African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12317

AJB Articles

Evaluation of methane production features and kinetics of Bougainvillea spectabilis Willd waste under mesophilic conditions

June 2015

The objective of this study was to investigate and evaluate the biomethane potential (BMP) of Bougainvillea spectabilis Willd waste in Yunnan, China, when subjected to mesophilic anaerobic digestion (AD). Three different categories of plant waste investigated were as follows: the flowers, leaves and stems of B. spectabilia. These three portions were assessed for their BMP in a laboratory-scale batch anaerobic digester...

Author(s): Xiyan Ji, Weidong Lin, Wudi Zhang, Fang Yin, Xingling Zhao, Changmei Wang, Jing Liu, Hong Yang, Yubao Chen and Shiqing Liu

Purification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.)

May 2015

Umbu plants are drought resistant trees which are able to store water and several other substances into its adapted root, named xylopodium. The exsudate from xilopodium is a natural solution rich in salts, sugars and a little concentration of proteins. In this work, we report the purification of a peroxidase (POX) from umbu xilopodium exsudate by direct extraction from polyacrylamide electrophoresis gels. Umbu POX...

Author(s): Marcio dos Santos Teixeira Pinto, Juliana Martins Ribeiro, Francisco Pinheiro de Araújo, Natoniel Franklin de Melo and Kátia Valevski Sales Fernandes

Assessment of four different methods for selecting biosurfactant producing extremely halophilic bacteria

May 2015

Biosurfactants are surface active agents with broad range commercial applications in various industries and have considerable advantages over their chemical counterparts. In this study, drop collapse assay, oil spreading assay, emulsification assay and surface tension measurement were compared for their reliability and ease of use to screen biosurfactant producing six extremely halophilic bacteria isolated from saline...

Author(s): ARIECH Mounira, and GUECHI Abdelhadi

Application of biofilms in the post-harvest conservation of pequi (Caryocar brasiliense Camb.)

May 2015

Pequi (Caryocar Brasiliense Camb.), fruit characteristic of the Brazilian cerrado, has sensory and nutritional characteristics, pleasant flavor and aroma and considerable presence of lipids and fiber, essential for human consumption. The aim of this study was to assess the post-harvest conservation of this fruit by using different sources of biofilms as a means to ensure the maintenance of fruit physical and chemical...

Author(s): Plácido, G. R., Silva, R. M., Cagnin, C., Silva, M. A. P. , Caliari, M. and Furtado, D. C.

Extraction, quantification and degree of polymerization of yacon (Smallanthus sonchifolia) fructans

May 2015

Yacon tubers have been a distinguished alternative of fructans, drawing the attention of researchers and food industries. Since fructans are carbohydrate reserves storage can reduce their contents. Additionally, the type of extraction used can provide a higher yield of fructans. Therefore, it was necessary to study yacon storage and its influence on the extraction and quantification of fructans. Thus, the objective of...

Author(s): Ellem Waleska Nascimento da Fonseca Contado, Estela de Rezende Queiroz, Denise Alvarenga Rocha, Rodrigo Martins Fraguas, Anderson Assaid Simão, Lucimara Nazaré Silva Botelho, Adneia  de Fátima Abreu and Mariana Aparecida Braga Celeste Maria Patto de Abreu

Effects of processing methods on nutritive values of Ekuru from two cultivars of beans (Vigna unguiculata and Vigna angustifoliata)

May 2015

Beans contain substantial amount of protein, dietary fibre, B-vitamins, minerals, and anti-nutrients which limit their utilisation. Processing reduce the level of antinutrients in plant products but little information exist on effects of processing methods on nutrient and antinutrient composition of bean products. This study was undertaken to determine the effects of processing methods on nutrient and antinutrient...

Author(s): Bamigboye Adeola Yewande and Adepoju Oladejo Thomas

Methidathion-induced hematological, biochemical and hepatohistological alterations in rat: Ameliorative effects of selenium and zinc

May 2015

The aim of this study was to determine the effect of a sub-acute 4-week exposure to methidathion (MD) on the hematological system and hepatic integrity of rats. We also assessed whether co-administration of micronutrients such as selenium (Se) and zinc (Zn) prevented MD-induced hepatic damage. Rats were randomly divided into four groups of six each: the first group served as a control which received standard diet, the...

Author(s): Leila Barkat, , Amel Boumendjel, Cherif Abdennour, Mohamed Salah Boulakoud, Abdelfattah El Feki and Mahfoud Messarah

Evaluation of antioxidant and antimutagenic potential of Justicia adhatoda leaves extract

May 2015

In this study, the ethanolic extract of Justicia adhatoda (Acanthaceae) leaves was prepared by successive extraction procedure in increasing polarity order. Moreover, there are no antimutagenic evaluation reports found. In the present study our aim was to determine the antioxidant and antimutagenic potential of different fractions of ethanolic extract of J. adhatoda. Ultra high performance liquid chromatography (UHPLC)...

Author(s): Arvinder Kaur, Davinder Kaur and Saroj Arora

Evaluation of antioxidant capacity and membrane stabilizing potential of stem and root of Cyphospenna adenocaulis (Steud)

May 2015

Comparative analyses of the antioxidant and membrane stabilizing activities of ethanolic extracts of leaf and root of Cyphospenna adenocaulis (Steud) were investigated with a view to further investigate the biological activities of the plant. Both the leaf and root of C. adenocaulis were extracted with 70% ethanol to yield the ethanolic leaf extract (ELE) and ethanolic root extract (ERE) respectively. Phytochemical...

Author(s): Akinwunmi Kemi Feyisayo, Ajala Victor Oluwafemi and Oyedapo Oluboade Oluokun

Fatty acid profile of fish scale of Catla catla

May 2015

Fish scales are useful to ichthyologists for the purposes of classification, identification, age determination and history study. The fatty acid profile of the hexane extracts of the fresh water fish scale of Catla catla were analyzed by gas chromatography. Fish scales were collected from a local fish market, Chidambaram. The fatty acid profile was detected in the scales, with the following fatty acids dominating: 16:0,...

Author(s): K. Prabu, S. Shankarlal and E. Natarajan

A new vehicle for herbicide application using crude glycerin, a by-product of biodiesel production

May 2015

The supply of glycerin derived from the pre-purification of biodiesel has increased considerably in Brazil, making it necessary to identify economic and environmentally friendly applications for this by-product. This work aimed to develop oil-in-water (O/W) emulsions using crude glycerin treated with H3PO4 for use as a vehicle for the application the herbicide Togar. The work was conducted in the laboratory of...

Author(s): Rejanne Lima Arruda, José Iran Cardoso da Silva, Evilanna Lima Arruda, Marciane Cristina Dotto, Renato de Almeida Sarmento and Eduardo Andrea Lemus Erasmo

In silico characterization and evolution studies of alcohol dehydrogenase gene from Phoenix dactylifera Deglet Nour

May 2015

The aim of our study was to isolate the alcohol dehydrogenase (ADH) mRNA from Phoenix dactifera, and examine the molecular evolutionary history of this nuclear gene with others ADH genes from palms and other plants species. The DnADH gene has been isolated in silico by BLAST2GO from a cDNA library of date palm cv Deglet Nour. The prediction of candidate’s mRNA and protein for ADH gene from khalas were performed in...

Author(s): Imen Rekik, Amine Elleuch, Noureddine Drira and Foued Cheour

Responses of potatoes plants inoculated with arbuscular mycorrhizal fungi and litter in greenhouse

May 2015

A pot experiment was set to examine the impact of the foliar litter (Hardwickia binata and Azadirachta indica) and an arbuscular mycorrhizal (AM) fungus on the development of two varieties of potato plants (Aida, Atlas). Three litter doses (0, 25 and 50 g) were applied to the pots after bedding plantlets. The plants were inoculated with AM, Glomus aggregatum. Mycorrhizal colonization, shoot dry weight, size and number...

Author(s): Bathie Sarr, Fatimata Ndiaye, Malick Ndiaye, Mame Arame Fall Ndiaye and Tahir Abdoulaye Diop

Induced polygenic variability using combination treatment of gamma rays and ethyl methane sulphonate in blackgram (Vigna mungo (L.) Hepper)

May 2015

Induced mutation in plant improvement has been proven to be one of the alternative ways to generate new sources of genetic variation in blackgram. In this study, dry seeds of VBN 4 blackgram were treated with combination treatment of both gamma rays (400, 500 and 600 Gy) and ethyl methane sulphonate (EMS) (50, 60 and 70 mM) to study the polygenic characters in M2 generation. The mean values for plant height, number of...

Author(s): K. S. Usharani and C. R. Ananda Kumar

Growth of Chlorella vulgaris and Nannochloris oculata in effluents of Tilapia farming for the production of fatty acids with potential in biofuels

May 2015

The use of microalgae in wastewater treatment and its biotechnological exploitation for the production of biofuels is a potential environmental application. Some species of microalgae are notable due to their lipid composition and fatty acid profile suitable for biofuel production. During the present study, a factorial 23 experimental design was conducted, which assessed three factors: i) two species of microalgae...

Author(s): Yesica I. Ferrer-Álvarez, Luis A. Ortega-Clemente, Ignacio A. Pérez-Legaspi, Martha P. Hernández-Vergara, Paula N. Robledo-Narváez, Elvira Ríos-Leal and Héctor M. Poggi-Varaldo

Microbiological evaluation and shelf life of seed flour mixes used for infant feeding in rural northern Nigeria

May 2015

This study investigates microbial load and shelf life of locally processed cereal-legume-oil seed flour mixes used for infant feeding in rural northern Nigeria.  Free fatty acid (FFA), water activity (aw), pH, total viable count (TVC) and presence of yeast, coliform bacteria and mold were the parameters determined. Hungry rice or Digitaria exilis, benne seed or Sesamum indicum and soybean or Glycine max seeds...

Author(s): Ibeanu, V. N., Ene-Obong, H. N., Peter-Ogba, G. U. and Onyechi, U. A.

Design of experiment approach for the process optimization of ultrasonic-assisted extraction of polysaccharide from mulberry leaves by response surface methodology

May 2015

Mulberry is considered as food-medicine herb, with specific nutritional and medicinal values. In this study, response surface methodology (RSM) was employed to optimize the ultrasonic-assisted extraction of total polysaccharide from mulberry using Box-Behnken design (BBD). Based on single factor experiments, a three level, three variable central composite designs were carried out to establish a quadratic regression...

Author(s): Zhao Mengdi#, Zhou Hong#, Wang Heng , Weiguo Zhao, , Hyong-Jun Kim and Liu Li

Separation and purification of chlorogenic acid from tobacco by-products by polyamide and silicagel column chromatography

May 2015

Methods for separation of chlorogenic acid (CA) from tobacco by-products were established. The liquid chromatography tandem mass spectrometry (LC-MS) method for identification and analysis of chlorogenic acid from tobacco materials has been developed. CA was isolated by polyamide and further purified by silicagel column chromatography. Results reveal that polyamide is suitable for separation of CA from tobacco extract....

Author(s): Wenwen Zhao, Yuru Chen , Shaofeng Li, Kewei Dai, Yan Chen and Shijie Yang

Physical-chemical characteristics and antioxidant potential of seed and pulp of Ximenia americana L. from the semiarid region of Brazil

May 2015

Ximenia americana popularly known as wild plum grow wildly in Brazilian semiarid region and its fruit were harvested in two maturity stages and evaluated for quality. The experimental design was completely randomized with three treatments (immature, mature pulp and seeds), and treatment effect was evaluated for pulp fraction (composed of both mesocarp and exocarp or peel) in maturation stages:...

Author(s): José Darcio Abrantes Sarmento, Patrícia Lígia Dantas de Morais, Francisco Israel de Souza and Maria Raquel Alcântara de Miranda

Acute toxicity and behavioral changes of Caspian kutum (Rutilus frisii Kutum Kamensky, 1991) and Caspian roach (Rutilus rutilus caspicus Jakowlew, 1870) exposed to the fungicide hinosan

May 2015

Pesticides are used in agriculture to control pest and protect human health and animals. Excessive use of pesticides caused risk for human health and threated non-target organisms, polluted water, soil and air. Hinosan is a component of organophosphate pesticide which is used as a fungicide in agricultural fields. In the study, lethal concentration (LC50) of Hinosan was calculated for Rutilus frisii kutum and Rutilus...

Author(s): Saeid Shahbazi Naserabad, Alireza Mirvaghefi, Mohammad Hasan Gerami and Hamed Ghafari Farsani

Cellulolytic activity of gut extract of subterranean termite, Odontotermes obesus Rambur: A pretreatment tool for conversion of lignocellulosic biomass to fermentable sugar for biorefinery industry

May 2015

Lignocellulosic biomass is a chief and cheap raw material for bioethanol production. However, pretreatment is a critical and most expensive step in lignocellulosic biomass to biofuel conversion. Biological pretreatments offer an alternative for lignocellulosic biomass conversion using enzyme hydrolysis. Termites are well known for the ability to digest lignocelluloses, using it as a sole food source. To effectively...

Author(s): Natchiappan Senthilkumar, Sourimuthu Murugesan and Devaraj Suresh babu

Physicochemical and microbiological meat quality, growth performance and carcass characteristics of feedlot-finished cull Santa Inês ewes and Moxotó goats¹

May 2015

This study aimed at assessing the physicochemical and microbiological quality of feedlot-finished, culled Santa Inês ewes and Moxotó goats, and compares the intake, weight gain and carcass characteristics. Three Santa Inês ewes and Moxotó goats with similar ages were confined in double and/or individual pens and then euthanized after 40 days of confinement. The average intake, daily weight...

Author(s): Beatriz Severino da Silva, Geovana Rocha Plácido, Elis Aparecido Bento, Wellingthon da Silva Guimarães Júnnyor and Marco Antônio Pereira da Silva

Molecular identification of tsetse fly (Diptera: Glossinidae) species based on mitochondrial DNA (COII and CytB) sequences

May 2015

Tsetse fly (Diptera: Glossinidae) anti-vector measures are reliant upon accurate identification of species and their subpopulations. Two species were studied, Glossina palpalis palpalis and Glossina morsitans submorsitans using two mitochondrial DNA: cytochrome oxidase subunit II (COII) and cytochrome b (CytB). Sequencing data were used to perform phylogenetic analysis of the two reared species together with other...

Author(s): Christopher U. Orji, Ignatius O. Onyeocha, Steven S. Shaida, Peter M. Dede, Pam D. Luka, Bitrus Yakubu and Elijah E. Ella

Determination of long-term effects of consecutive effective fresh chicken manure with solarization and verticillium wilt (Verticillium dahlia Klebb) on weed and its control in egg plant

May 2015

The aim of the study was to determine the weed density and the most economical way of weed control in eggplant (Solanum melongena) fields contaminated with Verticillium dahliae (Kleb) after the application of fresh chicken manure and solarization in the second year as the same crop was grown. The effect of solarization on weed and the labor need in weed control continued in a diminishing way in the consecutive...

Author(s): Cumali ÖZASLAN, Ismail ÇİMEN, M. Zeki KIZMAZ, Vedat PIRINÇ and Abdurrahman KARA

Pleurotus pulmonarius cultivation on amended palm press fibre waste

May 2015

In the last few decades, rapid global demand for edible oils has resulted in a significant increase in the land area of oil crop cultivation. In the process of extraction of palm oil from oil palm fruit, biomass materials such as palm pressed fibre (PPF) are generated as waste products. This research was undertaken to evaluate the use of palm pressed fibres as the substrates for the cultivation of Pleurotus pulmonarius...

Author(s): Mojeed Olaide Liasu, Adeyemi Ojutalayo Adeeyo, Emmanuel Oluwafunminiyi Olaosun and Rebecca Oyedoyin Oyedokun

Evaluation of soil microbial communities as influenced by crude oil pollution

May 2015

Impact of petroleum pollution in a vulnerable Niger Delta ecosystem was investigated to  assess interactions in a first-generation phytoremediation site of a crude oil freshly-spilled agricultural soil. Community-level approach for assessing patterns of sole carbon-source utilization by mixed microbial samples was employed to differentiate spatial and temporal changes in the soil microbial communities. Genetic...

Author(s): Eucharia Oluchi Nwaichi and Magdalena Frac,

Characterization of the dominant microorganisms responsible for the fermentation of dehulled maize grains into nsiho in Ghana

May 2015

Nsiho (white kenkey) is a type of kenkey, a sour stiff dumpling, produced from fermented maize meal in Ghana. The dominant microorganisms responsible for the fermentation of nsiho were characterized by analysing samples from four traditional production sites at Anum in the Eastern Region of Ghana. During 48 h of steeping dehulled maize grains, the pH values decreased from 6.05 to 5.93 to 3.59 to 3.55, whilst titratable...

Author(s): Food Research Institute,Council for Scientific and Industrial Research,P.O. Box M.,Accra, Ghana. Department of Nutrition and Food Science,University of Ghana, Legon, Accra, Ghana.

The nutritional quality of Spirulina platensis of Tamenrasset, Algeria

May 2015

Spirulina platensis, a blue green microalga, has been used since ancient times as a source of food because of its high protein content (65%) and nutritional value. Lipids isolated from S. platensis have been shown to contain high levels of polyunsaturated fatty acids, including linolenic acid which is a precursor of arachidonic acid; this cyanobacteria contains, also, several kinds of sterols. The aim of this study is...


Inclusion of sweet sorghum flour in bread formulations

May 2015

Sweet sorghum (Sorghum bicolor L. Moench) has been studied as an additional source of raw material for production or partial replacement of foods due to its high fiber concentration. Its consumption is associated with the prevention of some diseases and nutritional benefits. The aim of this study was to evaluate the partial replacement of wheat flour by sweet sorghum flour in bread formulations in order to characterize...

Author(s): Veronica Freitas Pires Araujo, Wellingthon da Silva Guimaraes Junnyor, Marco Antonio Pereira da Silva, Geovana Rocha Placido, Marcio Caliari Caliari, Maria Siqueira de Lima and Nubia Ferreira Vieira

Critical evaluation of proteomic protocols for passion fruit (Passiflora edulis Sims) leaves, a crop with juice market benefits

May 2015

Passion fruit grows practically all over Brazilian territory; its production is largely destined to juice industry and expanding to overseas markets. The suitability of four protein extraction protocols for plant proteome was investigated to determine the best choice for studies concerning passion fruit leaf proteins. Trichloroacetic acid (TCA)/acetone extraction; isoelectric focusing (IEF) buffer extraction; phenol...

Author(s): Viviane A. Perdizio, Olga L. T. Machado, Jucelia S. Araujo, Antonia E. A. Oliveira, Leandro R. Monteiro, Monique N. Costa, Andre T. Ferreira, Jonas Perales and Tania Jacinto

Analysis of alkaloid phytochemical compounds in the ethanolic extract of Datura stramonium and evaluation of antimicrobial activity

May 2015

The aim of this study was to assess the compounds of alkaloids extracts from the leaves of Datura stramonium, which can be the basis for the synthesis of new antibiotics. In this study, the alkaloid compounds of D. stramonium have been evaluated. The chemical compositions of the leaves of ethanolic extract of D. stramonium were investigated using gas chromatography-mass specroscopy. Gc-MS analysis of alkaloid leaves...

Author(s): Huda Jasim Altameme, Imad Hadi Hameed and Muhanned Abdulhasan Kareem

Effects of manganese, 2,5-xylidine, veratryl alcohol and tween 80 on the production of ligninolytic enzymes by Ceriporiopsis subvermispora

May 2015

The effects of adding manganese, 2,5-xylidine, veratryl alcohol and Tween 80 in a culture medium used for the production of ligninolytic enzymes by polyurethane foam-immobilized Ceriporiopsis subvermispora were studied. While 11 ppm Mn2+ promoted the highest maximum activity of manganese peroxidase (108.0 ± 43.3 U/L, in the 6th day of cultivation), the medium without manganese led to the highest maximum...

Author(s): Robson A. Faria, Adriane M. F. Milagres and Walter Carvalho

A review on marine based nanoparticles and their potential applications

May 2015

The increasing demands on nanoparticles have wide pertinent in almost all the fields. Marine ecosystem has variety of living resources, which includes prokaryotes like microorganism to eukaryotic organism like higher plants and animals. The present review dealt with the application of marine organisms in nanotechnology. Our discussion mainly focused on what the marine organisms are involved in and what type of...

Author(s): Chinnappan Ravinder Singh, Kandasamy Kathiresan and Sekar Anandhan

The use of multiplexed simple sequence repeat (SSR) markers for analysis of genetic diversity in African rice genotypes

May 2015

Rice is an emerging food and cash crop in Eastern Africa. Thousands of germplasm accessions have been introduced from major rice breeding centers, such as the International Rice Research Institute (IRRI), and Africa Rice but the genetic variability among the introduced rice germplasm is unknown. Knowledge on genetic diversity would be useful in designing measures for comprehensive breeding and conservation. To address...

Author(s): Bonny M. Oloka, , Jimmy Lamo, Patrick Rubaihayo, Paul Gibson and Juan Vorster

Molecular diversity study of black cumin (Nigella sativa L.) from Ethiopia as revealed by inter simple sequence repeat (ISSR) markers

May 2015

Nigella sativa L. (commonly known as black cumin) belonging to family Rannunculaceae is an important medicinal plant with worldwide distribution. In Ethiopia, N. sativa occurs in all regions and agro-ecologies at different altitudinal ranges. This plant has a lot of importance in Ethiopia. However, there is no information available on molecular genetic diversity of this crop in respect to Ethiopia. Therefore, the aim of...

Author(s): Birhanu Kapital, Tileye Feyissa, Yohannes Petros and Said Mohammed

Inter simple sequence repeat (ISSR) analysis of Ethiopian white lupine (Lupinus albus L.)

May 2015

White lupine (Lupinus albus L.) collected from two zones (West Gojjam and Awi) of Amhara region and one zone (Metekel) of Benishangul - Gumuz regional state of Ethiopia were studied using inter simple sequence repeat (ISSR) markers in an attempt to assess the genetic diversity. Four ISSR primers of which three were dinucleotide repeats and one, a penta nucleotide repeat amplified a total of 39 clear and reproducible...

Author(s): Abdie Oumer, Petros Yohannes, Tesfaye Kassahun, Teshome Abel and Bekele Endashaw

Genotype x environment interaction and stability analysis for yield and yield related traits of Kabuli-type Chickpea (Cicer arietinum L.) in Ethiopia

May 2015

Chickpea is the major pulse crop cultivated in Ethiopia. However, its production is constrained due to genotype instability and environmental variability. This research was carried out to examine the magnitude of environmental effect on yield of chickpea genotypes and to investigate the stability and adaptability of genotypes under different agro-ecologies. Seventeen (17) genotypes were evaluated in randomized complete...

Author(s): Getachew Tilahun Firew Mekbib, Asnake Fikre and Million Eshete

Effects of the humic acid extracted from vermicompost on the germination and initial growth of Brachiaria brizantha cv. MG5

May 2015

The biological effects of humic substances on vegetables depend on the source of extraction and the concentration used, on the vegetable species and on the age of the plant. This study aimed to evaluate the effect of different humic acid (HA) doses extracted from vermicompost on the germination and initial growth of Brachiaria brizantha cv. MG5. To that end, germination tests were conducted in germination agents, as...

Author(s): Mariá Moraes Amorim, Henrique Duarte Vieira, Isabela Moraes Amorim, Leonardo Barros Dobbss, Bruno Borges Deminicis and Priscilla Brites Xavier

Effect of alkaline treatment on the sulfate content and quality of semi-refined carrageenan prepared from seaweed Kappaphycus alvarezii Doty (Doty) farmed in Indian waters

May 2015

Seaweed Kappaphycus alvarezii previously known as Eucheuma cottonii is one of the best sources of kappa carrageenan and is cultivated in Philippines, Indonesia, Malaysia and other countries including India. In the present study, semi-refined carrageenan (SRC) was prepared from K. alvarezii with different concentrations of KOH (6, 12, 18 and 24%) at 80 ± 2°C for 2 h; its sulfate contents were...

Author(s): J. Moses, R. Anandhakumar and M. Shanmugam

Modification of chitin as substrates for chitinase

May 2015

Enzymes are able to bind to their substrates specifically at the active site. The proximity and orientation of the substrates strongly increase the likelihood that productive E–S complexes will arise. Treated chitin (powder or flake) is more efficient than crystalline chitin. This is because the latter is less active due to its insolubility. The structure of treated chitin is opened; this facilitates its...

Author(s): Nuniek  Herdyastuti, Sari Edi Cahyaningrum, Mizan Tamimi and Adi Wirawan

Isolation of microalgae species from arid environments and evaluation of their potentials for biodiesel production

May 2015

Twenty-five (25) strains of microalgae were isolated and screened for growth, lipid accumulation and biodiesel production from arid environments of North East Nigeria. Isolates that produced biomass concentration (≥ 1.50 g L-1 cell dry weight), accumulated high concentrations of lipids (≥ 18% of the cell biomass) and could be purified on agar plates were selected for further studies. Four strains morphologically...

Author(s): Innocent Okonkwo Ogbonna and James Chukwuma Ogbonna

Genetic diversity analysis of mustard (Brassica spp.) germplasm using molecular marker for selection of short duration genotypes

April 2015

Molecular characterization of 16 mustard (Brassica spp.) genotypes by using 12 RAPD markers revealed that three primers GLA-11, OPB-04 and OPD-02 showed good technical resolution and sufficient variations among different genotypes. A total of 40 RAPD bands were scored of which 38 (94.87%) polymorphic amplification products were obtained. Besides, the primer OPD-02 amplified maximum number of polymorphic bands (100.00%)...

Author(s): Md. Shahin Iqbal, Islam Hamim, Md. Shamiul Haque and Ujjal Kumar Nath

Isolation and identification of native lower fungi for polyunsaturated fatty acid (PUFA) production in Thailand, and the effect of carbon and nitrogen sources on growth and production

April 2015

This research focuses on isolation and identification of polyunsaturated fatty acid (PUFA) producing fungi from natural sources of Thailand, followed by experimental designs for carbon and nitrogen utilizations of the potential strains. The results show that 8 and 16 isolates of lower fungi from freshwater and wet-fallen leaves, respectively, could produce PUFAs. Among these isolates, the NR06 that was isolated from...

Author(s): Supavadee Deelai, Satinee Suetrong, Somchit Damrianant, Panida Unagul and Niramol Sakkayawong

Molecular genetic diversity study of Lepidium sativum population from Ethiopia as revealed by inter simple sequence repeat (ISSR) markers

April 2015

Lepidium sativum L. (family Brassicaceae), is an underutilized medicinal plant with worldwide distribution. In Ethiopia, L. sativum occurs in all regions and agro-ecologies at different altitudinal ranges. The study was conducted to assess the genetic diversity of L. sativum population from Ethiopia using inter simple sequence repeat (ISSR) marker. Molecular data generated from ISSR bands recorded was used for computing...

Author(s): Said Mohammed and Kassahun Tesfaye

Effects of selected culture media on mycellial growth of oyster mushroom (Pleurotus ostreatus)

April 2015

The effect of selected culture media on mycelial growth of Pleurotus ostreatus was studied on six different culture media: three synthetic [malt extact agar (MEA), potato dextrose agar (PDA) and Saubroud dextrose agar (SDA)] and three formulated from locally available agricultural wastes [corncob (CCEA), palm waste (PWEA) and cassava peels (CPEA)]. The tissue culture of the mushroom was done on MEA which was used as the...

Author(s): Chuku, S. B., Nwachukwu, E. O. and Stanley, H. O.

The population structure of wild sorghum species in agro-ecological zones of Western Kenya

April 2015

There is need to understand the genetic structure of wild sorghums that grow alongside cultivated traditional sorghum varieties in order to assess the potential effect of crop genes in wild populations. In this study, 175 wild sorghum samples were collected from 13 agroecological zones (AEZs) from three counties in Western Kenya and genotyped using microsattelite markers. Crop alleles were observed in wild sorghum...

Author(s): Titus O. Magomere, Eliud K. Ngugi, Silas D. Obukosia , Eunice Mutitu and Solomon I. Shibairo

Insights on predominant edible bamboo shoot proteins

April 2015

Juvenile bamboo shoots have become a US$ 18 billion industry and have lured interest worldwide for its nutritive value and health enhancing properties; making it a suitable candidate for food security. Quantitative analysis revealed that juvenile bamboo shoots are a good source of proteins. However, there is no qualitative analysis describing unique proteins present in the edible bamboo shoots. In order to provide the...

Author(s): Sayanika Devi Waikhom, Louis Bengyella,, Pranab Roy and Narayan Chandra Talukdar

Activity of the fungus Pleurotus ostreatus and of its proteases on Panagrellus sp. larvae

April 2015

Biological control has been shown to be one of the possible biotechnological applications of fungi and their proteases. The objective of this study was to evaluate the nematicidal activity of the fungus Pleurotus ostreatus and its proteases on Panagrellus sp. larvae. Proteolytic activity of P. ostreatus (PLO 06) was measured and characterized at different pHs, temperatures and in the presence of a inhibitor (PMSF)....

Author(s): Hugo Leonardo André Genier, Filippe Elias de Freitas Soares, José Humberto de Queiroz, Angélica de Souza Gouveia, Jackson Victor Araújoa, Fabio Ribeiro Braga, Iara Rebouças Pinheiro and Maria Catarina Megumi Kasuya

Enhanced recombinant human blood coagulation factor VIII expression in the milk of goats using replication-defective adenovirus

April 2015

High-level expression of recombinant human blood coagulation factor in milk of farm animals at a large scale provides a powerful tool for protein production. However, a bottleneck in recent protein synthesis technologies is the high cost of current transgenic livestock system. Here, we report a simple, rapid, and low-cost protein production method based on a replication-defective adenoviral vector system. The...

Author(s): Zengsheng Han, Lin Wang, Kun Li, Jian Li, Hongwei Zhao, Zhiwei Liu, Wenguang Cao and Qingwang Li,

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