African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12296

AJB Articles

Suppressive effects of a polymer sodium silicate solution on powdery mildew and root rot diseases of miniature rose

October 2015

Sodium silicate was dissolved in water in either a monomer form or polymer form; the effects of both forms of sodium silicate aqueous solution on rose powdery mildew and root rot diseases of miniature rose were examined. Both forms of sodium silicate aqueous solution were applied to the roots of the miniature rose. Potassium silicate aqueous solution was used as a control and was compared to the effect of sodium...

Author(s): Mohsen Mohamed Elsharkawy, Tatsuya Hase, Masafumi Shimizu and Mitsuro Hyakumachi

Toxic effects of Ricinus communis non-protein trypsin inhibitor on Spodoptera frugiperda (J. E. Smith) (Lepidoptera:Noctuidae)

October 2015

In the study reported herein, we aimed to isolate a trypsin inhibitor from Ricinus communis leaves through chromatographic and spectrometric techniques and evaluate its toxic effects on the development of Spodoptera frugiperda larvae. Plant extracts were submitted to fractionation in adsorption column. The fraction 10, which showed the highest inhibitory activity, were incorporated into an artificial diet at the...

Author(s): Gislaine A. Carvalho, Custódio D. dos Santos, Dejane S. Alves, Geraldo A. Carvalho, Maria das G. Cardoso and Marcelo M. de Haro

The efficacy of palm oil sludge in reducing ruminal methanogenesis using rumen simulation technique

October 2015

The effect of including palm oil sludge in cattle diet (silage) was conducted in vitro in order to ascertain the suppressing effect on ruminal methanogenesis. The silage used for the study was Panicum maximum, Centrocema pubscens, Bracharia decumbens and Andropogon gayanus. The silage were cut into an average size of 3 to 5 cm and ensiled for 21 days. Four treatment diets were formulated with the silage by including...

Author(s): L. C. Ugwuowo, A. G. Ezekwe, A. O. Ani, S. I. Eze, C. N. Anyanwu and A. Ofomatah  

Heterogeneous expression and functional evaluation of in silico characterized recombinant OmpC of Salmonella Typhimurium as a functional poultry vaccine to eradicate zoonotic transmission

October 2015

Salmonellosis is one of the major global health concerns leading to millions of deaths annually. The present vaccines not being up to the mark necessitate the need for the development of new generation vaccines. Outer membrane proteins (Omps) of several Gram negative bacteria have been investigated and found to be immunogenic and protective. The present study explores the potential of a major porin protein (OmpC) of...

Author(s): Richa Jha, Anil Kumar, Anjani Saxena, Mamta Pandey, Rajesh Kumar and Mumtesh Kumar Saxena

Use of simple sequence repeat (SSR) markers for screening blue disease resistance in cotton germplasm exchange

October 2015

Blue disease of cotton is an economically important disease of the crop first described from the Central African Republic and spread to other countries. Brazil and other South American countries record crop losses of up to 80% from infection but no cases of the disease have been reported in Tanzania. Resistance to the disease has been found in African germplasm and transferred to crop cultivars worldwide. Molecular...

Author(s): Christopher Faustine, Lucia Vieira Hoffmann, Flora Ismail Tibazarwa and Everina Lukonge

Antibiosis in Ascia monuste orseis Godart (Lepidoptera: Pieridae) caused by kale genotypes

October 2015

Ascia monuste orseis (Lepidoptera: Pieridae) is one of the main insect pests of kale. The study was done to identify kale varieties resistant to A. monuste orseis by the antibiosis resistance mechanism. Kale genotypes (26) were evaluated in experiments performed at the Laboratory of Agricultural Entomology of Goiano Federal Institute - Campus Urutaí. A completely randomized experimental design with 50 replicates...

Author(s): Luciano Nogueira, Lígia Alves de Paiva, Andre Cirillo de Sousa Almeida, Zulene Antonio  Ribeiro Arlindo Leal Boica Junior and Flavio Goncalves de Jesus

Estimation of genetic diversity between three Saudi sheep breeds using DNA markers

October 2015

The genetic variation of Najdi, Harri and Awassi breeds of Saudi sheep prevailing in Raniah province of Makka district were assessed and compared to Sudanese Desert sheep using random amplified polymorphic DNA polymerase cahin reaction (RAPD-PCR) technique. Five primers successfully amplified distinguishable 40 bands with an average of 96% polymorphism revealing that Saudi sheep breeds possess the needed genetic...

Author(s): Ahmed Abdel Gadir Adam, Nada Babiker Hamza, Manal Abdel Wahid Salim and Khaleil Salah Khalil

Determination of lactic acid bacteria in Kaşar cheese and identification by Fourier transform infrared (FTIR) spectroscopy

October 2015

Lactic acid bacteria (LAB) arise in Kaşar cheese, an artisanal pasta filata cheese produced in Turkey from raw milk without starter addition or pasteurized milk with starter culture. In this study, 13 samples of Kaşar cheese that were produced from raw milk were used as reference materials. LAB were characterized by using phenotypic, API and Fourier transform infrared (FTIR) spectroscopy methods. One hundred and...

Author(s): İlkay Turhan and Zübeyde Öner

Profile, antioxidant potential, and applicability of phenolic compounds extracted from Spirulina platensis

October 2015

This work aimed at an investigation of the profile of free phenolic compounds (PC) of Spirulina platensis and assesses their antioxidant potential, applying them as a natural conservative in minimally processed apples. The phenolic extract showed 396 µg g-1 gallic acid, 347  µg g-1 of caffeic acid, 54 µg g-1 salicylic acid and 3.5 µg g-1 trans-1-cinmamic a total of 608 µg PC g-1 of S....

Author(s): Taiana Denardi de Souza, Luciana Prietto, Michele Moraes de Souza and Eliana Badiale Furlong

Analysis of bioactive chemical components of two medicinal plants (Coriandrum sativum and Melia azedarach) leaves using gas chromatography-mass spectrometry (GC-MS)

October 2015

The main objective of this study was to determine the phytochemical composition in the leaves of Coriandrum sativum, using methanolic extraction and report the main functional components by using IR technique. The phytochemical compounds in the extract were then screened by GC-MS method. Seven bioactive phytochemical compounds were identified in the methanolic extract of C. sativum: 1,6-octadien-3-ol, 3,7-dimethyl,...

Author(s): Ali Hussein Al-Marzoqi, Imad Hadi Hameed and Salah Ali Idan

In vitro regeneration of Calophyllum brasiliense Cambess: A valuable medicinal tree

October 2015

Calophyllum brasiliense (Calophyllaceae) is a medicinal tree known mainly for producing calanolides, secondary metabolites against HIV-1 reverse transcriptase. This wild plant is listed as threatened and despite its outstanding medicinal value, no studies have been conducted on its propagation or preservation. This study standardized a procedure for the micropropagation of C. brasiliense with nodal segments from in...

Author(s): A. Maldonado-Magana, A. Bernabe-Antonio, E. Salcedo-Perez and F. Cruz-Sosa

Gene mining a marama bean expressed sequence tags (ESTs) database: Embryonic seed development genes and microsatellite marker identification

October 2015

Tylosema esculentum (marama bean) is one of the underutilized legumes that have potential to provide protein and fatty acids to ensure food security in dry parts of Southern Africa. In order to establish rapid domestication programs for the plant, it is important to explore the plant’s genome and identify functional genes molecular markers like microsatellites in order to develop molecular tools. With the advent...

Author(s): Emilia N. Sheehama and Percy M. Chimwamurombe

Multiploidy occurrence in tomato calli from anther culture

October 2015

Anther culture has long been used for the production of fully homozygous lines in order to produce, mainly, doubled haploid plants, which are of great interest in plant breeding. For tomato, a recalcitrant species for androgenesis production protocols have not been standardized. It is known that the genotype, anther size, the developmental stage of the microspore, and the medium composition are some factors that can...

Author(s): Sirlei Aparecida Julião, Carlos Roberto Carvalho, Thaís Cristina Ribeiro da Silva and Andréa Dias Koehler

Extraction and physicochemical characterization of a potential multifunctional pharma-excipient from crab shell wastes

October 2015

An efficient and beneficial waste disposal mechanism is highly desired. This study was carried out to extract and characterize a potential multifunctional pharmaceutical excipient from crab shell wastes. Shells of Pachygrapsus mamoratus were obtained from Oron, a coastal town in Akwa Ibom State of Nigeria. Chitin was extracted from the powdered shell by deproteination and demineralization; and chitosan was derived by...

Author(s): Emmanuel O. Olorunsola, Olubunmi J. Olayemi, Stephen O. Majekodunmi and Unyime B. Etukudo

A protocol for Agrobacterium-mediated transformation of Kalanchoë blossfeldiana with a flavonoid 3',5' hydroxylase (F3'5'H) gene

September 2015

In the present investigation, explants from Kalanchoë blossfeldiana were used for gene transformation. The young leaves were inoculated with Agrobacterium tumefaciens LBA4404 strain with a binary vector plasmid pArtblue containing F3'5'H gene under control of CaMV35S promoter and nptII selectable marker gene. After inoculation, the explants were transferred to the co-cultivation medium. They were then...

Author(s): Phopgao Buddharak, Ruttaporn Chundet and Warut U-kong

Effect of arbuscular mycorrhizal fungal inoculation on growth, and nutrient uptake of the two grass species, Leptochloa fusca (L.) Stapf and Sporobolus robustus Kunth, under greenhouse conditions

September 2015

The aim of our work was to assess the effect of inoculation with three arbuscular mycorrhizal fungi (AMF) (Rhizoglomus aggregatum (N.C. Schenck and G.S. Sm.) Sieverd., G.A. Silva and Oeh., Funneliformis mosseae (T.H. Nicolson and Gerd.) C. Walker and A. Schüssler. and Rhizoglomus intraradices (N.C. Schenck and G.S. Sm.) Sieverd., G.A. Silva and Oehl.), and a mixed inoculum of these AMF on root colonization, biomass...

Author(s): Fatoumata Fall,, Diégane Diouf,, Dioumacor Fall,, Ibrahima Ndoye,, Cheikh Ndiaye, Aboubacry Kane, and Amadou Mustapha Bâ,

Cooking and drying processes optimization of Pentadesma butyracea kernels during butter production

September 2015

The cooking and the drying of the Pentadesma butyracea kernels are the main steps in the traditional method of extracting P. butyracea butter. In this work, the Response Surface Method with Central composite design was used to optimize the cooking and drying of Pentadesma kernels during P. butyracea butter production. The cooking and drying times, as well as the drying temperature of Pentadesma kernels are considered as...

Author(s): Eric Badoussi, Paulin Azokpota, Yann E. Madodé, Fidèle P. Tchobo, Balbine Fagla Amoussou, Adéchola P. P. Kayodé, Mohamed M. Soumanou and D. Joseph Hounhouigan

Use of pectin in the storage of mangaba fruits (Hancorniaspeciosa Gomes) associated with refrigeration

September 2015

The objective of this study was to evaluate the storage potential of mangaba fruits coated with pectin biofilm stored in biochemical oxygen demand (BOD) (22 ± 1°C) under modified atmosphere. Scanning electron microscopy (SEM) was used to analyze the physical and chemical changes, vitamin C content, soluble solid, titratable acidity and turgor pressure, and colour and physical structure of the fruits during...

Author(s): Plácido, G. R., Silva, R. M., Cagnin, C.,Silva, M. A. P., Soares, J. C. and Caliari, M.

Fermentable sugars and microbial inhibitors formation from two-stage pretreatment of corn stalk with variation in particle size and severity factor

September 2015

Microbial inhibitors including weak acids, furan derivatives and phenolic compounds are key problems of cellulosic bio-fuels production by fermentation. Most of these inhibitors are sugars and lignin degradation compounds, which are almost unavoidable during pretreatment processes. While, most of the one stage pretreatment has been conducted at high severity factors of 3.5 or more to get high sugar yield, with increase...

Author(s): Nawa Raj Baral, Jianzheng Li and Ajay Kumar Jha

Genetic variability of indigenous cowpea genotypes as determined using inter-simple sequence repeats markers

September 2015

Cowpea [Vigna unguiculata (L.) Walp.] is cultivated widely by small farmers in the semiarid region of Northeastern Brazil for subsistence purposes, especially to complement the family income. However, owing to the limited availability of water in this region, there is an urgent need for novel highly productive drought-tolerant cultivars. The aim of the present study was to establish the genetic variability of 14 cowpea...

Author(s): R. F. M. Mendes, F. B. Britto, F. R. Freire Filho and P. S. C. Lima

Preliminary characterization of residual biomass from Hibiscus sabdariffa Calyces

September 2015

Hibiscus sabdariffa calyces are mainly used for different agro-food and beverages applications. The residual biomass generated contains various useful substances that were extracted and characterized. It contained 23% (w/w) soluble pectic material, a food additive, extracted with hot acidified water (80°C, pH = 1.5) and precipitated with ethanol. The molecular weight (28.5 and 109.7 kDa), the degree of methylation...

Author(s): Cheikh Beye, , Mario Aguedo, Serge Hiligsmann, Lat Souk Tounkara, Aurore Richel and Philippe Thonart

A study of the optimal conditions for glucoamylases obtained from Aspergillus niger using amylopectin from cassava starch as carbon source

September 2015

A fourteen day pilot study carried out showed that high glucoamylase activities were obtained on the 4 and 11th day of fermentation and the enzymes were harvested on the respective days giving the codes GluAgCSV4 and GluAgCSV11. The optimal pH and optimal temperatures for enzyme activities GluAgCSV4 and GluAgCSV11 were in a range of 6 to 7 and 50 to 55, using cassava, guinea corn and tiger nut starch as substrates,...

Author(s): Ezugwu, A. L., Eze, S. O. O. and Chilaka, F. C.

Anatomical and histochemical analysis of vegetative organs of Vernonia ferruginea Less. (Asteraceae)

September 2015

Vernonia ferruginea Less. is a perennial shrub species, present in several regions of Brazil, especially in the savanna. It is popularly used as a phytotherapic. This fact justifies the need to anatomically characterize the plant for its accurate identification and to conduct histochemical studies with the aim of identifying the chemical nature of its cellular constituents. The species-specific data will contribute...

Author(s): Oliveira, R. C. de, Vasconcelos Filho, S. C., Bastos, A. V. S., Vasconcelos, J. M. and Rodrigues, A. A.

Chemical composition and mutagenic assessment of petrochemical effluents on onion (Allium cepa) root tip mitosis

September 2015

The continous production and release of chemicals into the environment have led to the need to assess their chemical composition and genotoxic effects on cell reproduction. Two petrochemicals, air liquid and polyester resin effluents were assessed. The common onion of the purple variety was used as the test organism. The results of the chemical analysis of the wastewaters showed high concentration of some potentially...

Author(s): Abu, Ngozi E., Asomba, C. O. and Ubani, C.

Management of Fusarium Wilt using mycolytic enzymes produced by Trichoderma harzianum (Th. Azad)

September 2015

The main aim of this study was to isolate the best chitinase and glucanase enzyme producing Trichoderma strain to manage the Fusarium wilt disease of Cicer aritenum under in vitro conditions. We also studied the effect of Trichoderma strains on the growth and development of C. aritenum plants. Seven strains of Trichoderma were screened against the Fusarium pathogen to isolate the best biocontrol agent causing maximum...

Author(s): Mohammad Shahid, Mukesh Srivastava, Sonika Pandey, Vipul Kumar, Anuradha Singh, Shubha Trivedi, Y. K. Srivastava and Shiv Ram

β-Lactam and chloramphenicol-resistant enterobacteria in hospital surfaces

September 2015

The following study aimed to research the Enterobacteriaceae present on the material surfaces of a hospital environment in a Community Health Unit in Ceara-Brazil. Data was collected in 10 different rooms and facilities by rubbing sterile swabs in an enclosed area of 10 cm² for a minute. Bacterial growth was observed in all surveyed areas. However, Enterobacteriaceae were only found in surfaces from the kitchen and...

Author(s): Jean Linhares de Lima, Sara Edwirgens Costa Benicio Vasconcelos, Theodora Thays Arruda Cavalcante, and Renata Albuquerque Costa,

A direct and sensitive method for screening fructooligosaccharides-digesting microorganisms useful in food and health science

September 2015

Short-chain fructooligosaccharides (FOS) stimulate selectively the growth and activity of microorganisms in the colon providing positive health effects and well-being in humans and animals. The lack of accurate isolation methods, however, hampers the possibility of getting new potential fructo-oligosaccharides (FOS)-fermenting yeast or bacterial strains. A valuable screening procedure to visually detect bacterial and...

Author(s): Trujillo L. E., Granda S., Ordoñez M. I., Ramos T. I. and Avalos R. 

Nanoparticle (MPG)-mediated delivery of small RNAs into human mesenchymal stem cells

September 2015

The cellular membrane constitutes an effective barrier that protects the complex, yet highly ordered, intracellular compartment of the cell. Passage of molecules across this barrier is highly regulated and highly restricted. Cell penetrating peptides (CPPs) are a class of small cationic peptides that are able to defy the rules of membrane passage and gain access to the intracellular environment. MPG is one member of...

Author(s): Nnaemeka Darlington Ndodo,

Development of stable marker-free nuclear transformation strategy in the green microalga Chlorella vulgaris

September 2015

Although microalgae have valuable resources with great necessity in many biotechnological applications, no tools have been developed yet for a stable genetic transformation without antibiotic marker genes in these organisms. Chlorella is one of the most useful genus for biotechnological applications. The transfer of foreign DNA (vector or linear DNA cassette) into Chlorella by electroporation has very low stability and...

Author(s): Ahmed E. Gomma#, Sung-Kwon Lee#, Sang Mi Sun, Seung Hwan Yang, and Gyuhwa Chung

Screening of the kernels of Pentadesma butyracea from various growing sites of Benin and evaluation of their antioxidant pigments content

September 2015

Pentadesma butyracea Sabine (Clusiaceae) is a ligneous forest species of multipurpose uses. It is widely distributed in Africa from Guinea-Bissau to the West of the Democratic Republic of Congo. This study screened the kernel of P. butyracea on the basis of their physico-chemical properties. Six types of kernels were distinguished. The plant producing type 1 kernels (with medium length, low width and high thickness) and...

Author(s): Bernolde P. Ayegnon, Adéchola P. P. Kayodé, Glawdys Gnanvi, Yann madodé, Fagla-Amoussou Balbine, Paulin Azokpota, Mohamed M. Soumanou and Joseph D. Hounhouigan

Essential oil composition of different fractions of Piper guineense Schumach. et Thonn from Cameroon using gas chromatography-mass spectrometry and their insecticidal effect on Sitophilus oryzae (L.)

September 2015

Essential oil fractions from dried seed powder of Piper guineense were analyzed by gas chromatography-mass spectrometry (GC-MS) and evaluated for their insecticidal effects on Sitophilus oryzae L. The GC-MS analysis showed quantitative and qualitative differences between the oil fractions. Chromatographic results revealed chemical constituents like eugenol, piperanol, pinene, carene, copaene with insecticidal...

Author(s): Esther Obi Oben⊃, Robyn McConchie⊃, Kim Phan-Thien⊃ and Nelson Neba Ntonifor⊃

Heritability of polyphenols, anthocyanins and antioxidant capacity of Cameroonian cocoa (Theobroma cacao L.) beans

September 2015

This study investigates the heritability of polyphenolic, anthocyanin and antioxidant capacity of beans derived from four cocoa clones and their offsprings. These compounds were analyzed at 280 nm (polyphenols) and 520 nm (anthocyanins) by reversed-phase high-performance liquid chromatography (RP-HPLC) method using a photodiode array detector (PDA). The antioxidant capacity of methanolic extracts prepared from cocoa...

Author(s): Pierre Effa Onomo, Nicolas Niemenak, Pierre François Djocgoue, Martine Louise Ondobo and Denis Omokolo Ndoumou

Inflorescence bulbils of tiger lily in vivo and bulbils culture in vitro

September 2015

The present study includes two experiments; the physiological and the biotechnological experiments. The physiological experiment was designed to investigate the most interesting rare natural phenomenon of the development of vegetative reproductive organs (bulbils) in the inflorescence of tiger lily. In the other hand, the biotechnological experiment was carried out to evaluate the regeneration potential of bulbils...

Author(s): Hassan M. Asker

Effects of different culture conditions (photoautotrophic, photomixotrophic) and the auxin indole-butyric acid on the in vitro acclimatization of papaya (Carica papaya L. var. Red Maradol) plants using zeolite as support

September 2015

Plant regeneration of papaya via organogenesis and somatic embryogenesis has been successful; however, the biggest problem of in vitro culture of this species is the acclimatization of regenerated plants, where over 70% of the plants are lost before being planted in the field. Decreasing the relative humidity inside the culture vessel and thus increasing the ventilation, appears to have a greater effect on the...

Author(s): Laisyn Posada Pérez, Yenny Padrón Montesinos, Justo González Olmedo, Romelio Rodríguez Sánchez, Osvaldo Norman Montenegro, Raul Barbón Rodriguez, Ortelio Hurtado Ribalta, Rene Carlos Rodriguez Escriba, Dion Daniels and Rafael Gómez-Kosky

The effects of exotic weed Flaveria bidentis with different invasion stages on soil bacterial community structures

September 2015

A new exotic weed, Flaveria bidentis, is spreading in central China where it forms dense monospecific patches modifying the structure of different native ecosystems and threatening native aboveground biodiversity. However, little is known about the consequences of such an invasion for soil bacterial community, especially its effect pattern at different invasion stages. In this study, soil samples were taken in native...

Author(s): Chaohe Huangfu, Huiyan Li, Xinwei Chen, Hongmei Liu and Dianlin Yang

Extraction and characterization of Retama monosperma fibers

September 2015

The aims of this study were to determine the good conditions for fibers extraction from Retama monosperma young stems and their mechanical, physical and chemical characteristics. The fibers were extracted using a range of NaOH concentration from 1 to 16% in a period of treatment of 1 to 24 h, coupled with a physical treatment. For the evaluation of physico-mechanical characteristics, 200 samples were performed in the...

Author(s): AIZI Djamel Eddine and KAID HARCHE Meriem

Analysis of the genetic diversity of selected East African sweet potato (Ipomea batatas [L.] Lam.) accessions using microsatellite markers

August 2015

Sweet potato (Ipomea batatas [L.] Lam.) is an economically important crop in East Africa chiefly grown by small holder farmers. Sharing of vines for planting is a very common occurrence among these farmers and eventually varieties are given local names, making it hard to trace the original pedigree. It is therefore important to characterise the sweet potato germplasm for purposes of breeding and germplasm conservation....

Author(s): Ochieng, L. A., Githiri, S. M., Nyende, B. A., Murungi, L. K., Kimani, N. C., Macharia, G. K. and Karanja, L.

Impact of the application of humic acid and sodium nitroprusside on nickel toxicity: Analysis of relative gene expression

August 2015

Nickel (Ni) is an essential micronutrient for plants but in high concentrations may turn toxic. This paper discusses the potential role of humic acid (HA) and sodium nitroprusside in modulating or preventing oxidative stress in rice plants. Three genes [superoxide dismutase (SOD) glutathione reductase (GR) and ascorbate peroxidase (APx) were selected for an expression study using a real time PCR technique. Three...

Author(s): Sandra Perez Alvarez, Daniel Cabezas Montero, Norma Elena Leyva Lopez, Jesús Mendez Lozano and Esteban Sanchez Chavez

Screening of successive extracts of Amorphophallus konjac for antibacterial activity

August 2015

Amorphophallus is an Aroid family member, native to Asia. Amorphophallus konjac K. Koch ex N.E.Br. is also known as snake plant due to snake like outlines on its stem. In Mount Abu the plant is grown in wild and known for its toxic principles. In Traditional Chinese System of Medicine (TCM), it was mentioned that gel prepared from flour has been used in detoxification, tumor suppression, phlegm liquefaction and skin...

Author(s): Adnan Khokar and Ekta Menghani

Lipid lowering and anti-atherosclerotic properties of Tinospora crispa aqueous extract on high-cholesterol diet-induced hyperlipidemic rabbits

August 2015

This study was aimed to investigate the hypocholestrolemic and anti-atherosclerotic properties of Tinospora crispa aqueous extract (TCAE) on rabbits for 10 weeks. The hyperlipidemic rabbits were induced and the rabbit were given different concentration of TCAE (200, 450 and 600 mg/kg). Results from lipid analysis show that the level of total cholesterol (TC), triglyceride (TG) and LDL-C on the hyperlipidemic rabbits...

Author(s): Zamree, M. S., Ihsan Safwan, K., Khairul Kamilah A. K., Mohd Kamal N. H., Rasadah, M. A., Mohd Shahidan M. A., Daryl, J. A. and Zulkhairi, A.

The inhibitory effect of Lactobacillus sakei KBL isolated from kimchi on the adipogenesis of 3T3-L1 cells

August 2015

Abnormal adipocyte growth, in terms of increased cell numbers and increased cell differentiation, is considered to be a major pathological feature of obesity. Thus, the inhibition of preadipocyte mitogenesis and differentiation could help prevent and suppress obesity. Some probiotics and cellular components are known to modulate the lipid metabolism in vitro and/or in vivo. The aim of this study was to investigate...

Author(s): KiBeom Lee and GunSu Jeon

Phylogenetic study on Microcotyle sp. (Monogenea) from common dentex (Dentex dentex) in the Mediterranean Sea, Greece

August 2015

The monogenean Microcotyle sp. was isolated from common dentex in the Sea of Crete, the part of the Mediterranean Sea. The 28S rRNA gene of Microcotyle sp. was amplified by polymerase chain reaction (PCR). The PCR product was sequenced and compared with other 28S rRNA sequences of Monogenea. Phylogenetic analyses were performed with neighbour-joining (NJ), minimum evolution (ME), maximum likelihood (ML) and maximum...

Author(s): Jin Woo Jun

Status of cassava mosaic disease and whitefly population in Zambia

August 2015

Cassava mosaic disease is the most important disease affecting cassava in Zambia. A study was conducted through a survey to determine the status of cassava mosaic disease incidence, severity and whitefly abundance in farmers’ fields in six provinces: Lusaka, Northern, North-Western, Luapula, Eastern and Western between March and May 2014. The study reveals that cassava mosaic disease incidence was highest in...

Author(s): P. C. Chikoti, M. Tembo, M. Chisola, P. Ntawuruhunga and J. Ndunguru

Polymers selection for a liquid inoculant of Azospirillum brasilense based on the Arrhenius thermodynamic model

August 2015

Plant growth promoting bacteria (PGPB) enhances the growth of their hosts and can protect them from biotic and abiotic stresses. Bacterial inoculants contain one or more of these beneficial strains in a carrier material, which must be able to maintain the viability of the cells during the time of storage, and also guarantee the biological activity of the strains once applied in the soil. These inoculants can be solid,...

Author(s): Cortés-Patiño Sandra and Bonilla Ruth Rebeca

Biosynthesis of silver nanoparticles by plants crude extracts and their characterization using UV, XRD, TEM and EDX

August 2015

Plant extracts are very cost effective and eco-friendly, thus, can be an economic and efficient alternative for the large-scale synthesis of nanoparticles. The preparation of stable, uniform silver nanoparticles by reduction of silver ions with Emblica officinalis, Terminalia catappa and Eucalyptus hybrida extract is reported in the present paper. It is a simple process of global research interest for obtaining silver...

Author(s): Vishwajeet Singh, Ankita Shrivastava and Nitin Wahi

Biochemical profile of cuttings used for in vitro organogenesis of Prunus africana: An endangered species in Cameroon

August 2015

Prunus africana (Kanda stick) is a valuable medicinal tree classified as priority species for domestication in Cameroon. To measure the ability for developing axillary buds and multiple shoots in relation with the origin and state of explants, some biochemical parameters were assessed in eight types of single-node cuttings. These cuttings were treated with different concentrations of kinetin (kin) indole butyric acid...

Author(s): Justine Germo Nzweundji, Nicolas Niemenak, Doungous Oumar, Julie Judith Tsafack, Koffi Konan, Leopold Nyochembeng, Christelle Ngamegne Noumi, Nehemie Donfagsiteli Tchinda, and Denis Omokolo Ndoumou

Liver histopathology in bovine Fascioliasis

August 2015

The livers of slaughtered cattle were examined by visualization, palpation and incision. Macroscopically, some of the infected livers appeared to be slightly swollen with pale color at the round edges, while some appeared greatly swollen, with a few small irregular whitish areas indicating fibrosis over the parietal surface. In some cases, the capsule was thick and rough with whitish or reddish discoloration and...

Author(s): Okoye, I. C., Egbu, F. M. I., Ubachukwu, P. O and Obiezue, Nduka R.

Assessing the genetic diversity of 48 groundnut (Arachis hypogaea L.) genotypes in the Guinea savanna agro-ecology of Ghana, using microsatellite-based markers

August 2015

Groundnut (Arachis hypogaea L.) is the most important grain legume in Ghana. However, its production is constrained by a myriad of biotic and abiotic stresses which necessitate the development and use of superior varieties for increased yield. Germplasm characterisation both at the phenotypic and molecular level is important in all plant breeding programs. The aim of this study was to characterise selected advanced...

Author(s): Richard Oteng-Frimpong, Mandla Sriswathi, Bonny R Ntare and Felix D. Dakora

Effects of Moringa oleifera leaf extract on morphological and physiological growth of cassava and its efficacy in controlling Zonocerus variegatus

August 2015

Zonocerus variegatus infestation has been found to cause a lot of devastation in the production of cassava crop plants in tropical and subtropical areas. Understanding the efficacy of Moringa oleifera leaf extract on controlling Z. variegatus is desired. The present study was conducted in the Department of Crop Science experimental site, Faculty of Agriculture, University of Nigeria, Nsukka to investigate the effects of...

Author(s): Uchenna Mabel Ndubuaku, Theophilus Chinyere Nkasiobi Ndubuaku, Emmanuel Ike and Peter Ikemefuna Ezeaku

Anti-nutrient, vitamin and other phytochemical compositions of old and succulent moringa (Moringa oleifera Lam) leaves as influenced by poultry manure application

August 2015

The study was carried out to determine the anti-nutrient, vitamin and other phytochemical compositions of old and succulent leaves of Moringa oleifera plants as influenced by poultry manure application. Three levels of poultry manure, that is, 0, 5 and 10 tonnes ha-1 were used for treatment. Poultry manure application insignificantly (p > 0.05) increased oxalate, phytate and saponin compositions of the leaves. The...

Author(s): Uchenna Mabel Ndubuaku, Nwankwo Vincent Uchenna, Kayode Paul Baiyeri and Juliana Ukonze

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