African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

AJB Articles

Spectrophotometric method for quantification of soil microbial biomass carbon

April 2016

The search for more suitable alternatives in analytical processes is a strategy to reduce environmental damages. The present study aimed to quantify the soil microbial biomass carbon (SMB-C), establishing a comparison between the methods involving quantification of SMB-C by titration of the samples with ferrous sulfate ammonia and molecular absorption spectrophotometry methods. This comparison was performed by two soil...

Author(s): Alessandra Oliveira da Silva, William Marra Silva, Carlos Hissao Kurihara and Fábio Martins Mercante

Prospects of genetic modified maize crop in Africa

April 2016

Genetic modified maize crop increases annually as a result of food insecurity and limited land caused by rapid population increase of over seven billion in the world. Scientists have been playing their role to address this food insecurity problem. The use of genetically modified (GM) maize crop to feed people is one of the proposed ways, because it yields more compared to the conventional varieties. However, there are...

Author(s): Andekelile Mwamahonje and Leon Mrosso

Genome-wide analysis of mechanosensitive channel of small conductance (MscS)-like gene family in common bean

April 2016

Mechanosensitive (MS) ion channels are transmembrane proteins that open and close in response to mechanical forces produced by osmotic pressure, sound, touch and gravity. In plants, MS have an important role in different biological processes like gravity detection, maintenance of plastid shape and size, lateral root emergence, growth of pollen tube, and plant-pathogen interactions. In this study, homologous...

Author(s): Silvia Graciele Hülse de Souza, Adriana Pereira da Silva, Tania Mayumi Ito, Eduardo Goiano da Silva, and Talita Cantú

Biochemical effects of ethylene diamine tetra-acetic acid (EDTA) on cadmium treated maize (Zea mays L.) and cowpea (Vigna unguiculata L.)

April 2016

The effects of ethylenediaminetetra-acetic acid (EDTA) on germination, length of stem, area of leaf, fresh weight, level of lipid per oxidation, alkaline phosphatase, acid phosphatase, super oxide dismutase (SOD) and catalase in the roots of cadmium (Cd) treated maize (Zea mays L) and cowpea (Vigna unguiculata L) seedlings after 7 and 21 days of germination were determined in this study. The results obtained, indicate...

Author(s): Emeke E. Agbadah, Kenneth C. Nwachukwu and Michael P. Okoh

Effect of monosodium glutamate and aspartame on behavioral and biochemical parameters of male albino mice

April 2016

The present study aimed to investigate the individual and combined effect of mono-sodium glutamate (MSG) and aspartame (ASM) on biochemical, blood parameters and neuro-behavioral aspects of mice. The results indicated that exposure induced many changes in fear and anxiety behavior. The non-social and social behavior of the exposed mice was significantly affected, showing an increase in the former and a decrease in the...

Author(s): Gasem Mohammad Abu-Taweel

In vitro antimicrobial properties of friedelan-3-one from Pterocarpus santalinoides L’Herit, ex Dc

April 2016

Chemical investigation of ethyl acetate leaf-extract of Pterocarpus santalinoides led to isolation of a friedelane-terpenoid. Structure elucidation was done by comparison of the Nuclear Magnetic Resonance (NMR) spectral features with literature data and was consistent with reported values for friedelan-3-one. The following microorganisms (Clinical isolates): methicillin resistant Staphylococcus aureus (MRSA), S. aureus,...

Author(s): Ichiko Chic Odeh, Terrumun Amom Tor-Anyiin, John Ogbaji Igoli and John Vershima Anyam

Nanoformulation and antibiotic releasing property of cefotaxime nanoparticles

April 2016

The objective of this study was to design nano-antibiotic to enhance their release from biomaterial agents. Cefotaxime was used as a model antibiotic substance in this carrier system. These nanoparticles were preformulated using different concentrations of polycaprolactone (PCL) and poly (vinyl alcohol) as coating material and prepared using double emulsion solvent evaporation method. The physiochemical properties...

Author(s): Maysaa Chasib Al-Moahmmedawi

Polymorphism of growth hormone gene and its association with wool traits in Egyptian sheep breeds

April 2016

Growth hormone (GH) gene has been described as a candidate gene for marker-assisted selection in different farm animals. The present study was designed to identify the polymorphism in GH gene and its association with variation of wool traits in Egyptian sheep breeds. Wool and blood samples were collected from 42 animals including two breeds (Barki and Rahmani) and one crossbred (Rahmani x Awase). Measurements of wool...

Author(s): Ibrahim M. Farag, Ahmed M. Darwish, Hassan R. Darwish, AbdelAziz K. B., Ramadan W. A., Mohamed M.I. and Othman E. Othman

Effects of diets containing Cissus rotundifolia flour on lipid profile of rats and postprandial glucose levels of normoglycemic human adults

April 2016

This study evaluated the effect of Cissus rotundifolia (CR), on the lipid profile of rats and postprandial blood glucose level of normoglycemic human adults. CR flour was processed using traditional Nigerian method of processing. The animal experiment involved male Sprague-Dawley rats fed for 14 days with hypercholesterolemic diet of corn oil, cholesterol, casein, mineral mix, vitamin mix, sucrose, solka floc and corn...

Author(s): Uchenna Agatha Onyechi and Vivienne Nkiruka Ibeanu

Possibilities of sweet potato [Ipomoea batatas (L.) Lam] value chain upgrading as revealed by physico-chemical composition of ten elites landraces of Benin

March 2016

Sweet potato is one of the most important food security promoted root crops in the world, especially in sub-Saharan Africa. Unfortunately, the crop is still neglected and underutilized in Benin Republic. To establish baseline data for its better utilization for upgrading its value chain, 10 selected local varieties (01 cream, 02 white, 03 yellow, and 04 orange flesh-colored) were compared for their macro-nutritional...

Author(s): Sanoussi A. F., Dansi A., Ahissou H., Adebowale A., Sanni L. O., Orobiyi A., Dansi M., Azokpota P. and Sanni A.

Effect of osmotic stress on in vitro propagation of Musa sp. (Malbhog variety)

March 2016

In vitro propagation of banana preferably use sword sucker as explant source where microbial contamination poses a great problem in establishment of aseptic cultures. This study demonstrates up to 36% reduced microbial contamination in aseptic culture establishment and subsequent micropropagation due to osmotic stress induction in the banana suckers. Osmotic stress was induced by keeping the freshly collected suckers in...

Author(s): Swapan Kumar Sinha and Alak Chandra Deka

Development of putative transgenic lines of cassava variety H-226

March 2016

Cassava production in India is drastically affected by cassava mosaic disease (CMD) caused by the Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV). An attempt was done to develop transgenic cassava lines resistant to SLCMV through RNAi vector targeting a conserved 440 bp of 5’ end of SLCMV Rep (AC1) gene which also overlaps with part of AC4 gene, and functions as a viral RNAi...

Author(s): Anuradha Arun, Palanisami Prabha, Krish K. Kumar and Ponnusammi Balasubramanian

Determining water content and other impurities in Siparuna guianensis Aublet essential oil using differential scanning calorimetry

March 2016

Siparuna guianensis Aublet is a predominant species in the Brazilian Cerrado. Some studies found that its essential oil has properties that could be useful for manufacturing new products. Its quality depends on physicochemical properties, since its degradation, as well as water content and other volatile materials may cause several changes in its features. The differential scanning calorimetry (DSC) was used to...

Author(s): Augustus Caeser Franke Portella, Marilda Munaro, Chrystian de Assis Siqueira, Kleber Franke Portella, Magnólia de Mendonca Lopes, Alex Sander Rodrigues Cangussu and Raimundo Wagner de Souza Aguiar

Antioxidant activity, phenolic and flavonoid contents of some wild medicinal plants in southeastern Algeria

March 2016

This study reported on five plants known for their use in traditional medicine in southeastern Algeria, namely: Artemisia campestris, Asteriscus pygmaeus, Pituranthos chlorantus, Pallenis spinosa and Aizoon hispanicum. The work aimed to determine the antioxidant activity, phenol and flavonoid contents of their aqueous and methanolic extracts. Flavonoids and phenol contents varied according to the nature of the extract...

Author(s): Abdelkrim Khettaf, Nacera Belloula and Seloua Dridi

Genetic diversity and gene flow revealed by microsatellite DNA markers in some accessions of African Plum (Dacryodes edulis) in Cameroon

March 2016

Dacryodes edulis is a multipurpose tree integrated in the cropping system of Central African region still dominated by subsistence agriculture. Some populations grown are wild which can provide information on the domestication process, and could also represent a potential source of gene flow. Leaves samples for DNA extraction were collected from wild forms in Mbakwa supe region and from cultivated forms in Yaounde and...

Author(s): Nehemie Donfagsiteli Tchinda, Bramwel Waswa Wanjala, Alice Muchugi, Fotso, Germo Nzweundji Denis Omokolo Ndoumou and Robert Skilton

Estimate of genetic diversity in cassutinga (Croton heliotropiifolius) based on molecular markers

March 2016

The Croton heliotropiifolius (Euphorbiaceae family) is a shrubby plant that has attracted attention both by the need for conservation as for their pharmacological potential. The present study aimed to characterize the diversity and structure genetic of a population of C. heliotropiifolius present in a forest fragment in the city of Itapetinga, Bahia. Young leaves were collected from 41 individuals after DNA extraction...

Author(s): Tallita de Oliveira Rocha, Janaina Silva Freitas, Elisa Susilene Lisboa dos Santos, Murilo Marques Scaldaferri, Claudine Gonçalves de Oliveira and Carlos Bernard Moreno Cerqueira-Silva,

In vitro screening of selected essential oils from medicinal plants acclimated to Benin for their effects on methane production from rumen microbial fermentation

March 2016

Enteric methane production lowers the efficiency of feed utilization in ruminants and contributes to greenhouse gas emissions which are responsible for global climate change. This study examined the effects of nine essential oils (EO) from Citrus aurantifolia, Cymbopogon citratus, Eucalyptus citriodora, Laurus nobilis, Lippia multiflora, Mentha piperita, Ocimum basilicum, Ocimum gratissimum and Zingiber officinalis on...

Author(s): Jacques B. Kouazounde, Long Jin, Tim A. McAllister and Joachim D. Gbenou

Nutritional composition of bioproducts generated from semi-solid fermentation of pineapple peel by edible mushrooms

March 2016

Mushroom production is a very efficient alternative to agro-industrial residues recycling. The proximal composition, content of macro and micro minerals, amino acid and proteolytic activity were assessed in the pineapple peel and in the bioproducts generated from pineapple peel and mycelium of Pleurotus albidus, Lentinus citrinus and Pleurotus florida. The bioproducts were obtained by semi-solid fermentation and drying...

Author(s): Raiane Áila Teixeira Souza, Tamiris Rio Branco da Fonseca, Larissa de Souza Kirsch, Larissa Svetlana Cavalcante Silva, Mircella Marialva Alecrim, Raimundo Felipe da Cruz Filho and Maria Francisca Simas Teixeira

Histopathological changes in female rabbits administered with aqueous extract of Calotropis procera

March 2016

Calotropis procera is an evergreen perennial shrub, which is found mainly in the arid regions and produces copious latex when cut. It has been reported to possess medicinal properties but equally pose deleterious effect in animals. In a bid to exploit its pharmacological properties, it was necessary to ascertain its level of safety. A toxicological evaluation of the aqueous extract of fresh leaves of the plant was...

Author(s): Jato, D. M., Jacob, K. A., Zainab, A., Okewole, P., Atiku, A., Shamaki, D., Umar, M. B. and Chudy, N.

Effect of osmotic stress on in vitro propagation of Musa sp. (Malbhog variety)

March 2016

In vitro propagation of banana preferably use sword sucker as explant source where microbial contamination poses a great problem in establishment of aseptic cultures. This study demonstrates up to 36% reduced microbial contamination in aseptic culture establishment and subsequent micropropagation due to osmotic stress induction in the banana suckers. Osmotic stress was induced by keeping the freshly collected suckers in...

Author(s): Swapan Kumar Sinha and Alak Chandra Deka

In vitro regenerative potentials of the medicinal plant Abutilon indicum (L.) Sweet

March 2016

The present study was undertaken to evaluate the most suitable concentration of growth regulators for callus induction and subsequent organogenesis in Abutilon indium (L.) Sweet (Malvaceae) leaf, stem and seed explants. Since the plant has a lot of medicinal importance, it was subjected to culture in vitro. Kinetin for seeds, 2,4-dichlorophenoxyacetic acid (2,4-D), benzyl amino purine (BAP), naphthalene acetic acid...

Author(s): Sudarshana Mysore Shankarsingh, Nissar Ahmad Reshi and Girish Hodiyala Vasanaika

Glyoxalase I expression pattern in Hevea brasiliensis seedlings under varied stress conditions

March 2016

Drought is one of the most important stress factors which adversely affect plants’ growth and productivity. Global climate change may make this situation more serious in the years ahead.  Considering the long time span required for the generation of drought resistant genotypes in Rubber (Hevea brasiliensis) through conventional breeding, molecular interventions to engineer plants to have either drought...

Author(s): Siraj M. V. P, Thulaseedharan, A. and Anu Augustine

Nutritional food content of seed and effects of five different growing media on the seed germination and seedling growth of Afzelia africana SM Caesalpiniaceae

March 2016

Nutritional food content of seed and effect of five different growing media: Top forest soil (TS), sawdust (SD), 2:1 mixture of; TS+SD, TS+composted poultry manure (TS+PM) and 2:1:1 mixture of TS+SD+PM on the seed germination and seedling growth of Afzelia Africana SM Caesalpiniaceae  were investigated. Thirteen chemical food nutrients were detected in the species seed. These occurred in varying percentages. The...

Author(s): Nzekwe, U., Ajuziogu, G. C., Njoku, E. U., Okafor, G. I., Sani, M. B., Onyeke, C. C.­­­­, Onyeonagu, C. C., Ohaneje, A. A. and Eze, C. S.

Response of African eggplants to Fusarium spp. and identification of sources of resistance

March 2016

Eggplant (Solanum spp.) production in Arumeru district and other parts of Africa is severely affected by wilting diseases of unknown etiology. Fusarium spp. characterized through morphological and sequence analysis of the translation elongation factor associated with Fusarium wilt of eggplants was used to test the response of three different eggplant species. Three Solanum spp. accessions were tested in a screen house...

Author(s): Phoebe Kirigo Mwaniki, Mathew Musumbale Abang,#, Isabel Nyokabi Wagara#, Joseph Ngwela Wolukau and Schroers Hans-Josef#

A commercial micropropagation protocol for virupakshi (AAB) banana via apical meristem

March 2016

In vitro micropropagation of banana (Musa spp.) cv.virupakshi (Hillbanana) was studied. Suckers were collected from the germ plasm block of Jain R&D (originally established from the suckers from Palani Hills, Tamil Nadu) during summer. The sucker surface sterilized with 1% NaOCl for 30 min gave 100% survival without any contamination. Apical meristems that were isolated and cultured on MS based media supplemented...

Author(s): Karule P., Dalvi V., Kadu A., Chaudhari R., Subramaniam V. R., and Patil A. B.

Detection of extracellular enzymatic activity in microorganisms isolated from waste vegetable oil contaminated soil using plate methodologies

March 2016

In the present study, from a total of 100 strains isolated from waste vegetable oil contaminated soil, 38 bacterial and 14 fungi strains that presented positive lipolytic activity were obtained by detection through Rhodamine B Agar 0.02% w/v as a screening method. Additionally, two other enzymatic activities were determined. Positive proteolytic activity was evaluated in Casein Hydrolysis Agar and chitinolytic activity...

Author(s): Eugenia G. Ortiz Lechuga, Isela Quintero Zapata and Katiushka Arévalo Niño⃰

Production of thermophilic and acidophilic endoglucanases by mutant Trichoderma atroviride 102C1 using agro-industrial by-products

March 2016

Many traditional mutagenic strategies have been used to improve cellulase production by micro-organisms, especially fungi species. Trichoderma species are among cellulolytic fungi, those that have been most extensively studied, due to their efficient production of these enzymes. In the present study, N-methyl-N´-nitro-N-nitrosoguanidine (NTG) was used as mutagenic agent to obtain cellulolytic mutant from wild...

Author(s): Mariana Menezes Quadros de Oliveira, André Luiz Grigorevski de Lima, Elba Pinto da Silva Bon, Rosalie Reed Rodrigues Coelho and Rodrigo Pires do Nascimento

Genetic diversity and structure of goats within an early livestock dispersal area in Eastern North Africa

March 2016

In this study we genotyped 14 microsatellites to assess genetic diversity, population stratification and demographic dynamics using Egyptian local goats (Zaraibi, Baladi, Saidi and Barki) and the Shami (Damascus) goat from the Middle East and West Asia close to the geographic center of goat domestication. We observed high levels of allelic and genetic diversity that was partitioned into six gene pools. Cluster analyses...

Author(s): Elbeltagy A. R., Aboul-Naga A. M., Hassen H., Solouma G. M., Rischkowsky B. and Mwacharo J. M.

Histological and biochemical evaluations of the liver and kidney of Wistar rats fed with fish meal of Sarotherodon melanotheron captured with Tephrosia vogelii’s powder

March 2016

To evaluate the toxicity of fish captured with Tephrosia Vogelii (TV), an ichtyotoxic plant, the Wistar albino rats were fed with the flour of tilapias Sarotherodon melanotheron poisoned with TV leaves powder. This study aimed to evaluate the poisonous effects of TV on various organs of rats, especially the liver and kidney. Three groups of Wistar rats were constituted and fed for 28 days. Histological sections were...

Author(s): Dougnon Tossou Jacques, Kpodekon T. Marc, Houessionon M. Jedirfort and Kiki S. Pascal

Unexplored vegetal green synthesis of silver nanoparticles: A preliminary study with Corchorus olitorus Linn and Ipomea batatas (L.) Lam

March 2016

Antibacterial properties of silver ion are known from ancient times. The plant extract mediated synthesis of nanoparticles is gaining popularity due to green chemistry for the generation of nanosized materials. Corchorus olitorus Linn and Ipomea batatas (L.) Lam are world crops having leaves of high nutritional value. In the present work, Ag and AgCl nanoparticles were synthesized by incubating leaf aqueous extracts...

Author(s): François Eya’ane Meva, Marcelle Loretta Segnou, Cecile Okalla Ebongue, Agnes Antoinette Ntoumba, Djiopang Yadou Steve, Fanny Aimee Essombe Malolo, Lidwine Ngah, Harouna Massai and Emmanuel Mpondo Mpondo,

Effects of season on the yield and quality of agar from Gelidium sesquipedale (Rhodophyta) from Mostaganem, Algeria

March 2016

The aim of this work was to study the seasonal variations in the yield and physicochemical properties of agar of Gelidium sesquipedale. The algae were collected from January 2011 to December 2011, from the coast of Mostaganem, western Algeria. The overall results show that the dry biomass of Gelidium sesquipedale was constant throughout the year (22.34 ± 3.18 %), while the agar yield varied seasonally with...

Author(s): S. Nil, S. Ali-Mehidi, A. Zellal and S. M. E. A Abi-Ayad

Chemical compositions and antimicrobial activity of twig essential oils from three Xylopia (Annonaceae) species

March 2016

The chemical composition of twig essential oils of Xylopia malayana, Xylopia elliptica and Xylopia fusca were analyzed using GC and GCMS. Both X. malayana (12.9%) and X. fusca (11.8%) twig oils contained spathulenol as the major constituent while X. elliptica twig oil was found to be rich in terpinen-4-ol (11.9%). The antimicrobial activities of the essential oils were evaluated against six gram positive bacteria, two...

Author(s): Siti Humeirah Abdul Ghani, Nor Azah Mohamad Ali, Mailina Jamil, Mastura Mohtar, Saiful Azmi Johari, Mazurah Mohamad Isa and Mohd Faridz Zoll Patah

Effect of acaricidal activity of Solanum nigrum on Tetranychus urticae Koch under laboratory conditions

March 2016

Tetranychus urticae is an economically important pest for different commercial crops and is traditionally controlled with synthetic chemical products that are usually toxic. Therefore, alternative strategies are implemented to allow producers to meet the phytosanitary requirements demanded by quality seals. One of these alternatives is the use of plant extracts. The aim of this study was to determine the effect of...

Author(s): Stephanie Johana Numa Vergel, Lorena Rodríguez Coy, Daniel Rodríguez Caicedo and Ericsson Coy-Barrera

Histomorphometry aspect of thyroid gland and biochemical profile in pregnant and non-pregnant dromedary camels

March 2016

Modulation of body physiology and metabolism is a prerequisite for successful pregnancy in camels, which is about a year and few months. The present study was carried out to verify the effect of pregnancy on histomorphometry and functional traits of thyroid gland, as well as some biochemical indices of liver and kidney functions and mineral profile in one humped camels, under Egyptian climatic conditions or desert...

Author(s): Yousef F. Ahmed, Karima Gh. M. Mahmoud, Mohamed M. M. Kandiel, Nawito M. F. and AbdelRazik, A. M. A.

Nutritional status, lifestyle and knowledge of predisposing factors on hyperlipedemia among out-patients in Enugu State University Teaching Hospital (ESUTH), Nigeria

March 2016

The growing trend of hyperlipidemia in most developing countries has resulted largely from changes in lifestyle, diet and lack of adequate exercise which have led to decreased life expectancy and burden of cardiovascular diseases. A purposive sample size of 206 out-patients was selected for this study after obtaining their due consents. Out of these, 108 (52.84%) were female and 98 (47.6%) males. Validated structured...

Author(s): Nwamarah, Joy Ugo, Otitoju, Olawale and Otitoju, Grace Taiwo O.

Protective effect of Haloxylon salicornicum on hepatic and renal functions of Wistar rats exposed to aluminium

March 2016

Aluminium is present in some manufactured medicines and foods. It is known that aluminium causes oxidative stress. Therefore, the present study was undertaken to determine the effectiveness of Haloxylon salicornicum extract in modulating aluminium chloride (AlCl3) induced oxidative stress in rats. Male rats (40 to 50 g) were divided into four groups of six animals each. The experimental protocol was based on the...

Author(s): Fatima Zohra TAHARI, Monya LABLACK, Nadia AЇT HAMADOUCHE, Zineb TAHARI and Abdelkader AOUES

Histopathological effects of Cyperdicot and vitamin E supplementation on selected organs of Clarias gariepinus (Burchell, 1822) reared in a tropical fish farm in Nigeria

March 2016

This study conducted in 2014 investigated the histopathological effects of Cyperdicot and vitamin E supplementation on some selected organs in juveniles of Clarias gariepinus. Fish were exposed to 0. 0.08 and 0.16 mg/L Cyperdicot and vitamin E. Fish were divided into six groups: control, 0.80 mg/L; Cyperdicot, 0.16 mg/L; Cyperdicot, vitamin E, vitamin E + 0.08 mg/L Cyperdicot, and vitamin E + 0.16 m/L Cyperdicot...

Author(s): Odo, G. E., Agwu, J. E., Madu, J., Ezea, C. O., Nnamonu, E. I. and Eneje, V. O.

Cellulose degradation capabilities of dung beetle, Euoniticellus intermedius, larva gut consortia

March 2016

This study assessed the capabilities of the dung beetle, Euoniticellus intermedius (Coleoptera: Scarabaeida), larva gut consortia in degrading cellulose that can serve as glucose source for biofuels production. A total of 144 live dung beetles were randomly collected from a dairy farm and bred in a temperature controlled insect rearing room. On reaching the late second to third instar stage, dung beetle larvae were...

Author(s): Munamato Mabhegedhe, Karl Rumbold and Monde Ntwasa

HPLC quantification of phenolic content and assessment of methanolic extract of Antiaris africana for toxicological study

March 2016

The study was aimed at evaluating the toxicological and antioxidant activities of Antiaris africana Engl. (family Moraceae), that is used in Nigeria and other West Africa countries as a panacea for the treatment of several ailments. The methanolic extract of A. africana (MEA) obtained was analysed for antioxidant activities in vitro and screened for various phytochemicals present. Phenolic and flavonoid contents were...

Author(s): Omotayo B. Ilesanmi, Tolulope M. Olaleye, Afolabi C. Akinmoladun and Taiwo T. Alawode

Physical and chemical properties of the acid protease from Onopordum acanthium: Comparison between electrophoresis and HPLC of degradation casein profiles

March 2016

A protease was extracted by grinding, precipitation and gel filtration from Onopordum acanthium flowers. The physicochemical study of the enzyme showed an optimum pH of 4, a temperature of 40°C and kinetic parameters of 12.25 mM-1 for KM and 1329.6 UmL-1 for Vmax. The inhibition by pepstatin indicated that it is an aspartyl-protease (APs). Zymogram showed that the protease has a monomeric structure and a molecular...

Author(s): Malika Benkahoul, Meriem Benchiheub, Ines Bellil, Douadi Khelifi and Aicha Mechakra Maza

Antiproliferative activity of protein extracts from the black clam (Chione fluctifraga) on human cervical and breast cancer cell lines

February 2016

The wide diversity of the marine environment has been an important resource for the discovery of new bioactive agents from marine organisms. The aim of this study was to obtain protein extracts from the clam Chione fluctifraga and determine its antiproliferative activity against cervical and breast cancer cells. The extracts were obtained by ammonium sulfate fractionation, gel filtration and ion exchange chromatography....

Author(s): Hiesu García-Morales, Luis Enrique Gutiérrez-Millán, Miguel A.Valdez, Armando Burgos-Hernández, Teresa Gollas-Galván and María G. Burboa

Toxicity effects of ethanol extract of Simarouba versicolor on reproductive parameters in female Wistar rats

February 2016

Simarouba versicolor is popularly known as pau-paraiba. It belongs to the Simaroubaceae family and is found in the Northeast of São Paulo and in some parts of the states of Pará and Mato Grosso do Sul. It is known that this and other species from this genus have several activities: insecticides, anti-inflammatory, antitumor, anthelmintic, among others. The effects of ethanolic extract of the bark of S....

Author(s): Marlene S. Oliveira, Maria Zenaide L. C. M. Fernandes, Ana Lys B. B. Mineiro, Rosimeire F. dos Santos, Gregório E. N. Viana, Josana M. Coelho, Sahâmia M. Ribeiro, Ana Paula G. P. Cunha, Joselene F. Costa and Rozeverter M. Fernandes

Codon usage bias analysis for the coding sequences of Camellia sinensis and Brassica campestris

February 2016

Codon usage bias plays an important role in the regulation of gene expression. A couple of measures are widely used to quantify the codon usage in genes. On the other hand, no quantitative endeavour has been made to compare the pattern of codon usage diversity within and between different genes of Camellia sinensis and Brassica campestris. Nucleotide composition and its relationship with codon usage bias were analyzed....

Author(s): Prosenjit Paul and Supriyo Chakraborty

Identification of accelerated evolution in the metalloproteinase domain of snake venom metalloproteinase sequences (SVMPs) through comparative analysis

February 2016

Computational protein sequence analysis is one of the most important tools used for understanding the evolution of closely related proteins sequences including snake venom metalloproteinase sequences (SVMPs) which give valuable information regarding genetic variations. The fundamental objective of the present study is to screen the evolution distributed in metalloproteinase domain regions of protein sequences among...

Author(s): Mahmudul Islam, Zahida Yesmin Roly, Mirza Ruhullah, Zohorul Islam, Alsan Sharia, Rafsan Zani Tanvir and Khaja Foyasal

Genetic variability of sorghum landraces from lower Eastern Kenya based on simple sequence repeats (SSRs) markers

February 2016

The aim of this study was to estimate the genetic variability of sorghum landraces grown in lower eastern Kenya based on simple sequence repeats (SSRs) markers. A total of 44 landraces obtained from the farmers and four improved varieties were analyzed using 20 SSR markers. All markers were polymorphic with polymorphism information content (PIC) value ranging from 0.04 to 0.81 with a mean of 0.49. Heterozygosity ranged...

Author(s): Catherine W. Muui, Reuben M. Muasya, Duncan T. Kirubi, Steven M. Runo and Arthur Karugu

Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews)

February 2016

As an alternative to conventional lighting systems, light emitting diode (LED) has been demonstrated to be an artificial flexible lighting source for commercial micropropagation. The objective of this study was to determine the effects of different LED light quality on in vitro shoot proliferation and growth of Vanilla planifolia. To evaluate shoot proliferation, axillary bud cuttings (3 to 5 mm in diameter) of V....

Author(s): Jericó Jabín Bello-Bello, Eduardo Martínez-Estrada, José Humberto Caamal-Velázquez and Victorino Morales-Ramos

Detection of metallo-beta-lactamase producing Pseudomonas aeruginosa using a modified IMP-lysate assay

February 2016

Since the increasing prevalence of carbapenem-resistant Pseudomonas aeruginosa spp., accurate detection of metallo-β-lactamase (MBL) such as blaVIM type enzyme producing isolates became very important. However, phenotypic MBL detection methods previously reported are not highly sensitive or highly specific. This study aimed to evaluate the performance of a modified IMP-lysate test, the double-disk-synergy-test...

Author(s): Olfa Sioud, Mariem Nasri, Mahjoub Aouni and Maha Mastouri,

Application of rep-PCR as a molecular tool for the genetic diversity assessment of Jatropha curcas

February 2016

Jatropha curcas L., a non-edible Euphorbiaceae oil-rich crop cultivated in subtropical/tropical countries, has gained global attention as a promising renewable resource for biodiesel production. Rep-polymerase chain reaction (PCR) was used to investigate the genetic diversity of 15 populations of J. curcas L. Distinct populations of the plant growing wildly in Mauritius were characterised using three molecular...

Author(s): Sillma Rampadarath, Daneshwar Puchooa, Subhasisa Bal and Rajesh Jeewon

High genetic variation of Portunus pelagicus from Makassar Straits revealed by RAPD markers and mitochondrial 16S rRNA sequences

February 2016

The blue swimmer crab, Portunus pelagicus, is presently managed as a single stock. In this study, random amplified polymorphic DNA (RAPD) markers and 16S rRNA sequences were used to understand the genetic diversity and parentage of P. pelagicus from Makassar Strait. A total of 150 samples were collected from the sea, grouped by gender and morphological features. DNA genome was performed at approximately 23130 base pairs...

Author(s): Yushinta Fujaya, Andi Ivo Asphama, Andi Aliah Hidayani, Andi Parenrengi and Andi Tenriulo

DNA-based identification of Lentinula edodes strains with species-specific primers

February 2016

Lentinula edodes is among the five globally cultivated edible mushrooms, which are wood decaying spore bearing Basidiomycetes possessing separate hyphae. Specific identification of this fungus from others in the division Basidiomycota using specific primers enables a fast and accurate detection through polymerase chain reaction (PCR). As a prelude to additional nutritional and sequence characterization research, we have...

Author(s): Pheba L. George, Venkateswara R. Sripathi, Seloame T. Nyaku, Govind C. Sharma and Ramesh V. Kantety

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