African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12370

AJB Articles

Rapid single-tube splice variants typing of the BF gene based on dual-primer RT-PCR amplification that influence resistance/susceptibility to Marek’s disease in chicken

January 2022

A new effective splice variants typing based on multiplex allele-specific dual-primer RT-PCR assay was developed in a single tube for the rapid detection of the exon 7 splice variant of the BF gene. With 2 pairs of primers, one pair was used for amplifying cDNA fragments containing exon 7 of the BF gene, the other does not contain exon 7 of the BF gene. The templates were amplified in one tube and the type of splice...

Author(s): Yuan-chang Jin, Yu-feng Li, Liang Huang, Jia-jun Zhou, Xue-fang Zhang,  Ran Ma, Meng-lin Lu, Mei-lin Hao, Gang Zeng  and Bo-ping Zeng

Effect of foliar application of nitrogen-phosphorus-potassium fertilizers on nutrient uptake and protein content of maize

December 2021

The effect of foliar application of nitrogen-phosphorus-potassium (NPK) fertilizers on nutritional status and yield of maize (Zea mays L.) were investigated through a study conducted in a zonal agricultural research station of University of Agricultural Sciences, GKVK Bengaluru (India). The experiment was laid out in randomized complete block design. The treatments consisted of two levels of soil application (75 and...

Author(s): D. Nirere, S. R. Mbaraka, F. X. Rucamumihigo, F. Murorunkwere, F. R. Musana and H. Rukangantambara

Statistical modeling of effective doses in hormetic dose-response relationships by reparameterization of a bilogistic model for inverted U-shaped curves

November 2021

Hormesis is a biphasic dose (concentration)-response phenomenon characterized by low dose stimulatory and high dose inhibitory effects exerted by stressors on living organisms. Recently, there has been increased interest in the phenomenon and statistical models for its exploration in toxicological studies. The bilogistic model of Beckon et al. is one of such models for modeling biphasic dose-response relationships in...

Author(s): Christian O. Nweke, Oluchukwu R. Nwangwu, Chukwudi J. Ogbonna and Reuben N. Okechi

Advances on the application of non-coding RNA in crop improvement

November 2021

Thanks to committed plant breeding researchers over the few decades, many problems associated with food supply and qualities have been improved. Food security is an exceptionally serious worldwide issue via world climate change, the increase in human population, and the use of plants for bioethanol production in current years. Improved tolerance to abiotic and biotic stress, resistance to herbicides, improved yield, and...

Author(s): Diriba Guta Dekeba

Genetic diversity analysis of sorghum [Sorghum bicolor (L.) Moench] races in Ethiopia using simple sequence repeats (SSR) markers

November 2021

Sorghum [Sorghum bicolor (L.) Moench], is believed to be originated in Ethiopia and Sudan. Although, many morphological and molecular diversity studies reveal the existence of genetic variations with sorghum populations, their distribution within basic races were not considered. Hence, the present study aimed to analyze the extent and distribution of genetic variation within basic Ethiopian sorghum landraces using SSR...

Author(s): Gamachu Olani, Tileye Feyissa and Tesfaye Disasa

Antioxidative and anti-aging activities of Abeliophyllum distichum Nakai extracts fermented with lactobacillus plantarum and lactobacillus brevis

October 2021

Abeliophyllum distichum Nakai is a deciduous shrub that belongs to Oleaceae Abeliophyllum and grows only in Korea. In this study, the antioxidative activity, cytotoxicity, anti-aging activity, and levels of acteoside and isoacteoside were compared between non-fermented and fermented (using Lactobacillus plantarum and Lactobacillus brevis) extracts of A. distichum Nakai. The cytotoxicity of these extracts, along with...

Author(s): KiBeom Lee, Ju Hyun Park and Yun Sung Kim  

Spatial and regional directory of wild Auricularia species found in the forest of Southwest Nigeria

October 2021

The African forest, which was and still is the heart of biodiversity, is fast losing its bio-components due to natural disasters and human encroachment. A catalogue of the bio-components of the African forest is imminent so that conservation programs can be established to safeguard rare germplasm(s) from extinction. The population density of wild Auricularia (Mushroom) of Southwestern Nigeria was the focus of this...

Author(s): Victor S. Ekun, Clementina O. Adenipekun, Omena B. Ojuederie and Peter M. Etaware

Assessment of water requirement for rice crop at Cyabayaga wetland in Nyagatare District, Eastern Province, Rwanda

October 2021

The overall objective of this present research is assessment of rice-water requirement in Cyabayaga wetland of Nyagatare District in Rwanda. Specific objectives of this research project were to collect and analyze weather parameters, of soil physical properties, crop-water requirements and to recommend efficient water management of the study area. Research design, data collection and field observations were made during...

Author(s): Valens Nkundabashaka, Sylvestre Habimana, Rose Niyonkuru, Claude Kayijuka, Antoine Karangwa, Saidi  Rumanzi Mbaraka, Fabrice Rwalinda Musana, Eularie Mutamuliza and Marie Chantal Mutimawurugo

Antioxidant effects of ginger, garlic and onion aqueous extracts on 2-thiobarbituric acid reactive substances (2-TBARS) and total volatile basic nitrogen (TVB-N) content in chevon and pork during frozen storage

October 2021

The study investigated the effect of 10% aqueous extracts of ginger, garlic and onion on the quality and shelf life of frozen chevon and pork as reflected by changes in values of 2-thiobartituric acid reactive substances (2-TBARS) and total volatile basic nitrogen (TVB-N) over a 14 day storage under frozen conditions.  The pH, 2-TBARS and TVB-N of chevon and pork were measured from frozen storage at -20°C after...

Author(s): Lesten Eliez Chisomo Chatepa, Kingsley George Masamba and Jonathan Tanganyika

Efficient conditions for in vitro establishment and regeneration of disease-free Ugandan farmer-preferred cassava genotypes

September 2021

Cassava (Manihot esculenta Crantz) is majorly devastated by two viral diseases, cassava brown streak disease (CBSD) and cassava mosaic disease (CMD), resulting in 100% yield loss. Being a clonal plant, nodal cuttings (NC) and shoot apical meristems (SAMs) are the best explants for production of disease free planting materials. In this study, NCs and SAMs were used to determine reliable indicators for successful in vitro...

Author(s): Hellen B. Apio, Titus Alicai and Emmanuel Ogwok,  

Vertical-horizontal subsurface flow hybrid constructed wetlands for municipal wastewater treatment in developing countries: A review

September 2021

The use of low-cost on-site wastewater treatment technologies, including constructed wetlands (CWs), is wide spread. Despite the purported high performance of vertical-horizontal subsurface flow (V-H SSF) hybrid CWs, data on implementation and performance in developing countries is scarce. Here, the design, operation and performance of V-H SSF hybrid CWs for treatment of municipal sewage in an effort to encourage and...

Author(s): Sylvie Muwanga Tebitendwa and Ashton Keith Cowan

Sales environment, microbiological and biochemical quality of beef skins intended for human consumption

September 2021

The quality and composition of meat and its derivatives are influenced by many factors namely physicochemical, organoleptic factors and microbial contamination. The aim of this study was to evaluate kpakouma consumption risk through its chemical and microbiological contaminants. The methodological approach was composed of (i) observation of selling environment, (ii) pesticides and antibiotics residues quantification by...

Author(s): Haziz Sina, Kamirou Chabi-Sika, Abdou Hamidou Soule, Durand Dah-Nouvlessounon, Bawa Boya, Chantal M. Y. Viwakinnou, Virgile Ahyi, Farid Baba-Moussa, Adolphe Adjanohoun and Lamine Baba-Moussa

Adenine sulfate and glutamine enhanced shoots multiplication in Jatropha curcas L. a potent biofuel plant

September 2021

Jatropha is a drought-tolerant plant producing seed oil for the biodiesel. Limitation to the development of jatropha is unavailability of high-yielding varieties and efficient in vitro regeneration system which is required for micropropagation. In this study, in vitro regeneration system from jatropha juvenile cotyledon was established. Firstly, concentrations of hormones in the MS medium were optimized and it was found...

Author(s): Ismail A. Mohammed, Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukui

Reconstruction of metabolic pathways in selected bacterial and yeast strains for the production of bio-ethylene from crude glycerol: A mini review

August 2021

The role of bioengineering in industrial processes cannot be ignored, especially in circumstances where the processes are uneconomical, demand high energy, use finite resources and emit huge amounts of carbon dioxide. Ethylene is a two-carbon unsaturated hydrocarbon that is industry's most important building block for polyester fibres, plastics, and ethylene glycol. For decades, ethylene production has relied on...

Author(s): Idan Chiyanzu

Phytochemical and in-vitro antioxidant properties of ethyl acetate leave extract of Dryopteris dilatata on Wistar rats

August 2021

Medicinal plants contain natural bio-active constituents in various parts called photochemical that are of therapeutic importance. This research was undertaken to determine the phytochemical constituents and in-vitro antioxidant activities of ethyl acetate extract of Dryopteris dilatata leaves (DDL). Phytochemical analysis was determined on the plant samples following established protocols. The antioxidant property of...

Author(s): Akpotu Ajirioghene, Ani Celestine, Agiopu Toby, Okeke Adaobi, Agu Francis, Ugwuchukwu Nnaemeka, Ugwudike Patrick, Eze Chukwuka, Uzoigwe Jide, Anyaeji Pamela and Ghasi Samuel

Survival rate of donkey foals: Status quo and improvement methods

August 2021

The industrial improvement of donkey-hide gelatin and the development of related products, such as donkey meat, donkey milk, and donkey-hide gelatin cake, drive the upstream donkey breeding industry. However, the shortage of donkey germplasm resources seriously restricts the further development of the donkey industry. Under the biological limitations of long breeding cycle, slow breeding, low conception rate, high...

Author(s): Yonghui Wang, Tianqi Wang, Zhengwei Zhang and Changfa Wang

Physiological and biochemical traits, antioxidant compounds and some physico-chemical factors of Spirulina platensis cultivation as influenced by Moringa oleifera leaves extract culture medium enriched with sodium bicarbonate and kanwa

August 2021

The prospects for using Moringa oleifera leaves extract (MLE) supplemented with different concentrations of kanwa or sodium bicarbonate (NaHCO3) as a low-cost alternative growth medium of Spirulina platensis were evaluated in a small-scale outdoor cultivation system. The present study was aimed to evaluate the potential of MLE growth medium enriched with different concentrations (4 or 8 g L-1)...

Author(s): Wamba Fotsop Oscar, Magwell Rodrigue Pierre Fils, Tchoffo Djoudjeu Kennedy, Bahoya Joseph Aime, Minyaka Emile, Tavea Marie-Frédéric, Lehman Léopold Gustave and Taffouo Victor Désiré

Effect of light quality on in vitro germination, seedling growth and photosynthetic pigments production in wheat (Triticum aestivum L.)

July 2021

Due to its economic importance, wheat (Triticum aestivum L.) has been the subject of most breeding studies, most of which having as a starting point the in vitro cultures required for initiating different cell and tissues cultures such as protoplasts, ovules and pollen cells. All these procedures are used to obtain new varieties or hybrids regarding the improvement of different qualities such as higher productivity,...

Author(s): Cristian Felix Blidar, Remalia Semiredana Chi?, Sanda Rodica Bota, Georgeta ?erban and Oana Delia St?n??el

An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems

July 2021

Taro (Colocasia esculenta L. Schott) is an important crop for food and nutrition security, incomes, and livelihood of people in developing countries. Its cultivation and productivity have been limited mainly due to a lack of quality planting materials. Therefore, this study developed an effective micropropagation method for two common taro varieties in Kenya (Purple Wild and Dasheen) using apical meristem derived from...

Author(s): Mercy Sinkiyian Kepue, Cecilia M. Mweu, David Muruu Kariuki, Linnet Kerubo, Isaac Njaci, Anne Njoroge, Leah Kago, Benard O. Mware and Sita R. Ghimire

Control of ginseng leaf black spot disease by endophytic fungi

July 2021

Ginseng plants associated with a number of fungi, several of which are reported to protect it from pathogens, thus improving plant growth. This study aimed to screen Panax ginseng leaves for endophytic fungi and to assess these fungi for their efficacy to inhibit ginseng black spot disease caused by Alternaria panax. A total of 256 endophytic fungal isolates were obtained from P. ginseng leaves. Most of the fungal...

Author(s): Chunyuan Zhou, Shiquan Xu, Xiangmin Piao, Meixia Yan, Lili Cui and Yingping Wang

Effect of intercropping aerobic rice with leafy vegetables on crop growth, yield and its economic efficiency

July 2021

Aerobic rice production system provides a sustainable alternative to the traditional rice cultivation. To evaluate the agronomic and economic effect of intercropping aerobic rice with four leafy vegetables, field experiments were conducted at University of Agriculture Science Bangalore research station, India during 2017 and 2018. The experiments consisted of 9 treatments with 4 replications and, a Randomized Completely...

Author(s): Sylvestre Habimana, Saidi Rumanzi Mbaraka, Antoine Karangwa, Francois Xavier Rucamumihigo, Fabrice Rwalinda Musana, Eularie Mutamuliza, Ivan Gasangwa, Christian Nkurunziza, Jean Claude Abayisenga and Athanase Rusanganwa Cyamweshi

Changes in some behavioral, hematological and biochemical indices of air-breathing Clarias gariepinus [BUCHELL, 1822] exposed to pharmaceutical effluent

July 2021

This work aimed at evaluating the behavioral, biochemical and hematological effects of pharmaceutical effluent in laboratory population of Clarias gariepinus using a static renewal bioassay system. Fish specimens were collected and exposed to five (0.04, 0.06, 0.08, 0.10 and 0.12 mgL-1) sublethal concentrations of the effluent including a control experiment. Different dose dependent behavioral responses such as erratic...

Author(s): Ogundiran M. A., Ayandiran T. A., Olasunmibo A.O., Olanipeku A. S. and Okaseun O. T.

CRISPR/Cas genome editing: A frontier for transforming precision cassava breeding

June 2021

Improved agricultural production of essential crops through advanced breeding is important for increasing access to nutritious food for the world's rapidly growing population, which is expected to reach 9.8 billion by 2050. Recent advancements in the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein9 (CRISPR/Cas9) genome editing process, which uses single-guide RNA for genome...

Author(s): Bicko Steve Juma, Cecilia Mweu, Mathew Piero and Wilton Mbinda,

Vigna radiata (mung bean) acid phosphatase is difficult to purify and may have a role as a vegetative Storage protein

June 2021

Acid phosphatase from mung bean (Vigna radiata) germinated seeds was partially purified via ammonium sulfate precipitation and fully purified via gel excision and heat denaturation, yielding a 29 kDa protein. Using 4-nitrophenylphosphate as a substrate, Vmax and Km values of 0.10 µmol/min and 0.27 mM, respectively, were obtained. The acid phosphatase was heat resistant, enhanced by the presence of Fe2+ and Mn2+...

Author(s): Hailey Elizabeth Lightle, Sarah Anne Fosco, Rachel Nicole Giles, Jordyn Marie Meekma, Nicole Marie Moore, Megan Vawn Palacio and Suzanne Elisabet Williams

Imaging from anatomic to molecular and atomic resolution scales: A review

June 2021

Imaging may be referred to as the ‘eyes of science’ as it provides scientists with highly informative multi-dimensional and multi-parameter data usually invisible to the naked eye. As instrumentation technologies and genetic engineering advances, it’s possible in modern times to observe and image highly dynamic biochemistry processes. This paper reviews positron emission tomography (PET), magnetic...

Author(s): Kamweru, Paul Kuria

Antimicrobial potentials of idiolites of endophytic fungi isolated from the leaves of Dacryodes edulis

June 2021

The growing levels of antimicrobial resistance have triggered interest in the search for alternative compounds to wage the war against it. Plants have been shown to harbor endophytic fungi that produce bioactive idiolites, which have potential lead compounds in the development of new drugs. This study evaluated the antimicrobial activity of endophytic fungi isolated from leaves of Dacryodes edulis. Endophytic fungi...

Author(s): Malachy C. Ugwu, Dave E. Ochiaja, David C. Nwobodo, Collins Chimezie and Chiamaka B. Ugwu

Effect of VIUSID-Agro™ on the conversion of somatic embryos of coffee (Coffea arabica L.) cv. Red Caturra rojo-884

June 2021

A critical factor in the in vitro propagation protocol via somatic embryogenesis of coffee is the survival and growth of plants under ex vitro conditions. The objective of this work was to determine the effect of VIUSID Agro™ during the conversion of somatic embryos of coffee cv. Red Caturra rojo-884 in semi-controlled culture conditions. Two solutions (0.5 and 0.8 mL L-1) of the biostimulant were evaluated on in...

Author(s): Laisyn Posada-Pérez, Raúl Barbón Rodríguez, Alina Capote Pérez, Anabel Pérez Pérez, Yenny Padrón Montesino, Bulent Kukurtcu, Dion D. Daniels, Guillermo Reyes and Rafael Gómez-Kosky

Efficacy of thymol and eugenol against bacterial wilt bacterium Ralstonia solanacearum

June 2021

Bacterial wilt caused by Ralstonia solanacearum is a major constraint to production of potatoes (Solanum tuberosum L). To date, there are no known conventional bactericides which provide effective control of this soil borne pathogen. This study aimed at investigating the antibacterial activity of eugenol and thymol which are the major components of thyme and clove essential oils, respectively against R. solanacearum....

Author(s): George Oluoch, Edward George Mamati, Viviene Matiru and Moses Nyongesa

Quantification and characterization of faecal sludge produced in Kara

May 2021

In Kara (Togo), as in most major sub-Saharan African cities, there is very little sewerage and autonomous systems are the most commonly used. These autonomous systems produce faecal sludge that must be drained and treated adequately in order to preserve the health of the population and protect the environment. Indeed, the risks are real, given the use of fresh faecal sludge in agriculture and the potential dangers of...

Author(s): Piyabalo KODOM, Edem Komi KOLEDZI, Nitale M’Balikine KROU, Kwamivi N. SEGBEAYA and Bouwèdèo TOI BISSANG

Synthesis, physicochemical and antimicrobial properties of rhenium (I) tricarbonyl complexes of isatin Schiff bases

May 2021

The Schiff base of isatin and sulphanilamide (L1; C14H11N3O3S) was prepared and reacted with Re(CO)5X (X = Cl and Br) in toluene to give [Re(C14H10N2O)(CO)3X] (X = Cl, 1 and X = Br, 2). The prepared Schiff base of isatin and 4-methoxyaniline (L2; C15H12N2O2) was reacted with Re(CO)5X in toluene to give [Re(C14H10N2O)(CO)3X], where X = Cl, 3 and X = Br, 4. L2 and Re(CO)5Br were refluxed in dry toluene under nitrogen and...

Author(s): Adebomi A. Ikotun, Abimbola A. Owoseni and Gabriel O. Egharevba  

Precise assessment of angular leaf spot severity using the Leaf Doctor app for common beans

May 2021

Angular leaf spot (ALS) is one of the most severe diseases of common bean. However, there is as yet no standardization of the phenological stage, inoculum concentration, diagrammatic scale (DS), and day of assessment for screening ALS resistance in bean lines. The present study aimed to evaluate the potential for assessment of ALS severity in common beans using the Leaf Doctor app and inoculation at the V2 phenological...

Author(s): Caléo Panhoca de Almeida, Gabriel Francesco Janini Bonfante, Jean Fausto de Carvalho Paulino, Bianca Cristina de Deus, Flávia Rodrigues Alves Patrício, Sérgio Augusto Morais Carbonell, Alisson Fernando Chiorato and Luciana Lasry Benchimol-Reis

Synthesis, characterization and antibacterial activity of AgNPS mediated by Ocimum sanctum

May 2021

This research focused on isolation of Silver Nano Particles (AgNPs) using leaf filtrate of Ocimum sanctum and evaluation of their antibacterial action against Bacillus aureus, Staphylococcus aureus and Escherichia coli. It is known that plant extracts act as reducing agents, stabilizers and as capping agents. The O. sanctum plant was specifically selected due to its medicinal properties and abundant availability. The...

Author(s): A. V. Narasimha Swamy and R. Srinu Venkat Rao

Production of truncated peptide (cellobiohydrolase Cel6A) by Trichoderma reesei expressed in Escherichia coli

May 2021

Enzymatic cellulose hydrolysis is an important step for the production of second-generation biofuels. The filamentous fungus Trichoderma reesei is among the most important organisms for obtaining cellulolytic enzymes. The Cel6A (CBH II) cellulase from T. ressei plays an important role in cellulose hydrolysis and acts on the non-reducing end of cellulose, in contrast to Cel7B (CBH I), which acts on the reducing end of...

Author(s): Miriam Shirley Tellez Calzada, Juan Antonio Rojas Contreras, Jesus Bernardo Paez Lerma, Nicolas Oscar Soto Cruz and Javier López Miranda

A rapid detection method on-site for Zygosaccharomyces based on loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick (LFD)

May 2021

The Zygosaccharomyces is notorious for its remarkable spoilage characteristics. In the present study, the visual and rapid identification of the genus Zygosaccharomyces was performed by a loop-mediated isothermal amplification (LAMP) assay using specific primers in Mini Dry Bath within 30 min at 65°C followed by a lateral flow dipstick (LFD) detection. The sensitivity evaluation revealed LAMP-LFD assay with...

Author(s): Yang Liu≠, Jun Wu≠, Li-Qing Chen, Ya Shi, Hong-Xia Zhao, Wei Chen, Hong Li and Lu-Ping He,

Optimization of growth media for maximal production of insecticidal vegetative protein (vip3A) from Bacillus thuringiensis and its activity against black cutworm (Agrotis ipsilon)

May 2021

Bacillus thuringiensis is a Gram-positive bacterium naturally found in soil, water and grain dust. B. thuringiensis has been used for decades as biological control for pests of economical and medical importance. The aim of this study was to test different growth media for the optimal production of vegetative insecticidal protein (Vip3) by B. thuringiensis recovered from the local environment. The local B. thuringiensis...

Author(s): Fatimah Alshehrei, Doaa K. El-Ghareeb, Ashraf El Baz, Sameer Qari, Hanafy Hamza, Hussein H. Abulreesh, Hesham A. Malak, Rehab Elmougy and Gamal E. H. Osman,

Composition and balance of the analytical fractionation of desert date (Balanites aegyptiaca L.) seeds harvested in Senegal

April 2021

The desert date (Balanites aegyptiaca L.) can be one of the most common trees in Senegal. Thus, the objective is to carry out an analytical fractionation of these seeds to consider prospects for valuing the different constituents and a diagram of fractionation processes. The physical characterization of the seeds made it possible to know the fruit-seed and kernel-hull proportions. The chemical composition of the seeds...

Author(s): Djibril Diedhiou,, Mamadou Faye, Laure Candy, Virginie Vandenbossche, Gérard Vilarem, Oumar Sock and Luc Rigal

Molecular characterization of bacteria strians isolated from “JIKO”: A herbal preparation consumed in some parts of Kaduna metropolis, Nigeria

April 2021

Herbal products are considered as phytomedicines which are used as complementary medicine and without adequate monitoring they are leased straightaway into the market. Preparation, transportation and handling of these products can lead to high microbial contamination which can be harmful to human health. The aim of this research was to molecularly characterize some multiple resistance bacteria obtained from JIKO which...

Author(s): Ebipade Kereakede, Mohammed Sani Abdulsalami, Nkechi Eucharia Egbe, Victoria Moltong Yilwa, Enimie Endurance Oaikhena, Elizabeth Omolola Oladapo, Asabe Danjuma and Kingsley Onuh

Investigation of the anti-bacterial properties of mangrove fern, Acrostichum aureum in the Niger Delta, Nigeria

April 2021

Mangroves render several ecosystem services globally, one of which is pharmacological products. This study was done to verify whether Acrostichum aureum, a species of mangrove, common in the Niger Delta has anti-bacterial property. Several leaf samples were plucked from the trees at Eagle Island and placed in a cooler and sent to the lab for further analysis. The leaves were dried and ground into fine powder with manual...

Author(s): Aroloye O. Numbere and Eberechukwu M. Maduike

Serum total protein concentration and liver enzymes activities in albino rats model administered with ethanolic leaf extract of Ficus capensis

April 2021

There is an increased use of the Ficus capensis plant and concerns about safety alongside efficacy have been raised. The aim of this work is to study the total protein, bilirubin and serum liver enzyme activities in albino rats administered graded concentrations of ethanol extract of F. capensis leaf. Twenty-four albino rats were shared into four groups and different concentrations of the leaf extract were administered...

Author(s): Chukwudoruo, Chieme Sunday, Osuji-Kalu-Ibe, Nnenna Chinenyenwa, Igwe, Kalu Okereke, Iheme, Callistus Izunna, Favour Ntite Ujowundu and Mba, Blessing Amarachi

Isolation and characterization of a novel keratinolytic bacterial strain from soil samples of chicken feather keratinaceous-waste dumping sites of Ngaoundere (Cameroon)

April 2021

This study aims at characterizing new chicken feather-degrading bacteria isolated from soils of keratinic waste collected from four dumping sites of Ngaoundere. Fifteen proteolytic bacteria were isolated with three (D1, D2 and F1) showing the capacity to degrade feather in Basal Salt Media. Highest degradation rate (71.11 ± 3.05%) of feather was obtained from isolate D2 from Municipal slaughterhouse. The partial...

Author(s): Sileu Dombou Armelle Leslie, Adjia Zangue Henriette, Nso Emmanuel Jong, Njintang Yanou Nicolas and Tatsadjieu Ngoune Leopold

Brewer’s residues and cocoa pod shells as a substrate for cultivation of Pleurotus ostreatus CCIBt 2339 and enzymes production

March 2021

This work investigated the best composition of a substrate for cultivation of Pleurotus ostreatus CCIBt 2339 using brewer's spent grain (SG) and cocoa pod shells (CP) complemented with hot trub (HT) and/or residual brewer’s yeast (RY). The residue HT was detrimental for cultivation and a substrate composition (%, w/w) of 58% SG + 40% CP + 2% RY – with a C/N ratio of 25.77 g/g – resulted in the best...

Author(s): Carolina Fernandes Pereira, Antônio Zózimo de Matos Costa, Givaldo Rocha Niella, José Luiz Bezerra, Ana Paula Trovatti Uetanabaro and Elizama Aguiar-Oliveira

Adsorption of copper (II) ions onto raw Globimetula oreophila (Afomo ori koko) leaves

March 2021

This study examined the adsorption of copper onto raw Globimetula oreophila leaves. The adsorbent surface nature was examined using scanning electron microscopy and Fourier transform infrared spectroscopy. Adsorption parameters such as the –log10[H+], point of zero charge, initial metal concentration, mass of biomass and contact time were determined. Copper adsorption decreased gradually with pH. The point of zero...

Author(s): Chijioke John Ajaelu and Esther Oremeiyi Faboro

Comparison of homogenization methods for extraction of maize cob metabolites

March 2021

Previous proteomic studies have shown maize cob tissue to have an essential role in pathogen defense. Currently, there are no studies published regarding neither maize cob metabolomic profiles nor an accepted method of metabolite extraction from cob. This study assesses the reproducibility of metabolite extraction from the cobs of fungal pathogen -resistant and -susceptible cultivars. Hand grinding, mechanical...

Author(s): Jeremy Winders and Tibor Pechan

Analysis of the degradation of polyethylene, polystyrene and polyurethane mediated by three filamentous fungi isolated from the Antarctica

February 2021

Plastic polymers are petroleum-derived synthetic materials that have multiple uses in everyday life, but their excessive production has led to the accumulation of approximately 1,000 million tons of residues, causing negative ecological impacts. This study analyzed the biological degradation in liquid medium of polyurethane, polystyrene, and polyethylene samples by filamentous fungi isolated from Antarctica. The plastic...

Author(s): Rodrigo Oviedo-Anchundia, Daynet Sosa del Castillo, Jaime Naranjo-Morán, Nora Francois, José Álvarez-Barreto, Alejandro Alarcón, Jhonny Saulo Villafuerte and Milton Barcos-Arias

Response of French bean genotypes to Colletotrichum lindemuthianum and evaluation of their resistance using SCAR markers

February 2021

Common bean anthracnose caused by Colletotrichum lindemuthianum is one of the major biotic constraints to production of French beans (Phaseolus vulgaris L.) in Kenya. This study aimed at screening French bean genotypes in relation to their response to common bean anthracnose in order to identify potential sources of germplasm for breeding. The genotypes were tested in three sites (Kakamega, Mwea and Kutus) where results...

Author(s): Antony Kimani Kamiri, Edith Esther Arunga, Felix Rotich and Reuben Otsyula

Identification of zygotic and nucellar seedling of Harumanis mango through molecular markers and morphological approach

February 2021

Identification of zygotic and nucellar seedlings of Harumanis mango by morphological approach is impossible or hard to be performed. Therefore, this research were to identify zygotic or nucellar seedlings from Harumanis mango polyembryonic seeds through simple sequence repeat (SSR) molecular markers in relation with germination sequence and vigour of seedlings for source of true-to-type cutting material. The...

Author(s): Zul Helmey Mohamad Sabdin, Muhammad Najib Othman Ghani, Shahril Ab Razak, Mashitah Jusoh, Muhamad Hafiz Muhamad Hassan and Ahmad Hafiz Buniamin

A novel genotype-independent technique for successful induction of somatic embryogenesis of adult plants of Jatropha curcas L. using petiole transverse Thin Cell Layer (TCL)

February 2021

Developing a protocol for somatic embryogenesis (SE) for adult mother plants of Jatropha curcas, aids to overcome problems such as harvesting time and an uneven yield, providing the opportunity to propagate proven elite genotypes. Until now, several authors have achieved SE in J. curcas using different explants, however, in none of these protocols adult plants have been used as mother plant material. Furthermore, the...

Author(s): Cristian Nicolás Mendoza-Peña and Anne Kathrine (Trine) Hvoslef-Eide

Expression, purification and testing of zinc metalloproteinase aureolysin as potential vaccine candidate against Staphylococcus aureus

February 2021

Staphylococcus aureus (S. aureus) is a versatile bacterium which exhibits multiple antibiotic resistances. To ameliorate the undesirable diseases causing potential, there is a need to design a protective vaccine capable of stimulating immune response against this pathogen. In a similar study in our laboratory, reverse vaccinology approach was used to nominate potential vaccine candidate genes against S. aureus. Zinc...

Author(s): Samar Solyman, Mohamed Reda, and Amro Hanora

Aspects of mushroom cultivation to obtain polysaccharides in submerged cultivation

February 2021

Medicinal and edible mushrooms have biological properties that are important in promoting health. In the last decades, an increasing number of studies have explored various aspects of the cultivation and metabolism of various species. Many isolated substances have potential for use in medicine and among them are polysaccharides, which have been shown to have physiological properties such as anti-tumor, immunomodulatory,...

Author(s): L. H. Campestrini and Salles-Campos C.  

In vitro daylily (Hemerocallis species) bract multiple shoot induction

February 2021

A bract is a non-inflorescence structure that exists in many plant species. In daylilies, the bract is biologically functional and is the object of the present tissue culture study. After sterilization, bract explants were treated with 6-benzylaminopurine (BA) and thidiazuron (TDZ) that were used individually or in combination in Murashige and Skoog (MS) nutrients medium, under room environmental conditions, to study...

Author(s): Kanyand Matand, Meordrick Shoemake and Chenxin Li

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