Research in Pharmaceutical Biotechnology
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Article Number - 264C36F498


Vol.4(2), pp. 24-29 , November 2012
https://doi.org/10.5897/RPB12.002
ISSN: 2141-2324


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Full Length Research Paper

Antioxidant activity of n-butanol extract of celery (Apium graveolens) seed in streptozotocin-induced diabetic male rats



Jabbar A. A. Al-Sa'aidi
  • Jabbar A. A. Al-Sa'aidi
  • Physiology and Pharmacology Department, College of Veterinary Medicine, Al-Qadisiya University, Iraq.
  • Google Scholar
Mohsen N. A. Alrodhan
  • Mohsen N. A. Alrodhan
  • Deptartment of Internal and Preventive Medicine, College of Veterinary Medicine, Al-Qadisiya University, Iraq.
  • Google Scholar
Ahmed K. Ismael
  • Ahmed K. Ismael
  • Physiology and Pharmacology Department, College of Veterinary Medicine, Al-Qadisiya University, Iraq.
  • Google Scholar







 Accepted: 20 November 2012  Published: 30 November 2012

Copyright © 2012 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


 

The potent of n-butanol extract of celery (Apium graveolens) seed in ameliorating the lipid peroxidation and antioxidant status was investigated in streptozotocin-induced diabetic rats. Thirty two mature male rats were assigned to four groups, non-diabetic control and three diabetic groups. Diabetes was induced by single injection with streptozotocin (60 mg/kg b.w., i.p.). Rats ≥ 200 mg/dl of blood glucose were used as diabetic. Diabetic groups (D, B, and I) were drenched with drinking water, n-butanol extract (60 mg/kg, b.w.), or injected with insulin (4 IU/animal), respectively for 21 days. On day 22, body weight gain was registered and male rats were sacrificed. Blood and liver subcellular fluid was obtained to assess blood glucose level and subcellular activity of Alanine aminotranferease (ALT), Aspartate aminotransferase (AST), catalase, Super oxide dismutase (SOD), Glutathione (GSH)-transferase and -reductase, and assessment of Malondialdehyde (MDA) and glutathione concentrations. Diabetic rats (D) showed marked increased blood glucose, decreased weight gain, increased activity of ALT, SOD, CAT, GSH-transferase, decreased GSH-reductase and normal AST. N-butanol extract of celery seed (B) or insulin (I) therapy moderated blood glucose within normal range, enhanced body weight gain and normalized the activities of all antioxidant enzymes. In conclusion, n-butanol extract of celery seed have potent role in ameliorating stressful complications accompanied by diabetes mellitus.

 

Key words: Celery, Apium graveolens, diabetes mellitus, antioxidants.


APA (2012). Antioxidant activity of n-butanol extract of celery (Apium graveolens) seed in streptozotocin-induced diabetic male rats. Research in Pharmaceutical Biotechnology, 4(2), 24-29.
Chicago Jabbar A. A. Al-Sa&#;aidi, Mohsen N. A. Alrodhan and Ahmed K. Ismael. "Antioxidant activity of n-butanol extract of celery (Apium graveolens) seed in streptozotocin-induced diabetic male rats." Research in Pharmaceutical Biotechnology 4, no. 2 (2012): 24-29.
MLA Jabbar A. A. Al-Sa&#;aidi, Mohsen N. A. Alrodhan and Ahmed K. Ismael. "Antioxidant activity of n-butanol extract of celery (Apium graveolens) seed in streptozotocin-induced diabetic male rats." Research in Pharmaceutical Biotechnology 4.2 (2012): 24-29.
   
DOI https://doi.org/10.5897/RPB12.002
URL http://academicjournals.org/journal/RPB/article-abstract/264C36F498

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