African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 407

AJBR Articles

Effects of ethanol and aqueous leaf extracts of Pterocarpus mildbraedii on hematology parameters and cholesterol in albino rats

June 2017

One of the commonly consumed leafy vegetables in Nigeria is Pterocarpus mildbraedii Harms (Leguminosae) leaf. It has been found that, ethanol and aqueous extracts of the plant leaves exert anti-diabetic effect on rats. This study investigated the effects of ethanol and aqueous leaf extracts of this plant on some hematological parameters and serum cholesterol level in Wistar albino rats. Intraperitoneal administration of...

Author(s): Adegbite A. Victor and Ezekwesili C. Nonyelum

Antimibacterial activity of saponins from Sanseviera trifasciata prain cv. golden hahnii roots on Escherichia coli and Staphylococcus aureus

May 2017

Sansevieria-trifasciata-is commonly known as both in and out door house plant. It is also used in traditional medicine for treatment of influenza, cough and respiratory inflammation. The roots and leaves have secondary metabolites such as saponins that exbihit remedy for cough, snake bite, sprain, bruise, boil, abscess, respiratory inflammation and hair tonic.  The plant sample was collected and dried. The...

Author(s): Whika Febria Dewatisari, Subandi and Desmawati

Effects of date fruit extract on paracetamol induced nephrotoxicity in wistar rats

April 2017

This study was aimed to investigate the protective, ameliorative and preventive effects of aqueous Date fruit extract on paracetamol induced nephrotoxicity in wistar rats. A total of 30 male albino wistar rats were used for this study. Rats were randomly divided into five groups containing 6 animals each. Treatments were given daily for fourteen days. The animals in the control group (Group I) did not receive any...

Author(s): Tijani Idris Ahmad Oseni, Nurah Tijani Oseni, Hauwa Tijani Oseni and Pauline Etuaje Eromon

GC-MS/FT-IR screening of Xylopia aethiopica (Dunal) A. Rich Fruit

March 2017

The present study was aimed at identifying the functional groups and phyto-constituents present in Xylopia aethiopica (Dunal) A Rich fruit using Fourier Transform Infrared Spectrometry (FTIR) and Gas chromatography-mass spectroscopy (GC-MS), spectroscopy. FTIR method was performed using Perkin Elmer Spectrophotometer and the characteristic peaks were detected. The phytochemical constituents were screened by GC-MS method...

Author(s): Okereke S. C., Arunsi U. O. and Nosiri C. I.

Antioxidative activity of Tamarindus indica L. extract and chemical fractions

February 2017

Tamarindus indica L is a tropical plant that is used around the world mainly for medicinal purposes. Yet few scientific studies of its biological potential have been undertaken. The aim of this study was to assess the antioxidative potential of T. indica L leaves. The plant material was harvested and then dried in the absence of direct sunlight. The extraction was done by alcoholic decoction, and the fractions were...

Author(s): Amadou Ibrahima Mbaye, Papa Madieye Gueye, Alioune Dior Fall, Modou Oumy Kane, Kady Diatta Badji, Abdou Sarr, Daouda Diattara and Emmanuel Bassene

Effects of Xylopia aethiopica fruit extract on isolated rabbit ileum

January 2017

In this study, pharmacological reactivity of rabbit ileum to crude extract of fruits of Xylopia aethiopica in the presence of other agonist and antagonist agents was evaluated. The contractility of the isolated rabbit ileum was recorded using kymograph. The effects of the crude extract of X. aethiopica on rabbit ileum were independently determined using a dose range of 10-8 to 10-4 mg/ml. However, to determine the...

Author(s): Okwari O.O, Obembe A.O, Jeje S.O, Honesty E.E and Osim E.E

Effect of pre-harvest chitosan foliar application on growth, yield and chemical composition of Washington navel orange trees grown in two different regions

November 2016

The present study was carried out during 2012 and 2013 seasons to study the effect of pre-harvest foliar application of chitosan (a natural beta-1-4-linked glucosamine polymer) at two concentrations 250 and 500 ppm on vegetative tree growth, fruit yield and quality as well as leaves chemical composition of Washington navel orange trees grown under two  locations. As for growth parameters (shoot length, leaves...

Author(s): Ahmed Hussien Hanafy Ahmed, Mohamed Ramadan Aboul-Ella Nesiem, Hesham Ali Allam and Amira Fahmy El-Wakil

Extraction, optimization and characterization of crude polysaccharides from Artemesia Mongolica

November 2016

In this study, the extraction conditions of polysaccharides of Artemisia mongolia (PAM) were optimized and their molecular weight, weight distribution, composition and antioxidant activities of the polysaccharides investigated. The degree of polymerization of fructan-oligosaccharides 2-8 was separated by thin layer chromatography (TLC) with good results. Polysaccharides were extracted from the leaves of A. mongolia and...

Author(s): Yahaya Kudush Kawa, Quinck Shang, Shangyi Tian, John Paul Kaisam and Victoria Kabia

Protease inhibition in cowpea pod-sucking bug species (Hemiptera: Heteroptera) using cereal and legume grain extracts

October 2016

Selected grain extracts were assayed against digestive proteases of Anoplocnemis curvipes, Clavigralla tomentosicollis and Mirperus jaculus with a view to identifying suitable sources of protease inhibitors against the cowpea pests. Laboratory bioassays showed that grains of a local soybean variety Kìshí and a cowpea landrace NG/SA/07/0098 contain potent inhibitors although the former produced a...

Author(s): Abiola Elfrida Taiwo, Olalekan Joseph Soyelu and Raphael Emuebie Okonji

Protective effects of extract and fraction of root- bark of Garcinia kola (Heckel) on the renal biochemical parameters of gentamicin-induced nephrotoxic rats

September 2016

The study evaluated the nephroprotective potentials of methanolic extract (ME) and dichloromethane fraction (DCMF) of root-bark of Garcinia kola on gentamicin-induced nephrotoxicity in rats with a view to utilize the extracts of the root of G. Kola in the amelioration of antibiotic-induced kidney damage. The study involved preparation of the extract, fractionation, phytochemical screening and evaluation of phenolics and...

Author(s): Komolafe, Ige Joseph, Akinlalu, Akintunde Oluseto, Ogunsusi, Mayowa and Oyedapo, Oluboade Oluokun

Oil palm fruit calyx as a resource for phospholipids extraction

May 2016

Oil palm calyx is a solid waste in the oil palm industry which is yet to find economic usage. This study aimed to determine the feasibility of recovering phospholipids from the oil palm calyx. Preliminary functional property of calyx phospholipids was determined using emulsion stability. Results indicated that oil palm calyx had 14% total lipids when extracted with methanol and 62.3% of lipids were acetone insoluble...

Author(s): Gold I. L., Oseni N. T. and Imoisi O. B.

Antihyperlipidaemic and anti-atherosclerotic activities of aqueous leaf extract of L. africana in streptozotocin induced diabetic rats

March 2016

Hyperlipidaemia is a usual and life threatening complication of diabetes mellitus. Consequently, a good anti-diabetic agent should reduce the progression of this complication. In view of this, the anti-hyperlipidaemic and anti-atherogenic activities of aqueous extract of Lasianthera africana was studied in streptozotocin induced diabetic rats. Extract treatment was administered at a dose of 1ml of 180 mg/ml of extract...

Author(s): Uwakwe Augustine  A., Miikue-Yobe Togenu F. B., Akaninwor Joyce O. and Felix-Samuel Barisi

Bio-enrichment of phenolics and free radicals scavenging activity of wheat (WH-711) fractions by solid state fermentation with Aspergillus oryzae

February 2016

This study was conducted to determine the total phenol content (TPC) and free radicals scavenging activity of methanolic and ethanolic extracts of wheat (WH-711) fractions (grain, bran and flour) fermented with Aspergillus oryzae (MTCC 3107). Maximum TPC (1469.35 ± 4.5, 1426.44 ± 5.5 and 1268.91 ± 5.3) was obtained in methanolic extracts as compared to ethanolic extracts (1368.65 ± 3.5,...

Author(s): Joginder Singh Duhan, Kamal Mehta, Pardeep Kumar Sadh, Pooja Saharan and Surekha

Effect of kidney bean consumption on some lipid and haematological parameters of albino rats

January 2016

This study investigated changes in cholesterol levels and haematological parameters of rats subjected to diets supplemented with kidney bean seeds. The seeds were processed in three parts; the first part was milled raw; the second part was boiled, dried and milled; third part was germinated, dried and milled. The kidney bean seed flour was incorporated into the diet of different groups of rats at 10% inclusion. Thirty...

Author(s): Olanipekun, O. T., , Balogun, E. A., Akinloye, O. A., and Omotainse, S. O.

Modulation in membrane lipids of Escherichia coli and Staphylococcus aureus in response to varying NaCl concentrations

January 2016

The effect of varying NaCl concentrations on membrane lipids of Escherichia coli and Staphylococcus aureus cultivated at 37°C was studied. Lipid profiles of the membrane extracts were resolved by thin layer chromatography (TLC) using polar and neutral lipids solvent systems, with their fatty acid constituents determined via gas chromatography-mass spectrometry (GC-MS). Polar lipids identified were cardiolipin,...

Author(s): Omotoyinbo Oluwasegun Victor, Sanni David Morakinyo and Olawoye Theophilus

Production and antioxidant activity of alcoholic beverages made from various colored rice and wild rice

December 2015

In order to develop a new type of alcoholic beverage with antioxidant activity, the authors carried out this research. In this study, alcoholic beverages were made from unpolished black rice, unpolished red rice, unpolished green rice, polished white rice and unpolished wild rice using conventional cooked fermentation and economical uncooked fermentation system. The resulting alcoholic beverages made from cooked and...

Author(s): Ryoichi TAKESHITA, Noriaki SAIGUSA and Yuji TERAMOTO

Nitrate contents in some vegetable leaves in Sokoto Metropolis, Nigeria

November 2015

Popular Nigerian vegetables namely, cabbage, spinach, bitter leaf, water leaf, ewedu, roselle and lettuce obtained from Sokoto metropolis, Nigeria were analysed for nitrate contents by ultra-violet (UV)-spectrophotometric method. The fresh leaves of the samples were chopped and ground using mortar and pestle for the analysis. Absorbance of each sample was obtained in three replicate and the calibration graph of...

Author(s): Abdullahi, A. S., Usman, J., Muazu, S., Abba, Y. and Ibrahim, M. K.

Extraction, fractionation and assessment of antioxidant activities of active components of Aframomum sceptrum seeds

November 2015

A simple procedure of extraction, fractionation and assessment of organoleptic and antioxidant properties of Aframomum sceptrum seeds was carried out, and the results were compared with a currently used synthetic antioxidant, butylated hydroxy toluene (BHT). Extraction was carried out using Soxhlet extractor and fractionation of the ethanolic extract was accomplished using vacuum liquid chromatography (VLC). Various...

Author(s): Nwankwo Patience Okwuchi

Comparative effects of peel extract from Nigerian grown citrus on body weight, liver weight and serum lipids in rats fed a high-fat diet

October 2015

The effects of ethanolic extracts of five different citrus peels on mean body and liver weight and serum lipid content were investigated in albino rats. Six groups (n=8 each) were fed with a high fat diet for seven days ad libitum before oral daily administration of the peel extracts of orange (OR), lemon (LE), lime (LI), tangerine (TA), grapefruit (GR) and synergistic combination of equal ratios (w/w), that is, (SY),...

Author(s): Josephine Ozioma Ezekwesili-Ofili and Ngozi Christine Gwacham

Production of bacterial amylases and cellulases using sweet potato (Ipomoea batatas. (L.) Lam.) peels

October 2015

Peels of sweet potato (Ipomoea batatas) were buried in the soil for 14 days and the isolates associated with the degradation of the peels were obtained using standard microbiological procedures. The bacterial isolates obtained were screened for amylolytic and cellulolytic activities under different pH and temperatures as parameters and optimized for enzyme production. Sixteen (16) bacterial isolates were obtained and...

Author(s): Olanbiwoninu Afolake Atinuke and Fasiku Samuel 

Association of single nucleotide polymorphism of Hsp90ab1 gene with thermotolerance and milk yield in Sahiwal cows

September 2015

Heat shock proteins play a critical role in the development of thermotolerance and protection from cellular damage associated with heat stresses. The study was undertaken to investigate the association of single nucleotide polymorphisms (SNPs) of Hsp90ab1 gene with thermo-physiological parameters viz, respiration rate (RR), rectal temperature (RT), heat tolerance coefficient (HTC) and total milk yield in Sahiwal cows....

Author(s): Lalrengpuii Sailo, I. D. Gupta, Archana Verma, Ramendra Das and M. V. Chaudhari

The effect of aqueous leaf extract of fluted pumpkin on some hematological parameters and liver enzymes in 2,4-dinitrophenylhydrazine- induced anemic rats

August 2015

Anemia constitutes a serious health problem in many tropical countries including Nigeria because of the prevalence of malaria and other parasitic infections which possibly leads to decrease of hemoglobin. Fluted pumpkin has been reported to be very good in building the constituents of the blood and also replacing them. This study was designed to investigate the effects of the aqueous leaf extract of fluted pumpkin on...

Author(s): Toma, I., Victory, N. C. and Kabir, Y.

Effect of fermentation methods on the mineral, amino and fatty acids composition of Cyperus esculentus

August 2015

Tiger nut (Cyperus esculentus) was subjected to different fermentation methods such as traditional, back slope and control. The raw and fermented samples were analyzed for mineral, amino and fatty acids. The results of mineral analysis revealed potassium and sodium as the most abundant mineral element with their value ranging from 546 to 91.6 mg/100 g and 64.00 to 3383.33 mg/100 g, respectively while copper was found in...

Author(s): Agbaje, R. B., Oyetayo, V. O. and Ojokoh, A. O.

Diastatic enzymes malting process optimisation of African finger millet for biotechnological uses

July 2015

This study sought to understand the importance of variation of steeping and germination conditions (temperature, pH and salts) on the quality of African finger millet malt in terms of diastic power (a-amylase and β-amylase), endo- (1,3) (1,4)-β-D-glucanase, b-glycan content and protein profile. The results show that the physiological responses of African finger millet malted seeds are correlated to pH (acidity...

Author(s): Adejoke N. Kolawole and Ayodele O. Kolawole

Purification and biochemical properties of lipase from raphia palm fruit mesocarp

May 2015

Lipases hydrolyze lipids to yield fatty acids and glycerol which are used as raw materials in industries. The lipase from raphia mesocarp was extracted, purified and characterized. Assay was carried out using p- nitrophenyl palmitate as substrate. The lipase was subjected to 80% ammonium sulphate precipitation, purified on ion exchange and gel filtration chromatography. Native and subunit molecular weights were...

Author(s): T.  Okunwaye, J.U. Obibuzor and E.A. Okogbenin

Biochemical changes in blood and tissues of Wistar rats following administration of anti-tuberculosis drugs

April 2015

Tuberculosis (TB) remains a major public health problem worldwide especially in resource-limited countries of Africa and Asia. The disease is curable with drugs but information on biochemical changes and oxidative indices due to anti-TB drugs is not readily available. This study was designed to investigate in vivo effects of anti-TB drugs in male Wistar rats. Fourteen adult male rats were randomly divided into two...

Author(s): Aderemi Oludiran Kehinde, and Oluwatosin Adaramoye

Detection of DNA damage in lead (Pb) exposed city traffic wardens in Pakistan

April 2015

Lead (Pb) is one of the toxic metals and is commonly used in industries. It affects multiple systems and has role in the production of reactive oxygen species (ROS). The objective of the present study was to determine Pb levels and to detect DNA damage in traffic wardens of Lahore, Pakistan. A total of 90 subjects were selected including 60 traffic wardens working in field and 30 working in administrative zones. The...

Author(s): Ayesha Pervez, Fozan Ahmed, Nazish Mehmood Aisha, Salman Idrees, Muhammad Ikram Ullah, Mohammad Zamir Ahmad, Aftab Ahmed and Muhammad Jawad Hassan

Diabetes and pre-diabetes in adult Nigerians: Prevalence, and correlations of blood glucose concentrations with measures of obesity

March 2015

The quantification of the number of people with diabetes is an important factor that allows for effective planning and distribution of scarce resources for the management of diabetes. This study therefore investigated the prevalence of diabetes and impaired fasting glucose in a convenient population of adults in Umudike, a semi-urban town in South-East Nigeria. The relationship between measures of obesity and blood...

Author(s): Chukwunonso E. C. C.  Ejike, Nnamdi K. Uka and Stella O. Nwachukwu

Plasma lipid profile including the high density lipoprotein (HDL) subclasses in hypertensive patients in Ouagadougou, Burkina Faso

March 2015

The study was undertaken to evaluate the interest of cholesterol subclasses in the management of hypertensive patients recruited at University Hospital Yalgado Ouedraogo of Ouagadougou (Burkina Faso, West Africa). The distribution of hypertensive was reported as 45 (35.4%) without complications, 42 (33%) with cardiovascular complications and 40 (31.4%) with diabetes. Any difference in lipids profile was observed when...

Author(s): Alice T. C. R. Kiba Koumaré, Absetou Guira, André Samandoulougou, Elie Kabré, Issaka Sondé, Talkmore Maruta, Jacques Simporé and Jean Sakandé

Effect of some traditional edible oils on sex hormones of albino Wistar rats

March 2015

The effect of some traditionally extracted edible seed oils (sesame, peanut and melon oils) on some sex hormones - prolactin, progesterone, testosterone, estradiol, luteinizing hormone (LH) and follicle stimulating hormone (FSH) of albino Wistar rats was studied. Sixty (30 - 50 g) weanling rats (20 males and 40 females) were purchased and housed separately until they weighed 120 to 150 g. Afterwards, the rats were...

Author(s): Margaret Akpana Agiang, Olajumoke Josephine Matthew, Item Justin Atangwho and Patrick Ekong Ebong

Characterization of partially purified cysteine protease inhibitor from Tetracarpidium conophorum (African walnut)

February 2015

Cysteine protease inhibitors (CPIs) have been known to be present in a variety of seeds of plants, and have been intensively studied as useful tools for potential utilization in pharmacology. This study reports the isolation of CPI from Tetracarpidium conophorum by 65% ammonium sulphate saturation, followed by ion exchange chromatography; further purification was by gel filtration chromatography. The molecular weight of...

Author(s): Obayomi, A., Adeola, S. A., Bankole, H. A. and Raimi, O. G.,

Glycated proteins: Clinical utility and analytical approaches

February 2015

The binding of glucose to serum proteins in a non-enzymatic reaction occurs proportionately to the prevailing serum glucose concentration during the lifespan of each protein. The non-enzymatic reaction of proteins with reducing sugars results in the formation of glycated proteins that are indicative of the relative amount of reducing sugars present. Hemoglobin, albumin, lipoproteins and other tissue proteins can be...

Author(s): Yoseph Cherinet Megerssa and Demo Yemane Tesfaye

Effects of sound waves on the enzyme activity of rice-koji

February 2015

Rice-koji is an important agent for the saccharification of fermented food production in Japan. In koji-making, temperature, humidity, light and oxygen are important environmental factors. However, there is no report of koji-making controlled by sound wave. In this study, focusing on the enzyme activity of rice-koji, we confirmed the relationship between enzyme activity and frequency of sound waves. Here, the enzyme...

Author(s): Noriaki Saigusa, Seika Imayama and Yuji Teramoto

Higher tolerance of a novel lipase from Aspergillus flavus to the presence of free fatty acids at lipid/water interface

January 2015

The main objective of this work was to identify novel lipases of industrial interest. In this paper, Aspergillus flavus lipase (AFL) was isolated from the traditional tannery of Fez city in Morocco; it kept its stability even in the presence of high concentrations of detergent from 0 to 10 mM sodium deoxycholate (NaDC). Bile salts showed no inhibitory effect on the lipolytic activity, whereas the calcium salts showed a...

Author(s): Faouzi Laachari, Fatimazahr​a El Bergadi, Moulay Sadiki, Adel Sayari, Wifak Bahafid, Soumya Elabed, Iraqui Mohammed and Saad Koraichi Ibnsouda

Changes in enzymes activities of soil samples exposed to electromagnetic radiations (EMR) from mobile phone

January 2015

This study aimed at investigating the changes in activities of dehydrogenase, catalase, alkaline phosphatase, acid phosphatase and alkaline protease of soil samples exposed to electromagnetic radiations (EMR) from mobile phone for the periods of 0, 30, 60 and 90 days. EMR-unexposed soil samples served as the source of control enzyme activities. The mean enzyme activities from the EMR-exposed soil were significantly...

Author(s): C. I. Iheme, R. N. Nwaoguikpe, S. E. Abanobi, C. U. Igwe, E. C. Udenze, A. U. Ezirim, C. P. Ihedimbu and D. I. Ukairo

Radiation protection and anti-oxidative effects of garlic, onion and ginger extracts, x-ray exposed albino rats as model for biochemical studies

November 2014

The present study investigates and examines the comparative effects of plant extracts such as, garlic, ginger and onion on some organs (liver, kidney and heart) of x-ray exposed rats, using and assaying some biochemical enzymes. Twenty (20) albino rats with an average weight of (155.00 ± 2.01 g), divided into five groups were used for the study. The rats with exception of the control were exposed to x-ray with...

Author(s): Kenneth C. Nwachukwu, Samuel O. Asagba, Chibueze Nwose and Michael P. Okoh

A study of two weeks administration of copper sulphate on markers of renal function and feeding pattern of Wistar rats

November 2014

This study aimed at determining the changes in food consumption, water intake, plasma and urine concentrations of some organic constituents which are often used in the assessment of renal function following two weeks’ administration of two doses of copper sulphate to Wistar rats. Fifteen adult male Wistar rats were randomly divided into three groups of five rats each. Group I (control group) received distilled...

Author(s): Rufus O. Akomolafe, Olaoluwa S. Olukiran, Chris E. Imafidon, Olugbengba A. Ayannuga, John A. Oyekunle, Babatunde O. Akanji and Ayowole A. Oladele

Hypolipidemic effect of Irvingia gabonensis fruits juice on sodium fluoride induced dyslipidemia in rats

October 2014

Effect of Irvingia gabonensis fruit juice administration on serum lipid profile of sodium fluoride (NaF)-intoxicated rats was investigated. Twenty-four (24) male Wistar rats divided into four groups of six (6) animals each - one control and three intoxicated groups were used. The normal control (NC) group received only standard pelletized diet and water. All three intoxicated groups received 20 mgkg-1 bodyweight of NaF...

Author(s): Emejulu, Adamma Angela, Alisi, Chinwe Sylvanus, Asiwe, Emeka Sabastine, Iheanacho, Kizito Munachiso and Onwuliri, Viola Adaku

Effect of rosemary (Rosmarinus officinalis) on lipid profiles and blood glucose in human diabetic patients (type-2)

October 2014

Investigation of the effect of oral administration of rosemary (Rosmarinus officinalis) as powder on lipid profiles and blood glucose in healthy and type-2 diabetic human patients was done. Forty-five type-2 diabetic patients and 15 non-diabetic persons of age 40 years or older participated in the study. Patients selected in this study had fasting blood glucose in the range 160-300 mg/dl, and high lipid profiles levels....

Author(s): Abdulrahim Al Jamal

Replacement of bakery shortening with rice bran oil in the preparation of muffins

September 2014

Studies were carried out to replace bakery shortening with refined rice bran oil in the preparation of muffins. Physico-chemical properties of bakery shortening and rice bran oil were studied. Rice bran oil was found to have a higher content of the essential fatty acid linoleic acid (34.98%) as compared to that of bakery shortening (5.14%). Chemical composition of wheat flour used was also studied. Muffin samples were...

Author(s): Kaur, A, Vishaldeep Jassal and Bhise, S. R.

Lead and cadmium residue determination in spices available in Tripoli City markets (Libya)

September 2014

In recent years, there has been a growing interest in monitoring heavy metal contamination in food products. Spices can improve the taste of food and can also be a source of many bioactive compounds but can unfortunately, also be contaminated with dangerous materials, potentially heavy metals. This study was conducted to investigate lead (Pb) and cadmium (Cd) contamination in selected spices commonly consumed in Libya...

Author(s): Mohamed Ziyaina, Ahlam Rajab, Khadija Alkhweldi, Wafia Algami, Omer Al-Toumi and Barbara Rasco

Safety evaluation for multispecies probiotics in a 28-day feeding study in Sprague-Dawley rats

September 2014

This experiment evaluated the 28-day feeding study in rats after they were continuously fed with multi-species probiotics dosages in the low-dosage, medium-dosage, and high-dosage groups: 2,000 mg/kg body weight (B.W.), 4,000 mg/kg B.W., and 6,000 mg/kg B.W., respectively, for 28 days as a reference for the safety of its repeated usage. Each group consisted of 10 male and 10 female Sprague-Dawley (SD) rats and the test...

Author(s): Chun-Chih Huang, Mun-Kit Nam, Yueh-Ting Tsai and Cheng-Chih Tsai

Influence of CaCl2 and EDTA on reversible thermal inactivation of recombinant wild-type and mutant (E40H/E44H) Phlebia radiata manganese peroxidase 3 (rPr-MnP3)

August 2014

Thermal inactivation of a new recombinant Phlebia radiata manganese peroxidase (rPr-MnP3) in the presence and absence of additives (CaCl2 and EDTA) is described for the first time. The influence of temperature and melting points (Tm) on the stability of rPr-MnP3 and its mutant (E40H/E44H) were determined. There was no significant inactivation at 25 – 40°C. However, we observed rapid inactivation of rPr-MnP3 at...

Author(s): Usenobong F. Ufot and Monday I. Akpanabiatu

Plasmodium berghei ANKA: Selection of pyronaridine resistance in mouse model

August 2014

Pyronaridine is a partner drug in, Pyramax®, a combination of artesunate (ASN)-pyronaridine (PRD) which was recently prequalified by WHO drug as a potential alternative for treatment of malaria in African setting. Pyronaridine is a mannich base, with a long half-life, thus predisposed to resistance. In this study, we selected pyronaridine resistance by submitting Plasmodium berghei ANKA line in vivo to increasing...

Author(s): Kimani S. K., Ng’ang’a J. K., Kariuki D. W., Kinyua J., Kimani F. T. and Kiboi D. M.,

Effects of dietary tyrosine on serum cholesterol fractions in rats

May 2014

The present study was undertaken to measure the effects of dietary tyrosine added to rat diet on serum cholesterol levels in the rat. A total of twenty Wistar strain albino rats were fed with different doses of tyrosine enriched diets containing 0.8 g/100 g, 1.0 g/100 g and 1.2 g/100 g. After 3 weeks of experimental feeding, there was significant increase (p<0.05) in total postprandial serum cholesterol of rats fed...

Author(s): ANADUAKA, Emeka Godwin, EGBA, Simeon Ikechukwu, UGWU, Jecintha Uchenna, APEH, Victor Onukwube and UGWU Okechukwu Paul-Chima

Antidenaturation and antioxidative properties of phytochemical components from Spondias mombin

May 2014

The antidenaturation and antioxidant properties of Spondias mombin Linn (Anacardiaceae) methanol leaf extract (SMC) and fractions prepared from it were evaluated in this study. SMC and its fractions: ether (SME), saponin-rich (SMS) and flavonoid-rich (SMF) were phytochemically screened and evaluated for total antioxidant activity (TAA), ability to inhibit deoxyribose degradation (DEO), lipid peroxidation inhibitory...

Author(s): Afolabi C. Akinmoladun, Olamide O. Crown, Olubukola B. Ojo, Tolulope M. Olaleye and Ebenezer O. Farombi

Properties of arginase from gut of adult cockroach (Periplaneta americana)

April 2014

Arginase (EC 3.5.3.1) catalyzes the hydrolysis of arginine to ornithine and urea. The role of arginase in insect has been seen in the growth of insect at various stages of development and energy requirement for flight. Here, we reported the properties of arginase in the gut of adult cockroach, Periplaneta americana. The enzyme was partially purified with ammonium sulphate precipitation and affinity chromatography. The...

Author(s): Okonji R. E., Ehigie L. O. and Soyelu O. J.

Aqueous Bridelia ferruginea stem bark transaminase activities in albino rats

April 2014

The major objective of this study was to evaluate the transaminase activities of albino rats fed with aqueous extract of Bridelia ferruginea stem bark. Sixteen (16) albino rats with weight ranging between 100-190 g were used. The rats were divided into four groups; with group four as the control group. Increasing doses (100, 200 and 400 mg kg-1 body weight) of the extract were administered orally to the other three...

Author(s): Olarewaju O. I., Oloyede O. I., Ojo O. A, Onikanni S. A and Ajiboye B. O

Properties of rhodanese from the liver of tilapia, Oreochromis niloticus, in Asejire Lake, Nigeria

March 2014

The study investigates the purification and characterisation of rhodanese from the liver of the tilapia fish (Oreochromis niloticus) collected from Asejire Lake in Nigeria. This was with a view to understanding the biochemical basis of the survival of the fish in cyanide polluted water. Rhodanese was isolated and purified from liver tissue homogenate of tilapia using CM-Sephadex ion exchange chromatography and Sephadex...

Author(s): Atere Tope Gafar, Ademakinwa Adedeji Nelson and Agboola Femi Kayode

Usefulness of liver and kidney function parameters as biomarkers of ‘heavy metals’ exposure in a mammalian model Mus musculus

March 2014

The toxicological evaluations of cadmium (Cd), iron (Fe), manganese (Mn), lead (Pb) and zinc (Zn) were carried out against Albino mice model, Mus musculus. On the basis of LC50 value, Cd (0.47 mM) was found to be the most toxic followed by Zn (2.40 mM), Pb (2.42 mM), Fe (4.52 mM) and Mn (5.70 mM) as least toxic. The results of the sublethal concentration of the heavy metals (1/10th of 96 hrLC50) on the liver function...

Author(s): Osuala Fidelia I., Otitoloju A. A. and Igwo-Ezikpe M. N.

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