African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12267

AJB Articles

Real time PCR mediated determination of the spontaneous occurrence of Sorghum bicolor alleles in wild sorghum populations

February 2015

  The study evaluates the utility of Real Time PCR (RT-PCR) in quantitative and qualitative analysis of alleles in sorghum populations and the spontaneous occurrence of Sorghum bicolor alleles in wild populations of sorghum. Leaf and seed material from wild sorghum accesions were sampled in Homabay, Siaya and Busia counties to represent Western Kenya sorghum producing regions. A second sampling was done on S2...

Author(s):   Titus O. Magomere, Eliud K. Ngugi, Solomon I. Shibairo, Eunice Mutitu and Silas D. Obukosia

Polyamines and WOX genes in the recalcitrance to plant conversion of somatic embryos of Habanero pepper (Capsicum chinense Jacq.)

February 2015

  In order to determine the role of polyamines in the formation and development of the somatic embryos of Capsicum chinense, the effect of different concentrations (0, 0.01, 0.1, and 1.0 mM) of Putrescine, Spermidine and Spermine on the efficiency and morphology of the embryos was evaluated. The results show that none of the three polyamines evaluated had a significant effect on the number of embryos formed,...

Author(s):   Raúl Enrique Valle-Gough, Susana Alejandra Avilés-Viñas, Stephanie López-Erosa, Adriana Canto-Flick, Eunice Gómez-Uc, Luis Alfonso Sáenz-Carbonell, Neftalí Ochoa-Alejo and Nancy Santana-Buzzy

Copper-induced changes in growth and antioxidative mechanisms of tea plant (Camellia sinensis (L.) O. Kuntze)

February 2015

  Tea is the oldest, most popular, non-alcoholic caffeine containing beverage in the world. Tea plants are prone to the attack of many diseases which can be controlled by the treatment of pesticides. These pesticides contain heavy metal which prolonged accumulation can lead to the damage of crop yield both in quantity and quality. In the present study, we observed the effects of high concentration of Cu stress...

Author(s):   Sarmishta Dey, P. B. Mazumder and S. B. Paul

A two-stage decentralised system combining high rate activated sludge (HRAS) with alternating charcoal filters (ACF) for treating small community sewage to reusable standards for agriculture

February 2015

  Water scarcity increasingly drives wastewater recovery. Campaigns towards re-use of wastewater are not very common in Africa among other factors, due to a lack of efficient and cost-effective technology to treat wastewater to re-usable standards. In this study, two treatment systems, a high rate activated sludge (HRAS) system and alternating charcoal filters (ACF) are combined and used to treat wastewater to...

Author(s):   Irene Nansubuga, Francis Meerburg, Noble Banadda, Korneel Rabaey and Willy Verstraete

Characterization of indole acetic acid endophyte producers in authoctonus Lemna gibba plants from Xochimilco Lake

February 2015

Xochimilco’s lacustrine zone is a network of channels that, along with the chinampas, conform a unique ecosystem which has served as source of aquatic resources. Duckweeds are small free-floating monocotyledon aquatic plants classified as macrophytes that serve as nutrient pumps and reduce eutrophication effects. Recently, there are number of new studies related to the aquatic plant-microbial interaction focused...

Author(s): Orlando Ortega-Acosta, Aida Verónica Rodriguez-Tovar, Eugenia López-López and Angélica Rodríguez-Dorantes

Effects of gamma radiation on enzymatic production of lignolytic complex by filamentous fungi

February 2015

This work aimed to study the effect of gamma radiation on the production of enzymes by filamentous fungi present in the seawater used for thermoelectric Termope S / A, in the vicinity of Port of Suape, Pernambuco. The isolated microorganisms were screened for their ability to produce enzymes. Subsequently, the fungi were irradiated at 3 doses (500, 1000 and 1500 Gy), using 4 inoculation techniques: lyophilisation,...

Author(s): Nelânia Maria de Queiroz Baptista, Evelyne Gomes Solidônio, Flávia Virgínia Ferreira de Arruda, Erik Jonne Vieira de Melo, José Robson Neves Cavalcanti Filho, Maira Judith de Azevedo Callou, Rita de Cássia Mendonça de Miranda, Waldeciro Colaço and Norma Buarque de Gusmão

Production of α-amylase by solid state fermentation by Rhizopus oryzae

February 2015

A thermotolerant strain of Rhizopus oryzae was grown in three agro-industrial by-products: brewers’ rice, corn grits and wheat bran. Different substrates, cultivation time, moisture content, additional nitrogen sources, pH and temperature of incubation were evaluated aiming to optimize growing conditions. The highest enzymatic activity was observed after 24 h of cultivation using wheat bran as substrate with the...

Author(s): Osania Emerenciano Ferreira, Nayara Abrão Montijo, Eduardo da Silva Martins and Marcia Justino Rossini Mutton

Acute oral toxicity and cytotoxicological evaluation of the ethanol extract of Samanea tubulosa pods in Swiss mice

February 2015

Acute toxicity and cytotoxicity of ethanol extract of Samanea tubulosa (EESt) pods were evaluated in Swiss mice. Acute toxicity studies were conducted based on  OECD guidelines 420, where the limit test dose was 5000 mg/kg. Observation was made and recorded systemically for 1, 2, 4 and 24 h after the administration of dose for skin changes, morbidity, aggression and sensitivity of the behavior of the animals. For...

Author(s): Paulo Alex Bezerra Sales, Lucas Antonio Duarte Nicolau, Jamylla Mirck Guerra de Oliveira, Marina Rebeca Soares Carneiro de Sousa, Mariana Helena Chaves, Fernando Aécio de Amorim Carvalho, Enoque Pereira Costa Sobrinho Junior and Amilton Paulo Raposo Costa

Evaluating the antioxidant and anticancer property of Ficus carica fruits

February 2015

The present study was designed to investigate the antioxidant and anticancer properties of Ficus carica (FC). The dried coarse powder of F. carica fruits was exhaustively extracted with ethanol and the resulting crude ethanolic extract (FCE) was assayed for antioxidant and anticancer activities, since both are inter-related. The antioxidant activity of FCE was determined by reductive potential in reducing ferric ions...

Author(s): Jasmine R, Manikandan K and Karthikeyan

Structural, physical, functional and nutraceutical changes of freeze-dried fruit

February 2015

This review examines the structural, physical, functional and nutraceutical changes of lyophilized fruits. Collapse, porosity, color, glass transition temperature, rehydration capacity, ability to retain water, volatile compounds, phenolic compounds, ascorbic acid, and beta-carotene, were defined, and the causes of changes in these parameters, during freeze-drying, were analyzed. Advantages and limitations of the...

Author(s): Liliana Serna-Cock, Diana Patricia Vargas-Muñoz and Alfredo Ayala Aponte

Examination of genetic diversity in common bean (Phaseolus vulgaris L.) using random amplified polymorphic DNA (RAPD) markers

February 2015

To study the pattern of genetic diversity in 45 genotypes of common bean, 19 RAPD primers were used. Of 253 bands produced, 236 bands (94.22%) were polymorphic in which maximum number (20 polymorphic bands) were observed in the profiles of the primer OPB-07. Highest PIC value (0.79) was observed for the primers OPG-14 and OPE-1, whereas lowest PIC value (0.34) was recorded for the primer OPA-11 with an average PIC value...

Author(s): Asifa Bukhari, M. A. Bhat, Mushtaq Ahmad and Noorul Saleem

Ethanol production using hemicellulosic hydrolyzate and sugarcane juice with yeasts that converts pentoses and hexoses

February 2015

The use of vegetable biomass as substrate for ethanol production could reduce the existing usage of fossil fuels, thereby minimizing negative environmental impacts. Due to mechanical harvesting of sugarcane, the amount of pointer and straw has increased in sugarcane fields, becoming inputs of great energy potential. This study aimed to analyze the use of hemicellulosic hydrolyzate produced by sugarcane pointers and...

Author(s): Juliana Pelegrini Roviero, Priscila Vaz de Arruda, Maria das Graças de Almeida Felipe and Márcia Justino Rossini Mutton

Feedstuffs potential of harvest by-products from two oleaginous cucurbits

February 2015

Among the generated crop residues and by-products in tropical agriculture, those of cucurbits represent great opportunities for animal nutrition. Nutritive profile of harvest by-products (dried leaves, fermented fruits, non fermented fruits and seeds shells) of two oleaginous cucurbits (Citrullus lanatus and Lagenaria siceraria) were investigated in order to explore their potential use as feedstuffs. The moisture, ash,...

Author(s): A. I. Touré, A. L. Loukou, K.K. Koffi, I. Mouaragadja, G. L. Bohoua, B. Mbatchi and B. I. A. Zoro

Influence of rural processing methods and postharvest storage treatments on quality characteristics of kola nut (acuminata Schott & Endl.)

February 2015

Quality changes of kola nut (Cola acuminata) as affected by processing methods and short-term storage environments were investigated. The experiment was conducted at the Teaching and Research Laboratory of the Department of Crop Science, University of Nigeria Nsukka, Nigeria. The treatments comprised of: three different colour plastic buckets – red, green, white and three inner linings - Newbouldia laevis leaves,...

Author(s): Eze, S. C., Ameh, G. I., Ugwuoke, K. I., Onyeke, C. C. and Ozioko, J. O.

Antitrypanosomal activity of Khaya senegalensis and Anogeissus leiocarpus stem bark on Trypanosoma brucei brucei infected rats

February 2015

Trypanosoma brucei brucei, a haemo-protozoan parasite causes African Animal Trypanosomiasis (AAT). Khaya senegalensis (KS) and Anogeissus leiocarpus (AL) are medicinal plants used either individually or in combination by local farmers in Northern Nigeria in the treatment of many diseases including trypanosomiasis. There is however, no information on the efficacy of the plants used in combination. In this study, the...

Author(s): Awobode, Henrietta O., Fagbemi, Folasade T. and Afolayan, Funmilayo I. D.

Allelopathic potential of some biocontrol agents for the control of fungal rot of yellow yam (Dioscorea cayenensis Lam)

February 2015

The adverse effect of synthetic pesticides on human health and the natural ecosystem necessitate the need to explore natural mechanisms of disease control in plants. This study evaluated the allelopathic potential of five biocontrol agents: Trichoderma longibrachiatum, Trichoderma asperellum, Bacillus subtilis, Bacillus cereus and Pseudomonas fluorescens in the control of six fungal pathogens associated with tuber rot...

Author(s): Dania, V. O., Fadina, O. O., Ayodele, M. and Lava Kumar, P.

Growth of mycotal species on the eggs of Cyprinus carpio in limnologically and trophically different water bodies

February 2015

The study investigated the growth of hydromycoflora on the eggs of common carp (Cyprinus carpio L.) in five water bodies of different eutrophication levels. Thirty-three (33) species of mycotal organisms, including 28 species belonging to Peronosporomycota, 2 to anamorphic fungi and 1 each to Ascomycota, Blastocladiomycota and Zygomycota were identified on the eggs. The water from River Biala and Pond Fosa, which are...

Author(s): Bazyli Czeczuga, Adrianna Semeniuk- Grell and Ewa Czeczuga- Semeniuk

Exploitation of molecular genetics in microbial degradation and decolorization of industrial waste water effluent

February 2015

Activated sludge samples were used to analyse microbial community structures from the anoxic and aerobic zones of a labora­tory-scale modified Ludzack-Ettinger system. Fluorescent in situ hybridization and denaturing gradient gel electrophoresis were applied for analysis. With the help of DNA specific fluorochrome DAPI, approximately 75 to 80% of total cells were detected, hybridised with a specific eubacterial...

Author(s): Shah M. P. 

Influence of temperature on the anaerobic stabilization of organic solid residues

February 2015

This study was aimed at determining the effect of temperature on the stabilization of organic solid waste conjugated with sewage sludge in anaerobic batch reactors (ABR), and to estimate the efficiency of the process in producing biogas and methane. The substrate contained 36.2 g L-1 of total solids comprising residues from fruits and vegetables enriched with anaerobic sludge from sanitary sewage (4:1, w/w). The...

Author(s): Jorge Marcell Coelho Menezes, Valderi Duarte Leite, Aldre Jorge Morais Barros, Wilton Silva Lopes, José Tavares de Sousa and Andrezza Raphaella Costa Campos

Understanding the efficacy of influent waste water on microbial community structure of activated sludge process

February 2015

The assembling of microbial consortia in wastewater treatment facilities is a significance of environmental conditions. In the present research work, activated sludge from different wastewater treatment plants (WWTPs) were exploited at a molecular level to determine the influence of the complexity of the influent composition on the species structure and the diversity of bacterial consortia. The community fingerprints...

Author(s): Shah M. P. 

Fungal infection in freshwater fishes of Andhra Pradesh, India

February 2015

A total of 17 isolates of fungi were isolated from diseased fishes which belong to five species namely Saprolegnia diclina, Saprolegnia ferax, Saprolegnia hypogyana, Saprolegnia parasitica and Achlya americana. All these fungi were isolated from six different species of fresh water fishes viz. Channa stratius, Channa punctatus, Clarias batrachus, Labeo rohita, Heteropneuste fossilis and Mystus cavasius. The parasitic...

Author(s): S. A. Mastan

Use of molecular biology techniques in the detection of fraud meat in the Egyptian market

February 2015

Food safety and quality are major concerns and any case of food adulteration has a great impact on public opinion. Identification of animal species used in commercial meat products is important with respect to economic and sanitary issues. The aim of this research was to detect ruminant and equine species in minced meat and Egyptian sausage using polymerase chain reaction-restriction fragment length polymorphism...

Author(s): Dalia Zahran and Sanaa Hagag

Forensic analysis of mitochondrial DNA hypervariable region HVII (encompassing nucleotide positions 37 to 340) and HVIII (encompassing nucleotide positions 438-574) and evaluation of the importance of these variable positions for forensic genetic purposes

February 2015

The first objective of this study was the detection of mitochondrial hypervariable HVII and HVIII regions. Secondly, the study evaluates the importance of these positions for forensic genetic purposes and establishes the degree of variation characteristic of a fragment. Blood samples were collected from 270 healthy unrelated male living in Middle and South of Iraq. FTA® Technology was utilized to extract DNA. A...

Author(s): Imad Hadi Hameed, Mohammed Abdullah Jebor and Muhanned Abdulhasan Kareem

Detection of new variant “Off-ladder” at the D12S391, D19S433 and D1S1656 loci and tri-allelic pattern at the D16S539 locus in a 21 locus autosomal short tandem repeat database of 400 Iraqi Individuals

February 2015

The objectives of the study included the detection of genetic variation of 21 autosomal short tandem repeats (STRs) loci from random unrelated individuals in the middle and South Arab people of Iraq, the Forensic efficiency parameters of the autosomal 21 genetic loci using power plex21® kit and to evaluate the importance of these loci for forensic genetic purposes and the possibility to use the new kit in routine...

Author(s): Mohammed Abdullah Jebor, Imad Hadi Hameed and Muhanned Abdulhasan Kareem  

Development of microsatellite markers for use in breeding catfish, Rhamdia sp.

February 2015

Microsatellites markers for catfish, Rhamdia sp. were developed using Next Generation. A shotgun paired-end library was prepared according to standard protocol of Illumina Nextera DNA Library Kit with dual indexing, paired-end reads of 100 base pairs and grouped with other species. From a single race, five million readings obtained were analyzed with the program PAL_FINDER_v0.02.03. perl script was used to extract...

Author(s): Marília Danyelle Nunes Rodrigues, Carla Giovane Ávila Moreira, Harold Julian Perez Gutierrez, Diones Bender Almeida, Danilo Streit Jr. and Heden Luiz Marques Moreira

Cultivation and bromatological analysis of the medicinal mushroom Ganoderma lucidum (Curt.: Fr.) P. Karst cultivated in agricultural waste

February 2015

The objective of the study was to evaluate the production of two strains of Ganoderma lucidum on agricultural waste and carry out bromatological analyses of the basidiomata obtained from the cultivation. The experiment was carried out at the Mushroom Module at the School of Agronomic Sciences of the São Paulo State University (FCA/UNESP - Botucatu, SP, Brazil) and two strains were used (GLM-09/01 and GLM-10/02)...

Author(s): Cristiane Suely Melo de Carvalho, Ceci Sales-Campos, Leise Pessoa de Carvalho, Marli Teixeira de Almeida Minhoni, André Luiz Merthan Saad, Guilherme Polidoro Alquati And Meire Cristina Nogueira de Andrade

Use of multivariate analysis to evaluate the effect of sucrose on in vitro cassava conservation

February 2015

The aim of this work was to evaluate the effect of sucrose to reduce the in vitro growth of cassava plants using multivariate statistical tests. Cassava conservation has a relevant role as an auxiliary strategy for preservation and genetic breeding. Micro-cuttings of cassava accessions BGM 264, BGM 265, BGM 1037 and BGM 1282 from the Active Germplasm Bank of the Embrapa Cassava and Fruits were tested with five different...

Author(s): Lívia de Jesus Vieira, José Raniere Ferreira de Santana, Ádila Melo Vidal, Alfredo Augusto Cunha Alves, Antônio da Silva Souza, Carlos Alberto Ledo da Silva and e Fernanda Vidigal Duarte Souza

Inducible secretion of phytate-degrading enzymes from bacteria associated with the medical plant Rosa damascena cv. Taifi using rice bran

February 2015

More than 320 bacteria were isolated from the soil (Rhizosphere, endophyte, flowers and leaves) of Rosa damascena cv. Taifi and screened for phytase activity. Phytase activity was checked for 24 isolates in Bacillus broth media supplemented with and without rice bran. Twelve (12) isolates were found with detectable phytase activities. Among them, four selective bacterial strains were active (BAFA.Taifi94, BAFA.Taifi103,...

Author(s): Abd-ElAziem Farouk, Abdulelah  Banaja, N. Thoufeek Ahamed, Othman AlZahrani and Salih Bazaid

Sero-prevalence of infectious bursal disease in backyard chickens around Mekelle, Northern Ethiopia

February 2015

A cross sectional study was conducted from January to June 2012 to determine the sero-prevalence of Infectious bursal disease (IBD) in chicken reared under backyard poultry production systems around Mekelle town, Tigray regional state. During the study period blood samples were collected from 384 unvaccinated backyard chickens from three different areas. A commercial indirect enzyme linked immuno sorbent assay (ELISA)...

Author(s): Sinidu Zegeye, Yisehak Tsegaye, Haftay Abreha and Nesibu Awol

Analysis of polymorphisms in the mitochondrial ND5 gene in Pantaneira and Creole breeds of sheep

February 2015

The aim of this study was to assess genetic variation between a population of Pantaneira sheep in the Brazilian state of Mato Grosso do Sul, and Creole sheep from the south of the country by molecular analysis of the ND5 gene in mitochondrial DNA. The analysis revealed the presence of 16 haplotypes with all Pantaneira sheep grouped together carrying a single haplotype, and there was no grouping with any of the...

Author(s): Joyce Azambuja de Oliveira, Andrea Alves do Egito, Bruno do Amaral Crispim, Fernando Miranda de Vargas Junior, Leonardo de Oliveira Seno and Alexéia Barufatti Grisolia

Ascorbic acid, β-carotene, total phenolic compound and microbiological quality of organic and conventional citrus and strawberry grown in Egypt

January 2015

A two year study at Alexandria University compared ascorbic acid, β-carotene, total phenolic compound, nitrite content and microbiological quality of orange and strawberry fruits grown under organic and conventional management techniques to see if producers concerns are valid. Organically grown oranges and strawberries had lower titratable acidity (TA); whereas, there was no significant difference in total soluble...

Author(s): Hoda A. Khalil and Shimaa M. Hassan

Influence of the reuse of progesterone implants in a fixed-time artificial insemination protocol on the conception rates of lactating cows during two different seasons

January 2015

This study aimed to evaluate the influence of reusing progesterone intravaginal implants using a fixed-time artificial insemination (FTAI) protocol on the conception rates of 593 primiparous and multiparous lactating cows during two different seasons. The cows were divided into two categories: multiparous and primiparous. The experiment was conducted during seasons with high and mild temperatures. To...

Author(s): Thaisa Campos Marques, Karen Martins Leão, Natalia do Carmo Silva, Moraima Castro Rodrigues, Rossane Pereira da Silva and Patricia Antonio

Genetic diversity of Bambara groundnut (Vigna subterranea (L.) verdc.) landraces in Kenya using microsatellite markers

January 2015

The existence of genetic diversity in germplasm collections is crucial for cultivar development. Genetic relationships among 105 Bambara groundnuts (Vigna subterranea (L) Verdc.) accessions from Kenya were evaluated using 12 microsatellite markers. The Bambara landraces were collected from farmers in the western region and the National Genebank of Kenya. A total number of 24 alleles were revealed with a mean of 2...

Author(s): Forrester O. Odongo, Maurice E.Oyoo, Victor Wasike, James O. Owuoche, Laura Karanja and Paul Korir

Activity of Cinnamomum zeylanicum essential oil and ethanolic extract against extended-spectrum β-lactamase-producing bacteria

January 2015

The antibacterial effects of Cinnamon (Cinnamomum zeylanicum) essential oil and ethanolic extract against extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Pseudomonas aeruginosa strains were studied in the present study. The essential oil and ethanolic fraction of C. zeylanicum showed significant activities against all tested microorganisms and minimal...

Author(s): Hend Abdulhmeed Hamedo

Paclobutrazol biodegradation in unsaturated soil in the Semi-Arid Northeast of Brazil

January 2015

Paclobutrazol (PBZ) is a plant growth regulator, increasing flowe ring and yield that is widely used in mango cultivation in the semi-arid northeastern Brazil. PBZ remains active in the soil for several years. However, it can severely affect the growth and development of subsequent crops, mainly by reducing vegetative vigor. The aim of this study was to investigate PBZ biodegradation in four samples of soil: P-G (with...

Author(s): Fernanda L. Vaz, Thaís A. Gomes, Caio C. A. Rocha, Maria Aparecida Mouco and Ester R. Gouveia

Polymorphism of Pfatpase6 in Côte d’Ivoire: Detection of a four new point mutations

January 2015

Over the past decade, the number of malaria cases has dropped by more than half in many malaria-endemic countries. However, recent parasite resistance to artemisinin undermines that progress. Artemisinin-based combination therapy (ACTs) is recommended for the treatment of Plasmodium falciparum malaria. Among the potential genes that are associated to resistance of P. falciparum to artemisinin include PfATPase6 gene that...

Author(s): Brice Kouakou Bla, Jonhson Noël David Trébissou, Houphouet Félix Yapi, William Yavo, Francis Adou Yapo and Joseph Allico Djaman,

Amylolytic strains of Lactobacillus plantarum isolated from barley

January 2015

Two α-amylase-producing strains of Lactobacillus plantarum were isolated from South African barley. The extracellular α-amylase activity produced by strain A.S1.2 coincided with cell growth, while strain B.S1.6 produced α-amylase mainly during stationary growth. Cell wall α-amylases in both strains were approximately five times higher than recorded for extracellular α-amylases. Both strains...

Author(s): Hattingh, M. , Alexander, A., Meijering, I., Van Reenen, C. A. and Dicks, L. M. T.

Biodegradation of low density polyethylene (LDPE) by a new biosurfactant-producing thermophilic Streptomyces coelicoflavus NBRC 15399T

January 2015

In the present study, we tested 83 actinomycete isolates for low density polyethylene (LDPE) degradation in the laboratory, and these isolates were previously isolated for biosurfactant production from the oil contaminated soil sample near Naval Dockyard in Visakhapatnam. Among them, 20 were positive using 2,3,5-triphenyl tetrazolium chloride (TTC) as a viability indicator and able to grow in a mineral salts medium...

Author(s): Midhun Kumar Duddu, Kalyani Lakshmi Tripura, Girijasankar Guntuku and Dandu Sree Divya

Effects of drying temperature on viability of macaw palm (Acrocomia aculeata) zygotic embryos

January 2015

In response to a growing interest in improving seedling production of oilseed species (like macaw palm), a fruit drying protocol for facilitating seed extraction was proposed. This enabled the production of macaw palm seedlings, but the temperature most suitable for seed extraction without losing its physiological quality is unknown. The goal of this study was to evaluate the effects of different drying temperatures on...

Author(s): Aurélio Rubio Neto, Fabiano Guimarães Silva, Juliana de Fátima Sales, Larissa Leandro Pires, Bethânia Silva Morais de Freitas and Apolyana Lorrayne Souza

Genomic composition factors affect codon usage in porcine genome

January 2015

The objective of the study was to determine the codon usage bias in the porcine genome and decipher its determinants. To investigate the underlying mechanisms of codon bias, the coding sequence (CDS) from the swine reference sequence (ssc10.2) was extracted using Biomart. An in house built Perl script was used to derive various genomic traits and codon indices. Analysis was done using R statistical package, and...

Author(s): Khobondo, J. O., Okeno, T. O., and Kahi, A. K.

Sodicity tolerant polyembryonic mango root stock plants: A putative role of endophytic bacteria

January 2015

The importance of extracellular enzyme producing endophytic bacteria and their ability to elicit ‘induced systemic tolerance’ against abiotic stress (sodic soil) has been documented. However, the performance of these microbes under various abiotic stresses, especially saline-sodic conditions is less understood and formed the focus of this investigation. The diversity of endophytic bacteria associated with...

Author(s): Kannan, R., Damodaran, T. and Umamaheswari, S.

Phytosynthesis of eco-friendly silver nanoparticles and biological applications –A novel concept in nanobiotechnology

January 2015

Nano-biotechnology is an undoubtedly future generation technology which offers potential applications in multidisciplinary areas of science and technology. In the present day’s production, stabilization and utilization of nanoparticles is the eliminatory division in modern science receiving immense attention of scientists engaged in different fields of research. A number of metal nanoparticles have been engineered...

Author(s): M. Chandrasekhara Reddy, K. Sri Rama Murthy, A. Srilakshmi, K.R.S.Sambasiva Rao and T. Pullaiah

Molecular characterization of the plum collection [Prunus domestica (L.) Borkh] of the Pedagogical and Technological University of Colombia

January 2015

Eight Random Amplified Microsatellite markers (RAMs) were used to characterize the genetic diversity found in 14 Prunus materials belonging to the deciduous collection of the Pedagogical and Technological University of Colombia. A total of 121 bands were generated: they range from nine for the GT primer to 26 for the ACA primer, and have molecular weights between 100 and 2050 Kb. At a Nei-Li similarity level of 0.75,...

Author(s): Ana Cruz Morillo C., Yacenia Morillo C., Elberth Hernando P. and Ivan Adiel Avila M.

Genetic assessment of some phenotypic variants of rice (Oryza spp.) for some quantative characters under the Gangatic plains of West Bengal

January 2015

Twenty two (22) recombinant inbreed lines (RIL’s) derived from interspecific cross derivative of Oryza sativa and Oryza rufipogon along with two local check varieties MTU 7029 and Ranjit were evaluated in randomized block design (RBD) with two replications at two different environments (1st at Regional Research Station, New Alluvial Zone (NAZ), Bidhan Chandra Krishi Viswavidyalaya, Sub-Centre, Chakdah, Nadia, West...

Author(s): B. K. Senapati and Awaneet Kumar

‘Do-sono’ passion fruit: Ecogeographical prospecting and phenotypic dispersion in transition areas between the caatinga and cerrado of Brazil

January 2015

This study performed eco-geographical prospecting on five populations of wild ‘de-sono’ passion fruit plants in the rural zones of five towns from two distinct agroecological units in the state of Bahia. Ripe fruit from plants from these populations that had fallen to the ground were physicochemically characterized and had their phenotypic variability dispersion among the populations estimated via uni- and...

Author(s): Douglas de Almeida Pereira,  Ronan Xavier Correa  and Antonio Carlos de Oliveira

Antimicrobial susceptibility assessment of compound from Aspergillus fumigatus

January 2015

Ethyl acetate extract of the culture filtrate of Aspergillus fumigatus on chromatographic analysis has led to the isolation of the compound, AF-1 which exhibited a significant in vitro antimicrobial activity against the tested pathogenic microorganism. The structure of the isolated compound, AF-1 was identified as 5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one from its IR, UV, EI-MS, 13C-NMR, 1H-NMR, HMBC and HMQC data....

Author(s): Zakia Sultana Sathi, Md. Mustafezur Rahman, Muhammad Arifur Rahman, Md. AL- Faruk and Mohammad Abdur Rashid,

Physiological properties of facultative and obligate alkalophilic Bacillus sp. strains isolated from Saudi Arabia

January 2015

Isolation and identification of new alkalophilic Bacillus strains have increasing interest due to their possessing valuable and commercially interesting enzymes. To date, several researchers have studied the identification and characterization of alkalophilic Bacillus strains based on the phenotypic characterization as phylogenetic analysis of 16SrRNA. In the present study, six obligate and facultatively alkalophilic...

Author(s): Assaeedi Abdulrahman

Phytochemical screening and antimicrobial activities of the leaf extract of Entandrophragama angolense

January 2015

The phytochemical screening and antimicrobial activities of the leaf extract of Entandrophragma angolense were investigated using the agar well diffusion method. The methanolic extracts at crude level were shown in vitro to inhibit Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Streptococcus cremoris with diameter zones of inhibition ranging from 6 to 14 mm. Minimum inhibitory concentration was 3.13...

Author(s): G. A. Shittu and E. S. Akor

Enhancement of biomass production and nutrition utilization by strain Lactobacillus acidophilus DGK derived from serial subculturing in an aerobic environment

January 2015

Successive subculturing under aerobic conditions for 14 days could be used to improve characteristics of a parent strain with a growth defect. Identification of the derived strain was based on the API 50 CHL test kit and partial sequencing of the hsp60 gene. Results show that an aerotolerant strain, Lactobacillus acidophilus DGK, was created with improved nutrition utilization....

Author(s): Chin-Fa Hwang, Chien-Ku Lin, Shin-Yi Yan, Ren-Han Chang and Hau-Yang Tsen

Induction and characterization of pathogenesis-related proteins in roots of cocoyam (Xanthosoma sagittifolium [L] Schott) infected with Pythium myriotylum

January 2015

Although Pythium myriotylum is a very destructive root pathogen of cocoyam, the host defense response in this plant-pathogen interaction has not been fully studied. Four cocoyam germplasm accessions were inoculated with P. myriotylum, and their induced defense responses were characterized. The induction and spatio-temporal accumulation of chitinase and b-1,3-glucanase were determined by enzymatic activity assays of...

Author(s): Leopold M. Nyochembeng and Caula A. Beyl

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