African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

AJB Articles

September 2016

In vitro methods for mutation induction in potato (Solanum tuberosum L.)

Potato (Solanum tuberosum L.) is an important vegetable and staple crop worldwide and mainly propagated vegetatively. Breeding of potato is problematic and therefore induced mutation is an attractive means of improving the crop. In vitro culture systems, and especially the production of micro-tubers, are ideal for such purposes in potato improvement. Radio-sensitivity testing (growth reduction, GR and lethal dose, LD)...

Author(s): Souleymane Bado, Matumelo Alice Rafiri, Kaoutar El-Achouri, Enoch Sapey, Stephan Nielen, Abdelbagi Mukhtar Ali Ghanim, Brian Peter Forster, and Margit Laimer

September 2016

Natural occurrence and pathogenicity of Xanthomonas bacteria on selected plants

The bacterial genus Xanthomonas consists of several species of economic importance, among which Xanthomonas campestris pv.musacearum (Xcm), the cause of enset and banana wilt is the most important in tropical Africa. However, the natural occurrence and host range of this species is yet to be clarified. The objectives of this study were to verify the presence of Xanthomonas bacteria on plants growing in and around...

Author(s): Alemayehu Chala, Tadesse Kebede and Guy Blomme

September 2016

Development of simple kinetic models and parameter estimation for simulation of recombinant human serum albumin production by Pichia pastoris

In order to describe and predict the growth and expression of recombinant proteins by using a genetically modified Pichia pastoris, we developed a number of unstructured models based on growth kinetic equation, fed-batch mass balance and the assumptions of constant cell and protein yields. The growth of P. pastoris on both glycerol and methanol could be represented by Monod kinetic equation. A simple simulation...

Author(s): Panchiga Chongchittapiban, Jӧrgen Borg, Yaowapha Waiprib, Jindarat Pimsamarn and Anan Tongta

September 2016

Improved embryogenic callus induction and plant regeneration in big bluestem (Andropogon gerardii Vitman), a potential bioenergy feedstock

The objective of this study was to develop an efficient regeneration protocol in big bluestem, a potential feedstock that produces huge biomass. Embryogenic calli were induced from the seeds of cultivars, Kaw and Earl, on Murashige and Skoog (MS) medium with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.2, 0.5, 1.0, 2.0, 3.0 and 5.0 mg l-1) alone or in combination with 6-benzylaminopurine (BA)...

Author(s): Pramod Pantha, Sathish Kumar Ponniah, Sixte Ntamatungiro and Muthusamy Manoharan

September 2016

A novel sampler for limnological investigation in developing world

A simple pump sampler for shallow water bodies, constructed with materials that are affordable and readily available in the local market is presented. It is simple to operate and no electric motor is required. The challenge of cost of acquisition of conventional samplers for sampling the lentic habitat, especially small water bodies such as small natural lakes, rivers, streams and reservoirs for fisheries and water...

Author(s): Nkechinyere O. Nweze

September 2016

Evaluation of the simultaneous effects of a heat stabilized starter concentration and the duration of fermentation on the quality of the opaque sorghum beer

This study evaluates the simultaneous effect of dried starter concentration and fermentation duration on the quality characteristics of the African opaque sorghum beer using response surface methodology. The aim was to improve the beer quality and to optimize its fermentation process. Results show that the granule starter concentration and the fermentation duration have significant effects on the dry matter, refractive...

Author(s): Yves Kadjogbé Djegui, Adéchola Pierre Polycarpe Kayodé, Joseph Dossou and Joseph Djidjoho Hounhouigan

September 2016

Preliminary investigation into the chemical composition of the invasive brown seaweed Sargassum along the West Coast of Ghana

The arrival of the invasive brown seaweed Sargassum in the Western Region of Ghana was first reported in 2009. This impacted negatively on biodiversity, tourism and the livelihoods of the coastal communities. The objectives of the study among others were to identify and determine the nutritional and toxicological contents of the seaweed. Twenty four samples collected from 6 zones along the Western Region were analysed....

Author(s): Gloria Naa Dzama Addico and Kweku Amoako Atta deGraft-Johnson

September 2016

Characterisation of Saccharomyces cerevisiae hybrids selected for low volatile acidity formation and the production of aromatic Sauvignon blanc wine

Wine yeasts (Saccharomyces cerevisiae) vary in their ability to develop the full aroma potential of Sauvignon blanc wine due to an inability to release volatile thiols. Subsequently, the use of ‘thiol-releasing’ wine yeasts (TRWY) has increased in popularity. However, anecdotal evidence suggests that some commercially available TRWY intermittently exhibit undesirable characteristics for example, volatile...

Author(s): Hart R.S., Jolly N.P., Mohamed G., Booyse M. and Ndimba B.K.

September 2016

Growth, ectomycorrhization and biochemical parameters of Quercus suber L. seedlings under drought conditions

Drought conditions are the major constraint to the early establishment of Quercus suber species. However, drought responses of this species depend on provenances. The objective of this study was to obtain more comprehensive knowledge on the influence of drought conditions on the response of Q. suber L. seedlings originating from Algeria. Soil water status in soil (SWC) and relative water content (RWC) in leaves,...

Author(s): Henia Daoudi, Arezki Derridj, Lynda Hannachi and Jean Philippe Mevy

September 2016

Coating with fungicide and different doses of fertilizer in vinhatico seeds

Plathymenia reticulata Benth occurs in open formations of the Brazilian Cerrado and Atlantic Forest. The seeds are affected by pathogens that reduce germination. The use of fungicides is defined for many species, but, for forest species, further studies are necessary. Thus, the aim of this study was to evaluate and identify the physical and physiological quality of mahogany seeds that are coated with fertilizer and...

Author(s): Priscila Gonçalves Figueiredo de Sousa, Henrique Duarte Vieira, Mariá Moraes Amorim and Amanda Justino Acha

September 2016

Somatic polyploidization and characterization of induced polyploids of Dioscorea rotundata and Dioscorea cayenensis

Genetic improvement of major food yams is constrained by a number of factors, such as the scarcity of flowers and lack of synchronization between male and female flowering. Consequently, somaclonal variation including somatic polyploidization has been considered as a useful tool in yam breeding. Somatic polyploidization and its effect on phenotypic traits of Dioscorea species such as D. alata, D. japonica and D....

Author(s): Pachakkil Babil, Midori Iino, Yukiko Kashihara, Ryo Matsumoto, Hidehiko Kikuno, Antonio Lopez-Montes and Hironobu Shiwachi

September 2016

Callus induction, direct and indirect organogenesis of ginger (Zingiber officinale Rosc)

The present study aimed to induce callus, direct and indirect organogenesis of ginger (Zingiber officinale Rosc) by using Murashige and Skoog (MS) medium fortified with different concentrations and combinations of growth regulators. Shoot tip, in vitro leaf and root segments were used as explants to induce callus by MS medium containing (0.00 as control, 0.5, 1.00, 2.00 and 3.00 mg/L) of 2,4-dichloro-phenoxyacetic acid...

Author(s): Ammar Mohammed Ahmed Ali, Mawahib ElAmin Mohamed El-Nour and Sakina Mohamed Yagi

September 2016

Antibacterial potential of silver nanoparticle synthesized by marine actinomycetes in reference with standard antibiotics against hospital acquired infectious pathogens

Multi resistance to antibiotics is a serious and disseminated clinical problem, common to several new compounds that block the resistance mechanism. The present study aimed at the comparative study of silver nanoparticles synthesized through actinomycetes and their antimicrobial metabolites with standard antibiotic. Marine actinomycetes collected from Gulf mannar costal region, Kayalpatnam, located at Tuticorin...

Author(s): Mohamed Mahroop Raja M. and Ahmed John S.

September 2016

Perennial soybean seeds coated with high doses of boron and zinc

The objective of this work was to study combinations of high doses of boron (B) and zinc (Zn) in the recoating of perennial soybean seeds, in order to provide these nutrients to the future plants. The physical, physiological and nutritional characteristics of the coated seeds and initial development of plants in a greenhouse were evaluated. Tests carried out in the laboratory were conducted in a completely randomized...

Author(s): Amanda Justino Acha, Henrique Duarte Vieira and Marta Simone Mendonça Freitas

September 2016

Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene

Jatropha curcas L. oil has been shown to be suitable for the production of biodiesel. However, this species has not been domesticated yet. Genetic breeding through conventional methods is time consuming and costly, hence, genetic transformation could contribute positively to the improvement of interesting traits. Although in vitro regeneration and stable genetic transformation has been pursued for several years,...

Author(s): Mariana Crotti Franco, Kleber Alves Gomes, Marcel Mamede de Carvalho Filho, Ricardo Harakava, Nicolas Carels, Walter José Siqueira, Rodrigo Rocha Latado and Daniela de Argollo Marques

September 2016

Germination, growth and physiological responses of Senegalia senegal (L.) Britton, Vachellia seyal (Delile) P. Hurter and Prosopis juliflora (Swartz) DC to salinity stress in greenhouse conditions

Salinity is among the most widespread environmental threats to global plant production, especially in arid and semi-arid climates. Thus, the selection of salt tolerant species is necessary for sustainable plant productivity. The purpose of this study was to measure and understand the salt tolerance of three multipurpose trees used in reforestation programs in many Sahelian countries (Senegalia senegal, Syn. Acacia...

Author(s): Dioumacor Fall,, Niokhor Bakhoum, Fatoumata Fall,, Fatou Diouf, Mamadou O. Ly, Mayécor Diouf, Djamel Gully, Valérie Hocher and Diégane Diouf,

September 2016

Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis × Elaeis oleifera)

Oil palm hybrid BRS Manicoré is important for plantations in the north of Brazil, as it is resistant to fatal yellowing and is compact. Seed germination is slow and reduced, so somatic embryogenesis is a promising alternative for its propagation. Two kinds of starting explants were used: Zygotic embryos (ZE) and thin cell layers (TCL) from the base of seedling aerial parts. Two culture medium formulae were...

Author(s): Bonetti, K. A. P., Nesi, J., Quisen, R. C. and Quoirin, M.

September 2016

Genetic diversity of rice (Oryza sativa) germplasm from six countries using simple sequence repeats markers

This experiment was carried out to determine the genotypic variation among rice (Oryza sativa) accessions using simple sequence repeats (SSR) markers. In the present study, a total of 12 SSR markers were used across 87 rice accessions from six countries. NTSYS-pc and PowerMarker software were used for data analysis. Six primers out of these 12 primers showed DNA amplification and polymorphism among the 87 rice...

Author(s): Ibrahim Soe, Richard Akromah, Daniel Nyadanu, Maxwell Darko Asante and Alex Tamu

September 2016

The rolling circle amplification and next generation sequencing approaches reveal genome wide diversity of Kenyan cassava mosaic geminivirus

Rolling circle amplification is a simple approach of enriching populations of single-stranded DNA plant begomovirus genomes (genus, Begomovirus; family, Geminiviridae). This is an innovative approach that utilizes the robustness of the bacteriophage phi29 DNA polymerase used in circle amplification, together with deep sequencing using Illumina Miseq and bioinformatics to assess population diversity of begomoviruses in...

Author(s): T. M. Kathurima, , E. M. Ateka, A. B. Nyende and T. A. Holton

September 2016

Influence of recirculation rate on the performance of a combined anaerobic-aerobic reactor applied to the removal of carbon and nitrogen from poultry slaughterhouse wastewater

The objective of this study was to evaluate a combined anaerobic-aerobic upflow fixed-bed reactor with liquid phase recirculation for the removal of nitrogen and organic matter from poultry slaughterhouse wastewater. The reactor performance was evaluated with a hydraulic retention time (HRT) of 11 h and three different recirculation rates (R=0.5; 1 and 2). The highest nitrogen removal efficiency value was obtained with...

Author(s): Carla L. Lopes, Adriana N. de Lima Model, Jackeline T. Gotardo, Fernando H. Passig, Eloy L. de Mello, Benedito M. Gomes and Simone D. Gomes

September 2016

Comparison between Giemsa and Van Geison stains in demonstration of collagen fibers (Kosti-2016)

Trichrome stain (such as Van Geison) is usually used in histopathology laboratory for demonstration of collagenic fibers. Lack of selectivity and tendency of stain to fade makes van Gieson not ideal for collagen demonstration. This study was aimed to compare between Giemsa's and van Gieson's stains in collagen fibers demonstration. Twenty biopsies were obtained from rabbit’s skin after anesthesia by...

Author(s): Ziyad Mudasir, Salma Adil and Ahmed Ibn Edriss

September 2016

Ex-vivo evaluation of crab shell chitosan as absorption enhancer in ciprofloxacin tablet formulation

This study was aimed at evaluating crab shell chitosan as absorption enhancer in ciprofloxacin tablet formulation using the ex-vivo model. Six batches of ciprofloxacin tablets containing varying concentrations of crab shell-derived chitosan ranging from 0 to 5% w/w at 1% w/w intervals were produced. Batch CTS-0 containing no chitosan served as the control. The crushing strength, friability, disintegration time,...

Author(s): Emmanuel O. Olorunsola, Timma O. Uwah, Olubunmi J. Olayemi and Unyime B. Etukudo

September 2016

Optimization of somatic embryogenesis procedure for commercial clones of Theobroma cacao L.

The first objective of this study was to assess and optimize somatic embryo production in a genetically diverse range of cacao genotypes. The primary and secondary somatic embryogenesis response of eight promising cacao clones and a positive control was evaluated using modified versions of standard protocols. The second objective was to optimize the efficiency of primary somatic embryogenesis for a commercially...

Author(s): Garcia Claudia, Corrêa Fabio, Findley Seth, Almeida Alex-Alan, Costa Marcio, Motamayor Juan Carlos, Schnell Ray and Marelli Jean-Philippe

September 2016

Physiological responses by Billbergia zebrina (Bromeliaceae) when grown under controlled microenvironmental conditions

Sucrose, the most commonly used carbon source in conventional in vitro culture, and limited air exchange in the culture containers are factors that affect the growth of in vitro-cultured plants. They may induce physiological disorders and decrease the survival rate of plants after transfer to ex vitro conditions. The aim of the present study was to analyze the effects of gas exchange and sucrose concentration on...

Author(s): João Paulo Rodrigues Martins, Veerle Verdoodt, Moacir Pasqual and Maurice De Proft

September 2016

Fruit production and quality of guava ‘Paluma’ as a function of humic substances and soil mulching

The uses of humic substances and organic residues for soil mulching have been more common during last years. This way, an experiment was carried out to evaluate the fruit quality and yield of guava cv. Paluma as a function of humic substances and organic soil mulching in Brazil. The experiment was performed from January 2012 to June 2013 (first trial) and from July to November 2013 (second trial) using a randomized...

Author(s): Leonardo Fonseca da Rocha, Lourival Ferreira Cavalcante, Járisson Cavalcante Nunes, Antônio Gustavo de Luna Souto, Alian Cássio Pereira Cavalcante, Ítalo Herbert Lucena Cavalcante and Walter Esfrain Pereira

September 2016

Growth and physiological aspects of bell pepper (Capsicum annuum) under saline stress and exogenous application of proline

This study aimed to evaluate growth and physiological aspects of ‘All Big’ bell pepper, under saline stress and exogenous application of proline on the leaves. The research was conducted in pots adapted as drainage lysimeters under greenhouse conditions, using sandy-loam eutrophic Regolithic Neosol, in the municipality of Campina Grande-PB, Brazil. The experiment was set in randomized blocks, in order to...

Author(s): Francisco Vanies da Silva Sá, Geovani Soares de Lima, João Batista dos Santos, Hans Raj Gheyi, Lauriane Almeida dos Anjos Soares, Lourival Ferreira Cavalcante, Emanoela Pereira de Paiva and Leandro de Pádua Souza

September 2016

Protective effects of some fruit juices with low-fat diet on rat testis damaged by carbon tetrachloride: A genetic and histological study

Oxidative stress, free radical, lipid peroxidation and antioxidant have become a common expression with most disease and methods for protection. Carbon tetrachloride (CCl4) is an industrial solvent which has destructive effects on a cell while most fruit juices have antioxidant effects. The aim of this study was to investigate the protective role of fruit juice on testis after toxic effect with CCl4 through oxidative...

Author(s): Medhat R. Rehan, Amina M. G. Zedan, Samah A. El-Hashash, Mona A. Farid and Gehan A. El-Shafie

September 2016

Confirmation of antibodies against L-tryptophan-like epitope in human African trypanosomosis serological diagnostic

Antibodies directed against L-tryptophan epitope (WE - W for tryptophan, E for epitope), a constant epitope borne by variant surface glycoproteins (VSG), have been detected in sera of all 152 Human African Trypanosomosis (HAT) patients from Angola. The WE is present in VSG hydrophobic regions of the C terminal domains. In the assay, L-tryptophan was linked to bovine serum albumin (BSA) with glutaraldehyde to synthesize...

Author(s): Silla Semballa,, Romaric Nzoumbou-Boko, , Philippe Holzmuller , Paul Chuchana , Jean-loup Lemesre, Philippe Vincendeau, Pierrette Courtois, Sylvie Daulouède and Philippe Truc,

September 2016

Evaluation of phenolic compounds of two Lygeum spartum L. cytotypes

Lygeum spartum represents a natural barrier in the Algerian high plateaus against the advance of sand and the desertification. The plant is of interest due to its tolerance to environmental stress. There are two levels of ploïdy in this species; the diploid cytotype 2n = 16 which is confined in the locality of Ain Benkhllil and the polyploid cytotype 2n = 40 which thrives in semi-arid climates and has a large...

Author(s): Louerrad Yasmina and Kaid-Harche Meriem

September 2016

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Micropropagation requires controlling contamination that might compromise the success of the process. Thermal sterilization is traditionally used; however, costs deriving from equipment acquisition and maintenance render this technique costly. With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for...

Author(s): Ana Karolina Pais, Ana Paula da Silva, Joselita Cardoso de Souza, Silvio Lopes Teixeira, Juliana Martins Ribeiro, Ana Rosa Peixoto and Cristiane Domingos da Paz

August 2016

Pharmacological properties of cashew (Anacardium occidentale)

Anacardium occidentale L. is a tree native to Brazil, which is rich in phenolic lipids. Nowadays, the cashew bark (Cashew Nut Shell Liquid) has received great attention in the pharmaceutical industry, due to its economy, abundance and important chemical compounds. Net of cashew nut shell is classified according to the method of production of: (1) net of the shell of natural cashew nut (60-65% anacardic acid; 15-20%...

Author(s): Aracelli de Sousa Leite,, Md. Torequl Islam,, Antonio Luiz Gomes Júnior,,  João Marcelo de Castro e Sousa, Marcus Vinícius Oliveira Barros de Alencar,, Márcia Fernanda Correia Jardim Paz, Hercília Maria Lins Rolim, Maria das Graças Freire de Medeiros, Ana Amélia de Carvalho Melo-Cavalcante, and José Arimatéia Dantas Lopes,

August 2016

Aflatoxins: A silent threat in developing countries

Several mycotoxins are known to contaminate crop produce and processed forms but aflatoxins are the most common. They are mainly produced by fungi belonging to the genera Aspergillus and Penicillium. Cereals and their products which constitute the staples in most developing countries are particularly vulnerable to attack by aflatoxigenic fungi. Despite the potential health risk posed to animals and humans, many people...

Author(s): Elias Nortaa Kunedeb Sowley

August 2016

Phylogenetic analysis of 23S rRNA gene sequences of some Rhizobium leguminosarum isolates and their tolerance to drought

The phylogenetic relationships among thirteen Rhizobium leguminosarum  bv. viciae isolates collected from various geographical regions were studied by analysis of the 23S rRNA sequences. The average of genetic distance among the studied isolates was very narrow (ranged from 0.00 to 0.04) and the studied isolates formed two main groups based on cluster analysis. The isolates were tested for their growth on YMA...

Author(s): Khalid S. Abdel-Lateif, Omar A. Hewedy and Abdel Fattah M. El-Zanaty.

August 2016

Resistance of corn genotypes to fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

The objective of this study was to evaluate resistance mechanisms in 12 corn genotypes (transgenic hybrids: 30A91 PW, 20A78 HX, Impacto VIP 3, 20A55 HX, NS90 PRO 2, Maximus VIP 3, BX 1293YG, RB 9004 PRO, Feroz VIP 3, LG 6036 PRO; conventional: AG 1051 and variety: AL Bandeitante). Attractiveness, aversion to feeding, and antibiosis were evaluated via free and no choice tests in the laboratory. Attractiveness was...

Author(s): Lígia Alves de Paiva, Fernanda Corrêa, Cinthia Luzia Teixeira Silva, Tony de Lima Moura, Franciele Cristina da Silva, Marcio da Silva Araújo and Flávio Gonçalves de Jesus

August 2016

Genetic diversity assessment of farmers’ and improved potato (Solanum tuberosum) cultivars from Eritrea using simple sequence repeat (SSR) markers

Sixty three potato clones (51 farmers’ and 12 varieties) from Eritrea, 18 and 12 varieties from Kenya and Rwanda, respectively were characterized using 12 highly polymorphic simple sequence repeat (SSR) markers. The study was designed to assess the genetic diversity and varietal distinctness among the different samples. In total, 91 alleles ranging between 2 (STM1053) to 13 (STM0031) alleles per marker were...

Author(s): Biniam Mesfin Ghebreslassie, S. Mwangi Githiri, Tadesse Mehari, Remmy W. Kasili, Marc Ghislain  and Eric Magembe

August 2016

Efficiency and response of conilon coffee genotypes to nitrogen supply

The objective of the study was to differentiate genotypes with higher efficiency and responsiveness to nitrogen supply, to understand how the nitrogen supply can impact the dry matter allocation and the accumulation of this nutrient in the different plant compartments of genotypes of conilon coffee, cultivated under contrasting conditions of nitrogen availability in the soil. The plants were cultivated during 150 days...

Author(s): Lindomar Souza Machado, Lima Deleon Martins, Wagner Nunes Rodrigues, Daniel S. Ferreira, Adan Dezan Côgo, Marcelo Antônio Tomaz and José Francisco Teixeira do Amaral

August 2016

Phosphate solubilization and multiple plant growth promoting properties of rhizobacteria isolated from chickpea (Cicer aeritinum L.) producing areas of Ethiopia

Chickpea is one of the major legume crops widely grown in Ethiopia. The low availability of phosphorus in soil is among the stresses that constrain the production of this crop in the country. However, there are rhizobacteria capable of solubilizing insoluble forms of phosphorus in soil and make it available to the plant. Thus, this study was aimed at isolation and characterization of phosphate solubilizing bacteria from...

Author(s): Mulissa J. Midekssa, Carolin R. Löscher, Ruth A. Schmitz and Fassil Assefa

August 2016

Effect of packaging and chemical treatment on storage life and physicochemical attributes of tomato (Lycopersicon esculentum Mill cv. Roma)

Fresh fruits and vegetables are inherently more liable to deterioration under tropical conditions characterized by high ambient temperatures and humidity. In determining the effects of chemical treatment on tomatoes (Lycopersicon esculentum Mill cv. Roma), fruits purchased at turning stage of ripening were packaged in low density polyethylene bags (60 µ) containing wooden dust particles moistened with 400 ppm...

Author(s): Tonna A. Anyasi, Charles O. Aworh and Afam I. O. Jideani

August 2016

Response of chickpea (Cicer arietinum L.) to inoculation with native and exotic Mesorhizobium strains in Southern Ethiopia

A series of pot and two consecutive crop-year field experiments were conducted from 2011 to 2012 in Southern Ethiopia to determine the effectiveness of Mesorhizobium strains on two cultivars of chickpea (Shasho and Nattoli). The eight treatments included: Six rhizobial inoculants, the four best indigenous strains (Cp8, Cp41, Cp97 and Cp105); CpNSTC (National Soils Testing Center inoculant); and CpSK (Canadian...

Author(s): Wondwosen Tena, Endalkachew Wolde-Meskel and Fran Walley

August 2016

Evaluation methods used for phosphate-solubilizing bacteria

Phosphorus solubilizers naturally acidify rhizospheric soil and increase phosphorus availability; therefore, their evaluation may help to reduce phosphorus fertilizer use. This work aimed to evaluate the different selection methods and select inorganic phosphorus-solubilizing bacteria as potential plant-growth promoters. Bacterial isolates obtained from sugarcane roots and soil were tested using solid growth media...

Author(s): Clayton Albuquerque Sousa, Mario de Andrade Lira Junior, Giselle Gomes Monteiro Fracetto, Fernando José Freire, and Júlia Kuklinsky Sobral

August 2016

Efficient micropropagation of Citrus sinensis (L.) Osbeck from cotyledonary explants suitable for the development of commercial variety

In vitro regeneration of sweet orange (Citrus sinensis (L.) Osbeck Family: Rutaceae) has been performed via direct and indirect organogenesis. For indirect organogenesis, callus was induced and proliferated from leaf explants derived from in vitro grown seedlings on Murashige and Skoog (MS) media containing 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with benzyl adenine (BA) and α-naphthalene...

Author(s): Aseesh Pandey and Sushma Tamta

August 2016

Production of extracellular laccase from the newly isolated Bacillus sp. PK4

Laccase belongs to the family of multicopper oxidase and have wide range of biotechnological applications starting from the food industry to bioremediation. However, the use of laccase at commercial scale is hindered by factors like high enzyme cost, low activity and /or stability under given conditions. This study was carried out with aim of screening for extracellular thermostable laccase producing bacteria....

Author(s): Murugesan Rajeswari and Vembu Bhuvaneswari

August 2016

Polyhydroxyalkanoate (PHA) production by Lynisibacillus sp. strain UEA-20.171

PHA is a biodegradable and biocompatible natural thermoplastic produced from renewable bioresources and is hence attracting attention as a plastic material for use in the environment and medical fields. In the present study, the Lynisibacillus sp. strain UEA-20.171 was selected for production of polyhydroxyalkanoate (PHA) in bioreactor. The accumulation of polymer in the bacterium occurs when there is an imbalance in...

Author(s): Edilane Martins Ferreira Aldo Rodrigues de Lima Procópio Raimundo Carlos Pereira Junior Sandra Patricia Zanotto Rudi Emerson de Lima Procópio

August 2016

Assessment of biofuel potential of dead neem leaves (Azadirachta indica) biomass in Maroua town, Cameroon

Dead leaves of neem trees in the Sahelian urban zone are among the wastes that are underutilized, since it is either buried or burnt, and thus, contribute to increased environmental pollution. Unfortunately, the lack of information on the biomass and energy potentials of these wastes empedes any initiative for its industrial biomethanization. This study was investigated with the aim of evaluating the biofuel potentials...

Author(s): Tizé Koda Joël, Sinbai Marcel, Darman Roger Djouldé and Albert Ngakou

August 2016

Genetic determinism of oil acidity among some DELI oil palm (Elaeis guineensis Jacq.) progenies

The oil palm (Elaeis guineensis Jacq.) is cultivated mainly for crude palm oil (CPO) which is extracted from the mesocarp of fruits. The quality of CPO is generally impaired due to high acidity, as a result of the activity of a lipase present in the mesocarp of the fruits at maturity. The objective of this study was to establish the genetic determinism of “palm oil acidity” (POA) from E. guineensis. Acidity...

Author(s): Benoit Constant LIKENG-LI-NGUE, Joseph Martin BELL, Georges Franck NGANDO-EBONGUE, Godswill Ntsefong NTSOMBOH and Hermine Bille NGALLE

August 2016

Functional properties of milk drinks flavored with mangaba pulp and enriched with passion fruit bark flour

Dairy foods including dairy based drinks play an important role in human nutrition. The flour of the passion fruit peel has high potential for use in the enrichment dairy drinks improving the nutritional and technological qualities besides an alternative to reduce waste by-products in the food industry. Thus, this study aimed to evaluate the physical and chemical parameters, texture, color, chemical composition,...

Author(s): Lígia Campos de Moura, Marco Antônio Pereira da Silva, Geovana Rocha Plácido, Márcio Caliari, Diene Gonçalves Souza, Jéssica Leal Freitas e Souza, Juliana Aparecida Célia, Kênia Borges de Oliveira, Karen Martins Leão and Luiz Eduardo Costa do Nascimento

August 2016

Organic and conventional vegetables: Comparison of the physical and chemical characteristics and antioxidant activity

The objective of this research was to compare the physical and chemical characteristics and antioxidant activity of organic and conventional carrot (Daucus carota), green pepper (Capsicum annuum) and lettuce (Lactuca sativa). Five representative samples of  conventional vegetables, certified organic and non-certified organic vegetables were gotten from farms and supermarkets in the city of Rio de Janeiro. The...

Author(s): Fernanda de Oliveira Pereira, Renata dos Santos Pereira, Lana de Souza Rosa and Anderson Junger Teodoro

August 2016

Oxidative enzymes in coconut cultivars in response to Raoiella indica feeding

The increase in oxidative enzyme activities is related to diminished mite infestation. Some biological aspects of Raoiella indica Hirst reared on the coconut cultivars (‘Jamaican Tall’ (JT), ‘Malayan Yellow Dwarf’ (MYD), Niu Leka (NL) and a hybrid JT x MYD) were studied under laboratory conditions. Aditionally, changes in oxidative enzyme activities (peroxidase and polyphenol oxidase) as response...

Author(s): Carlos Vásquez, Marta Dávila, Naileth Méndez, María A. Jiménez and María F. Sandoval and Francisco J. Alcalá,

August 2016

Ovarian follicular dynamics in purebred and crossbred Boran cows in Ethiopia

Boran is an endangered breed of cattle indigenous to Ethiopia and the relatively poor understanding of its reproductive physiology has impeded efforts to maximize reproductive performance of the breed. This study characterized ovarian follicular dynamics in 9 purebred Boran and 8 Boran×Holstein (B×H) crossbred cows. Ovaries of all 17 cows were examined once per day for 61 consecutive days (encompassing three...

Author(s): Tamrat Degefa, Alemayehu Lemma, Jeilu Jemal, Gbremeskel Mamo, Azage Tegegne and Curtis R. Youngs

August 2016

Micropropagation of caçari under different nutritive culture media, antioxidants, and levels of agar and pH

The caçari (Myrciaria dubia) is a native fruit tree from Amazon with high concentrations of vitamin C. This study aimed to adjust a culture medium that meets the nutritional needs for the in vitro development of caçari, evaluating the effect of different concentrations and nutritive culture media, antioxidant, and levels of agar and pH. Three experiments were carried out in a completely randomized design:...

Author(s): Maria Da Conceicao Da Rocha Araujo, Edvan Alves Chagas, Maria Isabel Ribeiro Garcia, Sara Thiele Sobral Pinto, Pollyana Cardoso Chagas, Wagner Vendrame, Adamor Barbosa Mota Filho and Olisson Mesquita de Souza

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