African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

AJB Articles

November 2016

Investigating the potential of Aucoumea klaineana Pierre sapwood and heartwood wastes to produce cellulosic ethanol

This work investigates for the first time the different reactivity exhibited by sapwood and heartwood wastes under two conditions: They were pretreated with catalyzed ethanol organosolv in the presence of sulfuric acid and their pulps underwent enzymatic hydrolysis by Trichoderma reesei which causes them to release neutral sugars for the production of cellulosic ethanol.  Aucoumea klaineana Pierre (Okoumé)...

Author(s): Rodrigue Safou-Tchiama, Sébastien Ngwa Obame, Nicolas Brosse, Patrice Soulounganga and Timoléon Andzi Barhé

November 2016

Comparison of seed priming techniques with regards to germination and growth of watermelon seedlings in laboratory condition

Seeds of watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai, cv. Crimson Sweet] were used to investigate the effects of different priming techniques on seed germination and early seedling growth. The seeds were soaked in solutions of 0.2% gibberellin (50 mg L–1 gibberellic acid), 0.2% cytokinin (90 mg L–1 kinetin), 0.2% potassium nitrate (2 g L–1 KNO3), 0.2% calcium nitrate [2 g L–1...

Author(s): Willams Ferreira Souza Barbosa, Fábio Steiner, Leandro Castro Matias de Oliveira and Paulo Henrique and Menezes das Chagas

November 2016

Linkage disequilibrium and association mapping of drought tolerance in cotton (Gossypium hirsutum L.) germplasm population from diverse regions of Pakistan

Drought stress is a major abiotic stress that limits crop production. Molecular association mapping techniques through linkage disequilibrium (LD) can be effectively used to tag genomic regions involved in drought stress tolerance. With the association mapping approach, 90 genotypes of cotton Gossypium hirsutum, from diverse regions of Pakistan were used. The morpho-physiological traits of all genotypes were evaluated...

Author(s): Abdelhafiz Adam Dahab, Muhammad Saeed, Nada Babiker Hamza, Bahaeldeen Babiker Mohamed and Tayyab Husnain

November 2016

Inbreeding depression in crosses of coerulea clones of Walker’s Cattleya (Cattleya walkeriana Gardner)

Orchids are among the most beautiful flowers and endangered due to habitat destruction and over-collection. Cattleya walkeriana is one of the most beautiful flowers joining the small sized plant with medium large and heavily scented flowers. It is widely known and appreciated by its beautiful clones and it has much to offer to breeders because their plants have besides other attributes as small habit and big flowers,...

Author(s): Miléia Ricci Picolo, Ceci Castilho Custódio, Nelson Barbosa Machado-Neto

November 2016

The protective role of honey against cytotoxicity of cadmium chloride in mice

The present study aimed to investigate the honey (HY) protective role in opposition to cadmium (Cd) induced chromosomal aberrations of bone marrow and sperm abnormalities. Forty five (45) adult male albino mice were caged into six groups. Mice were injected, i.p, 300 mg HY/kg and/or 0.67 mg CdCl2/kg b.w for 96 h, separately and alternated. The alternated trials were continued for consecutive eight days. Results show...

Author(s): Asmaa Shahrour, Mohamed Zowail and Khaled Sharafeldin

November 2016

Shelf life improvement of sorghum beer (pito) through the addition of Moringa oleifera and pasteurization

Pito is a traditional alcoholic beverage that is mostly brewed in the three northern regions of Ghana. Although widely consumed and used in many festivities, poor storability limits its economic potential as an income-generating venture for most women. This study was carried out to improve the shelf-life of pito through the addition of Moringa oleifera leaf extract and pasteurization (75 to 80°C). Microbial...

Author(s): Florence Adwoa Ayirezang, Courage Kosi Setsoafia Saba, Francis Kweku Amagloh and Hellie Gonu

November 2016

Characterization of mutant cowpea [Vigna unguiculata (L) Walp] lines using random amplified polymorphic DNAs (RAPDs) and amplified fragment length polymorphism (AFLP) markers

Phylogenetic relationship and polymorphism was detected in 10 cowpea lines comprising of leaf, flower and stem mutants, their putative parents and an exotic accession using 10 random amplified polymorphic DNAs (RAPDs) and three primer combinations of amplified fragment length polymorphism (AFLP) markers. These mutants were earlier obtained through the probable activities of transposable elements. The RAPD and AFLP...

Author(s): Olufisayo Atinuke Kolade, Mercy Oluremi Olowolafe and Iyiola Fawole

November 2016

Pan-genome analysis of Senegalese and Gambian strains of Bacillus anthracis

Bacillus anthracis is the causative agent of anthrax, and it is classified as “category A” biological weapon. There were six available complete genomes (A0248, Ames, Ames Ancestor, CDC684, H0491 and Sterne). Here, one Gambian and two Senegalese strains (Gmb1, Sen2Col2 and Sen3) were added. In this work, the pan-genome of B. anthracis was studied based on nine strains and using bioinformatics tools as Cluster...

Author(s): M. Mbengue, F. T. Lo, A. A. Diallo,  Y. S. Ndiaye, M. Diouf and M. Ndiaye

November 2016

RNAi-based silencing of genes encoding the vacuolar-ATPase subunits a and c in pink bollworm (Pectinophora gossypiella)

RNA interference is a post- transcriptional gene regulation mechanism that is predominantly found in eukaryotic organisms. RNAi demonstrated a successful gene silencing in insects that led to the development of novel approaches for insect pest management. In the current study, genes encoding vacuolar ATPase (V-ATPase) subunits a and c from the midgut of pink bollworm, Pectinophora gossypiella, were cloned and sequenced....

Author(s): Ahmed M. A. Mohammed

November 2016

Genetic analysis of body weight of Takifugu rubripes at different developmental stages

To elucidate the genetic mechanism of growth trait in Takifugu rubripes during ontogeny, developmental genetic analysis of body weight was conducted by mixed genetic model with additive-dominance effects, using complete diallel cross with three different strains of T. rubripes from Laizhou Shandong, Tangshan Hebei and Dalian Liaoning. Unconditional genetic analysis revealed that the unconditional dominance effects with...

Author(s): Aijun Ma,, and Xin’an Wang,,

November 2016

Diversity of growth hormone gene and its relation with average daily gain in Simmental cattle in West Sumatera Province, Indonesia

This study was aimed to analyse the genetic polymorphism of Growth Hormone (GH) polymorphism of Simmental cattle using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and its relation to average daily gain. The research was conducted in the Padang Mangatas Breeding Centre, Limapuluh Kota district, West Sumatera Province and Biotechnology Laboratory of Faculty of Animal Husbandry,...

Author(s): Hary Suhada, Sarbaini Anwar, Arnim, Hendri Maulana and Dan Yurnalis

November 2016

Hesperidin effects on behavior and locomotor activity of diabetic Wistar rat

Today, there are sufficient validated scientific data that support the existence of relations between diabetes and certain neuropsychiatric disorders, such as behavioral disorders, anxiety, cognitive decline and depression. The objective of this work was to investigate the effect of a natural bioflavonoid, the antioxidant hesperidin on the neuro behavioral alterations and locomotor function in streptozotocine diabetic...

Author(s): Doria Amina Bensaoula, Nadia Boukhris and Abdelkrim Tahraoui

November 2016

Optimization of humidifying procedure in controlled environment for indoor cultivation of Pleurotus pulmonarius

This study investigated mushroom cultivation in controlled indoor environment. For this, the environmental factors including humidity and temperature were controlled by humidifier and ventilation.  Four ventilations were installed on tops to bring the air from inside to outside and four small ventilations were installed on side wall along with black filter to bring the air from outside to inside. Four humidifiers...

Author(s): Tariqul Islam, Zarina Zakaria, Nasrul Hamidin and Mohd Azlan Bin Mohd Ishak

November 2016

Molecular epidemiology of foot and mouth disease, bluetongue and pest de petites ruminants in Algeria: Historical perspective, diagnosis and control

Molecular tools have become an increasingly important part of studying the epidemiology of infectious agents. These tools have allowed the aetiological agent within a population to be diagnosed rapidly with a greater degree of efficiency and accuracy than conventional diagnostic tools. They have enhanced understanding into the pathogenicity and virulence of the aetiological agent and subsequent deployment of appropriate...

Author(s): Moustafa KARDJADJ and Pam Dachung LUKA

November 2016

Chlorophyll, nitrogen and antioxidant activities in Cumaru (Dipteryx odorata (Aubl.) Willd) (Fabaceae) in two water regimes

The Cumaru (Dipteryx odorata (Aubl.) Willd.) is a species used by traditional populations and industries using timber and non-timber forest products. This study aimed to analyze the levels of chlorophyll A, B, total ammonia levels, nitrate, proline, electrolyte leakage and activity of oxidative enzymes in evaluation to tolerance of cumaru plants subjected to drought for 21 days of stress. The experiment was conducted in...

Author(s): Bruno Moitinho Maltarolo, Ellen Gleyce da Silva Lima, Vitor Resende do Nascimento, Kerolém Prícila Sousa Cardoso, Ana Ecídia de Araújo Brito, Tamires Borges de Oliveira, Glauco André dos Santos Nogueira, Karollyne Renata Souza Silva, Wander Luiz da Silva Ataíde, Cândido Ferreira de Oliveira Neto, Waldemar Viana De Andrade Junior and Benedito Gomes dos Santos Filho

November 2016

Enhancement of yield and nutritional value of soybean sprouts by persimmon fruit powder

Large amount of soybean sprouts has been produced for human consumption in Korea for a long time. Various techniques and production methods have been implied to enhance the quality and yield of soybean sprouts. The objective of the present study was to investigate the effect of persimmon fruit powder treatment on yield and nutritional value of soybean sprouts. The sprout yield was increased by 16% with the addition of...

Author(s): Il-Doo Kim, Sanjeev Kumar Dhungana, Jeong-Ho Kim, Hong Ahn, Hye-Ryun Kim and Dong-Hyun Shin

November 2016

In vitro direct organogenesis in response to floral reversion in lily

Our previous study indicated that the tiger lily (Lilium lancifolium var. Flore Pleno) has a great ability to produce inflorescence bulbils in nature as a form of natural phenomenon of floral reversion in plants. This present research was carried out to investigate the artificial floral reversion in in vitro culture of two lilies (Asiatic hybrid cv.”Black out"), and (Lilium longiflorum cv "White...

Author(s): Asker H. M.

November 2016

Genetic diversity and population structure of maize landraces from Côte d’Ivoire

Maize (Zea mays L.) occupies an important place among food crops in Côte d’Ivoire. However, no study on the genetic diversity of the species has been performed to date. This study aims at analyzing the diversity and genetic structure of 35 maize accessions using 10 microsatellite markers. These accessions are from different agro-ecological zones representative of the Ivorian territory. The results showed...

Author(s): N’DA Hugues Annicet, AKANVOU Louise, POKOU N’da Désiré, AKANZA Kouadjo Paul, KOUAKOU Charles Konan and ZORO BI Irié Arsène

November 2016

Molecular genetic diversity in a core of cocoa (Theobroma cacao L.) clones with potential for selection of disease resistance, plant height and fruit production

This study aimed to assess the genetic variability in groups of 11 clones of Theobroma cacao L., from different geographical regions, based on microsatellite markers, with the interest to characterize germplasm for breeding. The products of the amplification of these materials with 15 simple sequence repeat (SSR) markers were separated into ABI377 sequencer. The genotype encoded data were analyzed by means of...

Author(s): Adeilson Batista Lins, Pabliane Ramos Lawinscky, Antônia Marlene Magalhães Barbosa, Fernanda Amato Gaiotto and Ronan Xavier Corrêa

November 2016

Agronomic evaluation and quality characteristics of three Cucurbitaceae varieties acclimated in Benin

The present study aimed to evaluate some agronomical, physical and nutritional characteristics of seeds of three species of Cucurbitaceous, namely Citrulus lanatus, Lagenaria siceraria and Cucumeropsis edulus. Agronomic experiments were investigated over a two-year period during which, some agronomic performances of the tested varieties were evaluated. The physical characteristics of the collected fruits were also...

Author(s): Adam Salifou, Cherif Alidou, Fidèle P. Tchobo, Bruno Barea and Mohamed M. Soumanou

October 2016

Effect of salt stress on growth and contents of organic and inorganic compounds in noni (Morinda citrifolia L.)

Salinity is one of the abiotic stresses that most affect agricultural production, especially in arid and semi-arid regions; however, among species there are large differences in salt tolerance. In this study, the effects of salinity on the growth and accumulation of organic and inorganic solutes were evaluated in ‘noni’ seedlings at 1, 10, 20, 30 and 40 days of salt stress in a 5 x 2 completely randomized...

Author(s): Alide Mitsue Watanabe Cova, André Dias de Azevedo Neto, Rogério Ferreira Ribas, Hans Raj Gheyi and Renata Velasques Menezes

October 2016

Effect of nitrogen and potassium fertilization on morpho-agronomic traits of three elephant grass (Pennisetum purpureum Schum.) genotypes for biomass production

Elephant grass has been proposed for the energy sector as a possible source of renewable energy, because of its high biomass production. The aim of this study was to evaluate the effect of the mineral nutrients nitrogen and potassium on the morpho-agronomic traits (dry mater yield (DMY), percentage of DM (%DM), number of tillers per linear meter (NT), plant height (PH), stem diameter (SD) and leaf blade width (LW)) in...

Author(s): Antonio Alonso Cecon Novo, Rogério Figueiredo Daher, Geraldo de Amaral Gravina, Ernany Santos Costa, Juares Ogliari, Kleberson Cordeiro Araújo, Bruna Rafaela da Silva Menezes, Niraldo José Ponciano, Érik da Silva Oliveira and Verônica Britos Silva

October 2016

Study of associative effects of date palm leaves mixed with Aristida pungens and Astragalus gombiformis on the aptitudes of ruminal microbiota in small ruminants

The objectives of this trial were to evaluate interactions between microbial degradation of three substrates: date palm leaves as tannin-rich substrate, Astragalus gombiformis (as nitrogen source) and Aristida pungens (as carbon source). The model included the principal effects of single substrate and mixtures of multiple interactions. The forages were fermented alone or mixed with date palm leaves in various...

Author(s): Deffairi Djamila and Arhab Rabah

October 2016

Isolation and characterization of cellulolytic Bacillus licheniformis from compost

Eight cellulose degrading bacteria were isolated from compost and were identified as Bacillus licheniformis by 16S rRNA sequencing. Among the eight isolates, Bacillus licheniformis B4, B7 and B8 showed the highest cellulase activity. B. licheniformis B4 and B8 showed the maximum cellulase activity during the stationary phase of growth; but for B7, the maximum activity of cellulase was observed during the log phase....

Author(s): Nallusamy Sivakumar, Amira Al Zadjali, Saif Al Bahry, Abdulkhadir Elshafie and Elsadig Abdulla Eltayeb

October 2016

Drought tolerant tropical maize (Zea mays L.) developed through genetic transformation with isopentenyltransferase gene

Maize is a staple food crop for millions of Africans. Despite this fact, African farmers have been harvesting average grain yield of not more than 2 t/ha while there is a potential of producing more than 10 t/ha. Drought is one of the major abiotic constraints contributing to this low productivity. Drought diminishes crop productivity mainly by causing premature leaf senescence. The ipt gene codes for...

Author(s): Leta Tulu Bedada, Miccah Songelael Seth, Steven Maina Runo, Wondyifraw Teffera, Charless Mugoya, Clet Wandui Masiga, Richard Okoth Oduor, Eduardo Blumewald and Francis Wachira

October 2016

Transcriptional modulation of genes encoding nitrate reductase in maize (Zea mays) grown under aluminum toxicity

The free aluminum (Al) content in soil can reach levels that are toxic to plants, and this has frequently limited increased productivity of cultures. Four genes encoding nitrate reductase (NR) were identified, named ZmNR1–4. With the aim of evaluating NR activity and the transcriptional modulation of the ZmNR1, ZmNR2, ZmNR3, and ZmNR4 genes in leaves, 30-day-old hybrid maize BRAS 3010 plants were irrigated with a...

Author(s): Talita Cantú, Crislaine Emidio Vieira, Rafaélla David Piffer, Giovanna Carneiro Luiz and Silvia Graciele Hülse de Souza

October 2016

Alternative tube caps on in vitro growth of Orbignya oleifera Burret.: An Arecaceae native cerrado domain

The babassu (Orbignya oleifera Burret) is a palm that contains oilseeds whose biomass can be used for biofuel production. Due to difficulties in germinating the species, in vitro cultivation techniques are sought as a viable alternative to expand studies on germination. The objective of this study was to evaluate different types of tube caps for in vitro growth of babassu seedlings. Thus, zygotic embryos were inoculated...

Author(s): Mariluza Silva LEITE, Paula Sperotto Alberto FARIA, Flávia Dionísio PEREIRA, Fabiano Guimarães SILVA, Aurélio Rúbio NETO and João das Graças SANTANA

October 2016

On-line methanol sensor system development for recombinant human serum albumin production by Pichia pastoris

An on-line methanol sensor system was developed using a methanol probe, methanol sensor unit and peristaltic pump. The system was commanded using data acquisition (DAQ) and LabVIEW software. Calibration of the methanol sensor system was done in a medium environment with yeast cells during cells adaptation to methanol metabolism after glycerol feeding was stopped. The correlation equations between voltage output signal...

Author(s): Panchiga Chongchittapiban, Jӧrgen Borg, Yaowapha Waiprib, Jindarat Pimsamarn and Anan Tongta

October 2016

Cloning and expression analysis of alcohol dehydrogenase (Adh) hybrid promoter isolated from Zea mays

Hybrid promoters are created by shuffling of DNA fragments while keeping intact regulatory regions crucial of promoter activity. Two fragments of alcohol dehydrogenase (Adh) promoter from Zea mays were selected to generate hybrid promoter. Sequence analysis of both alcohol dehydrogenase promoter fragments through bioinformatics tools identified several crucial cis regulatory elements and transcription factors binding...

Author(s): Ammara Masood, Nadia Iqbal, Hira Mubeen, Rubab Zahra Naqvi, Asia Khatoon and Aftab Bashir

October 2016

Production and characterization of endoglucanase secreted by Streptomyces capoamus isolated from Caatinga

Cellulases are hydrolases of great importance to industries, especially due to their ability to produce ethanol via hydrolysis of cellulolytic materials. Actinomycetes are the producers of these enzymes, particularly the genus Streptomyces sp. The present study is the first report on the production and characterization of cellulolytic complex secreted by Streptomyces capoamus, isolated from the rhizosphere soil of...

Author(s): Rafael Lopes e Oliveira, Camila Beatriz Atanásio Borba, Sergio Duvoisin Junior, Patricia Melchionna Albuquerque, Gláucia Manoella de Souza Lima, Norma Buarque de Gusmão, Edmar Vaz de Andrade and Leonor Alves de Oliveira da Silva,

October 2016

SIM2PeD– Intelligent monitoring system for prevention of diabetic foot

Diabetes is an endocrine chronic disease that causes high blood sugar level, produced by retardation in, or deficiency of, glucose metabolism in the body of the individual with the disease. Neuropathies and/or angiopathies are complications of diabetes that result in changes in the lower limbs which subsequently evolve for the diabetic foot. Diabetic foot represents one of the most devastating complications of diabetes...

Author(s): Rodrigo Medeiros, Cicília Raquel Maia Leite, Suélia de Siqueira Rodrigues Fleury Rosa, Marina Pinheiro,  Luísiane de Ávila Santana and Jane Dulius

October 2016

Lipase production from a wild (LPF-5) and a mutant (HN1) strain of Aspergillus niger

In this study, a wild (LPF-5) and a mutant (HN1) strain of A. niger were compared for lipase production. Several physical parameters (carbon source, nitrogen source, pH, temperature and incubation period) were optimized for maximization of lipase production. Lipase activity between wild type and mutant strain were compared. Among all carbon sources, mixture of glucose (1%, w/v) and olive oil (1%, v/v) exhibited maximum...

Author(s): Arun Kumar Sharma, Vinay Sharma, Jyoti Saxena and Arindam Kuila

October 2016

Comparison of protease production from newly isolated bacterial strains T5, T3 and H3 after immobilization

Protease has gained a very important position in many industries such as food, pharmaceutical, chemical and leather industries. In this research, protease was obtained from bacteria. The bacterial strain was obtained from soil which was collected from different areas of Lahore, Pakistan. Fermentation medium (by using sub-merged fermentation technique) was incubated for 48 h at 37°C temperature and agitation speed of...

Author(s): Mina Ilyas

October 2016

DNA markers reveal genetic structure and localized diversity of Ethiopian sorghum landraces

North Eastern Ethiopia is a major sorghum-growing region. A total of 415 sorghum landraces were sampled to represent the range of agro-ecologies (three altitude ranges) as well as spatial heterogeneity, that is, 4 zones: North Welo, South Welo, Oromiya and North Shewa with each zone containing 2 to 5 districts. The landraces were genotyped with simple sequence repeats (SSR) and inter simple sequence repeats (ISSR)...

Author(s): Haile Desmae, David R. Jordan and Ian D. Godwin,

October 2016

Molecular screening of rice (Oryza sativa L.) germplasm for Xa4, xa5 and Xa21 bacterial leaf blight (BLB) resistant genes using linked marker approach

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has now become one of major rice diseases. During recent years, its occurrence has increased in Pakistan, particularly in ‘Kallar tract’ which is well-known for quality rice cultivation. Development of new resistant varieties by utilizing the available resistant gene-pool is most reliable breeding technique for the plant breeders. The...

Author(s): Muhammad Sabar, Tahira Bibi, Hafiz Umar Farooq, Zulqarnain Haider, Imad Naseem, Abid Mahmood and Muhammad Akhter

October 2016

Drying kinetics of Baru almond (Dipteryx alata Vog) used for the production of biodiesel

This study aimed to evaluate the drying kinetics of Baru (Dipteryx alata Vog), fit different mathematical models to the experimental values as a function of the water content, determine the diffusion coefficient and energy of Baru almonds during the drying process. Baru almonds were dried at 50, 60 and 70°C temperatures in a tray dryer until the water content reached 13% b.u. A non-linear regression analysis was...

Author(s): Paulo Cleber Mendonça Teixeira and Abraham Damian Giraldo Zuniga

October 2016

Renal and hepatic histopathology of intraperitoneally administered potassium permanganate on Clarias gariepinus juveniles

The biochemistry and histopathology of intraperitoneally administered potassium permanganate was investigated in Clarias gariepinus. Acute toxicity of the KMnO4 was determined by intraperitoneally injecting the fish with 0.0, 1.5, 2.0, 4.0 and 6.0 mg/kg. The 96 h lethal concentration (LC50) value obtained from the intraperitoneal injection of the juveniles of C. gariepinus with KMnO­4 was 2.001 mg/kg KMnO4 at 95%...

Author(s): Odo Gregory Ejikeme, Agwu Ekenma Juliana, Eke Felicia and Ikele Chika Bright and Ezea Celestine Osita

October 2016

Development of ethanol production from cooking oil glycerol waste by mutant Enterobacter aerogenes

Bacterial strains, capable of producing ethanol by using cooking oil glycerol waste (derived from biodiesel production) as sole C-source were screened from soil samples. Based on 16S rRNA sequence, the selected strain was identified as Enterobacter aerogenes G2WG. The sequence was submitted to GenBank and the accession number KU315428 was obtained. The genetic nature of the wild type strain was altered for enhancing...

Author(s): Thalisa Yuwa-amornpitak and Kannika Chookietwatana

October 2016

Production, characterisation and flocculation mechanism of bioflocculant TMT-1 from marine Bacillus pumilus JX860616

Bioflocculant from marine Bacillus pumilus JX860616 was characterised and its flocculation mechanism determined. The bacterium was identified by 16S rRNA and the bioflocculant was obtained through solvent extraction after optimum medium composition and culture conditions were established. The physicochemical analysis of the bioflocculant were obtained by scanning electron microscopic (SEM) equipped with elemental...

Author(s): Maliehe, T. S., Simonis, J., Basson, A. K., Reve, M., Ngema, S. and Xaba, P. S.

October 2016

In vitro regeneration of disease free enset [Ensete ventricosum (Welw) Cheesman] planting materials from bacterial wilt diseased plants using shoot tip culture

Enset is an important food crop produced in Ethiopia with great role in food security. The demand of the crop is increasing throughout the country. However, the production as a whole is decreasing due to devastation by enset bacterial wilt. The studies were conducted to develop the procedure for obtaining multiple disease free plantlets from infected enset plants. Shoot tip explants from infected suckers of three clones...

Author(s): Genene Gezahegn and Firew Mekbib

October 2016

Screening of spontaneous castor bean accesses for genetic improvement programs

The objective of this work was to identify, through the use of multivariate analysis, the spontaneous accesses of castor bean that show promising characteristics for inclusion in genetic improvement programs of this culture in Brazil. The study was conducted at the Paulista State University, Jaboticabal Campus. The accesses of castor bean seeds obtained were identified as ACS-001-CRSP, ACS-001-JASP, ACS-001-MASP,...

Author(s): Rener Luciano de Souza Ferraz, Jane Lima Batista, Marcelo de Andrade Barbosa, Ivomberg Dourado Magalhães, Alberto Soares de Melo and Antonio Sergio Ferraudo

October 2016

Assessment of the genetic diversity of Kenyan coconut germplasm using simple sequence repeat (SSR) markers

Genetic diversity and relationship among 48 coconut individuals (Cocos nucifera L.) collections from the Coastal lowland of Kenya were analyzed using 15 simple sequence repeat (SSR) primer pairs. Diversity parameters were calculated using Popgene Software version 1.31. The gene diversity values ranged from 0.0408 (CAC68) to 0.4861 (CAC23) with a mean value of 0.2839. The polymorphic information content (PIC) values...

Author(s): Maurice E. Oyoo, Najya Muhammed, Kimani N. Cyrus and Simon M. Githiri

October 2016

Evaluation of various pesticides-degrading pure bacterial cultures isolated from pesticide-contaminated soils in Ecuador

Due to the intensive use of pesticides within the greenhouse-rose production, remediation of polluted soils has become a hot topic for researchers in recent decades. Several bacterial strains having the ability to utilize various pesticides as a sole source of carbon and energy were isolated from pesticide-contaminated soils of Urcuquí, Ecuador. According to phenotypical, physiological, and biochemical...

Author(s): Pérez Mónica, Rueda O. Darwin, Bangeppagari Manjunatha, Johana J. Zúñiga, Ríos Diego, Rueda B. Bryan, Sikandar I. Mulla and Naga R. Maddela

October 2016

Use of response surface methodology to optimize the drying conditions of a bioactive ingredient derived from the African opaque sorghum beer

The present work aims to optimize the heat drying conditions of a bioactive feed ingredient derived from the African opaque sorghum beer. The bioactive ingredient was dried at various temperatures 35 to 50°C and times 5 to 24 h. The effects of the drying conditions on the dry matter, water activity, pH, titratable acidity, bacteriocin, ethanol, lactic acid, yeasts, lactic acid bacteria contents and antimicrobial...

Author(s): I. B. Chabi, A. P. P. Kayodé, B. P. Agbobatinkpo, A. Adénilé, O. A. Mamadou, G. Vieira-Dalodé, L. Baba-Moussa and J. T. C. Codjia

October 2016

Enzymatic regulation of organic acid metabolism in an alkali-tolerant halophyte Chloris virgata during response to salt and alkali stresses

Chloris virgata, an alkali-tolerant halophyte, was chosen as the test material for our research. The seedlings of C. virgata were treated with varying salt and alkali stress. First, the composition and content of organic acids in shoots were analyzed and the results indicated that there was not only a significant increase in total organic acids, but there were also obvious changes in different components of organic...

Author(s): Huan Wang, Bing Bai, Ziyu Bai, Lijun Shi, Jinlin Ye, Siwen Fan, Huahua Xu and Decheng Shi

October 2016

Comparison of four nonlinear growth models for effective exploration of growth characteristics of turbot Scophthalmus maximus fish strain

This study was conducted to compare the effectiveness for non-linear growth models designated as Chapman-Richards, Gompertz, Logistic and von Bertalanffy for selection of fast-growing fish strain of turbot Scophthalmus maximus. These models were compared using the goodness of fit (the coefficient of determination (R2) and the mean square error (MSE)) and the Akaike information criterion (AIC) and the growth...

Author(s): Xin’an Wang,, and Aijun Ma,,

October 2016

Genetic determinants of antifungal resistance in Candida species

In the previous decades, it has been an increase in cases of resistance to antifungal agents used in the prophylaxis and treatment of infections caused by Candida species. The emergence of resistance to drug classes, it is usually explained by genome alterations ranging from point mutations to gain or loss of whole chromosomes. Therefore, identify and understand into the molecular mechanisms that underlie resistance of...

Author(s): Danielly Beraldo dos Santos Silva, Alexéia Barufatti Grisolia and Kelly Mari Pires de Oliveira

October 2016

Phylogenetic relationships of the genus Quercus L. (Fageceae) from three different sections

In this study, the genetic diversity of 6 oak species known as Quercus coccifera L., Q. robur L., Q. infectoria Oliver, Q. cerris L., Q. ithaburensis subsp. macrolepsis (Kotschy) Hedge and Yalt. and Q. trojana P.B. Webb  in 18 populations was screened using the randomly amplified polymorphic DNA method (RAPD).  10 RAPD primers giving the best results produced 262 total loci. The highest and lowest band sizes...

Author(s): Yılmaz Aykut

October 2016

Antioxidant assessment on promethazinr HCl decomposition using RP-HPLC assay method

The objective of this study was to investigate the effect of different sodium metabisulfite (SMBS) concentrations under a variety of ICH recommended test conditions. An attempt was made to test the feasibility of increasing shelf life when stored under different conditions. The promethazine hydrochloride (HCL) sample solutions used according to USP 24 and BP 1999 were prepared using different concentration of sodium...

Author(s): Rasha Saad, Heyam Saad Ali, Babiker M. A. Elhaj and Mai Al Ajaji

September 2016

Molecular identification of Anopheles gambiae sensu stricto Giles (formerly Anopheles gambiae Savannah Form) in Kamuli District, Uganda

Anopheles gambiae sensu stricto Giles (formerly A. gambiae S molecular form), the largely anthropophilic species, is reportedly the most important malaria vector in Uganda among the A. gambiae complex species. Indoor and outdoor human-biting mosquitoes were caught for four consecutive nights in each of 48 households in Kamuli district using human-baited bed net traps for subsequent identification of the principal...

Author(s): Fredrick George Kabbale, Anne Margaret Akol, John Baptist Kaddu, Enock Matovu, Anne Kazibwe, Anges Yadouleton and Ambrose Winston Onapa

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