Research in Pharmaceutical Biotechnology

  • Abbreviation: Res. Pharm. Biotech.
  • Language: English
  • ISSN: 2141-2324
  • DOI: 10.5897/RPB
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

Cinnamomum zeylanicum extract inhibits proinflammatory cytokine TNFµ: in vitro and in vivo studies

Kalpana Joshi
  • Kalpana Joshi
  • Department of Biotechnology, Sinhgad College of Engg, Pune, India-411041.
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Shyam Awte
  • Shyam Awte
  • Poona College of Pharmacy, Pune 411029, India-411029
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Payal Bhatnagar
  • Payal Bhatnagar
  • Poona College of Pharmacy, Pune 411029, India-411029
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Sameer Walunj
  • Sameer Walunj
  • Division of Biochemistry, Department of Chemistry, University of Pune, Pune, India 411007.
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Rajesh Gupta
  • Rajesh Gupta
  • Division of Biochemistry, Department of Chemistry, University of Pune, Pune, India 411007.
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Swati Joshi
  • Swati Joshi
  • National Chemical Laboratory (NCL), Pune, India- 411007.
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Sushma Sabharwal
  • Sushma Sabharwal
  • Division of Biochemistry, Department of Chemistry, University of Pune, Pune, India 411007.
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Sarang Bani
  • Sarang Bani
  • Indian Institute of Integrative Medicine, Canal Road, Jammu-180 001, India
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A. S. Padalkar
  • A. S. Padalkar
  • Department of Biotechnology, Sinhgad College of Engg, Pune, India-411041.
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  • Article Number - 4901513455
  • Vol.2(2), pp. 014-021, April 2010
  •  Accepted: 30 December 2009
  •  Published: 30 April 2010

Abstract

 

Cinnamomum zeylanicum, a commonly used spice and well-known medicinal plant has been reported to have anti-diabetic, anti-oxidant and anti-microbial properties. We have investigated its anti-inflammatory activity using ethanol extract obtained from bark. In vitro and in vivo experiments were performed targeting TNF-α using flow cytometry. Ethanol extract of C. zeylanicum showed suppression of intracellular release of TNF-α in murine neutrophils as well as leukocytes in pleural fluid. The extract was found to inhibit TNF-α gene expression in LPS-stimulated human PBMCs at 20 µg/ml concentration. A potent anti-inflammatory activity of Cinnamon extract is suggestive of its anti-arthritic activity, which could be confirmed in various models of arthritis.

 

Key words: Cinnamomum zeylanicum, TNF-α, flow cytometry, anti-inflammatory, gene expression.