Research in Pharmaceutical Biotechnology

  • Abbreviation: Res. Pharm. Biotech.
  • Language: English
  • ISSN: 2141-2324
  • DOI: 10.5897/RPB
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

Antioxidant activity of n-butanol extract of celery (Apium graveolens) seed in streptozotocin-induced diabetic male rats

Jabbar A. A. Al-Sa'aidi
  • Jabbar A. A. Al-Sa'aidi
  • Physiology and Pharmacology Department, College of Veterinary Medicine, Al-Qadisiya University, Iraq.
  • Google Scholar
Mohsen N. A. Alrodhan
  • Mohsen N. A. Alrodhan
  • Deptartment of Internal and Preventive Medicine, College of Veterinary Medicine, Al-Qadisiya University, Iraq.
  • Google Scholar
Ahmed K. Ismael
  • Ahmed K. Ismael
  • Physiology and Pharmacology Department, College of Veterinary Medicine, Al-Qadisiya University, Iraq.
  • Google Scholar


  •  Accepted: 20 November 2012
  •  Published: 30 November 2012

Abstract

 

The potent of n-butanol extract of celery (Apium graveolens) seed in ameliorating the lipid peroxidation and antioxidant status was investigated in streptozotocin-induced diabetic rats. Thirty two mature male rats were assigned to four groups, non-diabetic control and three diabetic groups. Diabetes was induced by single injection with streptozotocin (60 mg/kg b.w., i.p.). Rats ≥ 200 mg/dl of blood glucose were used as diabetic. Diabetic groups (D, B, and I) were drenched with drinking water, n-butanol extract (60 mg/kg, b.w.), or injected with insulin (4 IU/animal), respectively for 21 days. On day 22, body weight gain was registered and male rats were sacrificed. Blood and liver subcellular fluid was obtained to assess blood glucose level and subcellular activity of Alanine aminotranferease (ALT), Aspartate aminotransferase (AST), catalase, Super oxide dismutase (SOD), Glutathione (GSH)-transferase and -reductase, and assessment of Malondialdehyde (MDA) and glutathione concentrations. Diabetic rats (D) showed marked increased blood glucose, decreased weight gain, increased activity of ALT, SOD, CAT, GSH-transferase, decreased GSH-reductase and normal AST. N-butanol extract of celery seed (B) or insulin (I) therapy moderated blood glucose within normal range, enhanced body weight gain and normalized the activities of all antioxidant enzymes. In conclusion, n-butanol extract of celery seed have potent role in ameliorating stressful complications accompanied by diabetes mellitus.

 

Key words: Celery, Apium graveolens, diabetes mellitus, antioxidants.