Parameter optimization of pyoverdine content and growth kinetics on Pseudomonas fluorescens pf-10 strain in iron deficient liquid state media
February 2018
Iron nutrition in bacteria presents a fundamental challenge due to its extremely low solubility in aerobic environments at moderate pH. For optimal growth, Pseudomonas bacteria necessitate about 10 e-6 to 10 e-7 M of bioavailable iron and therefore solve an iron supply problem for survival by synthesizing and exporting a low-molecular-weight compound called siderophore. The latter has a high iron binding capability from...
Sugarcane growth promotion by Kosakonia sp. ICB117 an endophytic and diazotrophic bacterium
February 2018
The present study investigates the strain Kosakonia sp. ICB117, an endophytic, N2-fixing bacterium that belongs to a genus recently described. The bacteria were isolated from sugarcane stalks (Saccharum sp. variety SP791011) and inoculated into other sugarcane plants of the same variety. The effect of inoculation on sugarcane growth was then studied in the presence or absence of nitrate supplementation (10 mM). The...
Shelf life enhancement of plant growth promoting rhizobacteria using a simple formulation screening method
February 2018
Plant growth promoting rhizobacteria (PGPR) are a specific group of bacteria interacting beneficially with plants. Among the known PGPRs, the species Pseudomonas fluorescens and Burkholderia pyrrocinia have been highlighted in both growth promotion and control of rice diseases. Ensuring the stability of the microorganism during production, formulation, distribution and storage has been a challenge for these species. In...
Quorum sensing: An imperative longevity weapon in bacteria
January 2018
Bacterial cells exhibit a complex pattern of co-operative behaviour as shown by their capacity to communicate amongst each other. Quorum sensing (QS) is a generic term used for bacterial cell-to-cell communication which secures survival of its species. Many QS bacteria produce and release autoinducers like acyl-homoserine lactone-signaling molecules to regulate cell population density. Different species of bacteria...
Nosocomial infections are still a major concern in peri-urban polyclinics in Ghana
January 2018
The study assessed potential sources of nosocomial infections as well as the knowledge of healthcare workers about these infections in a peri-urban polyclinic of Ghana. Swabs were taken in duplicates from beds in the wards, door knobs, sinks, nurses’ desks, taps handles, delivery beds, wound dressing rooms, and door and flashing handles of lavatories. After overnight incubation, the samples were serially diluted...
Phenotypic characteristics of rhizobial and non-rhizobial isolates recovered from root nodules of chickpea (Cicer arietinum L.) grown in Ethiopia
January 2018
Fifty endophytic bacteria recovered from surface sterilized root nodules of chickpea (Cicer arietinum L.) grown in Ethiopia were authenticated using three chickpea cultivars (Natoli, ICC-4918 and ICC-5003). These isolates were characterized based on morphological and phenotypic traits. The test isolates were divided into two: bacteria nodulating chickpea (27 isolates, Group I] and endophytic non-nodulating bacteria (23...
Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry
January 2018
This study aimed to identify yeasts directly from positive blood cultures by MALDI-TOF mass spectrometry. This was a 5-month prospective study (February to June, 2015) carried out on bottles of blood cultures from in-patients at the Hôpital de l’ARCHET at the CHU, Nice. Positive blood culture broth was analysed by MALDI-TOF mass spectrometry using Sodium Dodecyl Sulphate (SDS) as the lysis buffer. Out of 64...
Antibiotic susceptibility and serotype patterns of Pseudomonas aeruginosa from clinical isolates in Abidjan, Cote d’Ivoire
January 2018
Pseudomonas aeruginosa (P. aeruginosa) has been found to be a common hospital acquired pathogen, responsible for several severe infections. The objective of this study was to describe the antibiotic resistance profile of P. aeruginosa strains from human sample. This descriptive study was carried out on 168 isolated strains of P. aeruginosa collected from January 2014 to December 2015 at the Pasteur Institute of...
GC-MS and molecular analyses of monocrotophos biodegradation by selected bacterial isolates
January 2018
Soil treatment incubation method was carried out to analyze the monocrotophos (MCP) biodegradation using three selected bacterial isolates namely Pseudomonas stutzeri (KY287931), Bacillus licheniforms (KY287928), and Bacillus sonorensis (KY287930). After 45 days of incubation of pesticide exposed to soil samples, the MCP biodegradation by individual cultures and consortium culture was analyzed and conformed by gas...
Evaluation of various marine products against Rhizoctonia solani under in vitro condition
January 2018
Rhizoctonia solani is the causative agent of rice sheath blight, which has become a major problem in rice production. Seaweed provides a rich source of structurally diverse and biologically active secondary metabolite and is proved to be better in decreasing foliar fungal diseases which ultimately increase its fertility and help in the growth of plants. The use of natural products is the ultimate way of combating this...
Phenotypic detection methods of metallo-β-lactamases -producing Pseudomonas aeruginosa strains isolated in urology ward from Skikda hospital Algeria
January 2018
Acquired metallo-β-lactamases (MβL) are emerging determinants of resistance in Pseudomonas aeruginosa . The objectives of this study were to phenotypically detect MβL in P. aeruginosa collected in urology ward from Skikda hospital Algeria. A total of seventeen P. aeruginosa isolates were identified using API 20NE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry...
Evaluation of antibacterial and phytochemical properties of different spice extracts
January 2018
Now-a-days majority of world population rely on the plant preparations as medicines to cure diseases, as they are considered safe and as effective as allopathic preparations without any side effects. Spices are plant products having aroma, are mainly used during cooking to impart flavor and taste to the dish and also possess medicinal values. The present study was designed to evaluate the antimicrobial activity of four...
In vitro antibiotic resistance patterns of Pseudomonas spp. isolated from clinical samples of a hospital in Madinah, Saudi Arabia
January 2018
Pseudomonas spp. are the leading cause of nosocomial infections. Rise in multidrug resistance among clinical isolates limit therapeutic options and hence increase mortality rate. Clinical samples (6840) from a hospital in Madinah, Saudi Arabia were collected for a duration of 14 months to study the frequency, antimicrobial sensitivity pattern and seasonal variations of Pseudomonas isolates. Conventional...
Polyphenolic profile, and antioxidant and antifungal activities of honey products in Benin
January 2018
The aim of this study was to characterize polyphenolic compounds, antifungal and antioxidant activities of polyfloral honey collected from three different phytogeographical zones in Benin duing dry and rainy seasons. Spectrophotometrically at 517 nm, DPPH scavenging activity of tested samples was measured, while antifungal activity, total polyphenols, flavonoids and condensed tannins were evaluted by Dohou, Singleton...
Analysis of nitrifying microbial community for organic hydroponics
January 2018
For many years, the generation of nitrates from organic sources in order to create nutrient solutions for hydroponics had proved a challenge until lately when microorganisms were introduced to perform this task. The objectives of the current study therefore were to use local microbial consortium to nitrify goat manure in water and to determine microbial diversity in the inoculated consortium. Therefore, microorganisms...
Genotyping of high-risk human papilloma virus (HR-HPV) and its role in cervical cancer among suspected women at reproductive age
December 2017
One of the important causes of uterine and cervical malignancy and premalignancy lesions in human is the infection with papilloma virus, especially genotypes that belong to high-risk human papilloma virus (HR-HPV) group. The involvement of HPV DNA testing in an adjacent to cytological screening will be of positive impact in early discovery of cervical neoplasia among women at risk and will significantly minimize the...
Solanum paniculatum Linn: A potential antimicrobial agent against oral microorganisms
December 2017
In Brazil, several species of potentially medicinal native plants exist, including Solanum paniculatum. This species is commonly known as “jurubeba” and belongs to the Solanaceae family, which is found in several regions in Brazil. It is widely used as a remedy for bronchitis, coughs, arthritis, jaundice, hepatitis, fever and stomach problems. The plant is believed to possess anti-viral, anti-cancer,...
Phytochemical screening and antifungal activity of leaves extracts of Luffa cylindrica (Roem)
December 2017
Mycosis constitutes a common health problem, especially in developing countries like Nigeria. The current cascade of antifungals are either too toxic or require long term use for total eradication. This study evaluates antifungal activity and phytochemical constituents of Luffa cylindrica leaves extract which were screened for the presence of bioactive phytochemicals and extracted by cold maceration in n-hexane, ethyl...
Genotypic characterization of Escherichia coli strains isolated from dairy cattle environment
December 2017
The aim of this study was to characterize the diversity of Escherichia coli strains involved in the dispersion of virulence genes. 152 E. coli strains originated from dairy cattle environment were evaluated through phenotypic and proteomic assays. These samples were investigated for the presence of virulence genes (eaeA, stxI, stxII, ST, LT, eagg, ial) and biofilm related genes (fimH, csgA, flu). Eighteen profiles were...
Phytochemical screening, toxicity and antimicrobial action of Solanum paniculatum Linn extract against dental biofilm bacteria
December 2017
The aims of study were to determine the phytochemical composition of hydroalcoholic extract of Solanum paniculatum Linn (jurubeba) root, to evaluate its in vitro antimicrobial action, as well as to determine the acute toxicity and potential cytotoxic effects of this extract. The extract was characterized by phytochemical screening and thin-layer chromatography. The following oral bacteria were used to determine the...
Variability in the genotypes of rotavirus detected in Côte d'Ivoire from 2010-2016
December 2017
Group A rotaviruses are the major viral agent of acute gastroenteritis and severe diarrhea in children <5 years old. The World Health Organization (WHO) recommends surveillance of circulating strains before and after introduction of vaccination in countries. However, the diversity of circulating strains in developing countries is a major challenge to the vaccination programs. This study, carried out in furtherance of...
A retrospective study of antibiotic sensitivity pattern of uropathogens in the Federal Capital Territory, Nigeria
December 2017
This study was designed to bring to the fore the prevalent causative agents of urinary tract infections (UTIs) and their antibiotic susceptibility patterns in Nigeria, using patients attending University of Abuja Teaching Hospital (UATH) as a case study. Using microbiological/biochemical methods, prevalence of uropathogens amongst sexes, was compared between 2010 and 2015. Bacterial counts (105/ml) in the urine was used...
Sanitization protocols applied to commercial restaurants: Effects on natural contaminant microbiota and Salmonella enterica Enteritidis adhered on tomatoes
December 2017
Sanitization is considered as essential for the microbial control of vegetables. The aim of this study was to evaluate the sanitization procedures used in commercial restaurants located in Vitória, Brazil. The efficacy of these sanitization procedures in reducing the presence of natural microbiota and Salmonella enterica Enteritidis cells on tomatoes were evaluated. All the restaurants in this study applied the...
Isolation of indigenous microorganisms from soil contaminated with metal scraps for the uptake of selected heavy metals in constituted growth media
December 2017
Indigenous bacteria and fungi were isolated from soil obtained from a metal scrap dumpsite in Ibadan, Nigeria. Soil sample analyses showed presence of calcium, iron, magnesium, zinc, manganese and copper. A selective isolation of microorganisms was done using heavy metal constituted growth media at concentration of 25 mg/L. Lactobacillus casei was isolated from cadmium and lead composed growth media, Corynebacterium...
Prevalence, risk factors and multidrug resistance profile of Staphylococcus aureus isolated from bovine mastitis in selected dairy farms in and around Asella town, Arsi Zone, South Eastern Ethiopia
December 2017
A cross-sectional study was carried out from November 2012 - May 2013 to estimate the prevalence of multi drug resistance Staphylococcus aureus from bovine mastitis and to assess its associated risk factors in selected dairy farms in and around Asella town, Arsi Zone, Ethiopia. A total of 384 fresh raw milk samples from dairy cows were examined for mastitis. Milk samples collected from 251 mastitic lactating cows were...
Serotypes and antimicrobial resistance profiles of Salmonella isolated from fresh beef processing and chilled fresh beef samples produced and marketed in the metropolitan region of Cuiabá, in the State of Mato Grosso, Brazil
December 2017
Salmonella spp. in food products of animal origin can cause foodborne infections, and when antimicrobial-resistant strains are present, can evolve to severe disease. This study aims to determine the occurrence of Salmonella and identify the serotypes present in the beef production chain of the metropolitan region of Cuiabá, State of Mato Grosso, Brazil, and evaluate their antimicrobial resistance profile. The...
Mitigation of drought in rice by a phyllosphere bacterium Bacillus altitudinis FD48
December 2017
Phyllosphere bacteria plays important role in mitigating biotic and abiotic stress. This study aimed to use phyllosphere bacterium of rice to enhance drought tolerance. Bacterial isolates obtained from rice phyllosphere were investigated for their plant growth promoting activities and role in alleviation of drought stress in rice. The isolates were identified as Bacillus altitudinis FD48, Bacillus pumilus FS20 and...
Isolation and identification of methicillin-resistant Staphlococcus aureus from mastitic dairy cows in Bishoftu town, Ethiopia
November 2017
This study determines prevalence of methicillin-resistant S. aureus from bovine mastitis in dairy cows from October 2012 to June 2013 in Bishoftu town, Ethiopia. In this cross-sectional study, 16 dairy farms were included and 384 lactating cows sampled. Clinical examination and California mastitis test (CMT) were performed to diagnose clinical and subclinical mastitis, respectively. Milk samples were obtained from the...
Extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae clinical isolates in a Senegalese teaching hospital: A cross sectional study
November 2017
Extended spectrum β-lactamase (ESBL) and carbapenemase-producing Enterobacteriaceae (CPE) have been increasingly reported worldwide. The objective of this study is to determine the prevalence of these multidrug-resistant strains in a major university teaching hospital in Dakar, Senegal. A total of 1205 Enterobacteriaceae stains were tested for ESBL and carbapenemase production. Antibiotics susceptibility test was...
A novel groel gene from the endosymbiont of beet leafhopper, Candidatus Sulcia muelleri
November 2017
Curtoviruses are transmitted by the beet leafhopper Circulifer tenellus, in a circulative (non-propagative) manner. Curtoviruses are phloem-limited and are acquired by the vector during feeding. Sap-feeding insects harbor endosymbionts which can help provide essential nutrients required for the insects’ survival. Candidatus Sulcia muelleri is an endosymbiont present in the beet leafhopper identified during this...
Prevalence and antimicrobial susceptibility of Salmonella species from lactating cows in dairy farm of Bahir Dar Town, Ethiopia
November 2017
Salmonella is the most important disease causing bacteria in persons as well as in animals. Antimicrobial-resistant Salmonella can be transmitted from animals to humans through consumption of contaminated food and food products. Thus, this study was conducted to determine the prevalence and antimicrobial susceptibility of Salmonella species from lactating cows in dairy farms of Bahir Dar town. A cross-sectional study...
Pityriasis versicolor profiles among outpatients of the Dermatology Department of the Teaching Hospital of Yopougon Abidjan, Cote d’Ivoire
November 2017
This cross-sectional and descriptive study was carried out to determine the socio-demographic, and clinical profiles of mycologically diagnosed pityriasis versicolor (PV) among outpatients of the Department of Dermatology, Teaching Hospital of Yopougon (Abidjan, Côte d’Ivoire) between July, 2012 and February, 2013. Among 1578 patients screened for various consultations, 200 presented with lesions suggestive...
In vitro biocontrol and biofertilization features study of a Bacillus amyloliquefaciens (4RH) strain isolated from a hot spring soil in Algeria
November 2017
Several Bacillus strains were isolated from “Ouled Yelass” hot spring soil, located in Setif city (Eastern Algeria). Three isolates of them coded (4RH), (14 RH), and (Set-oxy), were screened for their ability to inhibit the growth of some phytopathogenic fungi, such as Fusarium oxysporium, Botrytis cinerea, Aspergillus niger, Cladosporium cucumerinium and Alternaria alternata. The molecular identification of...
Antibiotic susceptibility pattern of bacterial isolates in children with otitis media in Zamfara, North-Western Nigeria
November 2017
Otitis media has been reported as a common childhood disease and is associated with multiple microbial pathogens within the middle ear. Though the causative agents of this infection have largely remained the same over the years, current literature reviews show that the antibiotic susceptibility pattern has changed considerably. This study aimed to identify bacterial isolates and determine their antibiotic susceptibility...
Isolation and characterization of bacteria associated with yolk sac infection (Omphalitis) in chicken from three hatcheries in Bishoftu, Ethiopia
November 2017
A study was conducted from November 2014 to June 2015 at three hatcheries designated as A, D and E in Bishoftu Town, Ethiopia, to isolate and identify bacteria associated with yolk sac infection and to determine antimicrobial susceptibility profile of the predominant isolates. A total of 385 Lohmann and Koekoek breed, 1 to 7 days old chicks suffering from yolk sac infection were examined from three different hatcheries...
Effect of rates and sources of soluble phosphorus on the behavior of cowpea plants inoculated with rhizobacteria from soils of the north region of Brazil
November 2017
Cowpea can obtain N through biological nitrogen fixation (BNF) through symbiosis with rhizobacteria. However, nodulation and BNF are influenced by edaphoclimatic factors that may bring about benefits or damages to the process, and the availability of nutrients is among the main factors that affect BNF and phosphorus (P). Thus, the present study aimed to determine the effectiveness of doses and sources of soluble P on...
Microbial quality of retail raw meat in administrative towns of Gojjam area North-West Ethiopia with special reference of Gram positive cocci species
November 2017
This study was conducted to determine the bacteriological quality of retail raw meat. Twenty one raw meat samples were randomly collected from four administrative towns: Bure, Debre-Markos, Dejen and Fenoteselam meat-stalls of Gojjam area. Samples were collected and transported kept in cold sterile screw cap bottles with ice contained icebox. It was cultured on plate count agar and mannitol salt agar plates for...
Microbial production of textile grade pigments
November 2017
Biological pigments or biochromes are substances produced by living organisms and have a color resulting from selective color absorption. A total of 37 isolates of Actinomycetes and 2 bacteria were isolated from 26 different soil samples collected from different habitats of Kerala. The isolates were screened for pigment production. The effect of pigment production by the strains on various carbon and nitrogen...
Biodegradation of petroleum hydrocarbon by Micrococcus sp. in form of wax ball
November 2017
Hydrocarbon containing compounds like diesel, crude oil and petrol cause environmental pollution. Hydrocarbon contaminated sites contain large amount of microbial community adapted for utilizing these compound as a source of carbon and other metabolic activities. A study was conducted in order to isolate the micro-organism from hydrocarbon contaminated sites for petroleum degradation. The soil sample was collected from...
Effect of surfactants on lignin modifying enzyme activity of Ganoderma species KX879638 and Lentinus species KY006984 under solid state fermentation
November 2017
Lignin modifying enzyme (LME) activity [laccase, lignin peroxidase (LiP) and manganese peroxidase (MnP)] was studied in two indigenously isolated white rot fungi, Ganoderma species KX879638 and Lentinus species KY006984 under solid state fermentation (SSF) of wheat bran and saw dust supplemented with various non-ionic polysorbate surfactants (Tweens) and Triton X-100. Both fungal species proved to be highly ligninolytic...
Production of antimicrobial agents by Bacillus spp. isolated from Al-Khor coast soils, Qatar
November 2017
Bacilli are Gram-positive, sporulating bacteria that can be found in diverse habitats, but mostly in soil. Many recent studies showed the importance of their antimicrobial agents that mainly target other Gram-positive bacteria. This study was conducted to assess the antibacterial activity of Al-Khor coastal soil Bacillus strains against four bacterial strains (Staphylococcus aureus, Staphylococcus epidermis, Escherichia...
Milk ring, rose bengal tests and conventional PCR based detection of Brucella abortus infected dairy cattle in Bangladesh
October 2017
The objective of this study was to detect dairy cattle infected with Brucella abortus from Jamalpur, Rangpur districts and Central Cattle Breeding and Dairy Farm (CCBDF), Savar, Dhaka in Bangladesh. Both milk and serum samples of 510 dairy cattle were initially screened by milk ring test (MRT) and Rose Bengal Test (RBT). Twelve samples positive in both MRT and RBT were further confirmed by conventional polymerase chain...
Diversity and antimicrobial resistance of Salmonella strains isolated from different sources in Burkina Faso
October 2017
Epidemiologic and traceback evidence of Salmonella infection is not much in many developing countries including Burkina Faso. This study investigates the antimicrobial resistance and distribution of Salmonella serotypes isolated from diverse sources in Burkina Faso. 615 Salmonella serotypes isolated from beef meat, poultry carcasses, poultry, swine, cattle, hedgehog, fish, salad, channel and humans from 2009 to 2011...
Comparative evaluation of the antimicrobial profile of Moringa leaf and seed oil extracts against resistant strains of wound pathogens in orthopedic hospitals
October 2017
The antimicrobial profile of oil extracts from Moringa oleifera leaves and seeds on orthopaedic wound pathogens was tested and compared with the antimicrobial activity of some antibiotics. The pathogens were characterized using biochemical and morphological tests. Antimicrobial susceptibility test was done on the pathogens using paper discs diffusion method. Plasmid curing was done on the isolates that showed resistance...
Study of kefir biofilm associated with hydroethanolic extract of Euterpe oleracea Mart. (açai)
October 2017
Kefir is a microbial complex capable of producing biofilms. This study aims to obtain biofilms associated with hydroethanolic extract of Euterpe oleracea Mart. (BEHEEo) as fixing method of recovery anthocyanins in kefir biofilm. Thus, BEHEEo formation kinetics was made at different concentrations of aqueous extract of E. oleracea, of kefir grains (KG) and of brown sugar (BS). Thereafter, macro and microelements of...
Bacterial and fungal etiologies of meningeal irritation syndrome in an emergency department
October 2017
Meningeal irritation syndrome denotes to a range symptoms that require emergency management. Clinically, it is an expression of an irritation of the meninges combining with headache, fever, neck stiffness, altered consciousness and vomiting. The aim of this prospective study was to contribute to the management of medical emergencies. Cerebrospinal fluid (CSF) samples recovered from patients presenting signs of meningeal...
Endophytic bacteria isolated from ipê mirim (Tecoma stans Bignoniaceae) and its application for plant growth promotion
October 2017
Endophytes are usually protected from soil environment competitiveness and stress. However, the presence of heavy metals can negatively affect the structure and diversity of endophyte communities. The aim of the present study was evaluate the diversity of endophytic bacteria from ipê mirim (Tecoma stans Bignoniaceae) grown in an area of Atlantic rainforest contaminated with metals and evaluate the ability of these...
Cholesterol reduction in vitro by novel probiotic lactic acid bacterial strains of Enterococcus isolated from healthy infants’ stool
October 2017
The present study aimed to isolate and evaluate the potentiality of some probiotic lactic acid bacteria from infants stool to degrade and reduce cholesterol level in fermentation medium. Out of 50 lactic acid bacterial isolates recovered from healthy infants (3 to 18 months old) stool, two bacterial isolates W7 and Y1 showed highest cholesterol reduction percentage (68.38 and 71.6%, respectively). The cholesterol...
Effect of EDTA on biofilm formation and antibiotic susceptibility of multidrug resistant uropathogenic Escherichia coli clinical isolates in Egypt
October 2017
Urinary tract infections are mainly caused by uropathogenic Escherichia coli (UPEC). Biofilm-producer UPEC tends to have a high level of resistance to antibiotics and this leads to recurrent episodes of urinary tract infections. The study tested the effect of a non-antibiotic adjuvant, ethylenediaminetetraacetic acid (EDTA) on the bacterial susceptibility to antibiotics and biofilm formation by multidrug resistant (MDR)...
Effect of phosphorus fertilization on arbuscular mycorrhizal fungi in the Bambara groundnut rhizosphere
October 2017
Tropical soils are highly leached, abundantly clayey and strongly acid, resulting in low mineral availability and especially phosphorus (P). The plants of this region must establish symbiotic relationships enabling them to strengthen their hydromineral nutrition. The aim of this work was to study the effect of P fertilizer application on the diversity of arbuscular mycorhizal fungi (AMF) under the rhizosphere of three...
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