African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12268

AJB Articles

Study of associative effects of date palm leaves mixed with Aristida pungens and Astragalus gombiformis on the aptitudes of ruminal microbiota in small ruminants

October 2016

The objectives of this trial were to evaluate interactions between microbial degradation of three substrates: date palm leaves as tannin-rich substrate, Astragalus gombiformis (as nitrogen source) and Aristida pungens (as carbon source). The model included the principal effects of single substrate and mixtures of multiple interactions. The forages were fermented alone or mixed with date palm leaves in various...

Author(s): Deffairi Djamila and Arhab Rabah

Isolation and characterization of cellulolytic Bacillus licheniformis from compost

October 2016

Eight cellulose degrading bacteria were isolated from compost and were identified as Bacillus licheniformis by 16S rRNA sequencing. Among the eight isolates, Bacillus licheniformis B4, B7 and B8 showed the highest cellulase activity. B. licheniformis B4 and B8 showed the maximum cellulase activity during the stationary phase of growth; but for B7, the maximum activity of cellulase was observed during the log phase....

Author(s): Nallusamy Sivakumar, Amira Al Zadjali, Saif Al Bahry, Abdulkhadir Elshafie and Elsadig Abdulla Eltayeb

Drought tolerant tropical maize (Zea mays L.) developed through genetic transformation with isopentenyltransferase gene

October 2016

Maize is a staple food crop for millions of Africans. Despite this fact, African farmers have been harvesting average grain yield of not more than 2 t/ha while there is a potential of producing more than 10 t/ha. Drought is one of the major abiotic constraints contributing to this low productivity. Drought diminishes crop productivity mainly by causing premature leaf senescence. The ipt gene codes for...

Author(s): Leta Tulu Bedada, Miccah Songelael Seth, Steven Maina Runo, Wondyifraw Teffera, Charless Mugoya, Clet Wandui Masiga, Richard Okoth Oduor, Eduardo Blumewald and Francis Wachira

Transcriptional modulation of genes encoding nitrate reductase in maize (Zea mays) grown under aluminum toxicity

October 2016

The free aluminum (Al) content in soil can reach levels that are toxic to plants, and this has frequently limited increased productivity of cultures. Four genes encoding nitrate reductase (NR) were identified, named ZmNR1–4. With the aim of evaluating NR activity and the transcriptional modulation of the ZmNR1, ZmNR2, ZmNR3, and ZmNR4 genes in leaves, 30-day-old hybrid maize BRAS 3010 plants were irrigated with a...

Author(s): Talita Cantú, Crislaine Emidio Vieira, Rafaélla David Piffer, Giovanna Carneiro Luiz and Silvia Graciele Hülse de Souza

Alternative tube caps on in vitro growth of Orbignya oleifera Burret.: An Arecaceae native cerrado domain

October 2016

The babassu (Orbignya oleifera Burret) is a palm that contains oilseeds whose biomass can be used for biofuel production. Due to difficulties in germinating the species, in vitro cultivation techniques are sought as a viable alternative to expand studies on germination. The objective of this study was to evaluate different types of tube caps for in vitro growth of babassu seedlings. Thus, zygotic embryos were inoculated...

Author(s): Mariluza Silva LEITE, Paula Sperotto Alberto FARIA, Flávia Dionísio PEREIRA, Fabiano Guimarães SILVA, Aurélio Rúbio NETO and João das Graças SANTANA

On-line methanol sensor system development for recombinant human serum albumin production by Pichia pastoris

October 2016

An on-line methanol sensor system was developed using a methanol probe, methanol sensor unit and peristaltic pump. The system was commanded using data acquisition (DAQ) and LabVIEW software. Calibration of the methanol sensor system was done in a medium environment with yeast cells during cells adaptation to methanol metabolism after glycerol feeding was stopped. The correlation equations between voltage output signal...

Author(s): Panchiga Chongchittapiban, Jӧrgen Borg, Yaowapha Waiprib, Jindarat Pimsamarn and Anan Tongta

Cloning and expression analysis of alcohol dehydrogenase (Adh) hybrid promoter isolated from Zea mays

October 2016

Hybrid promoters are created by shuffling of DNA fragments while keeping intact regulatory regions crucial of promoter activity. Two fragments of alcohol dehydrogenase (Adh) promoter from Zea mays were selected to generate hybrid promoter. Sequence analysis of both alcohol dehydrogenase promoter fragments through bioinformatics tools identified several crucial cis regulatory elements and transcription factors binding...

Author(s): Ammara Masood, Nadia Iqbal, Hira Mubeen, Rubab Zahra Naqvi, Asia Khatoon and Aftab Bashir

Production and characterization of endoglucanase secreted by Streptomyces capoamus isolated from Caatinga

October 2016

Cellulases are hydrolases of great importance to industries, especially due to their ability to produce ethanol via hydrolysis of cellulolytic materials. Actinomycetes are the producers of these enzymes, particularly the genus Streptomyces sp. The present study is the first report on the production and characterization of cellulolytic complex secreted by Streptomyces capoamus, isolated from the rhizosphere soil of...

Author(s): Rafael Lopes e Oliveira, Camila Beatriz Atanásio Borba, Sergio Duvoisin Junior, Patricia Melchionna Albuquerque, Gláucia Manoella de Souza Lima, Norma Buarque de Gusmão, Edmar Vaz de Andrade and Leonor Alves de Oliveira da Silva,

SIM2PeD– Intelligent monitoring system for prevention of diabetic foot

October 2016

Diabetes is an endocrine chronic disease that causes high blood sugar level, produced by retardation in, or deficiency of, glucose metabolism in the body of the individual with the disease. Neuropathies and/or angiopathies are complications of diabetes that result in changes in the lower limbs which subsequently evolve for the diabetic foot. Diabetic foot represents one of the most devastating complications of diabetes...

Author(s): Rodrigo Medeiros, Cicília Raquel Maia Leite, Suélia de Siqueira Rodrigues Fleury Rosa, Marina Pinheiro,  Luísiane de Ávila Santana and Jane Dulius

Lipase production from a wild (LPF-5) and a mutant (HN1) strain of Aspergillus niger

October 2016

In this study, a wild (LPF-5) and a mutant (HN1) strain of A. niger were compared for lipase production. Several physical parameters (carbon source, nitrogen source, pH, temperature and incubation period) were optimized for maximization of lipase production. Lipase activity between wild type and mutant strain were compared. Among all carbon sources, mixture of glucose (1%, w/v) and olive oil (1%, v/v) exhibited maximum...

Author(s): Arun Kumar Sharma, Vinay Sharma, Jyoti Saxena and Arindam Kuila

Comparison of protease production from newly isolated bacterial strains T5, T3 and H3 after immobilization

October 2016

Protease has gained a very important position in many industries such as food, pharmaceutical, chemical and leather industries. In this research, protease was obtained from bacteria. The bacterial strain was obtained from soil which was collected from different areas of Lahore, Pakistan. Fermentation medium (by using sub-merged fermentation technique) was incubated for 48 h at 37°C temperature and agitation speed of...

Author(s): Mina Ilyas

DNA markers reveal genetic structure and localized diversity of Ethiopian sorghum landraces

October 2016

North Eastern Ethiopia is a major sorghum-growing region. A total of 415 sorghum landraces were sampled to represent the range of agro-ecologies (three altitude ranges) as well as spatial heterogeneity, that is, 4 zones: North Welo, South Welo, Oromiya and North Shewa with each zone containing 2 to 5 districts. The landraces were genotyped with simple sequence repeats (SSR) and inter simple sequence repeats (ISSR)...

Author(s): Haile Desmae, David R. Jordan and Ian D. Godwin,

Molecular screening of rice (Oryza sativa L.) germplasm for Xa4, xa5 and Xa21 bacterial leaf blight (BLB) resistant genes using linked marker approach

October 2016

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has now become one of major rice diseases. During recent years, its occurrence has increased in Pakistan, particularly in ‘Kallar tract’ which is well-known for quality rice cultivation. Development of new resistant varieties by utilizing the available resistant gene-pool is most reliable breeding technique for the plant breeders. The...

Author(s): Muhammad Sabar, Tahira Bibi, Hafiz Umar Farooq, Zulqarnain Haider, Imad Naseem, Abid Mahmood and Muhammad Akhter

Drying kinetics of Baru almond (Dipteryx alata Vog) used for the production of biodiesel

October 2016

This study aimed to evaluate the drying kinetics of Baru (Dipteryx alata Vog), fit different mathematical models to the experimental values as a function of the water content, determine the diffusion coefficient and energy of Baru almonds during the drying process. Baru almonds were dried at 50, 60 and 70°C temperatures in a tray dryer until the water content reached 13% b.u. A non-linear regression analysis was...

Author(s): Paulo Cleber Mendonça Teixeira and Abraham Damian Giraldo Zuniga

Renal and hepatic histopathology of intraperitoneally administered potassium permanganate on Clarias gariepinus juveniles

October 2016

The biochemistry and histopathology of intraperitoneally administered potassium permanganate was investigated in Clarias gariepinus. Acute toxicity of the KMnO4 was determined by intraperitoneally injecting the fish with 0.0, 1.5, 2.0, 4.0 and 6.0 mg/kg. The 96 h lethal concentration (LC50) value obtained from the intraperitoneal injection of the juveniles of C. gariepinus with KMnO­4 was 2.001 mg/kg KMnO4 at 95%...

Author(s): Odo Gregory Ejikeme, Agwu Ekenma Juliana, Eke Felicia and Ikele Chika Bright and Ezea Celestine Osita

Development of ethanol production from cooking oil glycerol waste by mutant Enterobacter aerogenes

October 2016

Bacterial strains, capable of producing ethanol by using cooking oil glycerol waste (derived from biodiesel production) as sole C-source were screened from soil samples. Based on 16S rRNA sequence, the selected strain was identified as Enterobacter aerogenes G2WG. The sequence was submitted to GenBank and the accession number KU315428 was obtained. The genetic nature of the wild type strain was altered for enhancing...

Author(s): Thalisa Yuwa-amornpitak and Kannika Chookietwatana

Production, characterisation and flocculation mechanism of bioflocculant TMT-1 from marine Bacillus pumilus JX860616

October 2016

Bioflocculant from marine Bacillus pumilus JX860616 was characterised and its flocculation mechanism determined. The bacterium was identified by 16S rRNA and the bioflocculant was obtained through solvent extraction after optimum medium composition and culture conditions were established. The physicochemical analysis of the bioflocculant were obtained by scanning electron microscopic (SEM) equipped with elemental...

Author(s): Maliehe, T. S., Simonis, J., Basson, A. K., Reve, M., Ngema, S. and Xaba, P. S.

In vitro regeneration of disease free enset [Ensete ventricosum (Welw) Cheesman] planting materials from bacterial wilt diseased plants using shoot tip culture

October 2016

Enset is an important food crop produced in Ethiopia with great role in food security. The demand of the crop is increasing throughout the country. However, the production as a whole is decreasing due to devastation by enset bacterial wilt. The studies were conducted to develop the procedure for obtaining multiple disease free plantlets from infected enset plants. Shoot tip explants from infected suckers of three clones...

Author(s): Genene Gezahegn and Firew Mekbib

Screening of spontaneous castor bean accesses for genetic improvement programs

October 2016

The objective of this work was to identify, through the use of multivariate analysis, the spontaneous accesses of castor bean that show promising characteristics for inclusion in genetic improvement programs of this culture in Brazil. The study was conducted at the Paulista State University, Jaboticabal Campus. The accesses of castor bean seeds obtained were identified as ACS-001-CRSP, ACS-001-JASP, ACS-001-MASP,...

Author(s): Rener Luciano de Souza Ferraz, Jane Lima Batista, Marcelo de Andrade Barbosa, Ivomberg Dourado Magalhães, Alberto Soares de Melo and Antonio Sergio Ferraudo

Assessment of the genetic diversity of Kenyan coconut germplasm using simple sequence repeat (SSR) markers

October 2016

Genetic diversity and relationship among 48 coconut individuals (Cocos nucifera L.) collections from the Coastal lowland of Kenya were analyzed using 15 simple sequence repeat (SSR) primer pairs. Diversity parameters were calculated using Popgene Software version 1.31. The gene diversity values ranged from 0.0408 (CAC68) to 0.4861 (CAC23) with a mean value of 0.2839. The polymorphic information content (PIC) values...

Author(s): Maurice E. Oyoo, Najya Muhammed, Kimani N. Cyrus and Simon M. Githiri

Evaluation of various pesticides-degrading pure bacterial cultures isolated from pesticide-contaminated soils in Ecuador

October 2016

Due to the intensive use of pesticides within the greenhouse-rose production, remediation of polluted soils has become a hot topic for researchers in recent decades. Several bacterial strains having the ability to utilize various pesticides as a sole source of carbon and energy were isolated from pesticide-contaminated soils of Urcuquí, Ecuador. According to phenotypical, physiological, and biochemical...

Author(s): Pérez Mónica, Rueda O. Darwin, Bangeppagari Manjunatha, Johana J. Zúñiga, Ríos Diego, Rueda B. Bryan, Sikandar I. Mulla and Naga R. Maddela

Use of response surface methodology to optimize the drying conditions of a bioactive ingredient derived from the African opaque sorghum beer

October 2016

The present work aims to optimize the heat drying conditions of a bioactive feed ingredient derived from the African opaque sorghum beer. The bioactive ingredient was dried at various temperatures 35 to 50°C and times 5 to 24 h. The effects of the drying conditions on the dry matter, water activity, pH, titratable acidity, bacteriocin, ethanol, lactic acid, yeasts, lactic acid bacteria contents and antimicrobial...

Author(s): I. B. Chabi, A. P. P. Kayodé, B. P. Agbobatinkpo, A. Adénilé, O. A. Mamadou, G. Vieira-Dalodé, L. Baba-Moussa and J. T. C. Codjia

Enzymatic regulation of organic acid metabolism in an alkali-tolerant halophyte Chloris virgata during response to salt and alkali stresses

October 2016

Chloris virgata, an alkali-tolerant halophyte, was chosen as the test material for our research. The seedlings of C. virgata were treated with varying salt and alkali stress. First, the composition and content of organic acids in shoots were analyzed and the results indicated that there was not only a significant increase in total organic acids, but there were also obvious changes in different components of organic...

Author(s): Huan Wang, Bing Bai, Ziyu Bai, Lijun Shi, Jinlin Ye, Siwen Fan, Huahua Xu and Decheng Shi

Comparison of four nonlinear growth models for effective exploration of growth characteristics of turbot Scophthalmus maximus fish strain

October 2016

This study was conducted to compare the effectiveness for non-linear growth models designated as Chapman-Richards, Gompertz, Logistic and von Bertalanffy for selection of fast-growing fish strain of turbot Scophthalmus maximus. These models were compared using the goodness of fit (the coefficient of determination (R2) and the mean square error (MSE)) and the Akaike information criterion (AIC) and the growth...

Author(s): Xin’an Wang,, and Aijun Ma,,

Genetic determinants of antifungal resistance in Candida species

October 2016

In the previous decades, it has been an increase in cases of resistance to antifungal agents used in the prophylaxis and treatment of infections caused by Candida species. The emergence of resistance to drug classes, it is usually explained by genome alterations ranging from point mutations to gain or loss of whole chromosomes. Therefore, identify and understand into the molecular mechanisms that underlie resistance of...

Author(s): Danielly Beraldo dos Santos Silva, Alexéia Barufatti Grisolia and Kelly Mari Pires de Oliveira

Phylogenetic relationships of the genus Quercus L. (Fageceae) from three different sections

October 2016

In this study, the genetic diversity of 6 oak species known as Quercus coccifera L., Q. robur L., Q. infectoria Oliver, Q. cerris L., Q. ithaburensis subsp. macrolepsis (Kotschy) Hedge and Yalt. and Q. trojana P.B. Webb  in 18 populations was screened using the randomly amplified polymorphic DNA method (RAPD).  10 RAPD primers giving the best results produced 262 total loci. The highest and lowest band sizes...

Author(s): Yılmaz Aykut

Antioxidant assessment on promethazinr HCl decomposition using RP-HPLC assay method

October 2016

The objective of this study was to investigate the effect of different sodium metabisulfite (SMBS) concentrations under a variety of ICH recommended test conditions. An attempt was made to test the feasibility of increasing shelf life when stored under different conditions. The promethazine hydrochloride (HCL) sample solutions used according to USP 24 and BP 1999 were prepared using different concentration of sodium...

Author(s): Rasha Saad, Heyam Saad Ali, Babiker M. A. Elhaj and Mai Al Ajaji

Molecular identification of Anopheles gambiae sensu stricto Giles (formerly Anopheles gambiae Savannah Form) in Kamuli District, Uganda

September 2016

Anopheles gambiae sensu stricto Giles (formerly A. gambiae S molecular form), the largely anthropophilic species, is reportedly the most important malaria vector in Uganda among the A. gambiae complex species. Indoor and outdoor human-biting mosquitoes were caught for four consecutive nights in each of 48 households in Kamuli district using human-baited bed net traps for subsequent identification of the principal...

Author(s): Fredrick George Kabbale, Anne Margaret Akol, John Baptist Kaddu, Enock Matovu, Anne Kazibwe, Anges Yadouleton and Ambrose Winston Onapa

In vitro methods for mutation induction in potato (Solanum tuberosum L.)

September 2016

Potato (Solanum tuberosum L.) is an important vegetable and staple crop worldwide and mainly propagated vegetatively. Breeding of potato is problematic and therefore induced mutation is an attractive means of improving the crop. In vitro culture systems, and especially the production of micro-tubers, are ideal for such purposes in potato improvement. Radio-sensitivity testing (growth reduction, GR and lethal dose, LD)...

Author(s): Souleymane Bado, Matumelo Alice Rafiri, Kaoutar El-Achouri, Enoch Sapey, Stephan Nielen, Abdelbagi Mukhtar Ali Ghanim, Brian Peter Forster, and Margit Laimer

Natural occurrence and pathogenicity of Xanthomonas bacteria on selected plants

September 2016

The bacterial genus Xanthomonas consists of several species of economic importance, among which Xanthomonas campestris pv.musacearum (Xcm), the cause of enset and banana wilt is the most important in tropical Africa. However, the natural occurrence and host range of this species is yet to be clarified. The objectives of this study were to verify the presence of Xanthomonas bacteria on plants growing in and around...

Author(s): Alemayehu Chala, Tadesse Kebede and Guy Blomme

Development of simple kinetic models and parameter estimation for simulation of recombinant human serum albumin production by Pichia pastoris

September 2016

In order to describe and predict the growth and expression of recombinant proteins by using a genetically modified Pichia pastoris, we developed a number of unstructured models based on growth kinetic equation, fed-batch mass balance and the assumptions of constant cell and protein yields. The growth of P. pastoris on both glycerol and methanol could be represented by Monod kinetic equation. A simple simulation...

Author(s): Panchiga Chongchittapiban, Jӧrgen Borg, Yaowapha Waiprib, Jindarat Pimsamarn and Anan Tongta

Improved embryogenic callus induction and plant regeneration in big bluestem (Andropogon gerardii Vitman), a potential bioenergy feedstock

September 2016

The objective of this study was to develop an efficient regeneration protocol in big bluestem, a potential feedstock that produces huge biomass. Embryogenic calli were induced from the seeds of cultivars, Kaw and Earl, on Murashige and Skoog (MS) medium with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.2, 0.5, 1.0, 2.0, 3.0 and 5.0 mg l-1) alone or in combination with 6-benzylaminopurine (BA)...

Author(s): Pramod Pantha, Sathish Kumar Ponniah, Sixte Ntamatungiro and Muthusamy Manoharan

A novel sampler for limnological investigation in developing world

September 2016

A simple pump sampler for shallow water bodies, constructed with materials that are affordable and readily available in the local market is presented. It is simple to operate and no electric motor is required. The challenge of cost of acquisition of conventional samplers for sampling the lentic habitat, especially small water bodies such as small natural lakes, rivers, streams and reservoirs for fisheries and water...

Author(s): Nkechinyere O. Nweze

Evaluation of the simultaneous effects of a heat stabilized starter concentration and the duration of fermentation on the quality of the opaque sorghum beer

September 2016

This study evaluates the simultaneous effect of dried starter concentration and fermentation duration on the quality characteristics of the African opaque sorghum beer using response surface methodology. The aim was to improve the beer quality and to optimize its fermentation process. Results show that the granule starter concentration and the fermentation duration have significant effects on the dry matter, refractive...

Author(s): Yves Kadjogbé Djegui, Adéchola Pierre Polycarpe Kayodé, Joseph Dossou and Joseph Djidjoho Hounhouigan

Preliminary investigation into the chemical composition of the invasive brown seaweed Sargassum along the West Coast of Ghana

September 2016

The arrival of the invasive brown seaweed Sargassum in the Western Region of Ghana was first reported in 2009. This impacted negatively on biodiversity, tourism and the livelihoods of the coastal communities. The objectives of the study among others were to identify and determine the nutritional and toxicological contents of the seaweed. Twenty four samples collected from 6 zones along the Western Region were analysed....

Author(s): Gloria Naa Dzama Addico and Kweku Amoako Atta deGraft-Johnson

Characterisation of Saccharomyces cerevisiae hybrids selected for low volatile acidity formation and the production of aromatic Sauvignon blanc wine

September 2016

Wine yeasts (Saccharomyces cerevisiae) vary in their ability to develop the full aroma potential of Sauvignon blanc wine due to an inability to release volatile thiols. Subsequently, the use of ‘thiol-releasing’ wine yeasts (TRWY) has increased in popularity. However, anecdotal evidence suggests that some commercially available TRWY intermittently exhibit undesirable characteristics for example, volatile...

Author(s): Hart R.S., Jolly N.P., Mohamed G., Booyse M. and Ndimba B.K.

Growth, ectomycorrhization and biochemical parameters of Quercus suber L. seedlings under drought conditions

September 2016

Drought conditions are the major constraint to the early establishment of Quercus suber species. However, drought responses of this species depend on provenances. The objective of this study was to obtain more comprehensive knowledge on the influence of drought conditions on the response of Q. suber L. seedlings originating from Algeria. Soil water status in soil (SWC) and relative water content (RWC) in leaves,...

Author(s): Henia Daoudi, Arezki Derridj, Lynda Hannachi and Jean Philippe Mevy

Coating with fungicide and different doses of fertilizer in vinhatico seeds

September 2016

Plathymenia reticulata Benth occurs in open formations of the Brazilian Cerrado and Atlantic Forest. The seeds are affected by pathogens that reduce germination. The use of fungicides is defined for many species, but, for forest species, further studies are necessary. Thus, the aim of this study was to evaluate and identify the physical and physiological quality of mahogany seeds that are coated with fertilizer and...

Author(s): Priscila Gonçalves Figueiredo de Sousa, Henrique Duarte Vieira, Mariá Moraes Amorim and Amanda Justino Acha

Somatic polyploidization and characterization of induced polyploids of Dioscorea rotundata and Dioscorea cayenensis

September 2016

Genetic improvement of major food yams is constrained by a number of factors, such as the scarcity of flowers and lack of synchronization between male and female flowering. Consequently, somaclonal variation including somatic polyploidization has been considered as a useful tool in yam breeding. Somatic polyploidization and its effect on phenotypic traits of Dioscorea species such as D. alata, D. japonica and D....

Author(s): Pachakkil Babil, Midori Iino, Yukiko Kashihara, Ryo Matsumoto, Hidehiko Kikuno, Antonio Lopez-Montes and Hironobu Shiwachi

Callus induction, direct and indirect organogenesis of ginger (Zingiber officinale Rosc)

September 2016

The present study aimed to induce callus, direct and indirect organogenesis of ginger (Zingiber officinale Rosc) by using Murashige and Skoog (MS) medium fortified with different concentrations and combinations of growth regulators. Shoot tip, in vitro leaf and root segments were used as explants to induce callus by MS medium containing (0.00 as control, 0.5, 1.00, 2.00 and 3.00 mg/L) of 2,4-dichloro-phenoxyacetic acid...

Author(s): Ammar Mohammed Ahmed Ali, Mawahib ElAmin Mohamed El-Nour and Sakina Mohamed Yagi

Antibacterial potential of silver nanoparticle synthesized by marine actinomycetes in reference with standard antibiotics against hospital acquired infectious pathogens

September 2016

Multi resistance to antibiotics is a serious and disseminated clinical problem, common to several new compounds that block the resistance mechanism. The present study aimed at the comparative study of silver nanoparticles synthesized through actinomycetes and their antimicrobial metabolites with standard antibiotic. Marine actinomycetes collected from Gulf mannar costal region, Kayalpatnam, located at Tuticorin...

Author(s): Mohamed Mahroop Raja M. and Ahmed John S.

Perennial soybean seeds coated with high doses of boron and zinc

September 2016

The objective of this work was to study combinations of high doses of boron (B) and zinc (Zn) in the recoating of perennial soybean seeds, in order to provide these nutrients to the future plants. The physical, physiological and nutritional characteristics of the coated seeds and initial development of plants in a greenhouse were evaluated. Tests carried out in the laboratory were conducted in a completely randomized...

Author(s): Amanda Justino Acha, Henrique Duarte Vieira and Marta Simone Mendonça Freitas

Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene

September 2016

Jatropha curcas L. oil has been shown to be suitable for the production of biodiesel. However, this species has not been domesticated yet. Genetic breeding through conventional methods is time consuming and costly, hence, genetic transformation could contribute positively to the improvement of interesting traits. Although in vitro regeneration and stable genetic transformation has been pursued for several years,...

Author(s): Mariana Crotti Franco, Kleber Alves Gomes, Marcel Mamede de Carvalho Filho, Ricardo Harakava, Nicolas Carels, Walter José Siqueira, Rodrigo Rocha Latado and Daniela de Argollo Marques

Germination, growth and physiological responses of Senegalia senegal (L.) Britton, Vachellia seyal (Delile) P. Hurter and Prosopis juliflora (Swartz) DC to salinity stress in greenhouse conditions

September 2016

Salinity is among the most widespread environmental threats to global plant production, especially in arid and semi-arid climates. Thus, the selection of salt tolerant species is necessary for sustainable plant productivity. The purpose of this study was to measure and understand the salt tolerance of three multipurpose trees used in reforestation programs in many Sahelian countries (Senegalia senegal, Syn. Acacia...

Author(s): Dioumacor Fall,, Niokhor Bakhoum, Fatoumata Fall,, Fatou Diouf, Mamadou O. Ly, Mayécor Diouf, Djamel Gully, Valérie Hocher and Diégane Diouf,

Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis × Elaeis oleifera)

September 2016

Oil palm hybrid BRS Manicoré is important for plantations in the north of Brazil, as it is resistant to fatal yellowing and is compact. Seed germination is slow and reduced, so somatic embryogenesis is a promising alternative for its propagation. Two kinds of starting explants were used: Zygotic embryos (ZE) and thin cell layers (TCL) from the base of seedling aerial parts. Two culture medium formulae were...

Author(s): Bonetti, K. A. P., Nesi, J., Quisen, R. C. and Quoirin, M.

Genetic diversity of rice (Oryza sativa) germplasm from six countries using simple sequence repeats markers

September 2016

This experiment was carried out to determine the genotypic variation among rice (Oryza sativa) accessions using simple sequence repeats (SSR) markers. In the present study, a total of 12 SSR markers were used across 87 rice accessions from six countries. NTSYS-pc and PowerMarker software were used for data analysis. Six primers out of these 12 primers showed DNA amplification and polymorphism among the 87 rice...

Author(s): Ibrahim Soe, Richard Akromah, Daniel Nyadanu, Maxwell Darko Asante and Alex Tamu

The rolling circle amplification and next generation sequencing approaches reveal genome wide diversity of Kenyan cassava mosaic geminivirus

September 2016

Rolling circle amplification is a simple approach of enriching populations of single-stranded DNA plant begomovirus genomes (genus, Begomovirus; family, Geminiviridae). This is an innovative approach that utilizes the robustness of the bacteriophage phi29 DNA polymerase used in circle amplification, together with deep sequencing using Illumina Miseq and bioinformatics to assess population diversity of begomoviruses in...

Author(s): T. M. Kathurima, , E. M. Ateka, A. B. Nyende and T. A. Holton

Influence of recirculation rate on the performance of a combined anaerobic-aerobic reactor applied to the removal of carbon and nitrogen from poultry slaughterhouse wastewater

September 2016

The objective of this study was to evaluate a combined anaerobic-aerobic upflow fixed-bed reactor with liquid phase recirculation for the removal of nitrogen and organic matter from poultry slaughterhouse wastewater. The reactor performance was evaluated with a hydraulic retention time (HRT) of 11 h and three different recirculation rates (R=0.5; 1 and 2). The highest nitrogen removal efficiency value was obtained with...

Author(s): Carla L. Lopes, Adriana N. de Lima Model, Jackeline T. Gotardo, Fernando H. Passig, Eloy L. de Mello, Benedito M. Gomes and Simone D. Gomes

Comparison between Giemsa and Van Geison stains in demonstration of collagen fibers (Kosti-2016)

September 2016

Trichrome stain (such as Van Geison) is usually used in histopathology laboratory for demonstration of collagenic fibers. Lack of selectivity and tendency of stain to fade makes van Gieson not ideal for collagen demonstration. This study was aimed to compare between Giemsa's and van Gieson's stains in collagen fibers demonstration. Twenty biopsies were obtained from rabbit’s skin after anesthesia by...

Author(s): Ziyad Mudasir, Salma Adil and Ahmed Ibn Edriss

Ex-vivo evaluation of crab shell chitosan as absorption enhancer in ciprofloxacin tablet formulation

September 2016

This study was aimed at evaluating crab shell chitosan as absorption enhancer in ciprofloxacin tablet formulation using the ex-vivo model. Six batches of ciprofloxacin tablets containing varying concentrations of crab shell-derived chitosan ranging from 0 to 5% w/w at 1% w/w intervals were produced. Batch CTS-0 containing no chitosan served as the control. The crushing strength, friability, disintegration time,...

Author(s): Emmanuel O. Olorunsola, Timma O. Uwah, Olubunmi J. Olayemi and Unyime B. Etukudo

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