African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

AJB Articles

Enhancement of biomass production and nutrition utilization by strain Lactobacillus acidophilus DGK derived from serial subculturing in an aerobic environment

January 2015

Successive subculturing under aerobic conditions for 14 days could be used to improve characteristics of a parent strain with a growth defect. Identification of the derived strain was based on the API 50 CHL test kit and partial sequencing of the hsp60 gene. Results show that an aerotolerant strain, Lactobacillus acidophilus DGK, was created with improved nutrition utilization....

Author(s): Chin-Fa Hwang, Chien-Ku Lin, Shin-Yi Yan, Ren-Han Chang and Hau-Yang Tsen

Induction and characterization of pathogenesis-related proteins in roots of cocoyam (Xanthosoma sagittifolium [L] Schott) infected with Pythium myriotylum

January 2015

Although Pythium myriotylum is a very destructive root pathogen of cocoyam, the host defense response in this plant-pathogen interaction has not been fully studied. Four cocoyam germplasm accessions were inoculated with P. myriotylum, and their induced defense responses were characterized. The induction and spatio-temporal accumulation of chitinase and b-1,3-glucanase were determined by enzymatic activity assays of...

Author(s): Leopold M. Nyochembeng and Caula A. Beyl

Effects of Moringa oleifera Lam. aqueous leaf extracts on follicle stimulating hormone and serum cholesterol in Wistar rats

January 2015

The study evaluated the effect of Moringa oleifera aqueous leaf extracts on follicle stimulating hormone and serum cholesterol in Wistar rats. Thirty six (36) mature Wistar rats (20 male and 16 female rats) were used. The male rats were grouped into four groups with five animals each, while the female animals were grouped into four made up of four animals per group, on the basis of their body weights. Graded doses (1, 5...

Author(s): Nwamarah, Joy Ugo, Otitoju, Olawale and Otitoju, Grace T. O.

Effect of different hydrolysis methods on starch degradation

January 2015

Hydrolysis of cassava starch was carried out using different processing routes namely: malt extract; acid; combinations of α-amylase and amyloglucosidase; combinations of acid, α-amylase and amyloglucosidase and combinations of malt extract, α-amylase and amyloglucosidase. The results of hydrolysis of all the five routes shows a wide degree of variance in their susceptibility to acid/enzyme hydrolysis...

Author(s): F. A. Aderibigbe and L. A. Adejumo

Quality characterization of Niger seed oil (Guizotia Abyssinica Cass.) produced in Amhara Regional State, Ethiopia

January 2015

In the present investigation, an attempt has been made to find out cholesterol and total free fatty acid content in Niger seed oil which is the most available edible oil in Ethiopia. Acid value, peroxide value, saponification value and cholesterol content were determined. The analysis performed using Liebermann-Burchard method revealed no level of cholesterol content. Unlike the peroxide value, acid value and...

Author(s): Dimberu G. Atinafu, Shamshad K. B. and Solomon Libsu

Simple sequence repeat (SSR) markers are effective for identifying pear cultivars and selections

January 2015

The present study characterized and identified pear cultivars growing in the southern region of Minas Gerais State, Brazil, using microsatellite markers. Nineteen (19) pear cultivars were collected from two sites of Southern Minas Gerais State: Ribeirão Vermelho and Lavras. DNA was extracted from newly formed leaves and amplified using 21 simple sequence repeat (SSR) markers (NH001c, NH002b, NH005b, NH007b,...

Author(s): Juliana Stracieri⊃, Hélida Mara Magalhães⊃, Luciane Vilela Resende⊃ and Luis Carlos Cirilo Carvalho⊃

Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers

January 2015

Malaysia is a genetically diverse hotspot for wild relatives of Mangifera and other Anacardiaceae members. The genus Bouea is interesting in that it has small sweet-sour edible fruits. This genus consists of two species, namely Kundang (Bouea macrophylla) and Remia (B. oppositifolia) which are of economic value. In this study, we highlighted the molecular evidences using inter simple sequence repeats (ISSR) markers to...

Author(s): Mohd Norfaizal Ghazalli, Muhamad Fahmi Yunus and Abdul Latiff Mohammad

Genetic diversity of Tunisian melon (Cucumis melo. L) landraces and their relationships with introduced varieties as assessed by simple-sequence repeat (SSR) markers

January 2015

The genetic diversity and the relationships among Tunisian melon landraces and introduced varieties belonging to different varietal groups were estimated using six simple-sequence repeat (SSR) markers. All loci were polymorphic and provided a total of 56 alleles, with an average of 9.33 alleles per locus. The allelic frequencies differed according to accessions, and particular alleles were found within several...

Author(s): Rym TRIMECH, Makram AFIF and Mohamed BOUSSAID

Identification of genes that have undergone adaptive evolution in cassava (Manihot esculenta) and that may confer resistance to cassava brown streak disease

January 2015

Cassava (Manihot esculenta) is a vital food security crop and staple in Africa, yet cassava brown streak disease (CBSD) and cassava mosaic disease result in substantial yield losses. The aim of this study was to identify genes that have undergone positive selection during adaptive evolution, from CBSD resistant, tolerant and susceptible M. esculenta varieties and inter-specific hybrids, as well as a wild cassava...

Author(s): M. Ferguson, T. M. Koga, D. A. Johnson, K. A. Koga, G. N. Hirsch, L. A. Becerra Lopez-Lavalle, and W. Messier

Micropropagation of Phytolacca dodecandra L'Herit (Endod var. E-44)

January 2015

Phytolacca dodecandra L’Heit (Endod var. E-44) is a medicinal plant of high saponin content used as a snail control agent in schitosomiasis. The aim of this study was to develop a micrpropagation protocol for P. dodecandra. Nodal and shoot tip explants were sterilized and cultured on MS medium supplemented with different types of concentrations and combinations of auxin and cytokinin for shoot induction,...

Author(s): Jiregna Daksa, Balcha Abera and Tewodros Taddese

Forage crops as substrate for animal feed and ethanol production in Thailand

January 2015

Five forage crops, namely ruzi (Brachiaria ruziziensis), purple guinea (Panicum maximum), atratum (Paspalum atratum), plicatulum (Paspalum plicatulum), and rhodes grass (Chloris gayana), were experimented for their possibility of ethanol and animal feed utilization. All tested forage crops were harvested 45 and 75 days after being planted. The results indicate the effect of harvesting time on their composition,...

Author(s): Pilanee Vaithanomsat, Phanawan Thongsuntie, Phatthara Khomkamon and Darunee Srichana

Evaluation of extracts of Coleus species for antibacterial activity

January 2015

Antibacterial activity of 50%-methanol, water and absolute methanol extracts of Coleus species (whole plant) was evaluated using agar, and macro-broth dilution methods. The test microorganisms were strains of Staphylococcus aureus, Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa. The yields achieved with extracts were 24% for absolute methanol, 22% for the water and 39% for the 50% methanol extracts,...

Author(s): Jacqueline Ebob Tarh, Josephine Ifeanyinwa Okafor and Christian Ukwuoma Iroegbu

Milk clotting and proteolytic activity of enzyme preparation from Pediococcus acidilactici SH for dairy products

January 2015

Some microorganisms have the ability to produce enzymes that could clot milk and used as a substitute for calf rennet. Strains of lactic acid bacteria (LAB) could produce proteolytic enzymes that may have the potential to be used as a source of milk clotting enzyme (MCE). In the present study, LAB isolated from shrimp paste identified as Pediococcus acidilactici SH, was evaluated for milk clotting activity (MCA),...

Author(s): Mohamed Muftah Imdakim, Zaiton Hassan, Mohamed Mustafa Aween, brahim Mustafa Elshaafi and Belal Jamal Muhialdin

Chemical and physicochemical characterization of the seed oil from ‘Gefner’ atemoya

January 2015

Atemoya is a hybrid fruit derived from the crossing of sugar-apple (Annona squamosa L.), with cherimoya (Annona cherimola Mill.). The objective of this study was to compare two extraction methodologies for the seed oil from ‘Gefner’ atemoya, for yield, chemical and physicochemical characteristics, in order to use the oil as biofuel. The physical extraction was shown to be more economically viable than the...

Author(s): Luciana Soares da Cruz, Angelita Duarte Corrêa, Valquíria Alves Bastos, Pedro Castro Neto and Luciana de Matos Alves Pinto

Physicochemical and sensory profile of yogurt added with passion fruit peel flour

January 2015

Yogurt is a type of fermented milk with high nutritional value. The addition of passion fruit peel flour to yogurt may help the retention of water due to the presence of starch, in addition to being a source of nutrients and fiber. Thus, the aim of this study was to evaluate the physicochemical parameters, color and sensory profile of yogurt added with passion fruit peel flour. Four yogurt formulations with...

Author(s): Vieira, N. F., Silva, M. A. P., Martins, Y. A. A., Souza, D. G., Lima, M. S., Plácido, G. R. and Caliari, M.

Histologic and ultrastructural observations on the thyroid gland of the White Fulani (Zebu) cattle in Northern Nigeria

January 2015

The histology and ultrastructure of thyroid gland of White Fulani (Zebu) cattle were examined during the postnatal developmental period to show if variations in morphology exist with age and in relation to tropical climate. Eight prepubertal and ten pubertal cattle of abattoir origin were used to carry out this study. Histologically, the thyroid consisted of well-developed capsule, interlobular connective tissue,...

Author(s): Casmir Onwuaso Igbokwe and Daniel Nwagbo Ezeasor

Characterization of microbial degradation of oxytetracycline in river water and sediment using reversed phase high performance liquid chromatography

January 2015

The fate of oxytetracycline (OTC) in river water and sediment and control experiments was investigated. A high-performance liquid chromatography (HPLC) separation and identification method was used to separate, identify and quantify OTC and its major degradation products. Minimum degradation of OTC was observed in control experiments. Non microbial degradation observed up to day 26 contributed slightly above 20% of the...

Author(s): Dzomba, P., Kugara, J. and Zaranyika, M. F.

Comparison of fatty acid profile of wild and farm reared freshwater prawn Macrobrachium rosenbergii (De Man) brooders for broodstock diet formulation

January 2015

Fatty acid profiles of midgut gland (MG), ovary and eggs of Macrobrachium rosenbergii of wild and farm reared brooders indicate a significant variation of their components during the sexual maturation and spawning. In both groups, major fatty acids found in the chosen tissues were 14:0, 16:0, 18:0, 18:1n-9, 18:2n-6, 18:3n-3, 20:4n-6, 20:5n-3 and 22:6n-3. Of these, saturated fatty acids dominate over the mono-unsaturated...

Author(s): Paramaraj Balamurugan, Pitchaimuthu Mariappan and Chellam Balasundaram

Utilization of sweet potato starches and flours as composites with wheat flours in the preparation of confectioneries

January 2015

Freshly harvested roots of sweet potato variety TIS 87/0087 were processed into flour and starch using standard methods. Blends of Wheat and sweet potato composite flour was developed in the ratios 80:20, 70:30, 60:40 and 50:50. Also, blends of wheat and sweet potato starch were developed in the ratios 80:20, 70:30, 60:40 and 50:50. Whole sweet potato flour and starch were also included where 100% wheat flour was used...

Author(s): Hussein Adinoyi Etudaiye, Emmanuel Oti, Chinyere Aniedu and Majekodunmi Rachael Omodamiro

Screening model for indolocarbazole-producing microorganisms by molecular probe combined with high performance liquid chromatography (HPLC) method

January 2015

Indolocarbazole (ICZ) alkaloid constitutes a group of natural products which manifested powerful biological activity, especially anticancer. However, it was laborious to find ICZs-yield organism when anticancer activity was adopted as selection target. The specificity of 6 primer pairs was detected and the results revealed that primer rebBN1/rebBC1 possessed preferable specificity because indolocarbazole-producers DNA...

Author(s): Zhenping Shi, Jiaojiao Dong, Wenhan Lin, Aigang Yang, Jiuming Zhang and Li Tian,

Identification and characterization of putative conserved IAM-hydrolases in developing rice grains

January 2015

Indole-3-acetamide (IAM) pathway was proposed to produce indole-3-acetic acid (IAA). This pathway was proven to be functional in many species of bacteria and expected to operate in plants based on the identification of IAM and IAM-hydrolase (AMI) activity in certain plant species. Available putative AMI sequences from a wide array of monocot and dicot plants were identified and the phylogenetic tree was constructed and...

Author(s): Yousef M. Abu-Zaitoon

The effects of storage conditions on the viability of enteropathogenics bacteria in biobanking of human stools: Cases of Yersinia enterocolitica, Salmonella enterica Typhimurium and Vibrio cholerae O: 1

January 2015

Long-terms recoverability of enteropathogens is necessary for future epidemiological studies to screen stool samples when conditions do not permit immediate processing. The aim of this study was to determine the viability and the recoverability of three enteropathogens bacteria (Yersinia enterocolitica, Vibrio cholerae O: 1 and Salmonella enterica Typhimurium) artificially contaminated at 107 CFU/ml in human stool...

Author(s): SARAKA N. Daniel, MONEY Marcelle, DOUMBIA Simini, CISSE Souleymane⊃, ODI Nazaire⊃ FAYE Kette Hortence, and DOSSO M.,

Application of molecular markers to find out classificatory determinants of rice (Oryza sativa L.)

January 2015

The present communication is aimed to find out determinants of molecular marker based classification of rice (Oryza sativa L) germplasm using the available data from an experiment conducted for development of molecular fingerprints of diverse varieties of Basmati and non Basmati rice adapted to irrigated and aerobic agro-eco systems. The distance-matrix based dendrogram was developed following UPGMA method that grouped...

Author(s): D. Singh, Anil Sirohi, Pooran Chand, Preeti Sirohi, Parul Sirohi, Suresh Chand , Ravinder Kumar, S.K. Mishra, Navneet Kumar, Anuj Kumar, Rajender Kumar, Atar Singh, L.K. Gangwar and Shalini Gupta

Oyster mushrooms (Pleurotus) are useful for utilizing lignocellulosic biomass

January 2015

This review shows the biotechnological potential of oyster mushrooms with lignocellulosic biomass. The bioprocessing of plant byproducts using Pleurotus species provides numerous value-added products, such as basidiocarps, animal feed, enzymes, and other useful materials. The biodegradation and bioconversion of agro wastes (lignin, cellulose and hemicellulose) could have vital implication in cleaning our environment....

Author(s): E. A. Adebayo, and D. Martínez-Carrera

Cloning of nis gene and Nisin purification from Lactococcus lactis subsp. lactis Fc2

December 2014

The present study was focused on nisin produced by Lactococcus lactis subsp. lactis (Fc2 isolate).  The isolate was identified by 16S rRNA using specific universal primer. The obtained cell-free supernatant inhibits the growth of Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Bacillus subtilis by using agar diffusion assay. Nisin structural gene was...

Author(s): D. E. El-hadedy and Eman W. El-gammal

Promoting increased Chlorella sorokiniana Shih. et Krauss (Chlorophyta) biomass production using Moringa oleifera Lam. leaf extracts

December 2014

Chlorella sorokiniana Shih. et Krauss, a unicellular green alga was assayed to assess its to promotion potentials response of aqueous and ethanolic leaf extracts of Moringa oleifera Lam. C. sorokiniana grown in 200 ml aliquots of modified basal medium for two weeks: was treated with the aqueous and ethanolic extracts at 10, 20, 30, 40 and 50% concentrations, respectively. A control was set up without Moringa extract and...

Author(s): NWEZE Nkechinyere Onyekwere and NWAFOR Felix Ifeanyi

Phytoextraction potential of cadmium and lead contamination using Melia azedarach and Populus alba seedlings

December 2014

Vegetative growth, biomass, chemical content and uptake of cadmium (Cd) and lead (Pb) in Melia azedarach L. (chinaberry) and Populus alba L. (white poplar) seedlings were investigated using a 2-year pot experiment. The results indicated that P. alba and M. azedarach are tolerant to contaminated soil by Cd or Pb without any toxicity symptoms. Vegetative growth and chemical properties of M. azedarach are negatively...

Author(s): Khamis, M. H., El-Mahrook, E. M. and Abdelgawad, M. A.

Karyotype evolution and species differentiation in the genus Rattus of Manipur, India

December 2014

Rattus is the most studied genus all over the world but species of the genus are not thoroughly reported from Manipur. The present paper deals with the morphometric, cytotaxonomic and phylogenetic studies of Manipur, India. The different species of Rattus namely Rattus rattus, Rattus brunneusculus, Rattus tanezumi and Rattus nitidus were studied. Berylmys manipulus and Berylmys mackenziei were taken from the genus...

Author(s): Chingangbam Dh., J. M. Laishram, Naorem Brajendra Singh, Loidang Taibangjam and Chingakham Brajakishore

Identifying salt stress-responsive transcripts from Roselle (Hibiscus sabdariffa L.) roots by differential display

December 2014

No previous study has been reported on the salt-modulated gene(s) of roselle (Hibiscus sabdariffa L.). Identifying the potentially novel transcripts responsible for salt stress tolerance in roselle will increase knowledge of the molecular mechanism underlying salt stress responses. In this study, differential display reverse transcriptase PCR (DDRT-PCR) was used to compare the overall differences in gene expression...

Author(s): Bahaeldeen Babiker MOHAMED, Muhammad Naveed SHAHID, Bushra RASHID, Adil JAMAL, Beenish AFTAB, Abdelhafiz Adam DAHAB, Sarfraz KIANI, Muhammad Aleem ASHRAF,  Muhammad Bilal SARWAR, Sameera HASSAN and Tayyab HUSNAIN

Quantification of the gene expression of bell peppers (Capsicum annuum) ripening gene(s) using real -time PCR

December 2014

Fruits can be divided into two groups according to the regulatory mechanisms underlying their ripening process. The two ripening processes are climacteric and non-climacteric process; bell peppers are part of the non-climacteric plant groups. Bell peppers are members of the Solanacaea family. The Solanacaea family is best known for its fruits around the world. Today’s main focus is targeted to fruit ripening, in...

Author(s): Osama S. Hassan, Fatma Badie and Gehan Safwat

Hydrogen 1 (1H) magnetic resonance spectroscopy (MRS) of 3.0T in assessment of steatosis to antiviral therapy for chronic hepatitis C

December 2014

This study investigates the utility of Hydrogen 1 (1H) magnetic resonance spectroscopy as a noninvasive test for steatosis of response to interferon and ribavirin treatment in patients developing different severity of hepatitis C virus (HCV). Ninety chronic hepatitis C patients undergoing antiviral therapy with interferon and ribavirin underwent 1H MR spectroscopy at 3.0T before treatment, 6 month after the start of...

Author(s): Qian Zhang#,Yonggui Zhang#, Chunyu Zhang, Ping Zhao, Jian Jiao and Jiangbin Wang

Determination of aflatoxin B1 in food products in Thailand

December 2014

Aflatoxin B1 is a secondary metabolite of Aspergillus flavus and Aspergillus parasiticus. It can be formed in commodities before and after harvest. This mycotoxin possesses a variety of toxic effects, potent carcinogen to both animal and human health. Aflatoxin B1 is generally found in feed and food stuff, such as cereal and all products derived from cereals, including processed cereals since it has been proven to be at...

Author(s): K. Charoenpornsook and P. Kavisarasai

Callus induction and RAPD analysis of Simarouba glauca DC

December 2014

Callus induction for somatic embryogenesis from Simarouba glauca DC leaf explants of three genotypes (S. glauca 5, S. glauca 19 and S. glauca 21) was studied. Leaf explants (leaf segments from basal, middle and tip of the leaves) were cultured on two types of nutrient media; SGC1 and SGC2. Both media contained Murashige and Skoog (MS) medium with vitamins: 100 mg/L ascorbic acid, 0.5 mg/L 6-benzylaminopurine (BAP), 0.5...

Author(s): Jyothi Kakuturu , Josekutty PC, Shobha Potlakayala, Mathew Reitzel, Kinza Salim, Subhashini Charyulu, Rafiyat Adeyiga , Shyam Menon, Stephen L. Goldman , Pooja Patel, Michael J. Chorney ,  and Sairam Rudrabhatla

Seasonal influence on sexual hormones and semen plasma parameters of Arabian sand gazelles (Gazalla subgutrosa marica) in Saudi Arabia

December 2014

The plasma levels of testosterone (T), follicular stimulating hormone (FSH) and luteinizing hormone (LH) were evaluated in Arabian sand gazelles Gazalla subgutrosa marica to reveal their relation with the sexual desire and testicular efficiency. The levels of those hormones fluctuated over the year; the FSH peak (2.212 ml/mlu) was in October (the beginning of reproductive season). LH level peaked in November (0.73...

Author(s): Abdullah A. AlKahtane

Selective isolation and screening of fungi with herbicidal potential and evaluation of herbicidal activity against Vernonia species

December 2014

In irrigated agriculture, weed control through chemical herbicides, creates various environmental and health hazards which lead to the search for an alternate method of weed management, which is an eco-friendly and effective means. Biological control of weeds using microorganism is now extensively studied to control various economic important weeds. In the present study, herbicidal activity of crude metabolites...

Author(s): S. Karthick Raja Namasivayam, A. Aruna and Shreya Hazra Choudhury

Partial purification and some physicochemical properties of Aspergillus flavus α-amylase isolated from decomposing cassava peels

December 2014

α-Amylase is one of the most important enzymes of great significance due to its wide area of potential application in food, fermentation, textile, paper, detergent and pharmaceutical industries. This study aimed at production of α-amylase from an indigenous fungal source and also ascertaining the properties of the enzyme for maximal activity. The enzyme was isolated from decomposing cassava peels,...

Author(s): Muinat Moronke Adeyanju, Oyesola Ojewunmi, Temitope Akande, Esther Nkechi Ezima, Abosede Abolanle Akeredolu and Bamidele Sanya Fagbohunka

An active form of calcium and calmodulin dependant protein kinase (ccamk) of Medicago truncatula

December 2014

The DMI3 gene of the model legume Medicago truncatula encodes a calcium and calmodulin dependent protein kinase (CCaMK) involved in the signalling pathways leading to the establishment of both mycorrhizal and rhizobial root symbiosis. The removal of the auto-inhibitory domain that negatively regulates the kinase activity in M. truncatula results in a constitutively-active form, inducing symbiotic responses in the...

Author(s): S. Kassouar and M. B. Baba Hamed

In vitro anthelmintic effects of crude aqueous extracts of Tephrosia vogelii, Tephrosia villosa and Carica papaya leaves and seeds

December 2014

The prevalence of anthelmintic resistance and the consumer demand for alternative farming systems that limit the use of chemical anthelmintics has made the search for alternative gastrointestinal nematode parasites control methods crucial. Traditional medicinal/herbal plants can offer an alternative to the reliance on chemical anthelmintic drugs. This study evaluates the efficacy of crude aqueous extracts of Tephrosia...

Author(s): C. Odhong’, R. G. Wahome, M. Vaarst, S. Nalubwama, M. Kiggundu, N. Halberg and S. Githigia

Thinking out of the box: MADS-box genes and maize spikelet development

December 2014

Due to the importance of maize as an agricultural crop and its stature as an ideal model plant for the study of developmental biology in monocots, it is natural that research into its genetic structure has gained worldwide attention. Unfortunately, although much progress has been made in our understanding of the genetic control of the maize spikelet over the last decade, the depth of research in this field still lags...

Author(s): Na Li,#, Yanfei Liu,#, Ming Zhong, Min Jiang and Haoge Li,

Anti-toxoplasma gondii activity of constituents from Balsamocitrus camerunensis L (Rutaceae)

December 2014

Isolation, characterization and anti-Toxoplasma gondii activity of constituents from the CH2Cl2/MeOH (1/1) extract of the roots of the cameroonian plant Balsamocitrus camerunensis L. were investigated in this study. Four known coumarins derivatives were isolated, namely, marmin (1), imperatorin (2), xanthoxyletin (3), 6,7-Dimethoxycoumarin (4) and an acridone derivative namely 1-hydroxy-3-methoxy-acridone (5). Their...

Author(s): Emmanuel Ngeufa HAPPI, Emmanuel Jean Teinkela Mbosso, Edwige Laure Nguemfo, Henri Réné ZAMBOU and Anatole Guy Blaise Azebaze

Effect of three types of composts of olive oil by-products on growth and yield of hard wheat "Triticum durum Desf.”

December 2014

The extraction of olive oil generates colossal by-products quantities; generally unexploited and causes serious environmental problems in Algeria. In tackling this problem, we performed three types of composts (C1: 50% cows manure, 50% olive husks and olive mill wastewaters; C2: 50% olive husks treated by the lime, 50% cows manure with fresh urea and olive mill wastewaters; C3: 50% olive husks, 50% cows manure and...

Author(s): MEDJAHDI Nadjet, DJABEUR Abderezzak and KAID-HARCHE Meriem

Screening and detection of extracellular cellulases (endo- and exo-glucanases) secreted by filamentous fungi isolated from soils using rapid tests with chromogenic dyes

December 2014

The screening plate method is commonly used for previous detection of cellulases produced by micro-organisms with biotechnological potential. In this manuscript, the authors aim to evaluate the hydrolytic ability of different fungi isolated from soil for the production of cellulolytic enzymes for cellulose degradation and determining the enzymatic index (EI) in relation to the growth of fungal colony and halo. The fungi...

Author(s): Brigitte Sthepani Orozco Colonia and Aloísio Freitas Chagas Junior

Use of pectin in the postharvest conservation of tangerine

December 2014

The aim of the present study was to evaluate the postharvest behavior of tangerine coated with different pectin concentrations during storage under controlled temperature (22°C ± 0.1). Fruits with green color (± 90% of the surface) were divided into four groups: fruits without any coating (T1) and fruits coated with pectin solution at 4 g / 100 g (T2), 6 ml / 100 ml (T3) and 8 g / 100 ml (T4)....

Author(s): Plácido, G. R., Silva, R. M., Silva, M.A.P., Furtado, D. C., Santos, M. G.; Caliari, M. and Oliveira, D. E. C.

Diversity analysis of Rhizoctonia solani causing sheath blight of rice in India

December 2014

Sheath blight (Rhizoctonia solani) causes severe loss in the production of rice in India. Twenty five (25) isolates were collected from different parts of India. Morphological and cultural characters were investigated, on the basis of colony colour, growth pattern, hyphal width, colony growth, formation of sclerotia, size of sclerotia, diameter and number of sclerotia. Pathogenic variability was studied on highly...

Author(s): Mehi Lal, Vivek Singh, Janki Kandhari, Pratibha Sharma, Vinay Kumar and Shiv Murti

Characterization of pathogen responsible for infection in bhindi plant by using phytoplasma specific universal primers

December 2014

Infection in bhindi plant (Abelmoschus esculentus, Family-Malvaceae) is very common in Gorakhpur district of Eastern U.P. region of India. To detect the pathogen, genomic DNA was isolated from the infected bhindi plant sample and amplified through polymerase chain reaction (PCR) by using P1 forward primer and Tint reverse primer, which were phytoplasma specific universal primers. Obtained PCR amplicons were cloned...

Author(s): Richa Tiwari, Sudhanshu Tiwari and P. P. Upadhyaya

Effect of elicitation on picrotin and picrotoxinin production from in vitro products of Picrorhiza kurrooa

December 2014

Picrorhiza kurrooa Royel ex. Benth. is an important medicinal plant of Himalayan region and a good source of iridoid glycosides. Picrotin and picrotoxinin are compounds produced by P. kurrooa which are widely used in treatment of hepatic diseases. Elicitation is one of the best effective methods which enhance secondary metabolite production in plants.  In the present study effect of elicitors for example, Methyl...

Author(s): Janhvi Mishra Rawat, Balwant Rawat, and Susmita Mishra

Genetic characterisation of avocado (Persea americana Mill.) in two regions of Ghana

December 2014

Avocado (Persea americana Mill.) is a nutritious and economic tree crop with cultivations scattered all over Ghana. Although, there have been some genetic diversity research on this crop in some parts of the world, rather limited research has been done on avocado in Ghana. A study was therefore conducted to assess its genetic diversity in the Ashanti and Central Regions of Ghana. Microsatellites analyses revealed 115...

Author(s): Janice Dwomoh Abraham, and Jemmy F. Takrama

Fusarium branch blight on highbush blueberry in Argentina

December 2014

In Argentina, highbush blueberry (Vaccinium corymbosum) is grown in Tucumán, Entre Ríos and Buenos Aires provinces. In the 2010 to 2011 cropping season, a new disease with 10% incidence was observed on the foliage of “Emerald” plants in Concordia, Entre Rios. Symptoms included acropetal blight and premature branch death. Leaves remained attached and showed tan to brown discoloration. A fungal...

Author(s): Eduardo Roberto Wright, Marta Carolina Rivera, Eduardo Raúl Campanella, Omar Marcelo Farinon, Marcelo Facundo Berretta and Beatriz Alida Pérez

Comparative study on early detection of sugarcane smut (Sporisorium scitamineum) by polymerase chain reaction (PCR) and microscopy

December 2014

Sugarcane smut caused by Sporisorium scitamineum has the potential to result in substantial tonnage loss and significant reduction in sucrose recovery. As early and precise diagnosis is an integral component in the successful management of sugarcane smut, the present study was undertaken to accurately determine the presence of pathogen employing PCR-based methods supplemented with microscopy. Healthy sets of sugarcane...

Author(s): M. Kavitha, A. Ramesh Sundar, P. Padmanaban, R. Viswanathan and P. Malathi

Variants of NAT2 polymorphisms: Intra and inter-ethnic differences

December 2014

N-Acetyltransferase 2 (NAT2) gene is known for its polymorphism. The genetic variations leads to the change in the N-acetylation activity and these differences in the acetylation activity leads to the classification of the population into various groups such as rapid, intermediate and slow acetylators. In the present study, we identified different mutations and alleles by sequencing a stretch of 927 bp which covered...

Author(s): Neelam Chauhan, and Harish Padh,

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