African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

AJB Articles

September 2016

Perennial soybean seeds coated with high doses of boron and zinc

The objective of this work was to study combinations of high doses of boron (B) and zinc (Zn) in the recoating of perennial soybean seeds, in order to provide these nutrients to the future plants. The physical, physiological and nutritional characteristics of the coated seeds and initial development of plants in a greenhouse were evaluated. Tests carried out in the laboratory were conducted in a completely randomized...

Author(s): Amanda Justino Acha, Henrique Duarte Vieira and Marta Simone Mendonça Freitas

September 2016

Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene

Jatropha curcas L. oil has been shown to be suitable for the production of biodiesel. However, this species has not been domesticated yet. Genetic breeding through conventional methods is time consuming and costly, hence, genetic transformation could contribute positively to the improvement of interesting traits. Although in vitro regeneration and stable genetic transformation has been pursued for several years,...

Author(s): Mariana Crotti Franco, Kleber Alves Gomes, Marcel Mamede de Carvalho Filho, Ricardo Harakava, Nicolas Carels, Walter José Siqueira, Rodrigo Rocha Latado and Daniela de Argollo Marques

September 2016

Germination, growth and physiological responses of Senegalia senegal (L.) Britton, Vachellia seyal (Delile) P. Hurter and Prosopis juliflora (Swartz) DC to salinity stress in greenhouse conditions

Salinity is among the most widespread environmental threats to global plant production, especially in arid and semi-arid climates. Thus, the selection of salt tolerant species is necessary for sustainable plant productivity. The purpose of this study was to measure and understand the salt tolerance of three multipurpose trees used in reforestation programs in many Sahelian countries (Senegalia senegal, Syn. Acacia...

Author(s): Dioumacor Fall,, Niokhor Bakhoum, Fatoumata Fall,, Fatou Diouf, Mamadou O. Ly, Mayécor Diouf, Djamel Gully, Valérie Hocher and Diégane Diouf,

September 2016

Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis × Elaeis oleifera)

Oil palm hybrid BRS Manicoré is important for plantations in the north of Brazil, as it is resistant to fatal yellowing and is compact. Seed germination is slow and reduced, so somatic embryogenesis is a promising alternative for its propagation. Two kinds of starting explants were used: Zygotic embryos (ZE) and thin cell layers (TCL) from the base of seedling aerial parts. Two culture medium formulae were...

Author(s): Bonetti, K. A. P., Nesi, J., Quisen, R. C. and Quoirin, M.

September 2016

Genetic diversity of rice (Oryza sativa) germplasm from six countries using simple sequence repeats markers

This experiment was carried out to determine the genotypic variation among rice (Oryza sativa) accessions using simple sequence repeats (SSR) markers. In the present study, a total of 12 SSR markers were used across 87 rice accessions from six countries. NTSYS-pc and PowerMarker software were used for data analysis. Six primers out of these 12 primers showed DNA amplification and polymorphism among the 87 rice...

Author(s): Ibrahim Soe, Richard Akromah, Daniel Nyadanu, Maxwell Darko Asante and Alex Tamu

September 2016

The rolling circle amplification and next generation sequencing approaches reveal genome wide diversity of Kenyan cassava mosaic geminivirus

Rolling circle amplification is a simple approach of enriching populations of single-stranded DNA plant begomovirus genomes (genus, Begomovirus; family, Geminiviridae). This is an innovative approach that utilizes the robustness of the bacteriophage phi29 DNA polymerase used in circle amplification, together with deep sequencing using Illumina Miseq and bioinformatics to assess population diversity of begomoviruses in...

Author(s): T. M. Kathurima, , E. M. Ateka, A. B. Nyende and T. A. Holton

September 2016

Influence of recirculation rate on the performance of a combined anaerobic-aerobic reactor applied to the removal of carbon and nitrogen from poultry slaughterhouse wastewater

The objective of this study was to evaluate a combined anaerobic-aerobic upflow fixed-bed reactor with liquid phase recirculation for the removal of nitrogen and organic matter from poultry slaughterhouse wastewater. The reactor performance was evaluated with a hydraulic retention time (HRT) of 11 h and three different recirculation rates (R=0.5; 1 and 2). The highest nitrogen removal efficiency value was obtained with...

Author(s): Carla L. Lopes, Adriana N. de Lima Model, Jackeline T. Gotardo, Fernando H. Passig, Eloy L. de Mello, Benedito M. Gomes and Simone D. Gomes

September 2016

Comparison between Giemsa and Van Geison stains in demonstration of collagen fibers (Kosti-2016)

Trichrome stain (such as Van Geison) is usually used in histopathology laboratory for demonstration of collagenic fibers. Lack of selectivity and tendency of stain to fade makes van Gieson not ideal for collagen demonstration. This study was aimed to compare between Giemsa's and van Gieson's stains in collagen fibers demonstration. Twenty biopsies were obtained from rabbit’s skin after anesthesia by...

Author(s): Ziyad Mudasir, Salma Adil and Ahmed Ibn Edriss

September 2016

Ex-vivo evaluation of crab shell chitosan as absorption enhancer in ciprofloxacin tablet formulation

This study was aimed at evaluating crab shell chitosan as absorption enhancer in ciprofloxacin tablet formulation using the ex-vivo model. Six batches of ciprofloxacin tablets containing varying concentrations of crab shell-derived chitosan ranging from 0 to 5% w/w at 1% w/w intervals were produced. Batch CTS-0 containing no chitosan served as the control. The crushing strength, friability, disintegration time,...

Author(s): Emmanuel O. Olorunsola, Timma O. Uwah, Olubunmi J. Olayemi and Unyime B. Etukudo

September 2016

Optimization of somatic embryogenesis procedure for commercial clones of Theobroma cacao L.

The first objective of this study was to assess and optimize somatic embryo production in a genetically diverse range of cacao genotypes. The primary and secondary somatic embryogenesis response of eight promising cacao clones and a positive control was evaluated using modified versions of standard protocols. The second objective was to optimize the efficiency of primary somatic embryogenesis for a commercially...

Author(s): Garcia Claudia, Corrêa Fabio, Findley Seth, Almeida Alex-Alan, Costa Marcio, Motamayor Juan Carlos, Schnell Ray and Marelli Jean-Philippe

September 2016

Physiological responses by Billbergia zebrina (Bromeliaceae) when grown under controlled microenvironmental conditions

Sucrose, the most commonly used carbon source in conventional in vitro culture, and limited air exchange in the culture containers are factors that affect the growth of in vitro-cultured plants. They may induce physiological disorders and decrease the survival rate of plants after transfer to ex vitro conditions. The aim of the present study was to analyze the effects of gas exchange and sucrose concentration on...

Author(s): João Paulo Rodrigues Martins, Veerle Verdoodt, Moacir Pasqual and Maurice De Proft

September 2016

Fruit production and quality of guava ‘Paluma’ as a function of humic substances and soil mulching

The uses of humic substances and organic residues for soil mulching have been more common during last years. This way, an experiment was carried out to evaluate the fruit quality and yield of guava cv. Paluma as a function of humic substances and organic soil mulching in Brazil. The experiment was performed from January 2012 to June 2013 (first trial) and from July to November 2013 (second trial) using a randomized...

Author(s): Leonardo Fonseca da Rocha, Lourival Ferreira Cavalcante, Járisson Cavalcante Nunes, Antônio Gustavo de Luna Souto, Alian Cássio Pereira Cavalcante, Ítalo Herbert Lucena Cavalcante and Walter Esfrain Pereira

September 2016

Growth and physiological aspects of bell pepper (Capsicum annuum) under saline stress and exogenous application of proline

This study aimed to evaluate growth and physiological aspects of ‘All Big’ bell pepper, under saline stress and exogenous application of proline on the leaves. The research was conducted in pots adapted as drainage lysimeters under greenhouse conditions, using sandy-loam eutrophic Regolithic Neosol, in the municipality of Campina Grande-PB, Brazil. The experiment was set in randomized blocks, in order to...

Author(s): Francisco Vanies da Silva Sá, Geovani Soares de Lima, João Batista dos Santos, Hans Raj Gheyi, Lauriane Almeida dos Anjos Soares, Lourival Ferreira Cavalcante, Emanoela Pereira de Paiva and Leandro de Pádua Souza

September 2016

Protective effects of some fruit juices with low-fat diet on rat testis damaged by carbon tetrachloride: A genetic and histological study

Oxidative stress, free radical, lipid peroxidation and antioxidant have become a common expression with most disease and methods for protection. Carbon tetrachloride (CCl4) is an industrial solvent which has destructive effects on a cell while most fruit juices have antioxidant effects. The aim of this study was to investigate the protective role of fruit juice on testis after toxic effect with CCl4 through oxidative...

Author(s): Medhat R. Rehan, Amina M. G. Zedan, Samah A. El-Hashash, Mona A. Farid and Gehan A. El-Shafie

September 2016

Confirmation of antibodies against L-tryptophan-like epitope in human African trypanosomosis serological diagnostic

Antibodies directed against L-tryptophan epitope (WE - W for tryptophan, E for epitope), a constant epitope borne by variant surface glycoproteins (VSG), have been detected in sera of all 152 Human African Trypanosomosis (HAT) patients from Angola. The WE is present in VSG hydrophobic regions of the C terminal domains. In the assay, L-tryptophan was linked to bovine serum albumin (BSA) with glutaraldehyde to synthesize...

Author(s): Silla Semballa,, Romaric Nzoumbou-Boko, , Philippe Holzmuller , Paul Chuchana , Jean-loup Lemesre, Philippe Vincendeau, Pierrette Courtois, Sylvie Daulouède and Philippe Truc,

September 2016

Evaluation of phenolic compounds of two Lygeum spartum L. cytotypes

Lygeum spartum represents a natural barrier in the Algerian high plateaus against the advance of sand and the desertification. The plant is of interest due to its tolerance to environmental stress. There are two levels of ploïdy in this species; the diploid cytotype 2n = 16 which is confined in the locality of Ain Benkhllil and the polyploid cytotype 2n = 40 which thrives in semi-arid climates and has a large...

Author(s): Louerrad Yasmina and Kaid-Harche Meriem

September 2016

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Micropropagation requires controlling contamination that might compromise the success of the process. Thermal sterilization is traditionally used; however, costs deriving from equipment acquisition and maintenance render this technique costly. With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for...

Author(s): Ana Karolina Pais, Ana Paula da Silva, Joselita Cardoso de Souza, Silvio Lopes Teixeira, Juliana Martins Ribeiro, Ana Rosa Peixoto and Cristiane Domingos da Paz

August 2016

Pharmacological properties of cashew (Anacardium occidentale)

Anacardium occidentale L. is a tree native to Brazil, which is rich in phenolic lipids. Nowadays, the cashew bark (Cashew Nut Shell Liquid) has received great attention in the pharmaceutical industry, due to its economy, abundance and important chemical compounds. Net of cashew nut shell is classified according to the method of production of: (1) net of the shell of natural cashew nut (60-65% anacardic acid; 15-20%...

Author(s): Aracelli de Sousa Leite,, Md. Torequl Islam,, Antonio Luiz Gomes Júnior,,  João Marcelo de Castro e Sousa, Marcus Vinícius Oliveira Barros de Alencar,, Márcia Fernanda Correia Jardim Paz, Hercília Maria Lins Rolim, Maria das Graças Freire de Medeiros, Ana Amélia de Carvalho Melo-Cavalcante, and José Arimatéia Dantas Lopes,

August 2016

Aflatoxins: A silent threat in developing countries

Several mycotoxins are known to contaminate crop produce and processed forms but aflatoxins are the most common. They are mainly produced by fungi belonging to the genera Aspergillus and Penicillium. Cereals and their products which constitute the staples in most developing countries are particularly vulnerable to attack by aflatoxigenic fungi. Despite the potential health risk posed to animals and humans, many people...

Author(s): Elias Nortaa Kunedeb Sowley

August 2016

Phylogenetic analysis of 23S rRNA gene sequences of some Rhizobium leguminosarum isolates and their tolerance to drought

The phylogenetic relationships among thirteen Rhizobium leguminosarum  bv. viciae isolates collected from various geographical regions were studied by analysis of the 23S rRNA sequences. The average of genetic distance among the studied isolates was very narrow (ranged from 0.00 to 0.04) and the studied isolates formed two main groups based on cluster analysis. The isolates were tested for their growth on YMA...

Author(s): Khalid S. Abdel-Lateif, Omar A. Hewedy and Abdel Fattah M. El-Zanaty.

August 2016

Resistance of corn genotypes to fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

The objective of this study was to evaluate resistance mechanisms in 12 corn genotypes (transgenic hybrids: 30A91 PW, 20A78 HX, Impacto VIP 3, 20A55 HX, NS90 PRO 2, Maximus VIP 3, BX 1293YG, RB 9004 PRO, Feroz VIP 3, LG 6036 PRO; conventional: AG 1051 and variety: AL Bandeitante). Attractiveness, aversion to feeding, and antibiosis were evaluated via free and no choice tests in the laboratory. Attractiveness was...

Author(s): Lígia Alves de Paiva, Fernanda Corrêa, Cinthia Luzia Teixeira Silva, Tony de Lima Moura, Franciele Cristina da Silva, Marcio da Silva Araújo and Flávio Gonçalves de Jesus

August 2016

Genetic diversity assessment of farmers’ and improved potato (Solanum tuberosum) cultivars from Eritrea using simple sequence repeat (SSR) markers

Sixty three potato clones (51 farmers’ and 12 varieties) from Eritrea, 18 and 12 varieties from Kenya and Rwanda, respectively were characterized using 12 highly polymorphic simple sequence repeat (SSR) markers. The study was designed to assess the genetic diversity and varietal distinctness among the different samples. In total, 91 alleles ranging between 2 (STM1053) to 13 (STM0031) alleles per marker were...

Author(s): Biniam Mesfin Ghebreslassie, S. Mwangi Githiri, Tadesse Mehari, Remmy W. Kasili, Marc Ghislain  and Eric Magembe

August 2016

Efficiency and response of conilon coffee genotypes to nitrogen supply

The objective of the study was to differentiate genotypes with higher efficiency and responsiveness to nitrogen supply, to understand how the nitrogen supply can impact the dry matter allocation and the accumulation of this nutrient in the different plant compartments of genotypes of conilon coffee, cultivated under contrasting conditions of nitrogen availability in the soil. The plants were cultivated during 150 days...

Author(s): Lindomar Souza Machado, Lima Deleon Martins, Wagner Nunes Rodrigues, Daniel S. Ferreira, Adan Dezan Côgo, Marcelo Antônio Tomaz and José Francisco Teixeira do Amaral

August 2016

Phosphate solubilization and multiple plant growth promoting properties of rhizobacteria isolated from chickpea (Cicer aeritinum L.) producing areas of Ethiopia

Chickpea is one of the major legume crops widely grown in Ethiopia. The low availability of phosphorus in soil is among the stresses that constrain the production of this crop in the country. However, there are rhizobacteria capable of solubilizing insoluble forms of phosphorus in soil and make it available to the plant. Thus, this study was aimed at isolation and characterization of phosphate solubilizing bacteria from...

Author(s): Mulissa J. Midekssa, Carolin R. Löscher, Ruth A. Schmitz and Fassil Assefa

August 2016

Effect of packaging and chemical treatment on storage life and physicochemical attributes of tomato (Lycopersicon esculentum Mill cv. Roma)

Fresh fruits and vegetables are inherently more liable to deterioration under tropical conditions characterized by high ambient temperatures and humidity. In determining the effects of chemical treatment on tomatoes (Lycopersicon esculentum Mill cv. Roma), fruits purchased at turning stage of ripening were packaged in low density polyethylene bags (60 µ) containing wooden dust particles moistened with 400 ppm...

Author(s): Tonna A. Anyasi, Charles O. Aworh and Afam I. O. Jideani

August 2016

Response of chickpea (Cicer arietinum L.) to inoculation with native and exotic Mesorhizobium strains in Southern Ethiopia

A series of pot and two consecutive crop-year field experiments were conducted from 2011 to 2012 in Southern Ethiopia to determine the effectiveness of Mesorhizobium strains on two cultivars of chickpea (Shasho and Nattoli). The eight treatments included: Six rhizobial inoculants, the four best indigenous strains (Cp8, Cp41, Cp97 and Cp105); CpNSTC (National Soils Testing Center inoculant); and CpSK (Canadian...

Author(s): Wondwosen Tena, Endalkachew Wolde-Meskel and Fran Walley

August 2016

Evaluation methods used for phosphate-solubilizing bacteria

Phosphorus solubilizers naturally acidify rhizospheric soil and increase phosphorus availability; therefore, their evaluation may help to reduce phosphorus fertilizer use. This work aimed to evaluate the different selection methods and select inorganic phosphorus-solubilizing bacteria as potential plant-growth promoters. Bacterial isolates obtained from sugarcane roots and soil were tested using solid growth media...

Author(s): Clayton Albuquerque Sousa, Mario de Andrade Lira Junior, Giselle Gomes Monteiro Fracetto, Fernando José Freire, and Júlia Kuklinsky Sobral

August 2016

Efficient micropropagation of Citrus sinensis (L.) Osbeck from cotyledonary explants suitable for the development of commercial variety

In vitro regeneration of sweet orange (Citrus sinensis (L.) Osbeck Family: Rutaceae) has been performed via direct and indirect organogenesis. For indirect organogenesis, callus was induced and proliferated from leaf explants derived from in vitro grown seedlings on Murashige and Skoog (MS) media containing 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with benzyl adenine (BA) and α-naphthalene...

Author(s): Aseesh Pandey and Sushma Tamta

August 2016

Production of extracellular laccase from the newly isolated Bacillus sp. PK4

Laccase belongs to the family of multicopper oxidase and have wide range of biotechnological applications starting from the food industry to bioremediation. However, the use of laccase at commercial scale is hindered by factors like high enzyme cost, low activity and /or stability under given conditions. This study was carried out with aim of screening for extracellular thermostable laccase producing bacteria....

Author(s): Murugesan Rajeswari and Vembu Bhuvaneswari

August 2016

Polyhydroxyalkanoate (PHA) production by Lynisibacillus sp. strain UEA-20.171

PHA is a biodegradable and biocompatible natural thermoplastic produced from renewable bioresources and is hence attracting attention as a plastic material for use in the environment and medical fields. In the present study, the Lynisibacillus sp. strain UEA-20.171 was selected for production of polyhydroxyalkanoate (PHA) in bioreactor. The accumulation of polymer in the bacterium occurs when there is an imbalance in...

Author(s): Edilane Martins Ferreira Aldo Rodrigues de Lima Procópio Raimundo Carlos Pereira Junior Sandra Patricia Zanotto Rudi Emerson de Lima Procópio

August 2016

Assessment of biofuel potential of dead neem leaves (Azadirachta indica) biomass in Maroua town, Cameroon

Dead leaves of neem trees in the Sahelian urban zone are among the wastes that are underutilized, since it is either buried or burnt, and thus, contribute to increased environmental pollution. Unfortunately, the lack of information on the biomass and energy potentials of these wastes empedes any initiative for its industrial biomethanization. This study was investigated with the aim of evaluating the biofuel potentials...

Author(s): Tizé Koda Joël, Sinbai Marcel, Darman Roger Djouldé and Albert Ngakou

August 2016

Genetic determinism of oil acidity among some DELI oil palm (Elaeis guineensis Jacq.) progenies

The oil palm (Elaeis guineensis Jacq.) is cultivated mainly for crude palm oil (CPO) which is extracted from the mesocarp of fruits. The quality of CPO is generally impaired due to high acidity, as a result of the activity of a lipase present in the mesocarp of the fruits at maturity. The objective of this study was to establish the genetic determinism of “palm oil acidity” (POA) from E. guineensis. Acidity...

Author(s): Benoit Constant LIKENG-LI-NGUE, Joseph Martin BELL, Georges Franck NGANDO-EBONGUE, Godswill Ntsefong NTSOMBOH and Hermine Bille NGALLE

August 2016

Functional properties of milk drinks flavored with mangaba pulp and enriched with passion fruit bark flour

Dairy foods including dairy based drinks play an important role in human nutrition. The flour of the passion fruit peel has high potential for use in the enrichment dairy drinks improving the nutritional and technological qualities besides an alternative to reduce waste by-products in the food industry. Thus, this study aimed to evaluate the physical and chemical parameters, texture, color, chemical composition,...

Author(s): Lígia Campos de Moura, Marco Antônio Pereira da Silva, Geovana Rocha Plácido, Márcio Caliari, Diene Gonçalves Souza, Jéssica Leal Freitas e Souza, Juliana Aparecida Célia, Kênia Borges de Oliveira, Karen Martins Leão and Luiz Eduardo Costa do Nascimento

August 2016

Organic and conventional vegetables: Comparison of the physical and chemical characteristics and antioxidant activity

The objective of this research was to compare the physical and chemical characteristics and antioxidant activity of organic and conventional carrot (Daucus carota), green pepper (Capsicum annuum) and lettuce (Lactuca sativa). Five representative samples of  conventional vegetables, certified organic and non-certified organic vegetables were gotten from farms and supermarkets in the city of Rio de Janeiro. The...

Author(s): Fernanda de Oliveira Pereira, Renata dos Santos Pereira, Lana de Souza Rosa and Anderson Junger Teodoro

August 2016

Oxidative enzymes in coconut cultivars in response to Raoiella indica feeding

The increase in oxidative enzyme activities is related to diminished mite infestation. Some biological aspects of Raoiella indica Hirst reared on the coconut cultivars (‘Jamaican Tall’ (JT), ‘Malayan Yellow Dwarf’ (MYD), Niu Leka (NL) and a hybrid JT x MYD) were studied under laboratory conditions. Aditionally, changes in oxidative enzyme activities (peroxidase and polyphenol oxidase) as response...

Author(s): Carlos Vásquez, Marta Dávila, Naileth Méndez, María A. Jiménez and María F. Sandoval and Francisco J. Alcalá,

August 2016

Ovarian follicular dynamics in purebred and crossbred Boran cows in Ethiopia

Boran is an endangered breed of cattle indigenous to Ethiopia and the relatively poor understanding of its reproductive physiology has impeded efforts to maximize reproductive performance of the breed. This study characterized ovarian follicular dynamics in 9 purebred Boran and 8 Boran×Holstein (B×H) crossbred cows. Ovaries of all 17 cows were examined once per day for 61 consecutive days (encompassing three...

Author(s): Tamrat Degefa, Alemayehu Lemma, Jeilu Jemal, Gbremeskel Mamo, Azage Tegegne and Curtis R. Youngs

August 2016

Micropropagation of caçari under different nutritive culture media, antioxidants, and levels of agar and pH

The caçari (Myrciaria dubia) is a native fruit tree from Amazon with high concentrations of vitamin C. This study aimed to adjust a culture medium that meets the nutritional needs for the in vitro development of caçari, evaluating the effect of different concentrations and nutritive culture media, antioxidant, and levels of agar and pH. Three experiments were carried out in a completely randomized design:...

Author(s): Maria Da Conceicao Da Rocha Araujo, Edvan Alves Chagas, Maria Isabel Ribeiro Garcia, Sara Thiele Sobral Pinto, Pollyana Cardoso Chagas, Wagner Vendrame, Adamor Barbosa Mota Filho and Olisson Mesquita de Souza

August 2016

Plant growth-promotion by Streptomyces spp. in sorghum (Sorghum bicolor L.)

Seven strains of Streptomyces spp.: BCA-546 (KF770898), BCA-659 (KF770889), BCA-667 (KF770888), BCA-689 (KF770899), BCA-698 (KF770900), CAI-133 (KF770895) and CAI-8 (KF770890), reported earlier to produce biocontrol and plant growth-promoting (PGP) substances were further evaluated for PGP traits in sorghum under greenhouse and field conditions. Under greenhouse conditions, plant height, leaf area and weight, root...

Author(s): Gottumukkala Alekhya, and Subramaniam Gopalakrishnan

August 2016

Nutritional and antimicrobial evaluation of Saccharum officinarum consumed in Calabar, Nigeria

Sugarcane (Saccharum officinarum) stem pulp is widely consumed in Nigeria as a snack mainly for sugar content and is believed to possess some remedy against infectious diseases. This study therefore quantitatively evaluated the nutritional and chemical composition of the sugarcane stem pulp with a view to validating this claim. The results of proximate composition indicate relatively high amount of moisture (71.91...

Author(s): Ima Okon Williams, Eridiong Ogbonna Onyenweaku and Item Justin Atangwho

August 2016

Propagation of Gladiolus corms and cormels: A review

Gladiolus is an important estimated 8th in the world cut flower trade’s cut flower grown throughout the world for its elegant attractive spikes of different hues and good keeping quality. The commercial cultivation of Gladiolus is based on natural multiplication of corms and cormels. However, multiplication rate of corms and cormels is slow and the conventional method of propagation is insufficient to meet the...

Author(s): Noor-Un-Nisa Memon, Niaz Ahmad Wahocho, Tanveer Fatima Miano and Mujahid Hussain Leghari

August 2016

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Curcuma longa L., from the Zingiberaceae family, generally reproduces through its rhizomes, which are also utilized for therapeutic purposes because they are rich in terpenoids. Its conventional propagation has low efficiency due to the small number of seedlings and their contamination by pathogens. Therefore, this study aimed to evaluate the influence of growth regulators on the development of in vitro-cultivated C....

Author(s): Dirlane Antoniazzi, Meire Pereira de Souza Ferrari, Andressa Bezerra Nascimento, Flávia Aparecida Silveira, Leila Aparecida Salles Pio, Moacir Pasqual and  Hélida Mara Magalhães

August 2016

Micropropagation of Launaea cornuta - an important indigenous vegetable and medicinal plant

An efficient micropropagation protocol was developed for the medicinal plant Launaea cornuta using green house axillary buds as explants. The best sterility was obtained at 30% (v/v) local bleach (JIK). Maximum shoot induction rate was achieved when axillary buds were cultured on Murashige and Skoog (MS) Media supplemented with 0.5 mg/L of 6-benzylaminopurine (BAP) for 3 weeks. The highest number of shoot multiplication...

Author(s): Faith Ambajo and Jonathan Mutie Matheka

August 2016

Seed origin, storage conditions, and gibberellic acid on in vitro germination of Campomanesia adamantium (Cambess.) O. Berg

Campomanesia adamantium (Cambess.) O. Berg seeds (guavira) are recalcitrant and rapidly lose viability upon removal from the fruit, making difficult the long-term storage. In vitro germination could be used as an important tool to overcome the issues related to this short viability. It might help seed conservation and species propagation. The purpose of this study was to evaluate in vitro germination of guavira seeds...

Author(s): Cláudia Roberta Damiani, Leandro Darc da Silva, Ademir Goelzer and Thamiris Gatti Déo

August 2016

Proteomic variation in Korean ginseng (Panax ginseng C.A. Meyer) isolates from different geographic regions

Korean ginseng is a traditional medicine that is widely used in Korea. In this study, a proteomic approach was used to investigate variations in Korean ginseng isolates that are associated with ecologic and geographic differences. Ginseng samples were collected from four geographically isolated locations in Korea: North gyeonggi, Gochang, Geumsan and Kanghwa. Two-dimensional gel electrophoresis (2-DE) and peptide...

Author(s): KiBeom Lee and KwanSoon Park

August 2016

Assessment of feeding value of vegetable-carried pineapple fruit wastes to Red Sokoto goats in Ogbomoso, Oyo State of Nigeria

This study compared the sun-drying characteristics of five blends each (w/w; 1:1, 1:1.5, 1:2, 1:2.5, 1:3) of wheat offal-carried pineapple waste (WO:PW) and brewers’ dried grains-carried pineapple waste (BDG:PW), assessed the blends for their nutrient contents and the feeding value of the optimum blends with Red Sokoto (RS) goats. Moisture contents of all the blends were reduced to between 10.95 - 14.38% and 11.73...

Author(s): Vincent Olajide ASAOLU, Rachael Temitope BINUOMOTE and Oyeniyi Sunmiboye OYELAMI

August 2016

Isolation and characterization of two malathion-degrading Pseudomonas sp. in Egypt

Pseudomonas aeruginosa and Pseudomonas mendocina degrading malathion were studied. Morphological, biochemical and 16S rRNA genes for bacterial identification were selected. Biodegradation of some organophosphorus compounds with the 2 bacterial isolates was determined by high performance liquid chromatography (HPLC). P. aeruginosa strain completely removed diazinon, malathion and fenitrothion, but not chlorpyrifos within...

Author(s): Amal E. Saafan, Ahmed F. Azmy, Magdy A. Amin, Shabaan H. Ahmed and Tamer M. Essam

August 2016

Analysis of the impact of domestication of Warburgia ugandensis (Sprague) on its genetic diversity based on amplified fragment length polymorphism

Warburgia ugandensis Sprague (Canellaceae) occurs in East and Central Africa and is an important multipurpose tree species. Over-exploitation of natural forests for medicinal purposes and clearance for farming threaten the species survival. Cultivation of the tree species would ensure sustainable medicinal source and its conservation. However, on-farm genetic diversity of the species is currently unknown. The genetic...

Author(s): Nkatha Gacheri, Bramwel W Wanjala, Ramni Jamnadass and Alice Muchugi,

August 2016

Optimization, purification and characterization of recombinant L-asparaginase II in Escherichia coli

We studied optimal L-asparaginase sequence from GenBank accession number X12746 encoding for L-asparaginase from Erwinia chrysanthemi NCPPB1125. The expression level of recombinant L-asparaginase was determined as 78% of the total proteins. The purified L-asparaginase had a molecular mass of 37 kDa with specific activity of 312.8 U/mg. Kinetic parameters, Km, Vmax, Kcat and Kcat/Km of purified enzyme were found to be...

Author(s): Trang Thi Hien Nguyen, Cuong Tien Nguyen, Thanh Sy Le Nguyen and Tuyen Thi Do

August 2016

Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa

Banana Germplasm Collection serves as a source of useful genes for banana breeding. However, insufficient and/or inaccurate information on the ploidy level of the germplasm renders its utilization in breeding difficult. The objective of this study was to determine and validate the ploidy level of 120 banana accessions in the ex situ germplasm collection centre for the East and Central Africa, located in Mbarara, Uganda....

Author(s): Deborah Karamura, Robooni Tumuhimbise, Sedrach Muhangi, Moses Nyine, Micheal Pillay, Reuben Ssali Tendo, David Talengera, Priver Namanya, Jerome Kubiriba and Eldad Karamura

July 2016

Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut

Essential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based...

Author(s): Maria Isabel G. Martins, Antonio Euzébio G. Sant&#;Ana, Felipe Matheus T. Vasconcelos, Wbyratan L. Silva, Liziane M. Lima, Reginaldo Carvalho, Péricles A. Melo Filho and Roseane C. Santos

Page 14 of 245, showing 50 records out of 12227 total, starting on record 651, ending on 700