African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

AJB Articles

December 2015

Genetic variations and evolutionary relationships among radishes (Raphanus sativus L.) with different flesh colors based on red pigment content, karyotype and simple sequence repeat analysis

To determine the genetic diversity and evolutionary relationships among red radishes, 37 accessions with different flesh colors were analyzed in terms of the red pigment content, karyotypes, and simple sequence repeat markers. Red pigment content of red radish was 3.4 to 28.8% with an average of 15.62%. The karyotype formulas were 14 m (median) + 4 sm (submedian), 16 m + 2 sm, and 18 m for radishes with the same number...

Author(s): Fabo Chen, Hongfang Liu, Qilun Yao, Ping Fang and Fasheng Lv

December 2015

Fertilizer use efficiency by maize (Zea mays) and egusi-melon (Colocynthis citrullus) in various cropping ratios in an ultisol in Edo rainforest area of Nigeria

Three separate field studies were conducted in a rainforest area to determine efficient use of applied fertilizers by maize and egusi-melon in various ratios of mixtures in an ultisol in Nigeria. The experiment was a factorial combination of seven cropping ratios of maize and egusi-melon (MA:EM 1:0, 1:1, 2:1, 3:1, 1:2, and 1:3, respectively) tested under four levels of NPK 20:10:10 (0, 200, 400, and 600 kg/ha)...

Author(s): J. O. Ehigiator, G. O. Iremiren and E. J. Falodun

December 2015

Production of ethanol and polyethanol by yeasts isolated from date (Phoenix dactylifera L.) wastes

The production of dates (Phoenix dactylifera L.) in Algeria generates each year, considerable quantities of waste that weaken the balance of our environment and are very rich in organic matter. Their valuation by biotechnological processes enables the production of high value added materials with low cost. In this regard, the objective of this study focused on the selection of yeasts that can be used to produce ethyl...

Author(s): Rezki Meriem Amina, Aouad Linda and Bekki Abdelkader

December 2015

Blood glucose response of normoglycemic adults fed breakfast porridges made from Zea mays supplemented with Afzelia africana, Detarium microcarpum or Sphenostylis stenocarpa

Effect of high fibre leguminous products in breakfast porridges, made from Afzelia Africana (AA), Detarium microcarpum (DM), Sphenostylis stenocarpa (SS) and Zea mays on blood glucose level of humans was investigated in this study. The food items were processed into flour using African (Nigerian) traditional processing methods. The flours were combined in a ratio of 4:1, Z. mays-A. africana (CAA), Z. mays-D. microcarpum...

Author(s): Uchenna Agatha Onyechi, Vivienne NkirukaIbeanu and Nkechinyere Gift Ugwumba

December 2015

The simulation analysis of contact characteristics of biomimetic flexible surfaces

Based on the foot structure of the climbing biology and multivariate coupling bionic technology, the bionic flexible convex surface was designed and a 3D model was created using the digital modeling software. Finite Element Analysis software was used for contacting analysis to the bionic flexible convex foot structure in the state of dry friction and wet adhesion, and then studied frictional contact performance. The...

Author(s): Sui Xiuhua, He Jing, Zeng Xianwei, Huang Yunqian and Su Xu

December 2015

Application of solar treatment for the disinfection of geophagic clays from markets and mining sites

Most of the microorganisms occurring in geophagic clays are undesirable and may to some extent be harmful to geophagists; it is therefore important to develop a cheap and sustainable technique for the treatment of these clays prior to consumption. In this study, a solar treatment simulated in a chamber has been investigated for its potential to inactivate the microorganisms found in the geophagic clays obtained from...

Author(s): Fosso-Kankeu E., Netshitanini T. L., Abia A. L. K., Ubomba-Jaswa E. and Waanders F. B.

December 2015

Pathogen detection and gut bacteria identification in Apis cerana indica in Thailand

Pathogen infection of honeybees can lead to economic losses in apiculture. The earlier the pathogen contamination can be found the better it will likely be for the treatment of the infected colony and prevention of the spread of the pathogen within and between colonies. A total of 50 colonies of Apis cerana were sampled in Samut-Songkhram (five colonies) and Chumphon (45 colonies) provinces in the central and the south...

Author(s): Pawornrat Nonthapa and Chanpen Chanchao

December 2015

Adaptability and stability of transgenic soybean lines and cultivars in the Brazilian macroregion 3 assessed by using parametric and nonparametric methods

This study aimed to evaluate the adaptability and stability of 20 soybean genotypes, ten of which were lines of Monsoy Ltd breeding program, whereas the other 10 genotypes were commercially used cultivars. The experiments were carried out in the agricultural years of 2005/2006 and 2006/2007, in Brazil, specifically in the soybean microregions 301, 302 and 303 (part of Minas Gerais, Goiás and São Paulo...

Author(s): Rodrigo Marchiori, Osvaldo Toshiyuki Hamawaki, Gabriel Lemes Jorge, Fernanda Mundim, Ana Paula Oliveira Nogueira, Raphael Lemes Hamawaki and Larissa Barbosa de Sousa

December 2015

Application of multivariate analysis to evaluate the biochemical changes in sorghum (Sorghum bicolor L. Moench) after exposure to water stress and silicon applications

The objective of the present work was to verify, through multivariate analysis, the behavior patterns of biochemical compounds in forage sorghum, submitted to different silicon applications and water stress. Experiment with forage sorghum (Sorghum bicolor L. Moench), variety BR 700, was conducted in a greenhouse. The experimental design was completely randomized in a 2×4 factorial arrangement with seven...

Author(s): Luma Castro de Souza, Glauco André dos Santos Nogueira, Risely Ferraz de Almeida, Leane Castro de Souza, Myriam Galvão, Cândido Ferreira de Oliveira Neto, Tamires Borges de Oliveira, Ismael de Jesus Matos Viégas and Ricardo Shigueru Okumura

December 2015

Phyto-nutrient composition and antioxidative potential of ethanolic leaf extract of Sida acuta in wistar albino rats

Phytochemical, micronutrient composition and anti-oxidative potential of ethanolic leaf extract of Sida acuta in albino wistar rats were investigated using standard analytical methods. The result (mg/100 g) for phytochemical composition were 91.46 ± 0.02 tannin, 1500.36 ± 0.36 alkaloid, 530.27 ± 0.03 saponin, 1163.86 ± 0.1 flavonoid, 1454.50 ± 0.85 steriod, 115.29 ± 0.05...

Author(s): Nwankpa P., Chukwuemeka O. G., Uloneme G. C., Etteh C. C., Ugwuezumba P. and Nwosu D.

December 2015

Surveys of virus diseases on pepper (Capsicum spp.) in South-west Nigeria

Surveys to determine the incidence, diversity and distribution of viruses infecting pepper (Capsicum spp.) were conducted in six states (Oyo, Ondo, Osun, Ogun, Ekiti and Lagos) of South-west Nigeria in 2010 and 2011. Leaf samples from symptomatic and asymptomatic plants were collected at random from farmers’ fields and were analyzed for viruses using antigen coated plate enzyme-linked immunosorbent assay...

Author(s): Arogundade Olawale, Balogun Olusegun Samuel, Akinyemi Sunday O. Solomon and P. Lava Kumar

December 2015

Detection of beet necrotic yellow vein virus in Pakistan using bait-plant bioassay, ELISA and RT-PCR

The Northwestern plains of Pakistan are the major sugar beet producing region in the country, providing an important alternative to sugar cane for sugar production when sugar cane is absent in the fields. We surveyed this region for four consecutive years and found that Beet necrotic yellow vein virus (BNYVV) is prevalent in at least five of these districts (Peshawar, Charsadda, Nowshera, Mardan and Swabi). An increase...

Author(s): Muhammad Arif, Waseemullah Khan, Amna Shafi and Kamranullah

December 2015

Minerals and trace elements in domesticated Namibian Ganoderma species

Edible and medicinal mushrooms are regarded as the ideal health foods. They are well appreciated for their exquisite taste and flavor and are consumed both fresh and in processed forms. Nutritive value of mushrooms is attributed to their high content of essential amino acids, vitamins, minerals and low lipid content. Regular consumption of whole medicinal and edible mushrooms could introduce a functional or medicinal...

Author(s): F. N. Mhanda, N. P. Kadhila-Muandingi and I. S. E. Ueitele

December 2015

Impact of arbuscular mycorrhizal fungus, Glomus intraradices, Streptomyces and Pseudomonas spp. strain on finger millet (Eleusine coracana L.) cv Korchara under water deficit condition

In present study, a total of 104 bacteria and 96 actinomycetes were isolated from rhizosphere plant root of finger millet (Eleusine coracana) from Almora district of Uttarakhand, India. Isolates were characterized using microscopic and morphological methods followed by their biochemical test. Based on functional tests, four most promising isolates were identified using 23S rDNA primers as Streptomyces labedae (SB-9),...

Author(s): Ranveer Kamal, Yogendra Singh Gusain, Ishwar Prakash Sharma, Suvigya Sharma and A. K. Sharma

December 2015

Isolation and characterization of efficient cellulolytic fungi from degraded wood and industrial samples

Cellulose is the most abundant biopolymer and renewable natural product in the biosphere. Cellulose degrading fungal strains play an important role in recycling of cellulosic materials. They have immense advantage in various industries to hydrolyze cellulosic substrates for production of various products. This study was thus aimed to isolate and characterize efficient cellulose degrading fungi from their common natural...

Author(s): Abreham Bekele, Tariku Abena, Adane Habteyohannes, Addisalem  Nugissie, Fitala  Gudeta,  Tigist Getie, Musin Kelel and Admas Berhanu

November 2015

Biological activities of species in the genus Tulbaghia: A review

Since time immemorial, plants have been used by several communities to treat a large number of diseases. Numerous studies on the pharmacology of medicinal plants have been done. Medicinal plants constitute a potential source for the production of new medicines and may complement conventional antimicrobials and probably decrease health costs. Phytochemical compounds in plants are known to be biologically active aiding,...

Author(s): S. Takaidza, M. Pillay and F. M. Mtunzi

November 2015

Techno-economic analysis of fuel ethanol production from cassava in Africa: The case of Tanzania

The abundance of low-cost feedstock is of great importance for reinforcing industrialization of bioethanol as a sustainable source of eco-friendly energy. This paper describes improved techniques that increase the root productivity of cassava (Manihot esculenta) and its conversion to bioethanol. A variety of models and analytical approaches were used to choose the best technologies, considering various levels of...

Author(s): Julián A. Quintero, Carlos A. Cardona, Erika Felix, Jonathan Moncada and Juan C. Higuita

November 2015

Stimulatory activity of four green freshwater sponges on aquatic mycotal communities

The influence of the four species of green sponges (Ephydatia muelleri, Heteromeyenia stepanowii, Spongilla fluviatilis, and Spongilla lacustris) on the occurrence of aquatic mycotal species in the water of three four water bodies of different trophy was investigated in this study. Seeds and snake exuviae were used as baits. For the measurement of the primary and extracellular production by symbiotic algae of green...

Author(s): Bazyli Czeczuga, Ewa Czeczuga- Semeniuk, Adrianna Semeniuk- Grell and Janusz Semeniuk

November 2015

Polyphenolic constituents and antioxidant/antiradical activity in different extracts of Alstonia scholaris (Linn.)

Alstonia scholaris (Linn.) leaves extracted in aqueous, dichloromethane (DCM), methanolic and ethanolic solvents were assessed for different polyphenolic constituents endowed with antioxidant/antiradical activity. Total phenolic, flavonoids and tannin contents were significantly (P<0.05) higher in ethanolic extract as compared to other extracts. The antiradical activity and efficiency was significantly (P<0.05)...

Author(s): Pawan K. Verma, Rajinder Raina, Mudasir Sultana, Shahid Prawez and Maninder Singh

November 2015

Sugarcane in vitro culture technology: Opportunities for Kenya’s sugar industry

Sugarcane (Saccharum officinarum L.) is one of the most important crops in Kenya and has wide range of economic importance. The sugar industry contributes up to 15% to the country’s agricultural gross domestic product and an estimated 25% of the population depends on the industry for their livelihood. However, the industry has been facing several challenges including declining yields due to use of poor quality...

Author(s): Richard Wekesa, Justus M. Onguso, Bernard A. Nyende and Leonard S. Wamocho

November 2015

Production of α-amylase from some thermophilic Aspergillus species and optimization of its culture medium and enzyme activity

This paper aimed to evaluate the thermophilic and hyperthermophilic fungi isolated from extreme conditions capable of secreting α-amylase, an enzyme that has a commercial value especially in the production of bread in the food industry and also to ensure their optimization. In this study, thermostable amylase activities of some thermophilic Aspergillus species were evaluated. The suitable medium and microorganisms...

Author(s): Arzu Unal

November 2015

Bacillus sp. R2 α-amylase production optimization: Pasta cooking water as medium of amylase production

α-Amylases (EC 3.2.1.1; 1,4-α-D-glucanglucanohydrolase) are enzymes that are widely studied and used in industries. Their major source is microorganisms. This study aimed to prepare a readily available medium for industrial amylase production. An optimization of amylase production conditions by Bacillus sp. R2 and medium composition were done. Pasta cooking water was selected as basal medium due to its...

Author(s): Slimane Choubane, Omar Khelil and Ben Amar Cheba

November 2015

Validating simple sequence repeat (SSR) markers for introgression of stay-green quantitative trait loci (QTLs) into elite sorghum lines

Considerable effort has been made to improve drought-stress tolerance in sorghum by incorporating the stay-green trait into drought-susceptible elite sorghum varieties. Keeping track of the several genes involved in the expression of this complex trait during the breeding program is an enormous task. In this study, the fidelity of recently identified SSR markers were tested for introgression of stay-green QTLs into...

Author(s): R. Edema and G. L. Amoding

November 2015

In vitro transformation of pearl millet (Pennisetum glaucum (L). R. BR.): Selection of chlorsulfuron-resistant plants and long term expression of the gus gene under the control of the emu promoter

The plasmids pULGU1 and pEmuGN were introduced by biolistics in embryogenic cell suspensions of pearl millet, Pennisetum glaucum. The plasmid pULGU1 contained the mutant acetolactate synthase (ALS) gene of Arabidopsis thaliana, responsible for resistance to the chlorsulfuron herbicide. The plasmid pEmuGN carried the reporter β-glucuronidase (GUS) gene of Escherichia coli. Two months after bombardment of the cells,...

Author(s): Kouakou Tiécoura, Abou Bakari Kouassi, N’nan-Alla Oulo, Séry Gonedelé Bi, Monique Dinant and Lucien Ledou

November 2015

Influence of enzymes and ascorbic acid on dough rheology and wheat bread quality

The combined action of ascorbic acid and two commercial enzymatic complexes containing amylase and xylanase/amylase was analyzed to determine their effects on dough rheology and bread quality. Seven bread formulations containing different concentrations of these improvers were used in the analysis. The rheological properties of each dough formulation were determined by moisture, gluten and farinograph tests. The breads...

Author(s): César Milton Baratto, Natalia Branco Becker, Jane Mary Lafayette Neves Gelinski and Sydnei Mitidieri Silveira

November 2015

Spectral analysis and anti-bacterial activity of methanolic fruit extract of Citrullus colocynthis using gas chromatography-mass spectrometry

Phytochemicals are chemical compounds often referred to as secondary metabolites. Thirty three bioactive phytochemical compounds were identified in the methanolic extract of Citrullus colocynthis. The identification of phytochemical compounds is based on the peak area, retention time molecular weight and molecular formula. Gas chromatography-mass spectrometry (GC-MS) analysis of C. colocynthis revealed the existence of...

Author(s): Salah Ali Idan, Ali Hussein Al-Marzoqi and Imad Hadi Hameed

November 2015

Investigations of antioxidant and antibacterial activity of leaf extracts of Azadirachta indica

Active ingredients of medicinal plants have been used to cure several human diseases. Azadirachta indica is one of the most versatile medicinal plants having a wide spectrum of biological activity due to the presence of large number of bioactive compounds. The present study was conducted to evaluate antimicrobial and antioxidant efficiency and phytochemical screening of A. indica leaf extract using methanol as a...

Author(s): Bharat Pokhrel, Sagar Rijal, Sulav Raut and Ankit Pandeya

November 2015

In vitro antifungal activity of Dorstenia mannii leaf extracts (Moraceae)

The leaves of Dorstenia mannii are used in traditional medicine in Cameroon and other African countries for the treatment of infectious diseases like malaria, skin rashes and stomach disorders. To substantiate this folkloric claim, the crude methanol extract and fractions from the leaves of D. mannii were investigated for their antifungal activity. The crude methanol extract was prepared from powdered dried leaves of...

Author(s): Kechia Frederick Agem, Gerald Ngo Teke, Ngameni Bathelemy, Fokunang Charles, Dzoyem Jean-Paul and Kamga Henri Lucien

November 2015

Selection of okra parents based on performance and genetic divergence

A total of 200 okra accessions with wide variability and a potential for genetic improvement were stored in the Vegetables Germplasm Bank of the Federal University of Viçosa (UFV-BGH) in Viçosa, Minas Gerais State, Brazil. The objective of this work was to select parents by genetic divergence and behavior per se in 70 okra accessions from the BGH-UFV by quantitative and qualitative descriptors of economic...

Author(s): André Pugnal Mattedi, Bruno Soares Laurindo, Derly José Henriques da Silva, Carlos Nick Gomes, Leonardo Lopes Bhering and Moacil Alves de Souza

November 2015

Evaluation of genetic divergence and phylogenetic relationship using sequence-tagged microsatellite (STMS) sequences in Chickpea (Cicer arietinum L.) genotypes

The current investigation was carried out to analyse the genetic diversity estimates between 125 chickpea genotypes using sequence-tagged microsatellite (STMS) markers. Thirty one STMS primers generated a total of 153 loci (an average of 4.94 loci per primer) out of which 129 loci were found to be polymorphic and 24 loci were monomorphic. The value of PIC varied from 0.128 to 0.783 while the resolving power varied from...

Author(s): Himanshu Aggarwal, Alka Rao, Anil Kumar, Jasbir Singh, Jogender Singh Rana, Pradeep Kumar Naik and Vinod Chhokar

November 2015

Genetic diversity of bitter and sweet African bush mango trees (Irvingia spp., Irvingiaceae) in West and Central Africa

Economically important food tree species in sub-Saharan Africa should be domesticated to enhance their production within agro forestry systems. The African bush mango trees (Irvingia species) are highly preserved and integrated in agro forestry systems in tropical Africa. However, the taxonomic debate related to the species or varietal status of the bitter and sweet fruited African bush mango trees hinders their...

Author(s): Vihotogbé, R., van den Berg, R. G., Missihoun, A. A., Sinsin, B., and Sosef, M. S. M.,

November 2015

Glycerine associated molecules with herbicide for controlling Adenocalymma peregrinum in cultivated pastures

The weed Adenocalymma peregrinum that is popularly known in Brazil as “ciganinha”, belongs to the family Bignoniaceae. The only way to control this plant species in crop fields is by the application of herbicides on the stump or directly on the stem. The present study aimed to analyze the effect of glycerine in controlling A. peregrinum (MIERS) L.G.Lohmann when applied on the stem. The glycerin used as a...

Author(s): Rejanne Lima Arruda, Melquezedeque do Vale Nunes, Paulo Roberto da Silva , Fernando Ferreira Leão (In memoria), Renato de Almeida Sarmento , Thomas Vieira Nunes and Eduardo Andrea Lemus Erasmo

November 2015

Response of maize (Zea mays L.) to combined application of organic and inorganic (soil and foliar applied) fertilizers

A field trial was carried out in the 2013 cropping season at the Teaching and Research Farm of Kwara State University, Malete, Nigeria (08° 42” 48.5N and 004° 26 17.9” E ) to assess the response of early maturing maize variety (TZEE-Y) when using organic poultry manure (pm) alone or in combination with inorganic (NPK) and foliar fertilizer (ff) (boost xtra). The treatments were: pm 5.0 t ha-1 + ff,...

Author(s): A. I. Afe, S. Atanda, M. O. Aduloju, S. K. Ogundare and A. A. Talabi

November 2015

Susceptibility of Algerian pepper cultivars (Capsicum annuum L) to Phytophthora capsici strains from different geographic areas

Ten pepper cultivars (Capsicum annuum L), that is, Doux D’Alger, Sonar, Esterel, Doux, Marconi, Magister, Belconi, Italico II, Lipari, Arabal, and Doux d’Espagne commercially grown in Algeria were inoculated with six isolates of Phytophthora capsici Leon, and susceptibility of pepper was assessed by evaluation of disease severity in different plant organs. The cultivar Italico II was found to be more...

Author(s): Benabdelkader Messaouda, Guechi Abdelhadi and Mézaache-Aichour Samia

November 2015

Drying of enzyme immobilized on eco-friendly supports

Endophytic fungus Cercospora kikuchii lipase was immobilized on agroindustrial by-products and dried by oven, freeze and spray drying. Spray drying showed the best performance regarding the drying technologies evaluated. Microcrystalline cellulose and rice husk showed the best result since they retained almost 100% of lipase activity after drying. Immobilized derivatives obtained had decreased enzyme activity (»...

Author(s): Costa-Silva T. A., Souza C. R. F., Said S. and Oliveira W. P.

November 2015

Purification and characterization of phenoloxidase from immunized haemolymph of Schistocerca gregaria

Phenoloxidase (PO) is a key factor in insect immunity. On invasion of microorganisms and pathogens, prophenoloxidase (PPO) changes to its active form, PO. The present study has been conducted to purify and characterize the PO from the haemolymph of desert locust, Schistocerca gregaria (Forskal) following activation of immune system by invasion of bacteria, Bacillus thuringiensis kurstaki (Bt). PO is purified by a...

Author(s): Mahmoud, D. M., Salem, D. A. M., Mo&#;men, S. A., Barakat, E. M. S. and Salama, M. S.

October 2015

Genetic characterization of angular leaf spot resistance in selected common bean landraces from Tanzania

Angular leaf spot disease (ALS) caused by Pseudocercospora griseola is one of the most important bean diseases in Tanzania. The bean landraces Beti-10, Nanka, Nanavala and Nkanamna used in this study have been identified as resistant to ALS but the nature of inheritance and mechanisms of resistance against ALS in those potential sources has not been elucidated. This information is crucial and a necessary first step for...

Author(s): George Muhamba Tryphone, Luseko Amos Chilagane, Susan Nchimbi-Msolla and Paul Mbogo Kusolwa

October 2015

Laboratory study on influence of plant growth promoting rhizobacteria (PGPR) on growth response and tolerance of Zea mays to petroleum hydrocarbon

The influence of rhizobacteria on the growth and tolerance of Zea mays (maize) in a petroleum hydrocarbon (crude oil) impacted medium was investigated. This study evaluated the effect of inoculating maize seeds with plant growth promoting rhizobacterial strains in a crude oil impacted medium. The rhizobacterial strains used in this study were tested for plant growth promoting traits [indole-3-acetic acid production,...

Author(s): Chinyere Augusta Ajuzieogu, Abiye Anthony Ibiene and Herbert Okechukwu Stanley

October 2015

Milk quality in high production systems during dry and rainy seasons according to normative instruction N° 62

This study aimed to evaluate the milk quality of crossbred cows from five production systems according to the quality parameters required by Normative Instruction No. 62 (NI 62). Five different production systems were used, with different environmental and sanitary conditions, and with animals from different breeds in two seasons: dry and rainy. Three individual milk samples were collected from animals in the five...

Author(s): Cristiane Isabô Giovannini, Priscila Alonso dos Santos, Thiago Soares de Carvalho, Jakeline Fernandes Cabral and Thamara Evangelista Silva

October 2015

Bioguided investigation of the antimalarial activities of Trema orientalis (L.) Blume leaves

Malaria remains a major public health concern which affects millions of people, particularly in Sub-Saharan Africa. The need for the development of alternate treatment means has become critical because of the emergence of resistance to nearly all antimalarial drugs (Kim and Schneider, 2013). Trema orientalis (L.) Blume (Ulmaceae) is used locally for the treatment of malaria. This study was designed to determine the...

Author(s): Samuel Babatunde, , Oluyemi Wande Michael and Abiodun Oyindamola

October 2015

Antibacterial activity of secondary metabolites isolated from Alternaria alternata

The aims of this study were the analysis of the secondary metabolites and evaluation of the antibacterial and antifungal activity of Alternaria alternata. Twenty six bioactive compounds were identified in methanolic extract of Alternaria alternata. The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC-MS analysis of A. alternata revealed...

Author(s): Sabreen A. Kamal, Lena Fadhil Hamza and Imad Hadi Hameed

October 2015

Drug resistant Salmonella in broiler chicken sold at local market in Bangladesh and its public health significance

Salmonella is a globally widespread food-borne pathogen having major impact on public health. Salmonellosis is an infection with bacteria called Salmonella. Most persons infected with Salmonella develop diarrhea, fever, and abdominal cramps 12 to 72 h after infection. This study was designed to isolate and identify Salmonella spp. from cloacal swabs of apparently healthy broiler chickens in Bangladesh. Salmonella was...

Author(s): Abanish Aditya

October 2015

Effect of reproductive status on body condition score, progesterone concentration and trace minerals in sheep and goats reared in South Sinai, Egypt

This study aimed at evaluating the effect of reproductive stage on nutritional status, and hormonal and trace mineral levels in sheep and goats reared in harsh arid conditions in South Sinai, Egypt. Egyptian local breeds of sheep (n=74) and goats (n=58) raised in South Sinai were examined by means of transabdominal ultrasonography to verify single vs. multiple feti pregnancy or non-pregnant status. Serum samples were...

Author(s): Mahmoud Fathy Nawito, Karima Ghonaimy M. Mahmoud, Mohamed Mahmoud M. Kandiel, Yousef F. Ahmed and Ahmed Sayed A. Sosa

October 2015

Comparison of anther and microspore culture in androgenic embryogenesis and regeneration of broccoli (Brassica oleracea L. var. italica P.)

The aim of this study was to compare the efficiency of broccoli anther and microspore culture methods for doubled haploid (DH) lines production. We evaluated the main influencing factors and optimized the culture methods to improve embryo induction and plant regeneration for efficient doubled haploid production in broccoli breeding. Six broccoli hybrids were used in this study. Our results show that generally, the...

Author(s): Ying Qin, Yunyu Huang, Seppo Pulli and Yang-Dong Guo

October 2015

Suppressive effects of a polymer sodium silicate solution on powdery mildew and root rot diseases of miniature rose

Sodium silicate was dissolved in water in either a monomer form or polymer form; the effects of both forms of sodium silicate aqueous solution on rose powdery mildew and root rot diseases of miniature rose were examined. Both forms of sodium silicate aqueous solution were applied to the roots of the miniature rose. Potassium silicate aqueous solution was used as a control and was compared to the effect of sodium...

Author(s): Mohsen Mohamed Elsharkawy, Tatsuya Hase, Masafumi Shimizu and Mitsuro Hyakumachi

October 2015

Toxic effects of Ricinus communis non-protein trypsin inhibitor on Spodoptera frugiperda (J. E. Smith) (Lepidoptera:Noctuidae)

In the study reported herein, we aimed to isolate a trypsin inhibitor from Ricinus communis leaves through chromatographic and spectrometric techniques and evaluate its toxic effects on the development of Spodoptera frugiperda larvae. Plant extracts were submitted to fractionation in adsorption column. The fraction 10, which showed the highest inhibitory activity, were incorporated into an artificial diet at the...

Author(s): Gislaine A. Carvalho, Custódio D. dos Santos, Dejane S. Alves, Geraldo A. Carvalho, Maria das G. Cardoso and Marcelo M. de Haro

October 2015

The efficacy of palm oil sludge in reducing ruminal methanogenesis using rumen simulation technique

The effect of including palm oil sludge in cattle diet (silage) was conducted in vitro in order to ascertain the suppressing effect on ruminal methanogenesis. The silage used for the study was Panicum maximum, Centrocema pubscens, Bracharia decumbens and Andropogon gayanus. The silage were cut into an average size of 3 to 5 cm and ensiled for 21 days. Four treatment diets were formulated with the silage by including...

Author(s): L. C. Ugwuowo, A. G. Ezekwe, A. O. Ani, S. I. Eze, C. N. Anyanwu and A. Ofomatah  

October 2015

Heterogeneous expression and functional evaluation of in silico characterized recombinant OmpC of Salmonella Typhimurium as a functional poultry vaccine to eradicate zoonotic transmission

Salmonellosis is one of the major global health concerns leading to millions of deaths annually. The present vaccines not being up to the mark necessitate the need for the development of new generation vaccines. Outer membrane proteins (Omps) of several Gram negative bacteria have been investigated and found to be immunogenic and protective. The present study explores the potential of a major porin protein (OmpC) of...

Author(s): Richa Jha, Anil Kumar, Anjani Saxena, Mamta Pandey, Rajesh Kumar and Mumtesh Kumar Saxena

October 2015

Use of simple sequence repeat (SSR) markers for screening blue disease resistance in cotton germplasm exchange

Blue disease of cotton is an economically important disease of the crop first described from the Central African Republic and spread to other countries. Brazil and other South American countries record crop losses of up to 80% from infection but no cases of the disease have been reported in Tanzania. Resistance to the disease has been found in African germplasm and transferred to crop cultivars worldwide. Molecular...

Author(s): Christopher Faustine, Lucia Vieira Hoffmann, Flora Ismail Tibazarwa and Everina Lukonge

October 2015

Antibiosis in Ascia monuste orseis Godart (Lepidoptera: Pieridae) caused by kale genotypes

Ascia monuste orseis (Lepidoptera: Pieridae) is one of the main insect pests of kale. The study was done to identify kale varieties resistant to A. monuste orseis by the antibiosis resistance mechanism. Kale genotypes (26) were evaluated in experiments performed at the Laboratory of Agricultural Entomology of Goiano Federal Institute - Campus Urutaí. A completely randomized experimental design with 50 replicates...

Author(s): Luciano Nogueira, Lígia Alves de Paiva, Andre Cirillo de Sousa Almeida, Zulene Antonio  Ribeiro Arlindo Leal Boica Junior and Flavio Goncalves de Jesus

Page 19 of 245, showing 50 records out of 12240 total, starting on record 901, ending on 950