • Sami K.H. Jabar
  • College of Dentistry, Missan University, Iraq.
  • Google Scholar







 Received: 16 October 2014  Accepted: 18 December 2014  Published: 31 December 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The following alkaloids were isolated from Catharanthus roseus L. leaves from the yellow crystal as white powder at (38°C). The chemical and physical properties were studied by thin layer chromatography (TLC), IR-spectrum (IR), Ultraviolet - visible spectrum (UV) and melting point (mp). Standard strains of bacteria: Staphylococcus aureus NCTC 6571, Escherichia coli NCTC 5933 and the clinical multidrug resistance (MDR) S. aureus, E. coli and P. aeruginosa were tested with the alkaloids. The Catharanthus ruseus (L.) alkaloids inhibited both type of bacteria strains of gram positive strains S. aureus NCTC 6571,*S. aureus and *E. coli as gram negative strains when concentrations increase (125, 250, 500 mg/ml) with C. ruseus L. alkaloids being the most active in (500 mg/ml) concentrations against gram positive S. aureus bacteria than gram negative bacteria E. coli, while the clinical resistance *P. aeruginosa showed resistance against C. ruseus L. alkaloids in all concentrations (500, 125, 250 mg/ml). The minimum inhibitory concentration (MIC) (µg/ml) of the alkaloidal compound were also determined. Finally, a test was also carried out to examine the cytotoxicity assay methods towards human red blood cells have no cyto-toxicity in all concentration. The good antimicrobial potency of the alkaloidal compound of C. ruseus L. indicates the treatment of (MDR) as an alternative to the costly antibiotics.

 

Key words: Catharanthus roseus L. leaves, thin layer chromatography (TLC), alkaloid compound, antibacterial activity. 

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APA Ali, M. A. A., Lafta, A. H., &. Jabar, S. K. H. (2014). Antibacterial activity of alkaloidal compound isolated from leaves of Catharanthus roseaus (L.) against multi-drug resistant strains. Research in Pharmaceutical Biotechnology, 5(2), 13-21.
Chicago Mohammed A. Abd Ali, Abdulla H. Lafta and Sami K.H. Jabar. "Antibacterial activity of alkaloidal compound isolated from leaves of Catharanthus roseaus (L.) against multi-drug resistant strains." Research in Pharmaceutical Biotechnology 5, no. 2 (2014): 13-21.
MLA Mohammed A. Abd Ali, Abdulla H. Lafta and Sami K.H. Jabar. "Antibacterial activity of alkaloidal compound isolated from leaves of Catharanthus roseaus (L.) against multi-drug resistant strains." Research in Pharmaceutical Biotechnology 5.2 (2014): 13-21.
   
DOI https://doi.org/10.5897/RPB13.0095
URL http://academicjournals.org/journal/RPB/article-abstract/24CC1CE49464

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