African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

AJB Articles

Effect of organic matter, irrigation and soil mulching on the nutritional status and productivity of okra (Abelmoschus esculentus L.) in the semiarid region of Brazil

November 2016

Okra (Abelmoschus esculentus L) is an important vegetable crop currently cultivated in many parts of the world. The adoption of management strategies to promote the nutritional status and crop productivity in semiarid regions of Brazil is still poorly studied. Thus, this experiment was conducted to evaluate the effect of raising the level of organic matter in the soil, irrigation and soil mulching in the nutritional...

Author(s): Marcelo de Andrade Barbosa, Núbia Marisa Ferreira, Antônio Michael Pereira Bertino, Evandro Franklin de Mesquita, Lucia Helena Garófalo Chaves, Lourival Ferreira Cavalcante and Everlon Cid Rigobelo

Growth promotion mediated by endophytic fungi in cloned seedlings of Eucalyptus grandis x Eucalyptus urophylla hybrids

November 2016

Eucalyptus cultivation has expanded considerably in Brazilian systems, leading to the current search for technologies to optimize growing conditions and the production of seedlings in nurseries. Based on the understanding that the development of tree species such as Eucalyptus sp. can be influenced by endophytic fungi that act directly as plant growth-promoting species, cloned seedlings of Eucalyptus grandis x...

Author(s): Luciana Cristina Vitorino, Layara Alexandre Bessa, Ligia Guimarães Carvalho and Fabiano Guimarães Silva

Primary study on the components and main physico-chemical as well as biological properties of the oil of Alpinia galanga (L.) Wild in Phu Tho-Vietnam

November 2016

The oil of Alpinia galanga (L.) Willd in Phu Tho was obtained by steam distillation and dried with Na2SO4. By Gas chromatography-mass spectrometry (GC-MS) method, 29 components in the oil were predicted by comparing their retention times and molecular weights with the standards’. In particular, there were 10 hydrocarbons such as monoterpenes: 29.15%, sesquiterpenes: 21.06%, and 19 oxygenated components like...

Author(s): Nguyen Van Loi and Nguyen Quynh Uyen

A review of cashew (Anacardiumoccidentale L.) apple: Effects of processing techniques, properties and quality of juice

November 2016

A review including the processing techniques, properties and uses of cashew apple juice is reported. Cashew apple has multi-purpose; it can be processed to obtain human food. The process of cashew apple into several by-products can affect its nutritional, microbiological, and sensorial quality attributes. Therefore, clarification methods, thermal treatment and high hydrostatic pressure modify nutritional,...

Author(s): Emmanuelle Dedehou, Joseph Dossou, Victor Anihouvi and Mohamed M. Soumanou

Effect of whey storage on physicochemical properties, microstructure and texture profile of ricotta cheese

November 2016

The aim of this study is to evaluate the effect of whey storage period (0, 24, 48 and 72 h) on the physicochemical parameters, color, texture and microstructure of fresh ricotta during storage. Sweet whey and acid whey were evaluated based on titratable acidity, pH, fat, cryoscopy, and density, while ricotta was based on yield, fat, protein, ash, acidity, pH, moisture, total solids, color, texture, and microstructure....

Author(s): Jéssica Leal Freitas Souza, Marco Antônio Pereira da Silva, Rânio Cesar Francisco da Silva, Ruthele Moraes do Carmo, Rodolfo Gomes de Souza, Juliana Aparecida Célia, Kênia Borges de Oliveira, Geovana Rocha Plácido, Moacir Evandro Lage and Edmar Soares Nicolau

Influence of vermicompost humic acid on chlorophyll content and acclimatization in banana clone, Enano Guantanamero

November 2016

Vermicompost humic acids (VHA) promote plants’ growth because they have similar effects with auxins. The aim of this research was to evaluate the effect of VHA in some physiological indicators in the micropropagation and acclimatization phase of banana clone Enano Guantanamero. Six concentrations were used (0, 10, 20, 30, 40 and 50 mg L-1) to evaluate in vitro the number of leaves, total chlorophyll content and...

Author(s): Marcia Beatriz Moya Fernández, Esteban Sánchez Chávez, Daniel Cabezas Montero, Andrés Calderín García, Dany Marrero López, Eduardo F. Héctor Ardisana and Sandra Pérez Álvarez

Study of genetic determinism of harvest index in durum wheat (Triticum durum Desf) under semi-arid conditions

November 2016

Out of six varieties of durum wheat (Triticum durum Desf.), two local varieties with a low harvest index and four others with high harvest indices and short straw imported from France were studied in a diallel cross. The experiment was done in a complete randomized block design with three replications. It was done at the Research Station of the Institute of Technical Big Cultures of Khemis Miliana, located in the...

Author(s): Bousalhih, B., Mekliche, L., Aissat, A. and Sadek Benabbes Halim

Comparative phylogenetic analysis of intergenic spacers and small subunit rRNA gene sequences of two microsporidian isolates from Antheraea myllita

November 2016

Two microsporidian isolates extracted from infected tasar silkworms (Antheraea mylitta)  collected from forest area in Deoghar district, Jharkhand, India were subjected to PCR amplification using intergenic spacer (IGS) region and small subunit rRNA (SSU-rRNA) gene specific primers followed by cloning and sequencing. The IGS and SSU-rRNA gene sequences were analysed to derive the identity of the microspoidian...

Author(s): Wazid Hassan, B. Surendra Nath and Geetha N. Murthy

Isolation and characterization of methanogenic bacteria from brewery wastewater in Kenya

November 2016

The production of biogas from renewable resources is becoming a prominent feature of most developed and developing countries of the world. A study was undertaken to characterize methanogenic microbial community found in brewery waste water. Their performance with regards to methane production was also studied. Thirty-two isolates were obtained using brewer thyglycollate agar medium. Characterization of the isolates was...

Author(s): Sylvia Injete Murunga, Duncan Onyango Mbuge, Ayub Njoroge Gitau, Urbanus Ndungwa. Mutwiwa and Ingrid Namae Wekesa

Production of alkaline proteases by alkalophilic Bacillus subtilis during recycling animal and plant wastes

November 2016

The production of extracellular alkaline protease by Bacillus subtilis was studied with submerged fermentation. A new strain of Bacillus sp. was isolated from alkaline soil, which was able to produce extracellular alkaline protease. The production of alkaline protease involved the use of agricultural or animal wastes at pH 8 and temperatures at 37°C. Results showed that growing B. subtilis sub sp. subtilis under...

Author(s): Amira Hassan Al-Abdalall and Eida Marshid Al-Khaldi

Investigating the potential of Aucoumea klaineana Pierre sapwood and heartwood wastes to produce cellulosic ethanol

November 2016

This work investigates for the first time the different reactivity exhibited by sapwood and heartwood wastes under two conditions: They were pretreated with catalyzed ethanol organosolv in the presence of sulfuric acid and their pulps underwent enzymatic hydrolysis by Trichoderma reesei which causes them to release neutral sugars for the production of cellulosic ethanol.  Aucoumea klaineana Pierre (Okoumé)...

Author(s): Rodrigue Safou-Tchiama, Sébastien Ngwa Obame, Nicolas Brosse, Patrice Soulounganga and Timoléon Andzi Barhé

Comparison of seed priming techniques with regards to germination and growth of watermelon seedlings in laboratory condition

November 2016

Seeds of watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai, cv. Crimson Sweet] were used to investigate the effects of different priming techniques on seed germination and early seedling growth. The seeds were soaked in solutions of 0.2% gibberellin (50 mg L–1 gibberellic acid), 0.2% cytokinin (90 mg L–1 kinetin), 0.2% potassium nitrate (2 g L–1 KNO3), 0.2% calcium nitrate [2 g L–1...

Author(s): Willams Ferreira Souza Barbosa, Fábio Steiner, Leandro Castro Matias de Oliveira and Paulo Henrique and Menezes das Chagas

Linkage disequilibrium and association mapping of drought tolerance in cotton (Gossypium hirsutum L.) germplasm population from diverse regions of Pakistan

November 2016

Drought stress is a major abiotic stress that limits crop production. Molecular association mapping techniques through linkage disequilibrium (LD) can be effectively used to tag genomic regions involved in drought stress tolerance. With the association mapping approach, 90 genotypes of cotton Gossypium hirsutum, from diverse regions of Pakistan were used. The morpho-physiological traits of all genotypes were evaluated...

Author(s): Abdelhafiz Adam Dahab, Muhammad Saeed, Nada Babiker Hamza, Bahaeldeen Babiker Mohamed and Tayyab Husnain

Inbreeding depression in crosses of coerulea clones of Walker’s Cattleya (Cattleya walkeriana Gardner)

November 2016

Orchids are among the most beautiful flowers and endangered due to habitat destruction and over-collection. Cattleya walkeriana is one of the most beautiful flowers joining the small sized plant with medium large and heavily scented flowers. It is widely known and appreciated by its beautiful clones and it has much to offer to breeders because their plants have besides other attributes as small habit and big flowers,...

Author(s): Miléia Ricci Picolo, Ceci Castilho Custódio, Nelson Barbosa Machado-Neto

The protective role of honey against cytotoxicity of cadmium chloride in mice

November 2016

The present study aimed to investigate the honey (HY) protective role in opposition to cadmium (Cd) induced chromosomal aberrations of bone marrow and sperm abnormalities. Forty five (45) adult male albino mice were caged into six groups. Mice were injected, i.p, 300 mg HY/kg and/or 0.67 mg CdCl2/kg b.w for 96 h, separately and alternated. The alternated trials were continued for consecutive eight days. Results show...

Author(s): Asmaa Shahrour, Mohamed Zowail and Khaled Sharafeldin

Shelf life improvement of sorghum beer (pito) through the addition of Moringa oleifera and pasteurization

November 2016

Pito is a traditional alcoholic beverage that is mostly brewed in the three northern regions of Ghana. Although widely consumed and used in many festivities, poor storability limits its economic potential as an income-generating venture for most women. This study was carried out to improve the shelf-life of pito through the addition of Moringa oleifera leaf extract and pasteurization (75 to 80°C). Microbial...

Author(s): Florence Adwoa Ayirezang, Courage Kosi Setsoafia Saba, Francis Kweku Amagloh and Hellie Gonu

Characterization of mutant cowpea [Vigna unguiculata (L) Walp] lines using random amplified polymorphic DNAs (RAPDs) and amplified fragment length polymorphism (AFLP) markers

November 2016

Phylogenetic relationship and polymorphism was detected in 10 cowpea lines comprising of leaf, flower and stem mutants, their putative parents and an exotic accession using 10 random amplified polymorphic DNAs (RAPDs) and three primer combinations of amplified fragment length polymorphism (AFLP) markers. These mutants were earlier obtained through the probable activities of transposable elements. The RAPD and AFLP...

Author(s): Olufisayo Atinuke Kolade, Mercy Oluremi Olowolafe and Iyiola Fawole

Pan-genome analysis of Senegalese and Gambian strains of Bacillus anthracis

November 2016

Bacillus anthracis is the causative agent of anthrax, and it is classified as “category A” biological weapon. There were six available complete genomes (A0248, Ames, Ames Ancestor, CDC684, H0491 and Sterne). Here, one Gambian and two Senegalese strains (Gmb1, Sen2Col2 and Sen3) were added. In this work, the pan-genome of B. anthracis was studied based on nine strains and using bioinformatics tools as Cluster...

Author(s): M. Mbengue, F. T. Lo, A. A. Diallo,  Y. S. Ndiaye, M. Diouf and M. Ndiaye

RNAi-based silencing of genes encoding the vacuolar-ATPase subunits a and c in pink bollworm (Pectinophora gossypiella)

November 2016

RNA interference is a post- transcriptional gene regulation mechanism that is predominantly found in eukaryotic organisms. RNAi demonstrated a successful gene silencing in insects that led to the development of novel approaches for insect pest management. In the current study, genes encoding vacuolar ATPase (V-ATPase) subunits a and c from the midgut of pink bollworm, Pectinophora gossypiella, were cloned and sequenced....

Author(s): Ahmed M. A. Mohammed

Genetic analysis of body weight of Takifugu rubripes at different developmental stages

November 2016

To elucidate the genetic mechanism of growth trait in Takifugu rubripes during ontogeny, developmental genetic analysis of body weight was conducted by mixed genetic model with additive-dominance effects, using complete diallel cross with three different strains of T. rubripes from Laizhou Shandong, Tangshan Hebei and Dalian Liaoning. Unconditional genetic analysis revealed that the unconditional dominance effects with...

Author(s): Aijun Ma,, and Xin’an Wang,,

Diversity of growth hormone gene and its relation with average daily gain in Simmental cattle in West Sumatera Province, Indonesia

November 2016

This study was aimed to analyse the genetic polymorphism of Growth Hormone (GH) polymorphism of Simmental cattle using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and its relation to average daily gain. The research was conducted in the Padang Mangatas Breeding Centre, Limapuluh Kota district, West Sumatera Province and Biotechnology Laboratory of Faculty of Animal Husbandry,...

Author(s): Hary Suhada, Sarbaini Anwar, Arnim, Hendri Maulana and Dan Yurnalis

Hesperidin effects on behavior and locomotor activity of diabetic Wistar rat

November 2016

Today, there are sufficient validated scientific data that support the existence of relations between diabetes and certain neuropsychiatric disorders, such as behavioral disorders, anxiety, cognitive decline and depression. The objective of this work was to investigate the effect of a natural bioflavonoid, the antioxidant hesperidin on the neuro behavioral alterations and locomotor function in streptozotocine diabetic...

Author(s): Doria Amina Bensaoula, Nadia Boukhris and Abdelkrim Tahraoui

Optimization of humidifying procedure in controlled environment for indoor cultivation of Pleurotus pulmonarius

November 2016

This study investigated mushroom cultivation in controlled indoor environment. For this, the environmental factors including humidity and temperature were controlled by humidifier and ventilation.  Four ventilations were installed on tops to bring the air from inside to outside and four small ventilations were installed on side wall along with black filter to bring the air from outside to inside. Four humidifiers...

Author(s): Tariqul Islam, Zarina Zakaria, Nasrul Hamidin and Mohd Azlan Bin Mohd Ishak

Molecular epidemiology of foot and mouth disease, bluetongue and pest de petites ruminants in Algeria: Historical perspective, diagnosis and control

November 2016

Molecular tools have become an increasingly important part of studying the epidemiology of infectious agents. These tools have allowed the aetiological agent within a population to be diagnosed rapidly with a greater degree of efficiency and accuracy than conventional diagnostic tools. They have enhanced understanding into the pathogenicity and virulence of the aetiological agent and subsequent deployment of appropriate...

Author(s): Moustafa KARDJADJ and Pam Dachung LUKA

Chlorophyll, nitrogen and antioxidant activities in Cumaru (Dipteryx odorata (Aubl.) Willd) (Fabaceae) in two water regimes

November 2016

The Cumaru (Dipteryx odorata (Aubl.) Willd.) is a species used by traditional populations and industries using timber and non-timber forest products. This study aimed to analyze the levels of chlorophyll A, B, total ammonia levels, nitrate, proline, electrolyte leakage and activity of oxidative enzymes in evaluation to tolerance of cumaru plants subjected to drought for 21 days of stress. The experiment was conducted in...

Author(s): Bruno Moitinho Maltarolo, Ellen Gleyce da Silva Lima, Vitor Resende do Nascimento, Kerolém Prícila Sousa Cardoso, Ana Ecídia de Araújo Brito, Tamires Borges de Oliveira, Glauco André dos Santos Nogueira, Karollyne Renata Souza Silva, Wander Luiz da Silva Ataíde, Cândido Ferreira de Oliveira Neto, Waldemar Viana De Andrade Junior and Benedito Gomes dos Santos Filho

Enhancement of yield and nutritional value of soybean sprouts by persimmon fruit powder

November 2016

Large amount of soybean sprouts has been produced for human consumption in Korea for a long time. Various techniques and production methods have been implied to enhance the quality and yield of soybean sprouts. The objective of the present study was to investigate the effect of persimmon fruit powder treatment on yield and nutritional value of soybean sprouts. The sprout yield was increased by 16% with the addition of...

Author(s): Il-Doo Kim, Sanjeev Kumar Dhungana, Jeong-Ho Kim, Hong Ahn, Hye-Ryun Kim and Dong-Hyun Shin

In vitro direct organogenesis in response to floral reversion in lily

November 2016

Our previous study indicated that the tiger lily (Lilium lancifolium var. Flore Pleno) has a great ability to produce inflorescence bulbils in nature as a form of natural phenomenon of floral reversion in plants. This present research was carried out to investigate the artificial floral reversion in in vitro culture of two lilies (Asiatic hybrid cv.”Black out"), and (Lilium longiflorum cv "White...

Author(s): Asker H. M.

Genetic diversity and population structure of maize landraces from Côte d’Ivoire

November 2016

Maize (Zea mays L.) occupies an important place among food crops in Côte d’Ivoire. However, no study on the genetic diversity of the species has been performed to date. This study aims at analyzing the diversity and genetic structure of 35 maize accessions using 10 microsatellite markers. These accessions are from different agro-ecological zones representative of the Ivorian territory. The results showed...

Author(s): N’DA Hugues Annicet, AKANVOU Louise, POKOU N’da Désiré, AKANZA Kouadjo Paul, KOUAKOU Charles Konan and ZORO BI Irié Arsène

Molecular genetic diversity in a core of cocoa (Theobroma cacao L.) clones with potential for selection of disease resistance, plant height and fruit production

November 2016

This study aimed to assess the genetic variability in groups of 11 clones of Theobroma cacao L., from different geographical regions, based on microsatellite markers, with the interest to characterize germplasm for breeding. The products of the amplification of these materials with 15 simple sequence repeat (SSR) markers were separated into ABI377 sequencer. The genotype encoded data were analyzed by means of...

Author(s): Adeilson Batista Lins, Pabliane Ramos Lawinscky, Antônia Marlene Magalhães Barbosa, Fernanda Amato Gaiotto and Ronan Xavier Corrêa

Agronomic evaluation and quality characteristics of three Cucurbitaceae varieties acclimated in Benin

November 2016

The present study aimed to evaluate some agronomical, physical and nutritional characteristics of seeds of three species of Cucurbitaceous, namely Citrulus lanatus, Lagenaria siceraria and Cucumeropsis edulus. Agronomic experiments were investigated over a two-year period during which, some agronomic performances of the tested varieties were evaluated. The physical characteristics of the collected fruits were also...

Author(s): Adam Salifou, Cherif Alidou, Fidèle P. Tchobo, Bruno Barea and Mohamed M. Soumanou

Effect of salt stress on growth and contents of organic and inorganic compounds in noni (Morinda citrifolia L.)

October 2016

Salinity is one of the abiotic stresses that most affect agricultural production, especially in arid and semi-arid regions; however, among species there are large differences in salt tolerance. In this study, the effects of salinity on the growth and accumulation of organic and inorganic solutes were evaluated in ‘noni’ seedlings at 1, 10, 20, 30 and 40 days of salt stress in a 5 x 2 completely randomized...

Author(s): Alide Mitsue Watanabe Cova, André Dias de Azevedo Neto, Rogério Ferreira Ribas, Hans Raj Gheyi and Renata Velasques Menezes

Effect of nitrogen and potassium fertilization on morpho-agronomic traits of three elephant grass (Pennisetum purpureum Schum.) genotypes for biomass production

October 2016

Elephant grass has been proposed for the energy sector as a possible source of renewable energy, because of its high biomass production. The aim of this study was to evaluate the effect of the mineral nutrients nitrogen and potassium on the morpho-agronomic traits (dry mater yield (DMY), percentage of DM (%DM), number of tillers per linear meter (NT), plant height (PH), stem diameter (SD) and leaf blade width (LW)) in...

Author(s): Antonio Alonso Cecon Novo, Rogério Figueiredo Daher, Geraldo de Amaral Gravina, Ernany Santos Costa, Juares Ogliari, Kleberson Cordeiro Araújo, Bruna Rafaela da Silva Menezes, Niraldo José Ponciano, Érik da Silva Oliveira and Verônica Britos Silva

Study of associative effects of date palm leaves mixed with Aristida pungens and Astragalus gombiformis on the aptitudes of ruminal microbiota in small ruminants

October 2016

The objectives of this trial were to evaluate interactions between microbial degradation of three substrates: date palm leaves as tannin-rich substrate, Astragalus gombiformis (as nitrogen source) and Aristida pungens (as carbon source). The model included the principal effects of single substrate and mixtures of multiple interactions. The forages were fermented alone or mixed with date palm leaves in various...

Author(s): Deffairi Djamila and Arhab Rabah

Isolation and characterization of cellulolytic Bacillus licheniformis from compost

October 2016

Eight cellulose degrading bacteria were isolated from compost and were identified as Bacillus licheniformis by 16S rRNA sequencing. Among the eight isolates, Bacillus licheniformis B4, B7 and B8 showed the highest cellulase activity. B. licheniformis B4 and B8 showed the maximum cellulase activity during the stationary phase of growth; but for B7, the maximum activity of cellulase was observed during the log phase....

Author(s): Nallusamy Sivakumar, Amira Al Zadjali, Saif Al Bahry, Abdulkhadir Elshafie and Elsadig Abdulla Eltayeb

Drought tolerant tropical maize (Zea mays L.) developed through genetic transformation with isopentenyltransferase gene

October 2016

Maize is a staple food crop for millions of Africans. Despite this fact, African farmers have been harvesting average grain yield of not more than 2 t/ha while there is a potential of producing more than 10 t/ha. Drought is one of the major abiotic constraints contributing to this low productivity. Drought diminishes crop productivity mainly by causing premature leaf senescence. The ipt gene codes for...

Author(s): Leta Tulu Bedada, Miccah Songelael Seth, Steven Maina Runo, Wondyifraw Teffera, Charless Mugoya, Clet Wandui Masiga, Richard Okoth Oduor, Eduardo Blumewald and Francis Wachira

Transcriptional modulation of genes encoding nitrate reductase in maize (Zea mays) grown under aluminum toxicity

October 2016

The free aluminum (Al) content in soil can reach levels that are toxic to plants, and this has frequently limited increased productivity of cultures. Four genes encoding nitrate reductase (NR) were identified, named ZmNR1–4. With the aim of evaluating NR activity and the transcriptional modulation of the ZmNR1, ZmNR2, ZmNR3, and ZmNR4 genes in leaves, 30-day-old hybrid maize BRAS 3010 plants were irrigated with a...

Author(s): Talita Cantú, Crislaine Emidio Vieira, Rafaélla David Piffer, Giovanna Carneiro Luiz and Silvia Graciele Hülse de Souza

Alternative tube caps on in vitro growth of Orbignya oleifera Burret.: An Arecaceae native cerrado domain

October 2016

The babassu (Orbignya oleifera Burret) is a palm that contains oilseeds whose biomass can be used for biofuel production. Due to difficulties in germinating the species, in vitro cultivation techniques are sought as a viable alternative to expand studies on germination. The objective of this study was to evaluate different types of tube caps for in vitro growth of babassu seedlings. Thus, zygotic embryos were inoculated...

Author(s): Mariluza Silva LEITE, Paula Sperotto Alberto FARIA, Flávia Dionísio PEREIRA, Fabiano Guimarães SILVA, Aurélio Rúbio NETO and João das Graças SANTANA

On-line methanol sensor system development for recombinant human serum albumin production by Pichia pastoris

October 2016

An on-line methanol sensor system was developed using a methanol probe, methanol sensor unit and peristaltic pump. The system was commanded using data acquisition (DAQ) and LabVIEW software. Calibration of the methanol sensor system was done in a medium environment with yeast cells during cells adaptation to methanol metabolism after glycerol feeding was stopped. The correlation equations between voltage output signal...

Author(s): Panchiga Chongchittapiban, Jӧrgen Borg, Yaowapha Waiprib, Jindarat Pimsamarn and Anan Tongta

Cloning and expression analysis of alcohol dehydrogenase (Adh) hybrid promoter isolated from Zea mays

October 2016

Hybrid promoters are created by shuffling of DNA fragments while keeping intact regulatory regions crucial of promoter activity. Two fragments of alcohol dehydrogenase (Adh) promoter from Zea mays were selected to generate hybrid promoter. Sequence analysis of both alcohol dehydrogenase promoter fragments through bioinformatics tools identified several crucial cis regulatory elements and transcription factors binding...

Author(s): Ammara Masood, Nadia Iqbal, Hira Mubeen, Rubab Zahra Naqvi, Asia Khatoon and Aftab Bashir

Production and characterization of endoglucanase secreted by Streptomyces capoamus isolated from Caatinga

October 2016

Cellulases are hydrolases of great importance to industries, especially due to their ability to produce ethanol via hydrolysis of cellulolytic materials. Actinomycetes are the producers of these enzymes, particularly the genus Streptomyces sp. The present study is the first report on the production and characterization of cellulolytic complex secreted by Streptomyces capoamus, isolated from the rhizosphere soil of...

Author(s): Rafael Lopes e Oliveira, Camila Beatriz Atanásio Borba, Sergio Duvoisin Junior, Patricia Melchionna Albuquerque, Gláucia Manoella de Souza Lima, Norma Buarque de Gusmão, Edmar Vaz de Andrade and Leonor Alves de Oliveira da Silva,

SIM2PeD– Intelligent monitoring system for prevention of diabetic foot

October 2016

Diabetes is an endocrine chronic disease that causes high blood sugar level, produced by retardation in, or deficiency of, glucose metabolism in the body of the individual with the disease. Neuropathies and/or angiopathies are complications of diabetes that result in changes in the lower limbs which subsequently evolve for the diabetic foot. Diabetic foot represents one of the most devastating complications of diabetes...

Author(s): Rodrigo Medeiros, Cicília Raquel Maia Leite, Suélia de Siqueira Rodrigues Fleury Rosa, Marina Pinheiro,  Luísiane de Ávila Santana and Jane Dulius

Lipase production from a wild (LPF-5) and a mutant (HN1) strain of Aspergillus niger

October 2016

In this study, a wild (LPF-5) and a mutant (HN1) strain of A. niger were compared for lipase production. Several physical parameters (carbon source, nitrogen source, pH, temperature and incubation period) were optimized for maximization of lipase production. Lipase activity between wild type and mutant strain were compared. Among all carbon sources, mixture of glucose (1%, w/v) and olive oil (1%, v/v) exhibited maximum...

Author(s): Arun Kumar Sharma, Vinay Sharma, Jyoti Saxena and Arindam Kuila

Comparison of protease production from newly isolated bacterial strains T5, T3 and H3 after immobilization

October 2016

Protease has gained a very important position in many industries such as food, pharmaceutical, chemical and leather industries. In this research, protease was obtained from bacteria. The bacterial strain was obtained from soil which was collected from different areas of Lahore, Pakistan. Fermentation medium (by using sub-merged fermentation technique) was incubated for 48 h at 37°C temperature and agitation speed of...

Author(s): Mina Ilyas

DNA markers reveal genetic structure and localized diversity of Ethiopian sorghum landraces

October 2016

North Eastern Ethiopia is a major sorghum-growing region. A total of 415 sorghum landraces were sampled to represent the range of agro-ecologies (three altitude ranges) as well as spatial heterogeneity, that is, 4 zones: North Welo, South Welo, Oromiya and North Shewa with each zone containing 2 to 5 districts. The landraces were genotyped with simple sequence repeats (SSR) and inter simple sequence repeats (ISSR)...

Author(s): Haile Desmae, David R. Jordan and Ian D. Godwin,

Molecular screening of rice (Oryza sativa L.) germplasm for Xa4, xa5 and Xa21 bacterial leaf blight (BLB) resistant genes using linked marker approach

October 2016

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has now become one of major rice diseases. During recent years, its occurrence has increased in Pakistan, particularly in ‘Kallar tract’ which is well-known for quality rice cultivation. Development of new resistant varieties by utilizing the available resistant gene-pool is most reliable breeding technique for the plant breeders. The...

Author(s): Muhammad Sabar, Tahira Bibi, Hafiz Umar Farooq, Zulqarnain Haider, Imad Naseem, Abid Mahmood and Muhammad Akhter

Drying kinetics of Baru almond (Dipteryx alata Vog) used for the production of biodiesel

October 2016

This study aimed to evaluate the drying kinetics of Baru (Dipteryx alata Vog), fit different mathematical models to the experimental values as a function of the water content, determine the diffusion coefficient and energy of Baru almonds during the drying process. Baru almonds were dried at 50, 60 and 70°C temperatures in a tray dryer until the water content reached 13% b.u. A non-linear regression analysis was...

Author(s): Paulo Cleber Mendonça Teixeira and Abraham Damian Giraldo Zuniga

Renal and hepatic histopathology of intraperitoneally administered potassium permanganate on Clarias gariepinus juveniles

October 2016

The biochemistry and histopathology of intraperitoneally administered potassium permanganate was investigated in Clarias gariepinus. Acute toxicity of the KMnO4 was determined by intraperitoneally injecting the fish with 0.0, 1.5, 2.0, 4.0 and 6.0 mg/kg. The 96 h lethal concentration (LC50) value obtained from the intraperitoneal injection of the juveniles of C. gariepinus with KMnO­4 was 2.001 mg/kg KMnO4 at 95%...

Author(s): Odo Gregory Ejikeme, Agwu Ekenma Juliana, Eke Felicia and Ikele Chika Bright and Ezea Celestine Osita

Development of ethanol production from cooking oil glycerol waste by mutant Enterobacter aerogenes

October 2016

Bacterial strains, capable of producing ethanol by using cooking oil glycerol waste (derived from biodiesel production) as sole C-source were screened from soil samples. Based on 16S rRNA sequence, the selected strain was identified as Enterobacter aerogenes G2WG. The sequence was submitted to GenBank and the accession number KU315428 was obtained. The genetic nature of the wild type strain was altered for enhancing...

Author(s): Thalisa Yuwa-amornpitak and Kannika Chookietwatana

Production, characterisation and flocculation mechanism of bioflocculant TMT-1 from marine Bacillus pumilus JX860616

October 2016

Bioflocculant from marine Bacillus pumilus JX860616 was characterised and its flocculation mechanism determined. The bacterium was identified by 16S rRNA and the bioflocculant was obtained through solvent extraction after optimum medium composition and culture conditions were established. The physicochemical analysis of the bioflocculant were obtained by scanning electron microscopic (SEM) equipped with elemental...

Author(s): Maliehe, T. S., Simonis, J., Basson, A. K., Reve, M., Ngema, S. and Xaba, P. S.

In vitro regeneration of disease free enset [Ensete ventricosum (Welw) Cheesman] planting materials from bacterial wilt diseased plants using shoot tip culture

October 2016

Enset is an important food crop produced in Ethiopia with great role in food security. The demand of the crop is increasing throughout the country. However, the production as a whole is decreasing due to devastation by enset bacterial wilt. The studies were conducted to develop the procedure for obtaining multiple disease free plantlets from infected enset plants. Shoot tip explants from infected suckers of three clones...

Author(s): Genene Gezahegn and Firew Mekbib

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