Isolation and screening for amylase producing Penicillium species from soil samples
June 2025
Several microorganisms can degrade amylose (starch), but only a few produce substantial amounts of enzymes that can completely hydrolyze it. Fungi are the primary producers of amylase. This study aimed to screen Penicillium species isolated from soil at various sites within the University of Benin for their amylase production. Penicillium spp. were isolated by using serial dilution and pour plate methodology, then...
Assessment of glycemic control and its determinants in diabetic patients at the National Reference University Hospital Center (CHU-RN) of N'Djamena
June 2025
Diabetes is a significant public health problem, and its prevalence is increasing sharply. Preventing complications requires adequate control of blood sugar levels. The objective of this study was to monitor biological markers for the comprehensive management of diabetics. Blood glucose and HbA1c measurements were performed using biochemical analyzers (PC400 and HemoCue HbA1c 501). A total of 115 patients were included,...
Assessment of the genetic diversity in putative cowpea mutants using hierarchical clustering and principal component analysis
June 2025
To provide insights for optimizing breeding programmes, including parental selection and hybridization strategies, the genetic diversity of 11 putative cowpea mutants was evaluated across two ecological zones in Ghana using hierarchical clustering and Principal Component Analysis (PCA). The objectives were to assess the extent of genetic diversity and identify distinct genetic groups, to identify how different...
Drug resistance profiles and genetic diversity of Mycobacterium tuberculosis complex isolates from pulmonary tuberculosis patients co-infected with HIV and Helminths at Jamot Hospital in Yaoundé, Cameroon
May 2025
Tuberculosis (TB) remains a substantial public health problem due to co-infections, drug resistance, and the genetic diversity of Mycobacterium tuberculosis complex (MTBC). This study sought to evaluate drug resistance and genetic diversity of MTBC isolates from pulmonary TB patients co-infected with HIV and helminths at Jamot Hospital in Yaoundé, Cameroon. A cross-sectional design included 263 smear-positive...
Genetic diversity of isolates of swollen shoot-badnavirus detected in tolerant and sensitive cocoa trees (Theobroma cacao L.) in Côte d'Ivoire
May 2025
Cocoa swollen shoot virus disease (CSSD-badnavirus) is one of the major diseases responsible for the destruction of cocoa plantations. The objective of this study was to determine the genetic diversity of swollen shoot viruses detected in potentially tolerant and sensitive cocoa trees. Scions taken from 320 cocoa accessions established in seven CSSD-badnavirus-infected regions of Côte d'Ivoire were grafted...
Co-infections HIV-HBV and COVID-19: Frequencies, toxicity (liver and kidney) in pulmonary tuberculosis patients at Jamot Hospital in Yaoundé, Cameroon
May 2025
Liver and kidney toxicities are major concerns in tuberculosis (TB) patients due to drug treatments. This study investigated the frequencies and toxicities of HIV, Hepatitis B Virus (HBV), and COVID-19 co-infections in pulmonary TB patients. A prospective cohort study (September 2022-December 2023) at Jamot Hospital, Yaoundé, included 300 TB patients with sputum, blood, and nasopharyngeal samples collected....
Biodiversity and food security in Mali: The role of molecular surveillance in the detection of GMOS
May 2025
Mali though rich in agricultural resources, imports a significant portion of its food to meet the needs of its growing population. These foods, sourced from various countries, may include genetically modified organisms (GMOs), whose distribution is subject to strict regulations. The Cartagena Protocol, ratified by Mali, mandates rigorous monitoring of GMO circulation to avoid risks to biodiversity and human health. In...
Optimization of the co-production of biomethane from cashew apple pulp and cow dung in a mesophilic regime
April 2025
Anaerobic digestion is a technology based on the degradation of organic matter by microorganisms in an anaerobic environment. Anaerobic co-digestion involves mixing two or more methanizable substrates to optimize methane production ratios. This study focuses on the bioenergy recovery of the biodegradable fraction of waste from the Casamance region. It consists of evaluating the quantitative and qualitative production of...
Single nucleotide polymorphisms (SNPs) and their genotypes in three breeds of sheep
April 2025
Single nucleotide polymorphisms (SNPs) and their genotypes in the three breeds of sheep were conducted with ninety (90) sheep, thirty from each breed (30 Balami, 30 Yankasa, and 30 Uda) sheep were used for the study. Phenotypic characterization, blood sample collection, DNA extraction, polymerase chain reaction (PCR) and Single-Strand Conformational Polymorphism Analysis (SSCPA), agarose gel electrophoresis, sequencing...
Heavy metals and vitamin content of “Kunun-Zaki” drink, pulp and their freeze-dried forms
April 2025
Significant adverse health effects of certain heavy metals were reported in the dried samples of food. This study was aimed at evaluating vitamins and heavy metals content of unsweetened/sweetened “kunun-zaki” drink/pulp, before/after lyophilizing. The materials tested were unsweetened and sweetened “kunun-zaki” drink-UKD and its pulp-UKP, sweetened “kunun-zaki” drink-SKD, and its...
Assessment of genetic diversity in Nigerian local chicken population based on haematological parameters
March 2025
Assessment of genetic diversity in Nigerian local chicken populations based on haematological parameters was conducted using 150 chickens, comprising 50 Normal-feathered (NF), 50 Frizzled-feathered (FF), and 50 Naked-neck (NN) birds. The chickens were reared under a deep litter system and allowed to acclimatize for four weeks. Males and females were kept separately to prevent mating during the research period. Blood...
Phenotypic and genotypic evaluation of common bean lines (Phaseolus vulgaris L.) developed for multiple disease resistance
February 2025
A field experiment was conducted at the Tanzania Agricultural Research Institute (TARI-Selian) to assess the resistance of seventeen improved common bean genotypes against four major foliar diseases: angular leaf spot, bean common mosaic virus, common bacterial blight, and anthracnose. The experiment was laid out in a randomized complete block design (RCBD) with three replications. Disease ratings were scored...
Chemical composition, physicochemical parameters and nutritional value of Lannea velutina A. Rich (Anacardiaceae) seed and seed oil
January 2025
A study on Lannea velutina seeds was carried out to establish their chemical compositions. Proximate composition, physicochemical parameters, fatty acids, tocopherols, minerals, amino acids, simple sugars, phytates, fibers and polyphenols of seed kernel and seed oils were evaluated using the pre-established methods. The oil content was 57.3%. Oleic acid (43.9%), palmitic acid (34.9%) and linoleic acid (12.7%) were the...
Receiver operating characteristic (ROC) and R fitting curve for quantifying rhizobacteria and foliar bio-fertilizer combination performances on maize crop growth process in an arid soil
December 2024
Studies have suggested that microorganisms in the rhizosphere have the potential to improve soil fertility without causing any environmental problems. Previous studies have shown a synergistic interaction between these microorganisms and foliar bio-fertilizers as an acceptable practice for improving maize and soybean growth and yield in the arid agro-climate of Korhogo, Northern Côte d'Ivoire. In this study,...
Induction of flowering in cassava (Manihot esculenta Crantz) using plant growth regulators, pruning and extended photoperiod through night-breaks
December 2024
Cassava is a food security crop in Kenya, whose production is hampered by cassava brown streak disease (CBSD) and cassava mosaic disease (CMD). These challenges require urgent resistance breeding. Crossing of varieties in cassava is disadvantaged by poor and asynchronous flowering. This study aimed to induce and enhance flowering in CBSD immune and resistant clones with variable flowering characteristics. Two...
The determination of chemical composition in some vinegars and compliance with food legislation
December 2024
This paper covers that the six different brands sold (genuine-organic-synthetic apples and grapes) in Turkey and spontaneous fermentation vinegars produced with seven fruits (apple, white and red grapes, mandarin, pear, carrot, melon) in the laboratory had been analyzed according to pH value, acidity, density, the amount of dry matter, color measurement, the ash content and the water activity. The aim of the study was...
In vivo assessment of aqueous extract of African locust beans pulp-mango peel blend as a potential alternative natural sweetener for Alloxan induced diabetic rats
November 2024
The search for safer and more nutritious natural sweeteners as alternatives to commercial sweeteners, which are implicated in obesity and diabetes mellitus, has led to the utilization of African locust bean (Parkia biglobosa) pulp, which is rich in dietary fibers, soluble sugars, and vitamins. Mango peels, containing antioxidants, fiber, and nutraceuticals, were also used. The pulp and mango peels were blended and used...
Molecular detection of Schistosoma haematobium infection among school children in Sabon-Gari Local Government Area, Kaduna State, Nigeria
November 2024
Urogenital schistosomiasis is the most prevalent type of schistosomiasis, leading to terminal haematuria and bladder wall pathology. The disease is often characterized by blood in urine, affecting 110 million individuals globally. This study was conducted to determine the prevalence and associated risk factors of Schistosoma haematobium infection, the major pathogen of the disease, among school-age children in Sabon...
Identification and molecular characterization of Caryedon furcatus (Anton & Delobel), the main pest of Senegalia macrostachya (Reichenb. ex DC. Kyal & Boatwr) seeds in storage in Burkina Faso
October 2024
In Burkina Faso, seeds of Senegalia macrostachya (Rchb. Ex DC.) Kyal. & Boatwr. during storage are attacked by a pest morphologically identified as Caryedon mauritanicus Auct. It was described for the first time in 2004 under a new name 'Caryedon furcatus' by Anton and Delobel. Thus, C. mauritanicus is now considered to be C. furcatus. In the present study, for the first time, genetic characterization of C....
Morphological, biochemical and sensory characterization of some cocoa genotypes (Theobroma cacao L.) grown in Cameroon
October 2024
Cameroon’s cocoa is derived from a wide variety of genetic material. These genotypes are not adequately classified in terms of the quality of cocoa produced. The aim of this study was to help improve cocoa quality by selecting genotypes with organoleptic properties. An assessment was conducted on the agro-morphological, biochemical and sensory characteristics of traditional 'German cacao' genotypes (GR and...
Zootechnical characteristics and ovarian potential of Kouri cows slaughtered in the Lake Chad Region
October 2024
The present study was conducted on the zootechnical characterization and ovarian potential of Kouri cows slaughtered in four slaughterhouses in the Lake Chad region. For this purpose, a total of 100 cycled cows and 20 pregnant cows were used. Before each cow was slaughtered, its age, weight and body condition score were determined. At the slaughterhouse, the ovaries were removed and transported in a portable cooler at...
Estimates of genetic parameters and agronomic performance of spider plant (Cleome gynandra) in three climatic zones of Burkina Faso
October 2024
Cleome gynandra L. is one of the leafy vegetables traditionally appreciated in the human diet, with interesting nutritional and therapeutic values. Its cultivation generates substantial income for market gardeners. To make the most of this important resource in the country's different climatic zones, we need to know more about its agronomorphological performance and estimate its genetic parameters. The aim of the...
Enhanced rooting and regeneration of canola (Brassica napus L.) cultivars after a brief period (shock) on growth regulator-free Murashige and Skoog (MS) medium
September 2024
The comparative organogenesis of Brassica napus L cultivars Cyclone, Star and Westar was studied. The cotyledonary explants gave a higher response to all the combinations of 0.5 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) and benzylamino purine (BAP; 0.5, 1.0,1.5 and 2.0 mg/L) used for optimizing the conditions for callus induction. The best mean weight and mean length of callus was obtained at 0.5 mg/L 2,4-D and 1.5...
Assessing genetic variability induced in chickpeas by gamma rays
August 2024
Mutation breeding is a useful technique for discovering desirable traits in chickpea genomes. Therefore, the overall objective of this study was to bring out genetic variability in the genotype of chickpeas by treating the seeds with six doses (0.0, 25, 50, 100, 200, 400 and 800 rad) of gamma irradiation to generate genetic variations in morphological traits and yield components to bring out information about the...
Molecular characterisation for resistance to leaf spot disease in some local groundnut ecotypes of Burkina-Faso
August 2024
Leaf spot disease is the major constraints causing yield losses in groundnut. Numerous breeding programmes have been set up to select disease-resistant varieties, but the selection pressure exerted by resistant varieties encourages the development of new biotypes. It is therefore necessary to use biotechnology to identify traits of interest. The general objective of this study is to identify SSR markers associated...
Effect of planting dates on the quantitative traits of Solenostemon rotundifolius [(Poir.) J. K. Morton]
July 2024
The present study aimed to evaluate the effect of the planting date on quantitative traits of S. rotundifolius. To this end, twelve accessions of S. rotundifolius, including six (6) from Burkina Faso and six (6) from Ghana, were subjected to agro-morphological evaluations using a Fisher block design with three replications on three dates: April 3rd (D1); June 3rd (D2) and July 8th (D3). Fourteen (14) quantitative traits...
Influence of soil physical properties, chemical contents and rhizobacterial loads on soil quality in maize fields of Southwestern Nigeria
July 2024
In Southwestern Nigeria (SWN), the high demand for maize has resulted in increased levels of maize cultivation. However, continuous maize cultivation is directly or indirectly affecting soil nutrient reserves. This study therefore assessed and compared the soil physical conditions, chemical contents, and rhizobacterial loads of soil samples collected in maize fields across the five (Guinea Savannah-GS, Derived...
Assessment and validation of reference genes for qRT-PCR normalization in local cowpea (Vigna unguiculata L.) varieties in response to sterilized and unsterilized soil conditions
June 2024
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is widely employed as a mandatory analysis in biological work to analyze and validate gene expression; however, its precision is greatly hampered by the robustness of the internal control genes employed for normalization. In this study, we selected suitable housekeeping genes in leaves of cowpea for normalization of expression analysis when...
Modifications of growth, mineral uptake, chlorophyll content, osmolyte contents, antioxidant compounds and yield of three varieties of yam (Dioscorea rotundata L.) in saline conditions
June 2024
A vast area of the world is negatively impacted by salinity which causes loss of crop yields. Modifications in the growth, mineral uptake, chlorophyll content, osmolyte contents, antioxidant compounds and yield of three varieties of yam (Dioscorea rotundata L.) were evaluated in saline conditions, in a greenhouse (0, 50, 100 and 200 mM) and farm (0 and 50 mM). The leaf area, stem height, noose diameter, chlorophyll...
Optimization of the sensorial quality of Saka Saka, a dish made from cassava leaves
May 2024
The goal was to improve Saka Saka's sensory quality. The study analyzed Saka Saka samples by pre-treating cassava leaves, mixing ingredients, and optimizing color, odor, and taste. The data was analyzed using Minitab 17.3.1 software, with replicates varying. The sensory evaluation of Saka Saka samples showed acceptable characteristics, with scores ranging from 5 to 8. The most appreciated characteristic was color,...
Pathogenic and molecular characterization of Pseudocercospora griseola, the causal agent of angular leaf spot of common bean, in Eastern Democratic Republic of Congo (DRC)
May 2024
Angular leaf spot (ALS) caused by Pseudocercospora griseola is the most economically damaging common bean (Phaseolus vulgaris L.) foliar disease in Democratic Republic of Congo (DRC) and elsewhere in Eastern and Central Africa. Information regarding pathotypes distribution and genetic structure that would enable development of appropriate control measures is still lacking in DRC. The study aimed to investigate the...
Effect of incorporating the pulp of the fruit of Detarium microcarpum Guill & Perr. on the quality of the traditional malt-based drinks in West Africa
May 2024
Detarium microcarpum is a forest plant widely used in West Africa. However, it is little known and used for food. This study aims to assess the beneficial effect of incorporating the pulp of the fruit of D. microcarpum on the quality of the traditional malt-based drinks. Thus, the objective was to produce traditional drinks based on composite flour of D. microcarpum pulp and malt, then to characterize these drinks on...
Isolation and binary fusion of taro (Colocasia esculeneta (L) Schott.) protoplast: Towards developing somatic hybridization protocol as an alternative to sexual hybridization
April 2024
Collocasia esculenta (L) Schott. (Taro) genotypes cultivated in Kenya rarely produce flowers, thus improvement via pollination is hindered. Somatic hybridization is an attractive alternative to circumvent flower pollination constrain and hence explored in this study. The aim of this study was to optimize isolation and binary fusion of protoplast obtained from C. esculenta (Dasheen) and C. antiquorum Eddoe) genotypes....
Evaluation of runs of homozygosity and genomic endogamy in the Creole breeds Guaymi and Guabala in Panama
April 2024
The inbreeding coefficient measures the likelihood of identical alleles at a locus in a population due to descent from a common ancestor, highlighting potential negative impacts on health and fitness in both natural and domesticated populations. This study focuses on homozygous segments continuous genomic regions of homozygosity resulting from the inheritance of identical haplotypes from both parents and their role in...
Evaluation of the seroprevalence of okra mosaic virus in Koulikoro, Mali
April 2024
Mosaic virus disease is transmitted by several types of viruses such as OMD, OYVMV, OLCV, CLCuGV, and CYCrV. Among these viruses, some of them are significant threats to okra production worldwide. To develop an integrated pest management strategy against this disease, this seroprevalence study was conducted at the Rural Polytechnic Institute (RPI) of Katibougou, Koulikoro region, Mali. Symptomatic leaves of okra plants...
In vitro antifungal potential of citral and nanoencapsulated citral against Fusarium oxysporum f.sp. lycopersici
April 2024
This study aimed to encapsulate citral in chitosan nanoparticles and test its antifungal activity, along with the nanoparticles, against Fusarium oxysporum f.sp. lycopersici (FOL), a soil-borne pathogen causing worldwide yield losses in tomato production. The nanoparticles were characterized using scanning electron microscopy (SEM), FT-IR, encapsulation efficiency (EE), loading capacity (LC), and in vitro release...
Genetic diversity of Fusarium isolates infecting potato (Solanum tuberosum) and tobacco (Nicotiana tabacum) crops in Zimbabwe
March 2024
Potato (Solanum tuberosum) and tobacco (Nicotiana tabacum) are important crops that contribute significantly to Zimbabwe’s economic development. However, they are attacked by Fusarium species leading to reduced yields, quality and profitability for farmers. A study was conducted to investigate the diversity of Fusarium spp. causing wilt and rots in both crops in Zimbabwe. Plants displaying symptoms of wilting,...
Effects of seed coat pigmentation on germination characteristics and antioxidant properties of Argyrolobium uniflorum ((Decne.) Jaub. & Spach) in southern Tunisia
March 2024
Seeds of Argyrolobium uniflorum (Fabaceae), a spontaneous plant in arid and semi-arid regions of Tunisia, showed a color variability in their seed coats (green, orange, yellow and brown). The influence of the color of seed coat on germination and phytochemical seed composition was evaluated. Germination tests were carried out and the results obtained show that the applied chemical treatment (sulfuric acid) improved...
Detection of virulence genes and antibiogram of Shiga toxin-producing Escherichia coli O157:H7 from slaughtered cattle and abattoir effluent in Zaria, Nigeria
March 2024
Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a formidable human pathogen causing food-borne diseases worldwide. The present study investigates virulence genes and determines the antimicrobial characteristics of STEC O157:H7 isolates from faecal and effluent samples at Zaria abattoir in Zaria. PCR assays were used to detect the virulence genes of STEC O157:H7 isolates. The isolates that produced stx genes...
Screening of tuberculosis in highly exposed children in the population of Cameroon central region
March 2024
Although the treatment for tuberculosis (TB) is available nowadays, it remains a real concern, especially since cases in children are often detected late and underreported. Studies have shown that elevated serum levels of C-reactive protein (CRP) are associated with TB in adults. This study aims to assess the CRP levels among the pediatric cases at Jamot Hospital (Yaoundé) and Meguemessi community in Cameroon....
Systematic studies on tetra SL hybrid hen technology and rearing for chicken welfare
March 2024
In Serbia, there are 4 to 6 genotypes of hens used for producing eggs for consumption, including hybrid Tetra SL. The proper rearing of commercial flocks is critical for timely laying, intensity of laying capacity, number of laid eggs, and period of hen usage. The aim of this paper is to analyze the results of rearing the offspring of hybrid Tetra SL, from one-day-old chickens to eighteen-week-olds, with a review of the...
Multi-resistance to carbapenems by the production of Imipenemase (IMP)-types carbapenemases in Gram-negative bacilli in Burkina Faso
February 2024
The growing prevalence of Imipenemase (IMP) metallo-beta-lactamases (MBL) producing strains of Gram-negative bacilli (GNB) pathogens is a real concern for clinicians in the light of therapeutic impasses driven. However, resistance genes encoding these enzymes are hardly documented in Burkina Faso. This study aims to show carbapenem-resistance mediated by the production of IMP-type carbapenemase in GNB clinical strains...
The effect of clarification methods on quality attributes of sugarcane-watermelon wine fermented by palm wine yeast (Saccharomyces cerevisiae)
February 2024
Clarification of wine is aimed at improving the quality of the product by removing haze. In this study, the effect of two clarification methods namely membrane filtration technique and the use of Keiselguhr diatomaceous earth powder on the physico-chemical and sensory attributes of wine produced using sugarcane (Saccharum officinarum L) and watermelon (Citrullus vulgaris L) juice blended in the ratio 1:1 (v/v)...
Establishment of an efficient regeneration protocol of six different varieties of wheat (Triticum aestivum L.)
February 2024
An efficient callogenesis and regeneration protocol was optimized for six different wheat (Triticum aestivum L.) varieties including Chakwal-50, Galaxy-01, NARC-09, Pakistan-13, Millat-11, and Borlog-14. For callogenesis, mature seeds of each variety were used as explant, surface sterilization, and culturing were carried out on MS medium enhanced with dissimilar levels (0.5, 1.5, 2.5, 3.5, 4.5, and 5.5 mg/L) of...
Distribution of cassava mosaic begomoviruses in the North-Western provinces of Democratic Republic of Congo
February 2024
This study was carried out for the first time in the North-Western Democratic Republic of Congo (DR Congo) as part of the Viral Epidemiology in West and Central Africa (WAVE) program. Its dual aim was to identify and map the viruses responsible for African cassava mosaic in this part of the country. Cassava leaf samples were collected during a geo-referenced survey conducted from 1st February to 31st March, 2022 in...
Analytical performances description of the immunoturbidimetric method for the determination of HbA1c using Selectra Pro M automated system at the Institut National d'Hygiène (INH) of Lomé
January 2024
HbA1c is the best indicator for monitoring glycemic control in both type 1 and type 2 diabetic patients. Its value allows us to predict the development of long-term degenerative complications of diabetes. Several techniques offered by different laboratories allow the determination of HbA1c based on different principles. The purpose of the present study was to perform the verification of the analytical performances of...
Purification of stilbene synthase from Phaseolus vulgaris and determination of its catalytic activity
January 2024
Stilbene compounds, secondary metabolites produced by plants in response to external environmental stimuli, are garnering increasing attention. Stilbene synthase (STS) plays a crucial role in their synthesis. This study focuses on the purification of stilbene synthase from Phaseolus vulgaris and the determination of its catalytic activity. The stilbene synthase gene obtained from the Phaseolus genome was expressed in...
Typing of enterovirus identified from Moroccan mussels (Mytilus galloprovincialis Lamarck, 1819) by seroneutralization
January 2024
Non-poliovirus enteroviruses (NPEV) are infectious agents which can determine various illness in human such as hand-foot-mouth syndrome, angina, respiratory diseases, acute or chronic heart disease, diarrhea, pancreatitis, acute hemorrhagic and conjunctivitis. These viruses are eliminated in the stool and thus contaminate the marine environment and shellfish. In Morocco, shellfish sanitary quality analysis does not...
Estimates of genetic variability in ultraviolet irradiated populations of summer squash
January 2024
Summer squash is an important member of the family Cucurbitaceae. It is one of the most popular vegetable crops cultivated in Egypt. Increasing yield and improving fruits quality are the targets for growers. Therefore, this study aimed to evaluate the extent of genetic variations, heritability, as well as expected genetic advance for growth and yield related traits in four genotypes of summer squash subjected to three...
Morphological traits variation of cowpea (Vigna unguiculata L. Walp) grown in Côte d’Ivoire
January 2024
The genetic diversity of a genetic resource is an indicator of its richness and offers many possibilities for improvement. Cowpea is an important source of nutrients for humans and animals. This study aimed at assessing genetic diversity and relationships present among 135 accessions in the Centre National de Recherches Agronomiques (CNRA) collection, using qualitative traits. Traits analysis revealed significant...
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