African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12317

AJB Articles

Diversity analysis of the immunoglobulin M heavy chain gene in Nile tilapia, Oreochromis niloticus (Linnaeus)

July 2015

A full-length cDNA encoding the immunoglobulin (IgM) heavy chain gene of Nile tilapia was successfully cloned using the 5’ and 3’ RACE techniques. The complete cDNA of the Nile tilapia IgM heavy chain gene is 1,921 bp in length and has an open reading frame (ORF) of 1,740 bp, which corresponds to 580 amino acid residues. The deduced amino acid sequence of the Nile tilapia IgM heavy chain includes a typical...

Author(s): Chiraporn Phuyindee, Sasimanas Unajak and Prapansak Srisapoome,

A valid measure to eliminate the influence of polysaccharides and polyphenols in recalcitrant longan (Dimocarpus longan L.) during DNA isolation

July 2015

Large amounts of polysaccharides, polyphenols, tannins, proteins, and other secondary metabolites in recalcitrant longan leaves make it difficult to obtain high quality genomic DNA during extraction. To obtain good quality of nucleic acids from local longan leaves and for its downstream applications, a new protocol was developed. It consists of rapid isolation of stable nuclei, which hinders covalent interactions with...

Author(s): Qingzhi Liang†, Deqiang Gong†, Dingqing Wen and Libao Deng

Hydroponic technology for lily flowers and bulbs production using rainwater and some common nutrient solutions

July 2015

This experiment was carried out to investigate the potential of nutrient film technique (NFT) hydroponic system for flowers and bulbs production of the Asiatic hybrid lily cv. "Blackout" using rainwater and some common nutrient solutions (Hoagland No. 2 Basal Salt Mixture, Murashige and Skoog Basal Salt Mixture and White’s Basal Salt) with rock wool cubes as medium with or without removal of flower buds...

Author(s): Hassan M. Asker

Chemical and quantitive study of hepatotoxins from fresh water cyanobacteria on vertebrate animals

July 2015

Toxicity of the freeze-dried micro-aquatic planktonic cyanobacterium Oscillatoria agardhii, dominating and isolated from Makkah –KSA was studied. Microcystins were detected from freeze-dried cells using high pressure liquid chromatography HPLC. The histopathological examination of mice liver injected on week basis with diluted 1/5 of the lethal dose, (105 mg/ Kg body weight), of toxin extract revealed sever...

Author(s): Al-layl, K. and Mutwally, H.

Biochemical and kinetic characterization of geranylgeraniol 18-hydroxylase (CYP97C27) from Croton stellatopilosus Ohba

July 2015

Geranylgeraniol 18-hydroxylase (EC that exists solely in Croton stellatopilosus Ohba catalyses the last committed step of plaunotol biosynthetic pathways by conversion of geranylgeraniol (GGOH) to plaunotol. This enzyme and its gene are an attractive target for development of plaunotol production and its detailed biochemical properties need to be understood.   Recently, even though the gene...

Author(s): Manee Chanama and Suchart Chanama

Investigation of parietal polysaccharides from Retama raetam roots

July 2015

This study characterizes the cell wall hemicellulose and pectins polymers of Retama raetam. This species develops a particularly important root system and is adapted to arid areas. The cellulose, hemicelluloses and pectins were extracted. The cellulose remains the major component of the wall (27% for young roots and 80% for  adult roots), hemicelluloses (14.3% for young roots and 3.6%  for adult roots) and...


Connecting DNA origami structures using the biotin-streptavidin specific binding

July 2015

This work made use of the strong interaction between biotin and streptavidin to connect designed DNA origami structures. The caDNAno software was used to design a 6 layer 3D origami cross-like structure. Selected DNA strands at the engineered attachment sites on the DNA origami structure were biotinylated. After folding of the origami structures, the biotinylated strands stick out of the attachment sites. Purified...

Author(s): Amoako George, , Ming Zhou Rian Ye, Mensah-Amoah Patrick, Twum Anthony and Sam Frederick

Reproductive performance of dairy cows affected by endometritis, pododermatitis and mastitis

July 2015

The effects of endometritis, pododermatitis and clinical mastitis on the conception rate and calving-conception interval of multiparous and primiparous cows after fixed-time artificial insemination (FTAI) were evaluated. Clinical endometritis was diagnosed by ultrasonography 20-40 days postpartum upon observation of fluid in the uterine lumen. Cows with clinical endometritis were treated intramuscularly with 2 mg/kg...

Author(s): Thaisa Campos Marques, Karen Martins Leão, Moraima Castro Rodrigues, Natalia do Carmo Silva and Rossane Pereira da Silva

Molecular cloning and expression of a novel gene related to legume lectin from Salvia miltiorrhiza Bunge

July 2015

Lectins have been well studied and proved to play important roles in plant defense but information of legume lectins from non-legume plants has been rarely reported. A new legume lectin gene, designated as SmL1, was cloned from Salvia miltiorrhiza Bunge, a famous traditional Chinese medicinal plant. The cDNA of SmL1 was 919 bp in length and contained an 822 bp open reading frame (ORF) encoding a putative lectin...

Author(s): Wenping Hua, Limin Han, and Zhezhi Wang

Molecular screening for erythromycin resistance genes in Streptococcus pyogenes isolated from Iraqi patients with tonsilo-pharyngites

July 2015

Streptococcus pyogenes is the leading cause of uncomplicated bacterial pharyngitis and tonsillitis commonly referred to as strep throat. Erythromycin is administered for patients allergy to penicillin. In this study, 125 throat swab samples were collected from children between 2-12 years old with tonsillo-pharyngitis attended to at the AL-Imammain AL-Kadhimain Medical City-Baghdad-Iraq and Pediatric...

Author(s): Hassan N. Ali, Maysaa A. R. Dhahi and Abdul Kareem H. Abd

Antifungal, acute toxicity and mutagenicity activity of extracts from Datura stramonium, Jacquinia macrocarpa and Krameria erecta on Fusarium verticillioides

July 2015

The effect of Baccharis glutinosa, Jacquinia macrocarpa, and Krameria erecta extracts was investigated on the growth and the spore germination of Fusarium verticillioides (ATCC 52539). Brine shrimp (Artemia salina) was used to evaluate the potential acute toxicity of the fractions obtained from plant extracts. The butanol fraction of J. macrocarpa totally inhibited the radial growth for 144 h and up to 95% after 168 h....

Author(s): M. P. Frías-Escalante, A. Burgos-Hernández, M. Plascencia-Jatomea, M. L. Aldana-Madrid and M. O. Cortez-Rocha

Y-Chromosome short tandem repeat, typing technology, locus information and allele frequency in different population: A review

July 2015

Chromosome Y microsatellites seem to be ideal markers to delineate differences between human populations. They are transmitted in uniparental and they are very sensitive for genetic drift. This review will highlight the importance of the Y- Chromosome as a tool for tracing human evolution and describes some details of Y-chromosomal short tandem repeat (STR) analysis. Among them are: microsatellites, amplification using...

Author(s): Muhanned Abdulhasan Kareem, Ameera Omran Hussein and Imad Hadi Hameed

In vitro embryo rescue and plant regeneration following self-pollination with irradiated pollen in cassava (Manihot esculenta Crantz)

July 2015

Cassava is a highly heterozygous species; hence, current methods used in classical cassava breeding cannot match the urgent need to high yielding varieties. Recently, progress was made through androgenesis and gynogenesis as pathways for raising doubled cassava haploid lines to overcome problems associated with cassava’s inherent reproductive biology, but these efforts were limited (no candidate cassava plantlets...

Author(s): Mary Buttibwa, Robert S. Kawuki,  Arthur K. Tugume, Jacinta Akol, Stephen Magambo, Hellen Apio, Erwin Heberle-Bors, Maria Wedzony, Hernán Ceballos, Clair Hershey and Yona Baguma

Suitability and use of two molecular markers to track race-specific resistance striga gesnerioides in cowpea (Vigna unguiculata (L.) Walp.)

July 2015

The obligate root parasitic weed Striga gesnerioides poses a severe constraint to cowpea productivity in the dry savannahs of West and Central Africa, where cowpea is a major crop. At least seven races of S. gesnerioides have been identified within the cowpea-growing regions of West and Central Africa, based on host differential response and genetic diversity analysis. Molecular markers linked to resistance to...

Author(s): L. O. Omoigui, M. F. Ishiyaku, B. S. Gowda, A.Y. Kamara and M. P. Timko

Adventitious shoots induction and plant regeneration from cotyledons of watermelon (Citrullus lanatus L.)

July 2015

A highly efficient regeneration system is a prerequisite step for successful genetic transformation of watermelon cultivars (Citrullus lanatus L.). The objective of this study was to establish efficient in vitro plant regeneration for three watermelon cultivars. To achieve optimal conditions for adventitious shoot induction, the 5-day-old explants (cotyledon base portion, apical portion and hypocotyl) of three cultivars...

Author(s): Xiaoqiang Zhao, Xiaowei Niu and Min Fan

Physicochemical, antioxidant, and sensorial properties of peach snacks prepared from different cultivars

July 2015

High potentiality of medicinal benefits of peach have increased its demand, however, supply of fresh fruit to meet the demand is challenging as it grows in specific climatic regions and in particular season. Preparation of varieties of processed peach products could be a good option to supply in lean season. Objective of the study was to assess the quality characteristics of peach snacks prepared from 11 different...

Author(s): Kyung-Mi Jung, Suk-Hee Lee, Won-Heum Park, Il-Doo Kim, Sanjeev Kumar Dhungana and Dong-Hyun Shin

Enrichment and isolation of microbial strains degrading bioplastic polyvinyl alcohol and time course study of their degradation potential

July 2015

Polyvinyl alcohol (PVA) degrading bacterial strains were isolated from various environmental sites rich in plastic wastes by using the enrichment culture technique. Among the various isolated strains, the selected potent PVA degrading bacterial strains were tentatively characterized as Bacillus and Pseudomonas sp. The time course of the PVA degradation potential of the characterized strains in growth media containing...

Author(s): Rajashree Patil and U. S. Bagde

Phenotypic characters of yeasts isolated from kpete-kpete, a traditional starter of a Benin opaque sorghum beer

July 2015

Opaque sorghum beers are the most consumed African alcoholic beverages. Tchoukoutou is one of the Benin opaque sorghum beers. Its fermentation process is carried out using a traditional starter called kpete-kpete. The present study characterized and identified the yeasts isolated from kpete-kpete. A total of 24 samples of kpete-kpete were collected from eight different commercial processing sites in Northern Benin. The...

Author(s): Kadjogbe Yves Djegui, Adechola Pierre Polycarpe Kayode, Emile Sedjro Tokpohozin, Emma W. Gachomo, Simeon O. Kotchoni and Joseph Djidjoho Hounhouigan

In vitro microtuberization of Black Zira (Bunium persicum Boiss.)

June 2015

Bunium persicum or Black Zira is one of the endangered species in the land of Persia. The main purpose of this study was to investigate microtuberization of B persicumin in order to use in germplasm storage and commercial production. Seeds of B. persicum were used as explant. Different culture media (MS, ½MS and B5) along with different concentrations of jasmonic acid (JA) (0, 2 and 5) were used individually as...

Author(s): Hossein Mardani, Seyed Mahdi Ziaratnia, Majid Azizi, Han Phyo Aung, Kwame Sarpong Appiah and Yoshiharu Fujii

Effects of aqueous extracts of Mucuna sloanei (Fabaceae) seed on haematological parameters of albino rats (Rattus novergicus)

June 2015

Mucuna sloanei is a legume used as a soup thickener by communities in some parts of Africa countries. The effect of aqueous seed extract of M. sloanei on the haematological profile of normal albino rats was investigated for 28 days using standard methods. The results show no overall dose dependent significant difference (p > 0.05) in the serum levels of the white blood cell count, red blood cell count and its indices...

Author(s): Ejere, Vincent C., Ugwu, Godwin C., Chukwuka, Christian O. and Nnamonu, Emmanuel I.

Investigation of stress tolerance of endoglucanases of the cellulosomes of Clostridium cellulolyticum to ethanol

June 2015

Current energy and environmental challenges are driving the use of cellulosic materials for biofuel production. A major obstacle in this pursuit is poor ethanol tolerance among cellulolytic Clostridium species. The objective of this work was to establish a potential upper boundary of ethanol tolerance for the cellulosome itself. The hydrolytic function of crude cellulosome extracts from Clostridium cellulolyticum on...

Author(s): Ugochukwu Anieto

Fenthion induced toxicity and histopathological changes in gill tissue of freshwater African catfish, Clarias gariepinus (Burchell, 1822)

June 2015

Fenthion is an organophosphate pesticide commonly used in agriculture and public health for the control of insect pests. The present study investigated the toxic effect of fenthion and the histopathological alterations in the gill tissue of African catfish Clarias gariepinus. The 96 h LC50 value of fenthion in C. gariepinus determined by probit analysis was found to be 39.97 mg/L. Fish exposed to different fenthion...

Author(s): Peace Onas Somdare, Christopher Didigwu Nwani, Alfreda O. Nwadinigwe, Jacinta Chinedu Nwani, Gregory Ejike Odo, Ogechi Nnabuchi Ugbor, Juliana A Ukonze and Ada Bridget Chidi Ezeibe

Cellular biomarker responses of bagrid catfish, Chrysichthys nigrodigitatus in a contaminated coastal ecosystem

June 2015

An assessment of the pollution status of Agboyi creek, a water body associated with various anthropogenic activities was carried out in order to determine responses induced in Catfishes, Chrysichthys nigrodigitatus inhabiting it. Cellular biomarkers of stress including the antioxidative stress enzyme, catalase (CAT), lipid peroxidation measured as thiobarbituric acid reactive substance (TBARS), as well as serum enzymes...

Author(s): Ejimadu C. Uzoma, Chukwu L. Obinna and Amaeze H. Nnamdi

Expression of GFSKLYFamide-like neuropeptide in the digestive system of the sea cucumber Holothuria scabra (Echinodermata)

June 2015

Neuropeptides are key mediators of physiological processes in animals and a considerable amount of information has been accumulated on their diversity and functions across phyla. However, progress in echinoderm neurobiology has been much slower than others. The sea cucumber Holothuria scabra is an economically important tropical echinoderm species in which a neuropeptide is yet to be identified. This immunohistochemical...

Author(s): Abayomi Ajayi, and Boonsirm Withyachumnarnkul,

Compound serum and hemin free medium for cultivation of Leishmania tarentolae: A recombinant protein expression system

June 2015

Serum free cultivation of Leishmania is cost-effective and improves large scale production of well-defined parasite material. Moreover, the production of recombinant pharmaceutical proteins requires cultivation of the host in a culture medium free of animal materials, so several culture media for Leishmania tarentolae expression system have been introduced. Some investigations have established the development of a...

Author(s): Jalali Akram, Bandehpour Mojgan, and Kazemi Bahram,

Genetic diversity in Jatropha species from different regions of Brazil based on morphological characters and inters-simple sequence repeat (ISSR) molecular markers

June 2015

Jatropha belonging to Euphorbiaceae family has around 170 species distributed throughout tropical semi-arid regions of Africa and the Americas. Some of its species include Jatropha curcas L. (Physic Nut), Jatropha pohliana Müll.Arg. (Brazilian Purging Nut) and Jatropha gossypiifolia L. (Black Physic Nut). Phenotypic and genetic studies of a population are important for plant improvements, helping in the...

Author(s): Mariana Silva Rosa Pazeto, Sandra Helena Unêda-Trevisoli, Aretha Arcenio Pimentel Corrêa, Viviane Formice Vianna, Daniel Carvalho Leite and Antônio Orlando Di Mauro

Optimization of in vitro multiplication for exotic banana (Musa spp.) in Pakistan

June 2015

The present attempt aimed to optimize micropropagation protocols supplemented with different concentrations and combinations of benzylaminopurine (BAP) (0, 2, 4, 6 mg L-1) and indole acetic acid (IAA) (0.5 and 1.0 mg L-1). Exotic banana (Musa spp) genotypes GCTCV-215 (AAA), ‘Yangambi’ Yangambi Km-5 (AAA) and FHIA-23 (AAAA) were used in research work. Experiments were conducted at Plant Tissue Culture...

Author(s): Mehnaz Qamar, Sadaf Tabassum Qureshi, Imtiaz Ahmed Khan and Saboohi Raza

Growth performance and nutrient quality of three Moringa oleifera accessions grown as potplant under varied manure rates and watering intervals

June 2015

Growth performance and nutrient quality of three Moringa oleifera accessions grown as potherbs under varied manure rates and watering intervals were investigated at the Department of Crop Science, University of Nigeria, Nsukka. The treatments included three accessions of Moringa (Awo-Anaekpa, Idere and Kano), three pig manure rates (0, 10 and 20 t/ha) and three watering intervals (3, 4 and 5 days). These were laid out...

Author(s): K. P. Baiyeri, P. Apeh, G. C. Stevens, O. O. Ndukwe, S. C. Aba and G. T. Otitoju

Provenance variation in growth and genetic potential of Aquilaria malaccensis under nursery condition

June 2015

Aquilaria malaccensis Lam. is commonly known as Agarwood which is distributed in the Indo-malesian genus Aquilaria of family Thymelaeaceae known to produce resin-impregnated heartwood that is fragrant and highly valuable. Agarwood is reputed to be the most expensive wood in the world. Twenty two open pollinated families in A. malaccensis were selected and evaluated for growth attributes and genetic divergence. The...

Author(s): M. B. Noor Mohamed, K. T. Parthiban, R. Ravi and P. Kumar

Application of zein antimicrobial edible film incorporating Zataria multiflora boiss essential oil for preservation of Iranian ultrafiltered Feta cheese

June 2015

Zein based edible film was developed and incorporated with Zataria multiflora boiss essential oil. Mechanical and microbiological characteristics of this biofilms were measured. Increasing concentration of antimicrobial agent in film reduced stretchability, tensile strength and elongation, however increased the thickness and water vapor transmission parameters (P<0.05). Addition of Z. multiflora boiss essential oil,...

Author(s): Sara Ghasemi, Nasrin Haji Seyed Javadi, Mehran Moradi and Kianoush Khosravi-Darani

Minimally processed fruit salad enriched with Lactobacillus acidophilus: Viability of anti-browning compounds in the preservation of color

June 2015

Minimal processing promotes browning of some vegetal tissues due to cell membrane disruption, which results in the release of oxidative enzymes. This study evaluated the efficiency of citric acid, ascorbic acid, sodium metabisulfite and L-cysteine ​​hydrochloride to retard enzymatic browning of minimally processed fruit salad and enriched this product with Lactobacillus acidophilus LA-5. Control treatment was fruit...

Author(s): Eliane Maurício Furtado Martins, Afonso Mota Ramos, Maurilio Lopes Martins, Patrícia Martins de Oliveira and Paulo César Stringheta

Physico-chemical and rheological properties of prato cheese during ripening

June 2015

The influence of storage temperature (6, 12 and 18°C) on texture parameters of Prato cheese were evaluated during 22 days of ripening. A reduction of firmness, gumminess and elasticity was observed; however, cohesiveness and adhesiveness increased. With the increasing temperature, firmness and gumminess reduced, whereas the elasticity, cohesiveness and adhesiveness did not change. It can be concluded that storage...

Author(s): Bruno de Sousa Carvalho⊃, Marco Antônio Pereira da Silva⊃, Jéssica Leal Freitas eSouza⊃, Núbia Ferreira Vieira⊃, Geovana Rocha Plácido⊃, Edmar Soares Nicolau⊃, Moacir Evandro Lage⊃ and Rodrigo Bauduíno Soares Neves⊃

Evaluation of the postharvest quality of Cagaita fruits (Eugenia dysenterica DC.) coated with chitosan and associated with refrigeration

June 2015

Cagaita fruits are subject to seasonality and perishability. This work aims to use scanning electron microscopy (SEM) to evaluate the physicochemical characteristics, texture, color and physical structure of cagaita fruits coated with different chitosan concentrations. The fruits were divided as follows: T0 (uncoated fruits), T1 (fruits coated with 1% (v/v) chitosan), T2 (fruits coated with 2% (v/v) chitosan) and T3...

Author(s): Freitas, BSM, Plácido GR, Cagnin, C, Caliari, M, Silva, RM, Silva, CLC, Cavalcante, MD, Souza, JLF, Célia, JA and Oliveira, KB

Effects of Nigeria Ekete light crude oil on plasma electrolytes, packed cell volume (PCV) and lipids profile in wistar (Rattus norvegicus) rats

June 2015

Hydrocarbon is known to alter blood parameters; some of these blood parameters may affect the activities of certain systems like cardiovascular system. Hydrocarbon gets into man and animal either through ingestion of contaminated food and water, bio-concentration through food chain, occupational exposure or by using hydrocarbon products. Effects of crude oil on plasma electrolytes, packed cell volume (PCV) and plasma...

Author(s): Alaba Olumide Ojo, Jaja S. I., Bamidele Olubayode, Babatunde Lawrence D and Femmymale Taiye F

Therapeutic role of glucogalactan polysaccharide extracted from Agaricus bisporus on trimethyltin chloride induced neuropathy in rats

June 2015

Trimethyltin (TMT) chloride induces limbic system neuro-degeneration, resulting in behavioral alterations including cognitive deficits. The aim of the present study was to investigate the effect of glucogalactan polysaccharide (GA) which purified from the roots of mushroom Agaricus bisporus extract on the Trimethyltin Chloride (TMT) induced neuropathy in rats.  Adult male rats (200 ±10 g) were divided into...

Author(s): Amal I. Hassan, Mona A. M. Ghoneim and Rasha Y. M. Ibrahim

In silico approach to identification of a novel gene responsive to submergence stress in rice

June 2015

Submergence is one of the major constraints to rice production. Bioinformatics approach has been widely used to identify candidate genes on many biological aspects. In the present study, a novel gene involved in submergence stress in rice, Os07g47670 was identified by in silico approach. The amino acid sequence of Os07g47670 is highly homologous to hypoxia-responsive family proteins. No disordered regions are found in...

Author(s): Hewei Du and Min Huang

Construction of a full-length cDNA library and analysis of expressed sequence tags in white jute (Corchorus capsularis L.)

June 2015

White jute (Corchorus capsularis L.) is recognized as an important industrial raw material fibre crop owing to its elite characters. However, little information is known about its molecular basis and genomics. In this study, a complementary DNA library of white jute was constructed and expressed sequence tags (ESTs) were characterized. The titers of original and amplified libraries were 2.32 × 107 and 1.07 ×...

Author(s): Aifen Tao, Xiaozhen Li, Jianmin Qi, Pingping Fang, Lihui Lin, Jiantang Xu, Liwu Zhang, Jianmei Wu and Peiqing Lin

Toxicity assessment of modified Cry1Ac1 proteins and genetically modified insect-resistant Agb0101 rice

June 2015

Insect-resistant Agb0101 rice was recently developed by modifying the cry1ac1 gene by changing codon usage changes relative to the native truncated cry1ac1 gene. To assess the toxicity of genetically modified Agb0101 rice, we conducted bioinfomational comparisons of the amino acid sequences that are not similar to known toxic proteins. Sufficient quantities of mCry1Ac1 protein were produced in Escherichia coli for in...

Author(s): Si Myung Lee, Yun Soo Yeo, Myung Ho Lim, Hyun Suk Cho, Sung Duk Oh, Seon Woo Oh, Soo Yun Park, Soon Jong Kweon, Seok Cheol Suh and Soon Ki Park

Genetic variations between two ecotypes of Egyptian clover by inter-simple sequence repeat (ISSR) techniques

June 2015

The inter-simple sequence repeat (ISSR) markers have been used in order to determine genetic variation and relationship between two clover ecotypes. Ten (10) primers for ISSR were used in this study but only six were successful in generating reproducible and reliable amplicons for different types of the Egyptian clover. The results reveal the polymorphism level by ISSR primers. HB10 ISSR-primer was higher than the rest...

Author(s): Ehab Zayed#, Mervat Sayed and Ahmad Omar,#

Incidence and antimicrobial susceptibility pattern of extended-spectrum-β-lactamase-producing Escherichia coli isolated from retail imported mackerel fish

June 2015

During the past few years, extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and other species of Enterobacteriaceae have become a matter of great concern in human and veterinary medicine. Several studies in recent years documented the prevalence and occurrence of ESBL-producing Enterobacteriaceae in food products such as meat, poultry and raw milk; therefore in this pilot study we examined imported...

Author(s): Nasreldin Elhadi and Khaldoon Alsamman

Screening of yeasts capable of producing cellulase-free xylanase

June 2015

Xylanases have largely been obtained from filamentous fungi and bacteria; few studies have investigated the production of this enzyme by yeasts. The aim of this study was to isolate yeasts from different sources, such as vegetables, cereal grains, fruits, and agro-industrial waste and to obtain yeasts capable of producing celulase-free xylanase. Samples were enriched using yeast malt broth, and yeasts were isolated on...

Author(s): Otero, D. M., Cadaval, C. L., Teixeira, L. M. , Rosa, C. A, Sanzo, A. V. L. and Kalil, S. J

Recovery of active pathogenesis-related enzymes from the apoplast of Musa acuminata infected by Mycosphaerella fijiensis

June 2015

The fungus Mycosphaerella fijiensis causes black Sigatoka (BS) disease, a major pathogen in the banana industry worldwide. Numerous molecular and biochemical studies have been done for the M. fijiensis, Musa acuminata interaction, but this is the first study describing the zymographic behavior of β-1,3-glucanase, chitinase and protease in the apoplast and symplast of healthy, BS-infected but asymptomatic and...

Author(s): Ignacio Islas-Flores, Carmela Alcocer-Alvarez, Yasmín Abril Sánchez-Rodríguez and Blondy Canto-Canché

Carotenoids from Phaffia rhodozyma: Antioxidant activity and stability of extracts

June 2015

The main goal of this work was to establish the stability and antioxidant activity of the extracts obtained through different techniques for recovering carotenoids from Phaffia rhodozyma NRRL-Y 17268. The best conditions for extracting carotenoids through cell rupture with dimethylsulfoxide (DMSO) were found to be a particle size of 0.125 mm submitted to freezing temperature (-18°C) for 48 h (272 µg/g). For...

Author(s): Eliane Pereira Cipolatti, Bruna Araujo Bulsing, Carolina dos Santos Sá, Carlos André Veiga Burkert, Eliana Badiale Furlong and Janaina Fernandes De Medeiros Burkert

Genetic diversity among some productive genotypes of tomato (Lycopersicon esculentum Mill.)

June 2015

This research was done at Vegetable Experimental Farm, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu during spring and summer seasons, 2007-2009. D2-statistics revealed that β-carotene contributed maximally towards the genetic divergence followed by ascorbic acid, total soluble solids, alcohol insoluble solids, pericarp thickness, lycopene content and...

Author(s): Rukhsar Ahmad Dar, J. P. Sharma and Mushtaq Ahmad

Production of friable embryogenic callus and regeneration of Ugandan farmer-preferred cassava genotypes

June 2015

Generation of embryogenic callus is a key step in genetic engineering of many crop species, including cassava. Protocols for generation of friable embryogenic callus (FEC) have been lacking for Ugandan cassava genotypes, thereby delaying their genetic engineering for agronomic and other desirable traits. The objective of this study was to determine conditions suitable for production and regeneration of FEC in the...

Author(s): Hellen B. Apio, Titus Alicai, Yona Baguma, Settumba B. Mukasa, Anton Bua and Nigel Taylor

Dihydrotestostenone increase the gene expression of androgen receptor coregulator FHL2 in human non-transformed epithelial prostatic cells

June 2015

The actions of androgens are mediated through an androgen receptor (AR), and AR activity is modulated by coregulators. The aim of this study was to assess the action of androgens in the expression of AR and the coregulators FHL-2 and SHP-1 in human non-transformed epithelial prostatic cells (HNTEP) treated with androgens. Prostate tissues were obtained from 12 patients between 60 and 77 years of age. HNTEP cells were...

Author(s): Adriane Pozzobon, Diego B Pianta,  Maria Flávia M Ribeiro and Ilma Simoni Brum

Effects of rumen digesta on the physico-chemical properties of soils in Nsukka, Southeastern Nigeria

June 2015

In tropical and subtropical areas, the importance of organic manure in improving soil physico-chemical properties and crop production for food security cannot be overemphasized. A study was conducted to investigate the effects of rumen digesta on the physical and chemical properties of soils in Nsukka, Enugu State, Nigeria. The soil samples collected from Opi, Nsukka were treated with four rates of rumen digesta (viz....

Author(s): Ifeoma Gloria Edeh, Charles Arinzechukwu Igwe and Peter Ikemefuna Ezeaku

Estimation of heritability and genetic gain in height growth in Ceiba pentandra

June 2015

Ceiba pentandra (L.) has been selected for plantation establishment in Ghana under the National Forest Plantation Development Programme of Ghana (NFPDP). This has led to a rise in demand for its seedlings for large scale commercial plantation establishments with seed of C. pentandra being collected from the wild and used to raise seedlings to meet the demands of the programme. The primary purpose of most of these...

Author(s): C. S. Abengmeneng, D.A. Ofori, P. Kumapley, R. Akromah and R. Jamnadass

Evaluation of genetic diversity in barley (Hordeum vulgare L.) from Wollo high land areas using agro-morphological traits and hordein

June 2015

This study aimed to determine the genetic diversity and relationships among barley varieties (Hordeum vulgare L.) growing at Wollo Highland areas by using hordein and agro-morphological traits. Twenty (20) varieties were laid down in randomized complete block design (RCBD) design with three replications; they were planted by irrigation at Wollo University, Dessie Campus from January to May 2014. The genetic analysis...

Author(s): Seid Ebrahim, Eleni Shiferaw and Faris Hailu

Purification and characterization of a new cold active lipase, EnL A from Emericella nidulans NFCCI 3643

June 2015

A mesophilic fungi producing an extracellular cold-active lipase was isolated from the soil samples of palm oil mill effluent dump sites, Pedavegi, West Godavari Dist, A.P. India and was identified as Emericella nidulans. The enzyme was purified by ammonium sulfate fractionation followed by hydrophobic interaction chromatography using phenyl sepharose. The enzyme was 35 fold pure compared to crude with a specific...

Author(s): Suseela Lanka and J. Naveena Lavanya Latha

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