A microbial fuel cell (MFC) reactor with two compartments has been constructed from cheap materials and used for electricity generation from artificial mineral glucose medium (MGM) and test the adding or not adding the mediators during four separate experiment. The maximum voltages yield at external resistance 30 Ω on the mediator - less experiments was 401 mV, while when the mediators have been methylene blue, neutral red and potassium ferricyanide the maximum voltages yield were , 684, 482 and 523.6, respectively. The Coulombic efficiency in the mediator - less experiments 16.17%, while 19.74, 18.72 and 13.64%, respectively in the mediators experiments. Thereby it has proved that adding methylene blue as mediator for electrons shuttling optimized the voltage yield more than mediator - less by 70.57%, as well as more than the other tested mediators.
Key words: Electricity generation, mediator, mediator - less, microbial fuel cell, two - compartment.
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