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Article Number - 56BA97955013


Vol.6(1), pp. 1-7 , August 2015
https://doi.org/10.5897/RPB2015. 0101
ISSN: 2141-2324


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Full Length Research Paper

Protective effect of HAMI 3379 against high glucose-induced PC12 cell injury



Chunzhen Zhao
  • Chunzhen Zhao
  • Department of Pharmacology and Applied Pharmacology Laboratory, Weifang Medical University, Weifang, China.
  • Google Scholar
Lin Wang
  • Lin Wang
  • Department of Pharmacology and Applied Pharmacology Laboratory, Weifang Medical University, Weifang, China.
  • Google Scholar
Wanzhong Li*
  • Wanzhong Li*
  • Department of Pharmaceutics and Applied Pharmacology Laboratory, Weifang Medical University, Weifang, China.
  • Google Scholar







 Received: 04 August 2015  Accepted: 12 August 2015  Published: 31 August 2015

Copyright © 2015 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Diabetic neuropathy is one of the most common diabetic complications, associated with long time exposure to high glucose. Hyperglycemia induces the production of reactive oxygen species (ROS) and reactive nitrogen species contributing to neuronal damage. We have previously reported that HAMI 3379, a selective antagonist of CysLT2 receptor, is involved in neuron injury after ischemia. In this study, we investigated the protective effect of HAMI 3379 against high glucose-induced cell injury in PC12 neural cells. The PC12 cells were pretreated with various concentrations of HAMI 3379 (0.001 ~ 10 µM) for 1 h and then co-treated with HAMI 3379 and D-glucose (100 mM) for 48 h. HAMI 3379 (0.001 ~ 10 µM) protected PC12 cells against high glucose toxicity, as determined by cell viability and apoptosis after the evaluation by Hoechst 33342 staining assay. In addition, the increased nitric oxide production and nitric oxide synthase (NOS) activity in the media induced by high glucose was significantly inhibited by HAMI 3379. These results demonstrate that HAMI 3379 protected PC12 cells against high glucose-induced neurotoxicity by inhibition of apoptosis, nitric oxide production and nitric oxide synthase activity, suggesting that CysLT2 receptor may be a potential target for diabetic neuropathy, and its antagonist may play a crucial role in the treatment of diabetic neuropathy.

 

Key words: Cysteinyl leukotriene receptor, glucose, nitric oxide synthase, nitric oxide.

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APA Zhao, C., Wang, L., & Li*, W. (2015). Protective effect of HAMI 3379 against high glucose-induced PC12 cell injury. Research in Pharmaceutical Biotechnology, 6(1), 1-7.
Chicago Chunzhen Zhao, Lin Wang and Wanzhong Li. "Protective effect of HAMI 3379 against high glucose-induced PC12 cell injury." Research in Pharmaceutical Biotechnology 6, no. 1 (2015): 1-7.
MLA Chunzhen Zhao, Lin Wang and Wanzhong Li. "Protective effect of HAMI 3379 against high glucose-induced PC12 cell injury." Research in Pharmaceutical Biotechnology 6.1 (2015): 1-7.
   
DOI https://doi.org/10.5897/RPB2015. 0101
URL http://academicjournals.org/journal/RPB/article-abstract/56BA97955013

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