African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

AJB Articles

October 2015

Estimation of genetic diversity between three Saudi sheep breeds using DNA markers

The genetic variation of Najdi, Harri and Awassi breeds of Saudi sheep prevailing in Raniah province of Makka district were assessed and compared to Sudanese Desert sheep using random amplified polymorphic DNA polymerase cahin reaction (RAPD-PCR) technique. Five primers successfully amplified distinguishable 40 bands with an average of 96% polymorphism revealing that Saudi sheep breeds possess the needed genetic...

Author(s): Ahmed Abdel Gadir Adam, Nada Babiker Hamza, Manal Abdel Wahid Salim and Khaleil Salah Khalil

October 2015

Determination of lactic acid bacteria in Kaşar cheese and identification by Fourier transform infrared (FTIR) spectroscopy

Lactic acid bacteria (LAB) arise in Kaşar cheese, an artisanal pasta filata cheese produced in Turkey from raw milk without starter addition or pasteurized milk with starter culture. In this study, 13 samples of Kaşar cheese that were produced from raw milk were used as reference materials. LAB were characterized by using phenotypic, API and Fourier transform infrared (FTIR) spectroscopy methods. One hundred and...

Author(s): İlkay Turhan and Zübeyde Öner

October 2015

Profile, antioxidant potential, and applicability of phenolic compounds extracted from Spirulina platensis

This work aimed at an investigation of the profile of free phenolic compounds (PC) of Spirulina platensis and assesses their antioxidant potential, applying them as a natural conservative in minimally processed apples. The phenolic extract showed 396 µg g-1 gallic acid, 347  µg g-1 of caffeic acid, 54 µg g-1 salicylic acid and 3.5 µg g-1 trans-1-cinmamic a total of 608 µg PC g-1 of S....

Author(s): Taiana Denardi de Souza, Luciana Prietto, Michele Moraes de Souza and Eliana Badiale Furlong

October 2015

Analysis of bioactive chemical components of two medicinal plants (Coriandrum sativum and Melia azedarach) leaves using gas chromatography-mass spectrometry (GC-MS)

The main objective of this study was to determine the phytochemical composition in the leaves of Coriandrum sativum, using methanolic extraction and report the main functional components by using IR technique. The phytochemical compounds in the extract were then screened by GC-MS method. Seven bioactive phytochemical compounds were identified in the methanolic extract of C. sativum: 1,6-octadien-3-ol, 3,7-dimethyl,...

Author(s): Ali Hussein Al-Marzoqi, Imad Hadi Hameed and Salah Ali Idan

October 2015

In vitro regeneration of Calophyllum brasiliense Cambess: A valuable medicinal tree

Calophyllum brasiliense (Calophyllaceae) is a medicinal tree known mainly for producing calanolides, secondary metabolites against HIV-1 reverse transcriptase. This wild plant is listed as threatened and despite its outstanding medicinal value, no studies have been conducted on its propagation or preservation. This study standardized a procedure for the micropropagation of C. brasiliense with nodal segments from in...

Author(s): A. Maldonado-Magana, A. Bernabe-Antonio, E. Salcedo-Perez and F. Cruz-Sosa

October 2015

Gene mining a marama bean expressed sequence tags (ESTs) database: Embryonic seed development genes and microsatellite marker identification

Tylosema esculentum (marama bean) is one of the underutilized legumes that have potential to provide protein and fatty acids to ensure food security in dry parts of Southern Africa. In order to establish rapid domestication programs for the plant, it is important to explore the plant’s genome and identify functional genes molecular markers like microsatellites in order to develop molecular tools. With the advent...

Author(s): Emilia N. Sheehama and Percy M. Chimwamurombe

October 2015

Multiploidy occurrence in tomato calli from anther culture

Anther culture has long been used for the production of fully homozygous lines in order to produce, mainly, doubled haploid plants, which are of great interest in plant breeding. For tomato, a recalcitrant species for androgenesis production protocols have not been standardized. It is known that the genotype, anther size, the developmental stage of the microspore, and the medium composition are some factors that can...

Author(s): Sirlei Aparecida Julião, Carlos Roberto Carvalho, Thaís Cristina Ribeiro da Silva and Andréa Dias Koehler

October 2015

Extraction and physicochemical characterization of a potential multifunctional pharma-excipient from crab shell wastes

An efficient and beneficial waste disposal mechanism is highly desired. This study was carried out to extract and characterize a potential multifunctional pharmaceutical excipient from crab shell wastes. Shells of Pachygrapsus mamoratus were obtained from Oron, a coastal town in Akwa Ibom State of Nigeria. Chitin was extracted from the powdered shell by deproteination and demineralization; and chitosan was derived by...

Author(s): Emmanuel O. Olorunsola, Olubunmi J. Olayemi, Stephen O. Majekodunmi and Unyime B. Etukudo

September 2015

A protocol for Agrobacterium-mediated transformation of Kalanchoë blossfeldiana with a flavonoid 3',5' hydroxylase (F3'5'H) gene

In the present investigation, explants from Kalanchoë blossfeldiana were used for gene transformation. The young leaves were inoculated with Agrobacterium tumefaciens LBA4404 strain with a binary vector plasmid pArtblue containing F3'5'H gene under control of CaMV35S promoter and nptII selectable marker gene. After inoculation, the explants were transferred to the co-cultivation medium. They were then...

Author(s): Phopgao Buddharak, Ruttaporn Chundet and Warut U-kong

September 2015

Effect of arbuscular mycorrhizal fungal inoculation on growth, and nutrient uptake of the two grass species, Leptochloa fusca (L.) Stapf and Sporobolus robustus Kunth, under greenhouse conditions

The aim of our work was to assess the effect of inoculation with three arbuscular mycorrhizal fungi (AMF) (Rhizoglomus aggregatum (N.C. Schenck and G.S. Sm.) Sieverd., G.A. Silva and Oeh., Funneliformis mosseae (T.H. Nicolson and Gerd.) C. Walker and A. Schüssler. and Rhizoglomus intraradices (N.C. Schenck and G.S. Sm.) Sieverd., G.A. Silva and Oehl.), and a mixed inoculum of these AMF on root colonization, biomass...

Author(s): Fatoumata Fall,, Diégane Diouf,, Dioumacor Fall,, Ibrahima Ndoye,, Cheikh Ndiaye, Aboubacry Kane, and Amadou Mustapha Bâ,

September 2015

Cooking and drying processes optimization of Pentadesma butyracea kernels during butter production

The cooking and the drying of the Pentadesma butyracea kernels are the main steps in the traditional method of extracting P. butyracea butter. In this work, the Response Surface Method with Central composite design was used to optimize the cooking and drying of Pentadesma kernels during P. butyracea butter production. The cooking and drying times, as well as the drying temperature of Pentadesma kernels are considered as...

Author(s): Eric Badoussi, Paulin Azokpota, Yann E. Madodé, Fidèle P. Tchobo, Balbine Fagla Amoussou, Adéchola P. P. Kayodé, Mohamed M. Soumanou and D. Joseph Hounhouigan

September 2015

Use of pectin in the storage of mangaba fruits (Hancorniaspeciosa Gomes) associated with refrigeration

The objective of this study was to evaluate the storage potential of mangaba fruits coated with pectin biofilm stored in biochemical oxygen demand (BOD) (22 ± 1°C) under modified atmosphere. Scanning electron microscopy (SEM) was used to analyze the physical and chemical changes, vitamin C content, soluble solid, titratable acidity and turgor pressure, and colour and physical structure of the fruits during...

Author(s): Plácido, G. R., Silva, R. M., Cagnin, C.,Silva, M. A. P., Soares, J. C. and Caliari, M.

September 2015

Fermentable sugars and microbial inhibitors formation from two-stage pretreatment of corn stalk with variation in particle size and severity factor

Microbial inhibitors including weak acids, furan derivatives and phenolic compounds are key problems of cellulosic bio-fuels production by fermentation. Most of these inhibitors are sugars and lignin degradation compounds, which are almost unavoidable during pretreatment processes. While, most of the one stage pretreatment has been conducted at high severity factors of 3.5 or more to get high sugar yield, with increase...

Author(s): Nawa Raj Baral, Jianzheng Li and Ajay Kumar Jha

September 2015

Genetic variability of indigenous cowpea genotypes as determined using inter-simple sequence repeats markers

Cowpea [Vigna unguiculata (L.) Walp.] is cultivated widely by small farmers in the semiarid region of Northeastern Brazil for subsistence purposes, especially to complement the family income. However, owing to the limited availability of water in this region, there is an urgent need for novel highly productive drought-tolerant cultivars. The aim of the present study was to establish the genetic variability of 14 cowpea...

Author(s): R. F. M. Mendes, F. B. Britto, F. R. Freire Filho and P. S. C. Lima

September 2015

Preliminary characterization of residual biomass from Hibiscus sabdariffa Calyces

Hibiscus sabdariffa calyces are mainly used for different agro-food and beverages applications. The residual biomass generated contains various useful substances that were extracted and characterized. It contained 23% (w/w) soluble pectic material, a food additive, extracted with hot acidified water (80°C, pH = 1.5) and precipitated with ethanol. The molecular weight (28.5 and 109.7 kDa), the degree of methylation...

Author(s): Cheikh Beye, , Mario Aguedo, Serge Hiligsmann, Lat Souk Tounkara, Aurore Richel and Philippe Thonart

September 2015

A study of the optimal conditions for glucoamylases obtained from Aspergillus niger using amylopectin from cassava starch as carbon source

A fourteen day pilot study carried out showed that high glucoamylase activities were obtained on the 4 and 11th day of fermentation and the enzymes were harvested on the respective days giving the codes GluAgCSV4 and GluAgCSV11. The optimal pH and optimal temperatures for enzyme activities GluAgCSV4 and GluAgCSV11 were in a range of 6 to 7 and 50 to 55, using cassava, guinea corn and tiger nut starch as substrates,...

Author(s): Ezugwu, A. L., Eze, S. O. O. and Chilaka, F. C.

September 2015

Anatomical and histochemical analysis of vegetative organs of Vernonia ferruginea Less. (Asteraceae)

Vernonia ferruginea Less. is a perennial shrub species, present in several regions of Brazil, especially in the savanna. It is popularly used as a phytotherapic. This fact justifies the need to anatomically characterize the plant for its accurate identification and to conduct histochemical studies with the aim of identifying the chemical nature of its cellular constituents. The species-specific data will contribute...

Author(s): Oliveira, R. C. de, Vasconcelos Filho, S. C., Bastos, A. V. S., Vasconcelos, J. M. and Rodrigues, A. A.

September 2015

Chemical composition and mutagenic assessment of petrochemical effluents on onion (Allium cepa) root tip mitosis

The continous production and release of chemicals into the environment have led to the need to assess their chemical composition and genotoxic effects on cell reproduction. Two petrochemicals, air liquid and polyester resin effluents were assessed. The common onion of the purple variety was used as the test organism. The results of the chemical analysis of the wastewaters showed high concentration of some potentially...

Author(s): Abu, Ngozi E., Asomba, C. O. and Ubani, C.

September 2015

Management of Fusarium Wilt using mycolytic enzymes produced by Trichoderma harzianum (Th. Azad)

The main aim of this study was to isolate the best chitinase and glucanase enzyme producing Trichoderma strain to manage the Fusarium wilt disease of Cicer aritenum under in vitro conditions. We also studied the effect of Trichoderma strains on the growth and development of C. aritenum plants. Seven strains of Trichoderma were screened against the Fusarium pathogen to isolate the best biocontrol agent causing maximum...

Author(s): Mohammad Shahid, Mukesh Srivastava, Sonika Pandey, Vipul Kumar, Anuradha Singh, Shubha Trivedi, Y. K. Srivastava and Shiv Ram

September 2015

β-Lactam and chloramphenicol-resistant enterobacteria in hospital surfaces

The following study aimed to research the Enterobacteriaceae present on the material surfaces of a hospital environment in a Community Health Unit in Ceara-Brazil. Data was collected in 10 different rooms and facilities by rubbing sterile swabs in an enclosed area of 10 cm² for a minute. Bacterial growth was observed in all surveyed areas. However, Enterobacteriaceae were only found in surfaces from the kitchen and...

Author(s): Jean Linhares de Lima, Sara Edwirgens Costa Benicio Vasconcelos, Theodora Thays Arruda Cavalcante, and Renata Albuquerque Costa,

September 2015

A direct and sensitive method for screening fructooligosaccharides-digesting microorganisms useful in food and health science

Short-chain fructooligosaccharides (FOS) stimulate selectively the growth and activity of microorganisms in the colon providing positive health effects and well-being in humans and animals. The lack of accurate isolation methods, however, hampers the possibility of getting new potential fructo-oligosaccharides (FOS)-fermenting yeast or bacterial strains. A valuable screening procedure to visually detect bacterial and...

Author(s): Trujillo L. E., Granda S., Ordoñez M. I., Ramos T. I. and Avalos R. 

September 2015

Nanoparticle (MPG)-mediated delivery of small RNAs into human mesenchymal stem cells

The cellular membrane constitutes an effective barrier that protects the complex, yet highly ordered, intracellular compartment of the cell. Passage of molecules across this barrier is highly regulated and highly restricted. Cell penetrating peptides (CPPs) are a class of small cationic peptides that are able to defy the rules of membrane passage and gain access to the intracellular environment. MPG is one member of...

Author(s): Nnaemeka Darlington Ndodo,

September 2015

Development of stable marker-free nuclear transformation strategy in the green microalga Chlorella vulgaris

Although microalgae have valuable resources with great necessity in many biotechnological applications, no tools have been developed yet for a stable genetic transformation without antibiotic marker genes in these organisms. Chlorella is one of the most useful genus for biotechnological applications. The transfer of foreign DNA (vector or linear DNA cassette) into Chlorella by electroporation has very low stability and...

Author(s): Ahmed E. Gomma#, Sung-Kwon Lee#, Sang Mi Sun, Seung Hwan Yang, and Gyuhwa Chung

September 2015

Screening of the kernels of Pentadesma butyracea from various growing sites of Benin and evaluation of their antioxidant pigments content

Pentadesma butyracea Sabine (Clusiaceae) is a ligneous forest species of multipurpose uses. It is widely distributed in Africa from Guinea-Bissau to the West of the Democratic Republic of Congo. This study screened the kernel of P. butyracea on the basis of their physico-chemical properties. Six types of kernels were distinguished. The plant producing type 1 kernels (with medium length, low width and high thickness) and...

Author(s): Bernolde P. Ayegnon, Adéchola P. P. Kayodé, Glawdys Gnanvi, Yann madodé, Fagla-Amoussou Balbine, Paulin Azokpota, Mohamed M. Soumanou and Joseph D. Hounhouigan

September 2015

Essential oil composition of different fractions of Piper guineense Schumach. et Thonn from Cameroon using gas chromatography-mass spectrometry and their insecticidal effect on Sitophilus oryzae (L.)

Essential oil fractions from dried seed powder of Piper guineense were analyzed by gas chromatography-mass spectrometry (GC-MS) and evaluated for their insecticidal effects on Sitophilus oryzae L. The GC-MS analysis showed quantitative and qualitative differences between the oil fractions. Chromatographic results revealed chemical constituents like eugenol, piperanol, pinene, carene, copaene with insecticidal...

Author(s): Esther Obi Oben⊃, Robyn McConchie⊃, Kim Phan-Thien⊃ and Nelson Neba Ntonifor⊃

September 2015

Heritability of polyphenols, anthocyanins and antioxidant capacity of Cameroonian cocoa (Theobroma cacao L.) beans

This study investigates the heritability of polyphenolic, anthocyanin and antioxidant capacity of beans derived from four cocoa clones and their offsprings. These compounds were analyzed at 280 nm (polyphenols) and 520 nm (anthocyanins) by reversed-phase high-performance liquid chromatography (RP-HPLC) method using a photodiode array detector (PDA). The antioxidant capacity of methanolic extracts prepared from cocoa...

Author(s): Pierre Effa Onomo, Nicolas Niemenak, Pierre François Djocgoue, Martine Louise Ondobo and Denis Omokolo Ndoumou

September 2015

Inflorescence bulbils of tiger lily in vivo and bulbils culture in vitro

The present study includes two experiments; the physiological and the biotechnological experiments. The physiological experiment was designed to investigate the most interesting rare natural phenomenon of the development of vegetative reproductive organs (bulbils) in the inflorescence of tiger lily. In the other hand, the biotechnological experiment was carried out to evaluate the regeneration potential of bulbils...

Author(s): Hassan M. Asker

September 2015

Effects of different culture conditions (photoautotrophic, photomixotrophic) and the auxin indole-butyric acid on the in vitro acclimatization of papaya (Carica papaya L. var. Red Maradol) plants using zeolite as support

Plant regeneration of papaya via organogenesis and somatic embryogenesis has been successful; however, the biggest problem of in vitro culture of this species is the acclimatization of regenerated plants, where over 70% of the plants are lost before being planted in the field. Decreasing the relative humidity inside the culture vessel and thus increasing the ventilation, appears to have a greater effect on the...

Author(s): Laisyn Posada Pérez, Yenny Padrón Montesinos, Justo González Olmedo, Romelio Rodríguez Sánchez, Osvaldo Norman Montenegro, Raul Barbón Rodriguez, Ortelio Hurtado Ribalta, Rene Carlos Rodriguez Escriba, Dion Daniels and Rafael Gómez-Kosky

September 2015

The effects of exotic weed Flaveria bidentis with different invasion stages on soil bacterial community structures

A new exotic weed, Flaveria bidentis, is spreading in central China where it forms dense monospecific patches modifying the structure of different native ecosystems and threatening native aboveground biodiversity. However, little is known about the consequences of such an invasion for soil bacterial community, especially its effect pattern at different invasion stages. In this study, soil samples were taken in native...

Author(s): Chaohe Huangfu, Huiyan Li, Xinwei Chen, Hongmei Liu and Dianlin Yang

September 2015

Extraction and characterization of Retama monosperma fibers

The aims of this study were to determine the good conditions for fibers extraction from Retama monosperma young stems and their mechanical, physical and chemical characteristics. The fibers were extracted using a range of NaOH concentration from 1 to 16% in a period of treatment of 1 to 24 h, coupled with a physical treatment. For the evaluation of physico-mechanical characteristics, 200 samples were performed in the...

Author(s): AIZI Djamel Eddine and KAID HARCHE Meriem

August 2015

Analysis of the genetic diversity of selected East African sweet potato (Ipomea batatas [L.] Lam.) accessions using microsatellite markers

Sweet potato (Ipomea batatas [L.] Lam.) is an economically important crop in East Africa chiefly grown by small holder farmers. Sharing of vines for planting is a very common occurrence among these farmers and eventually varieties are given local names, making it hard to trace the original pedigree. It is therefore important to characterise the sweet potato germplasm for purposes of breeding and germplasm conservation....

Author(s): Ochieng, L. A., Githiri, S. M., Nyende, B. A., Murungi, L. K., Kimani, N. C., Macharia, G. K. and Karanja, L.

August 2015

Impact of the application of humic acid and sodium nitroprusside on nickel toxicity: Analysis of relative gene expression

Nickel (Ni) is an essential micronutrient for plants but in high concentrations may turn toxic. This paper discusses the potential role of humic acid (HA) and sodium nitroprusside in modulating or preventing oxidative stress in rice plants. Three genes [superoxide dismutase (SOD) glutathione reductase (GR) and ascorbate peroxidase (APx) were selected for an expression study using a real time PCR technique. Three...

Author(s): Sandra Perez Alvarez, Daniel Cabezas Montero, Norma Elena Leyva Lopez, Jesús Mendez Lozano and Esteban Sanchez Chavez

August 2015

Screening of successive extracts of Amorphophallus konjac for antibacterial activity

Amorphophallus is an Aroid family member, native to Asia. Amorphophallus konjac K. Koch ex N.E.Br. is also known as snake plant due to snake like outlines on its stem. In Mount Abu the plant is grown in wild and known for its toxic principles. In Traditional Chinese System of Medicine (TCM), it was mentioned that gel prepared from flour has been used in detoxification, tumor suppression, phlegm liquefaction and skin...

Author(s): Adnan Khokar and Ekta Menghani

August 2015

Lipid lowering and anti-atherosclerotic properties of Tinospora crispa aqueous extract on high-cholesterol diet-induced hyperlipidemic rabbits

This study was aimed to investigate the hypocholestrolemic and anti-atherosclerotic properties of Tinospora crispa aqueous extract (TCAE) on rabbits for 10 weeks. The hyperlipidemic rabbits were induced and the rabbit were given different concentration of TCAE (200, 450 and 600 mg/kg). Results from lipid analysis show that the level of total cholesterol (TC), triglyceride (TG) and LDL-C on the hyperlipidemic rabbits...

Author(s): Zamree, M. S., Ihsan Safwan, K., Khairul Kamilah A. K., Mohd Kamal N. H., Rasadah, M. A., Mohd Shahidan M. A., Daryl, J. A. and Zulkhairi, A.

August 2015

The inhibitory effect of Lactobacillus sakei KBL isolated from kimchi on the adipogenesis of 3T3-L1 cells

Abnormal adipocyte growth, in terms of increased cell numbers and increased cell differentiation, is considered to be a major pathological feature of obesity. Thus, the inhibition of preadipocyte mitogenesis and differentiation could help prevent and suppress obesity. Some probiotics and cellular components are known to modulate the lipid metabolism in vitro and/or in vivo. The aim of this study was to investigate...

Author(s): KiBeom Lee and GunSu Jeon

August 2015

Phylogenetic study on Microcotyle sp. (Monogenea) from common dentex (Dentex dentex) in the Mediterranean Sea, Greece

The monogenean Microcotyle sp. was isolated from common dentex in the Sea of Crete, the part of the Mediterranean Sea. The 28S rRNA gene of Microcotyle sp. was amplified by polymerase chain reaction (PCR). The PCR product was sequenced and compared with other 28S rRNA sequences of Monogenea. Phylogenetic analyses were performed with neighbour-joining (NJ), minimum evolution (ME), maximum likelihood (ML) and maximum...

Author(s): Jin Woo Jun

August 2015

Status of cassava mosaic disease and whitefly population in Zambia

Cassava mosaic disease is the most important disease affecting cassava in Zambia. A study was conducted through a survey to determine the status of cassava mosaic disease incidence, severity and whitefly abundance in farmers’ fields in six provinces: Lusaka, Northern, North-Western, Luapula, Eastern and Western between March and May 2014. The study reveals that cassava mosaic disease incidence was highest in...

Author(s): P. C. Chikoti, M. Tembo, M. Chisola, P. Ntawuruhunga and J. Ndunguru

August 2015

Polymers selection for a liquid inoculant of Azospirillum brasilense based on the Arrhenius thermodynamic model

Plant growth promoting bacteria (PGPB) enhances the growth of their hosts and can protect them from biotic and abiotic stresses. Bacterial inoculants contain one or more of these beneficial strains in a carrier material, which must be able to maintain the viability of the cells during the time of storage, and also guarantee the biological activity of the strains once applied in the soil. These inoculants can be solid,...

Author(s): Cortés-Patiño Sandra and Bonilla Ruth Rebeca

August 2015

Biosynthesis of silver nanoparticles by plants crude extracts and their characterization using UV, XRD, TEM and EDX

Plant extracts are very cost effective and eco-friendly, thus, can be an economic and efficient alternative for the large-scale synthesis of nanoparticles. The preparation of stable, uniform silver nanoparticles by reduction of silver ions with Emblica officinalis, Terminalia catappa and Eucalyptus hybrida extract is reported in the present paper. It is a simple process of global research interest for obtaining silver...

Author(s): Vishwajeet Singh, Ankita Shrivastava and Nitin Wahi

August 2015

Biochemical profile of cuttings used for in vitro organogenesis of Prunus africana: An endangered species in Cameroon

Prunus africana (Kanda stick) is a valuable medicinal tree classified as priority species for domestication in Cameroon. To measure the ability for developing axillary buds and multiple shoots in relation with the origin and state of explants, some biochemical parameters were assessed in eight types of single-node cuttings. These cuttings were treated with different concentrations of kinetin (kin) indole butyric acid...

Author(s): Justine Germo Nzweundji, Nicolas Niemenak, Doungous Oumar, Julie Judith Tsafack, Koffi Konan, Leopold Nyochembeng, Christelle Ngamegne Noumi, Nehemie Donfagsiteli Tchinda, and Denis Omokolo Ndoumou

August 2015

Liver histopathology in bovine Fascioliasis

The livers of slaughtered cattle were examined by visualization, palpation and incision. Macroscopically, some of the infected livers appeared to be slightly swollen with pale color at the round edges, while some appeared greatly swollen, with a few small irregular whitish areas indicating fibrosis over the parietal surface. In some cases, the capsule was thick and rough with whitish or reddish discoloration and...

Author(s): Okoye, I. C., Egbu, F. M. I., Ubachukwu, P. O and Obiezue, Nduka R.

August 2015

Assessing the genetic diversity of 48 groundnut (Arachis hypogaea L.) genotypes in the Guinea savanna agro-ecology of Ghana, using microsatellite-based markers

Groundnut (Arachis hypogaea L.) is the most important grain legume in Ghana. However, its production is constrained by a myriad of biotic and abiotic stresses which necessitate the development and use of superior varieties for increased yield. Germplasm characterisation both at the phenotypic and molecular level is important in all plant breeding programs. The aim of this study was to characterise selected advanced...

Author(s): Richard Oteng-Frimpong, Mandla Sriswathi, Bonny R Ntare and Felix D. Dakora

August 2015

Effects of Moringa oleifera leaf extract on morphological and physiological growth of cassava and its efficacy in controlling Zonocerus variegatus

Zonocerus variegatus infestation has been found to cause a lot of devastation in the production of cassava crop plants in tropical and subtropical areas. Understanding the efficacy of Moringa oleifera leaf extract on controlling Z. variegatus is desired. The present study was conducted in the Department of Crop Science experimental site, Faculty of Agriculture, University of Nigeria, Nsukka to investigate the effects of...

Author(s): Uchenna Mabel Ndubuaku, Theophilus Chinyere Nkasiobi Ndubuaku, Emmanuel Ike and Peter Ikemefuna Ezeaku

August 2015

Anti-nutrient, vitamin and other phytochemical compositions of old and succulent moringa (Moringa oleifera Lam) leaves as influenced by poultry manure application

The study was carried out to determine the anti-nutrient, vitamin and other phytochemical compositions of old and succulent leaves of Moringa oleifera plants as influenced by poultry manure application. Three levels of poultry manure, that is, 0, 5 and 10 tonnes ha-1 were used for treatment. Poultry manure application insignificantly (p > 0.05) increased oxalate, phytate and saponin compositions of the leaves. The...

Author(s): Uchenna Mabel Ndubuaku, Nwankwo Vincent Uchenna, Kayode Paul Baiyeri and Juliana Ukonze

August 2015

Effects of ethylene inhibitors, silver nitrate (AgNO3), cobalt chloride (CoCl2) and aminooxyacetic acid (AOA), on in vitro shoot induction and rooting of banana (Musa acuminata L.)

Significant increase in shoot regeneration, leaf chlorophyll content and rooting occurred when silver nitrate (AgNO3), cobalt chloride (CoCl2) or aminooxyacetic acid (AOA) were added to banana culture medium. The highest numbers of shoots per explants shoot length and leaf surface area was obtained when media were supplemented with 10 mgl-1 AgNO3. Number of shoots per explants increased three fold, shoot length and leaf...

Author(s): Samih M. Tamimi

August 2015

Bacteriocinogenic potential and genotypic characterization of three Enterococcus faecium isolates from Algerian raw milk and traditional butter

The detection of bacteriocinogenic potential of Enterococcus sp. isolates from Algerian raw milk coded LO4 and LO12 and from traditional butter coded BRO2 was carried on M17 buffered medium. PCR amplification of Enterococcus sp. DNA using specific enterococcal primers gave 733 bp fragments. The phylogenetic analysis using the neighbour joining method further supported the identification of the three strains as...

Author(s): Louiza Lazreg, Fatiha Dalache, Halima Zadi-Karam and Nour-Eddine Karam

August 2015

Multiple protein-domain conservation architecture as a non-deterministic confounder of linear B cell epitopes

Epitope prediction is a critical step to diagnostic and vaccine discovery. Despite existence of some parameters for epitope discovery, this area remains inconclusive and wanting-for new complementary or stand-alone tools. The phenomenon of multiple protein-domain conservation architecture (MPDCA) as used here refers to homologous motifs unveiled by multiple sequence alignments across strain-variants of the same protein...

Author(s): Misaki Wayengera

August 2015

Agrobacterium mediated genetic transformation and regeneration in elite rice (Oryza sativa L.) cultivar BRRI dhan56

Agrobacterium-mediated genetic transformation of rice (Oryza sativa L.) cultivar BRRI dhan56 was carried out in this study. Agrobacterium tumefaciens strain LBA 4404, which harbors the plasmid pIG121 that carries the genes for ß-glucuronidase gene, served as a reporter gene in the histochemical assay and the neomycin phosphotransferase ΙΙ (NPT ΙΙ) gene for the identification of resistance to...

Author(s): Md. Mahmudul Islam, Zahida Yesmin Roly, Mst. Zannatun Naim and Md. Khalekuzzaman

August 2015

Impact of organic and inorganic fertilizers on growth, fruit yield, nutritional and lycopene contents of three varieties of tomato (Lycopersicon esculentum (L.) Mill) in Ogbomoso, Nigeria

Field experiments were carried out to assess the impact of 100% NPK fertilizer recommendation (300 kg NPK 20:10:10), 100% Tithonia compost (TC), 75% NPK + 25% TC, 50% NPK + 50% TC, 25% NPK + 75% TC and control (non-fertilized plant) on the growth, fruit yield, nutritional and lycopene contents of three tomato varieties (Raoma VF; Ogbomoso local and Califonia wonder). The experiment was a split plot fitted into a...

Author(s): Ilupeju, E. A. O, Akanbi, W. B., Olaniyi, J. O., Lawal, B. A., Ojo M. A. and Akintokun, P. O.

August 2015

Physiological and enzymatic changes in rice seeds stored at low temperatures

This study aimed to evaluate the effect of low temperatures on the physiological and enzymatic changes of rice seeds. The seeds were packed in airtight chambers and maintained at temperatures of 8 and -50°C for periods of 15, 30 , 45, 60, 75 and 90 days. The same procedure was adopted for the control treatment with the seeds kept at a temperature of 25°C. The seeds were evaluated regarding germination test;...

Author(s): Raimundo Wagner de Sousa Aguiar, Deyvid Rocha Brito, Magnólia de Mendonca Lopes, Gil Rodrigues dos Santos, Clovis Maurilio Sousa, Ezequiel Marcelino Marcelino da Silva and Julcemar Didonet

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