African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002

AJB Articles in press

Article in Press

USE OF PECTIN IN FRUIT CAGAITA (EUGENIA DYSENTERICA DC.) TO EVALUATE POSTHARVEST QUALITY

This study aimed to evaluate the postharvest conservation of cagaita fruits coated with pectin-based coating. Fruits were subjected to four treatments: control (uncoated fruit), 2, 4 and 6% (v/v) pectin coating and stored in polystyrene trays at 12°C for 30 days. The parameters analyzed were: weight loss, titratable acidity, vitamin C, soluble solids, peak strain, color and scanning electron microscopy (SEM) at 0, 10, 20...

Author(s):GEOVANA ROCHA PLACIDO

Article in Press

Modern Diagnostic Tests for Veterinary Viral Diseases

The diagnosis of viral diseases in animals and human is a challenge as viral infection appears in syndrome varying from acute to chronic, mild to fulminating or fatal to latent disease situation. The veterinary and medical professionals providing therapeutics get completely dependent on the diagnostic laboratories for confirmation of viral diseases. Therefore, availability of rapid, reliable and sensitive diagnostic assays...

Author(s):RAJEEV SINGH

Article in Press

Antioxidant enzymes as a biomarker apt for the appraisal of heavy metals exposure in Enterobacter cloacae and Bacillus subtilis

The effects of exposure to heavy metals under laboratory conditions were studied in the bacteria AB6 and AB4 isolated from a polluted site. 16S rRNA sequencing divulged that the AB6 and AB4 were phylogenetically related to Enterobacter cloacae and Bacillus subtilis. The examined biological responses includes concentration of protein, the activity of Catalase (CAT), superoxide dismutase (SOD), glutathione peroxidise (GR)...

Author(s):SURIYA. J

Article in Press

Production of bacterial biodegradable plastic by low coast agricultural waste material

Abstract Polyhydroxybutyrates (PHBs) are polyesters that accumulate as inclusions in a wide variety of bacteria. Because of their inherent biodegradability, PHBs are considered as good candidates for production of biodegradable plastic. In the present study, we aimed to study the production of PHBs by bacterial strain isolated from divers’ sources using inexpensive substrates. Fifty bacterial strains isolated from...

Author(s):ANTENEH GETACHEW

Article in Press

Effect of chitosan stabilized biogenic silver nanoparticles incorporated levofloxacin (CS-AgNps-LF) nano drug conjugate coating on the mechanical properties and anti bacterial activity of wound dressing

The high distribution of resistance of a wide range of microorganisms to the diverse range of antimicrobial agents is attracting a great deal of attention. Development of effective and safe drugs based on nanoprinciples are now extensively utilized to fight against pathogenic microorganism.Among the various route ,infection through cross contamination on various objects including wound dressing causes severe health risks...

Author(s):Namasivayam Karthick Raja

Article in Press

SCREENING OF EXTRACELLULAR HYDROLYTIC ENZYMES PRODUCTION BY ASCIDIANS ASSOCIATED BACTERIA FROM TUTICORIN, SOUTHEAST COAST OF INDIA

The aim of this study was to isolate and characterize the ascidians associated bacteria with extracellular enzymes from Tuticorin, Southeast coast of India. Totally 21 culturable heterotrophic bacteria were isolated from the three species of ascidians (Polyclinum glabrum, Microcosmus exasperates and Phallusia arabica). Among them, three isolates (GA CAS11, GA CAS12 and GA CAS13) showed extracellular enzymes production. The...

Author(s):ANANTHAN G.

Article in Press

Comparative Survival Strategy of Vibrio cholerae and Vibrio parahaemolyticus in Different Stress Conditions and Recovery of Viable But Non-Culturable (VBNC) Cells

Entry of viable but non-culturable (VBNC) state in human pathogen Vibrio cholerae O139, O1 Ogawa and V. parahaemolyticus O3:K6 serotypes under different medium at different temperatures were studied. The results revealed that V. parahaemolyticus survived well even in frozen condition for more than 110 days, whereas V. cholerae O139 and O1 Ogawa were able to survive only up to 17 and 4 days respectively at -20°C. The plate...

Author(s):Athmanathan Baskaran

Article in Press

GENETIC DIVERSITY AND POPULATION STRUCTURE OF ELITE MAIZE INBRED LINES OF ZAMBIA USING SNP MARKERS

Maize is the most important source of energy in Zambia and it is the most commonly grown crop. Over the years, the Zambian public maize breeding programme has a collection of about 800 maize inbred lines for hybrid breeding. However, these inbred lines have not been characterised. Therefore, a study was conducted on a sample of 153 inbred lines with the objective of assessing the genetic diversity and determining the...

Author(s):Chanda Richard

Article in Press

Acute Toxicity Impacts and Heavy Metals Bioaccumulation in the Head-Vertebrae and Flesh of Clarias gariepinus (Burchell, 1822) Exposed to Cassava Waste Waters

The Acute toxicity of cassava waste water were investigated on Clarias gariepinus using a renewable static bioassay with emphasis on comparing the bioaccumulation pattern of Zinc, Manganese, Copper, Cadmium and Lead in the head-vertebrae part and flesh. Alteration in swimming activity and feeding, respiratory disturbance, anaemic morphology, skin lesion and death were observed in the exposed fishes and these varied greatly...

Author(s):TEMIDAYO EBENEZER OLAGUNJU

Article in Press

BARU (Dypterix alata Vog) MESOCARP FLOUR: AN ALTERNATIVE FOR THE PRODUCTION OF BIOETHANOL

The Baru (Dypterix alata Vog) belongs to the Leguminosae family; it is composed of mesocarp. endocarp. and almond. The mesocarp represents 5% of the whole fruit and consists of up to 38% starch and may be used for the ethanol production since this biofuel is produced by the conversion of starch. However. for such conversion. the starch should be hydrolyzed and thus converted to sugars. This hydrolysis may occur by acidic...

Author(s):PAULO CLEBER MENDONçA TEIXEIRA, Abraham Damian Giraldo Zuniga

Article in Press

Optimization of Cellulase production from Bacillus species

Cellulose is most abundant biological component on terrestrial and aquatic ecosystem. Being the largest component of terrestrial and aquatic ecosystem conversion of this complex carbohydrate to a simpler sugar is having brighter future. The conversion of cellulose to one of the simplest form of sugar which is glucose is having bright future. Some and bacteria are capable of these conversion so in this experiment our main...

Author(s):ABINASH SHARMA

Article in Press

Improvement Of Nutritional, Sanitary And Organoleptic Qualities Of Liquid Zoom-Koom And Instant Flour Zoom-Koom Using Lactobacillus Fermentum Starter Culture

Traditional fermented beverages occupy an important place in the diet of populations in Burkina Faso. Zoom-koom in particular remains one of the most consumed cereal-based drinks in cities like Ouagadougou. The drink zoom-koom is consumed primarily in the liquid form and to short scale in the reconstituted form called instant flour zoom-koom. Being able to diversify zoom-koom taste and aroma and to improve the nutritional...

Author(s):Soma MAAR, Kaboré D, Tankoano A, Compaoré CS, Parkouda C, Toguyeni A, Sawadogo-Lingani H

Article in Press

Characterization of three full Iris yellow spot orthotospovirus genes of a garlic-infecting isolate from Zimbabwe using Next-generation sequencing technology

Iris yellow spot orthotospovirus (IYSV) is an important pathogen of Allium species worldwide. It has a tripartite genome consisting of the large (L), medium (M) and small (S) RNA segments. Despite its worldwide distribution, very few complete gene and genome sequences are available in public databases. The aim of this study was to obtain full gene sequences of a garlic-infecting IYSV isolate by Next-generation sequencing...

Author(s):Charles Karavina, Jacques Davy Ibaba and Augustine Gubba

Article in Press

Nutritional value and biosafety of Dioscorea bulbifera L. (air potato) fermented with Pleurotus ostreatus and Calocybe indica

The proximate, minerals, antinutrient and amino acid contents of the unfermented air potato (UAP), fermented air potato (APF), fermented air potato with Pleurotus ostreatus (APP) and fermented air potato with Calocybe indica (APC) were carried out using standard methods. The biosafety of the samples was also determined using Wistar rats. APP has the highest crude fiber (11.61%) and protein content (20.44%). APF has the...

Author(s):Tolulope Bolaniran, Clement Olusola Ogidi and Bamidele Juliet Akinyele

Article in Press

Studies on some major yield responsive genes in selected rice (Oryza species) cultivars grown in Nigeria using candidate gene SSR-based markers approach

This study was aimed at determining the presence of five major yield responsive genes shown to be among key determinants of rice yield in ten rice cultivars grown in Nigeria using candidate gene SSRs markers (Cg-SSRs). DNAs were extracted from young leaf samples using optimized cetyltrimethylammonium bromide (CTAB)-extraction method. An established gene sequences for high yielding rice Nipponbare cultivar for the five...

Author(s):Zaharaddin Halilu, Rabi'u Aliyu Umar, Riskuwa Arabu Shehu, Adamu Aliyu Aliero, Aliyu Abdullahi Turaki, Ahmed Hamza Balarabe, Abubakar Muhammad Gumi and Sha'aya'u Shehu

Article in Press

An Investigation of Meristem Culture in Anchote [Coccinia abyssinica (Lam.) Cong.] Under the Influence of Plant Growth Regulators and Media Strength

Considering the potential benefit of meristem culture in the mass production of quality propagation materials, an experiment was conducted to investigate meristem culture in KUWE (G1) and 223098 (G2) genotypes of anchote. Consentration levels of 6-Benzylamimopurine (BAP) with and without Gibberellic Acid (GA3) and Naphthalene Acetic Acid (NAA) for shoot induction while BAP and Kinetin (Kn) for shoot multiplication were...

Author(s):Solomon Abate Sisay, Firew Mekbib and Endale Gebre

Article in Press

Genetic diversity analysis of Ethiopian elite chickpea (Cicer arietinum L.) varieties based on agronomic characteristics

The objective of this study was to assess the morphological diversity of the elite chickpea (Cicer arietinum L.) varieties of Ethiopia. Nineteen elite varieties of chickpea in Ethiopia were used to analyze the means and components of variability (genetic, phenotypic and environmental), and interrelationships (genetic and phenotypic) for yield and various other yield components. Such nineteen varieties were planted by the...

Author(s):Zerihun Jida and Shiferaw Alemu

Article in Press

Changes in some behavioral, hematological and biochemical indices of air-breathing Clarias gariepinus [BUCHELL, 1822] exposed to pharmaceutical effluent

This work aimed at evaluating the behavioral, biochemical and hematological effects of pharmaceutical effluent in laboratory population of Clarias gariepinus using a static renewal bioassay system. Fish specimens were collected and exposed to five (0.04, 0.06, 0.08, 0.10 and 0.12 mgL-1) sublethal concentrations of the effluent including a control experiment. Different dose dependent behavioral responses such as erratic...

Author(s):Ogundiran, M. A. and Ayandiran, T. A.

Article in Press

Evaluation of the physical properties of leathers tanned with Plectranthus barbatus Andrews extracts

This study was designed to assess the potential use of Plectranthus barbatus Andrews, a plant growing locally in Kenya as a vegetable tanning agent. The aim was a deliberate attempt to adopt green chemistry and provide cheap locally available eco-friendly tanning materials. Anova statistical test (α

Author(s):Dennis Kimaiga Obiero , Arthur Steven Onyuka and Alvin Asava Sasia

Article in Press

Fermentation of tigernut (Cyperus esculentus Linn) tuber must using yeasts (Saccharomyces cerevisiae) and its potential for wine making

There is lack of information on tigernut wine production especially dealing with the suitability of the yellow varieties of tigernut for wine production. This research was undertaken to investigate the suitability of producing tigernut wine from tigernut tuber. To prepare the wine, the tuber was washed and must extracted immediately after crushing, then batch fermentation of the inoculated must was carried out by...

Author(s):EKEANYANWU, Raphael Chukwuma, UGWUEGBULAM, Peace Chisom and OPARAIHEOMA, Immaculeta Chisom

Article in Press

Potential applications of enzymes on the extraction of vitexin from dried Mas Cotek leaves

Mas Cotek or Ficus deltoidea var. deltoidea leaves extracts contain various beneficial properties such as antidiabetic, antihypoglycemic and antioxidant activities, which can be enzymatically extracted in water. Vitexin is one of the antioxidants and remarkable compound of flavone C-glycosides found in Mas Cotek leaves. Cellulase is one of the essential enzymes applied to hydrolyze cellulose into fermentable sugars and...

Author(s):Nasma Alim, Aishath Naila, Azilah Ajit, A. Z. Sulaiman and M. N. Che Isa

Article in Press

Physicochemical factors that influence the stability and bioavailability of lipid based drug delivery systems (LBDDS): A bird eye view

Lipid excipients are abundantly distributed in nature, biodegradable and are generally regarded as safe (GRAS). It enhances the absorption of lipophilic drugs and therefore, has evoked a boost in application of lipids as carrier for most lipophilic drugs. Lipid based drug delivery systems (LBDDS) have feasibility of different delivery routes including oral, parenteral, pulmonary, topical, ocular, intravaginal and...

Author(s):Salome A. Chime and Anthony A. Attama

Article in Press

Genetic variability of improved maize varieties (Zea mays L.) for acidic soil tolerance under contrasting environments in Assosa, Ethiopia

Maize is the second most widely grown food cereal crop cultivated in the world and consumed in various form of as part of diets of human and animals. However, its production and productivity is affected by biotic and abiotic stresses among which, soil acidity is the key factor. This field experiment was conducted to estimate the genetic variability of maize for yield and yield related traits, and determine the association...

Author(s):Yaregal Damite and Firew Mekbebe

Article in Press

Evaluation of in vitro antioxidant and immunomodulatory activities of polysaccharide fractions of Khaya grandifoliola C.D.C (Welw) stem bark and Cryptolepis sanguinolenta (Lindl.) Schltr leaves

Antioxidant and immune modulatory activities of polysaccharides fractions isolated from Khaya grandifoliola stem bark and Cryptolepis sanguinolenta leaves were analyzed. Water-soluble total polysaccharides (TP) were extracted and precipitated in alcohol. Pectic (PF) and hemicellulosic fractions (HF) of cell walls were obtained using ammonium oxalate and potassium hydroxide. TP of K. grandifoliola and C. sanguinolenta...

Author(s):Francine Mediesse Kengne, Marius Trésor Sipping Kemegne, Mersmine Flore Kouamo Mangoua, Kenji Obadiah, Samuel Tassi Yunga, Wilfried Fon Mbacham, Rose Gana Fomban Leke and Thaddée Boudjeko,

Article in Press

Analysis of production of biogas from peel of fruits and leaf of vegetable with cow dung as inoculants under anaerobic digestion

Biogas is a renewable source of energy that belongs to the category of biofuels. Anaerobic condition typically refers to a gas produced by the biological breakdown of organic matter in the absence of oxygen. Fruit and vegetable wastes (FVW) were evaluated from feed stalk materials for biogas production under anaerobic digestion. The biogas production and methane content of fruit and vegetable wastes digestion were...

Author(s):Massreshaw Assnakew Abebe

Article in Press

Biochemical characterization of a cellulase-free xylanase obtained from a newly isolated fungus, Mucor Mucedo, grown under optimized conditions

The study of the isolation, screening and identification of a xylanase-producing fungus, purification and characterization of xylanase was undertaken in order to produce the enzyme from a cheap source, saw dust, for possible industrial use. Eight fungal strains were isolated and identified from saw-dust out of which Mucor mucedo had the maximum xylanase production and it was selected as the best xylanase-producing strain....

Author(s):Adejumo, Adekunle Testimony, Ademakinwa, Adedeji Nelson, Ayinla, Zainab Adenike, Adejumo, Ayooade Lateef and Agboola, Femi Kayode

Article in Press

Phylogenetic analysis of bluetongue virus serotype16 based on genome segment 5 (encoding NS1)

Bluetongue virus (BTV) is the ‘type’ species of the genus Orbivirus within the family Reoviridae. The BTV genome is composed of ten linear segments of double-stranded RNA (dsRNA), each of which codes for at least one of ten distinct viral proteins. Phylogenetic analysis based on VP2, VP3, VP5, VP7 and NS3 gene has been advocated by different researchers around the world. However, very little information about the...

Author(s):Molalegne Bitew, Sukdeb Nandi, Chintu Ravishankar and Asit Sharma

Article in Press

The nutrient composition of three indigenous fruits in Botswana

Indigenous fruits play important roles in food security in rural and urban areas of many African countries. Of several indigenous grown in African countries, three fruits (Azanza garckeana, Grewia flava and Ximenia caffra) were selected for the study. The edible portions were analyzed for vitamin C, titratable acidity, dry matter, ash, crude protein, acid detergent fibre (ADF), acid detergent lignin (ADL), neutral...

Author(s):Olekile Tibe and Joel L. Seolwane

Article in Press

Genetic diversity of Aegilops tauschii Coss. among Chinese populations using single sequence repeat (SSR) markers and morphological traits

Aegilops tauschii Coss. (2n = 2x= 14, DD) is a problematic weed and contributes to the D genome in common wheat. Genetic diversity among 40 populations of China was evaluated by microsatellites marker and morphological traits. For dry biomass weight, there was variation among the populations, while for plant height, there was a high variation (15.8%) among the populations. We determined 27 alleles by using eight primers....

Author(s):Adeel Abbas, Haiyan Yu, Hailan Cui, Huilin Yu and Xiangju Li

Article in Press

Investigation of haematological changes following Kolaviron intervention in Nevirapine therapy: An exerimental animal model

This study evaluated the haematotoxic effects of Nevirapine and the role of Kolaviron, a potent antioxidant from Garcinia kola as an adjuvant. Forty eight adult male Sprague-Dawley rats were used in this study. The animals were divided into eight groups, A-H (with 6 rats per group). Group A animals received normal saline as the control; Group B were given Nevirapine (1.54 mg/kg); Group C received Kolaviron (200 mg/kg);...

Author(s):Ajayi Sunday A., Azu Onyemaechi O., Naidu Edwin C. S., Naidu Jesse S., Komolafe Omobola A. and Ofusori David A.

Article in Press

Biocontrol activity of chitinase from marine sponge Callyspongia diffusa associated Pseudomonas fluorescens BCPBMS-1

Marine sponge Callyspongia diffusa associated with Pseudomonas fluorescens BCPBMS-1 was isolated from Madapam Coast, Tamil Nadu, India and screened for chitinase production. Chitinase was tested for its biocontrol activity against phytopathogenic fungi. Chitinase is an enzyme capable of hydrolyzing chitin, an insoluble polysaccharide present in the cell wall of higher fungi, insect gut walls and nematodes. Chitinase was...

Author(s):V. Vasanthabharathi and S. Jayalakshmi

Article in Press

Isolation of cellulase producing Aspergillus unguis UCSC324 and characterization of cellulase

Bioengineering of cellulolytic enzymes with enhanced catalytic efficiency and thermostability is important in the commercialization processes. This study describes the isolation of efficient cellulase producing fungi and determination of the kinetic properties of the crude cellulase. Among the fungal strains isolated from cow dung, hot rice water, water used in autoclave and decaying coconut wood, the strains growing on...

Author(s):Mohanappriya Shanmugarajah & Ranganathan Kapilan

Article in Press

Regeneration of Washington navel sweet orange (Citrus sinensis L. Osb.) by in vitro organogenesis from floral and vegetative explants

For viral sanitation strategy in Tunisia, citrus accessions from Washington navel (Citrus sinensis L. Osb.) variety were investigated for regeneration throughout direct and indirect in vitro organogenesis. In this study, either floral (style/stigma and ovaries) or vegetative organs (leaves, cotyledons, epicotyls and hypocotyls) were cultured into Murashige and Skoog (MS) culture media that were supplied with five different...

Author(s):Nebiha METOUI, Fethia DHAOUADI and Dorsaf YAHIAOUI

Article in Press

Isolation of Ursolic Acid from the Leaves of Ocimum lamiifolium collected from Addis Ababa Area, Ethiopia

Ocimum lamiifolium is a well known medicinal plant in Ethiopia. Fresh leaves of the plant are squeezed and sniffed to treat colds and coughs and as an eye rinse for eye infections, while crushed leaves are used to arrest nose bleeding. In this study phytochemical investigation on the methanol extracts of this plant collected from Addis Ketema subcity, Addis Ababa resulted in the isolation of a triterpene, namely U

Author(s):Girma Birhanu

Article in Press

Thiourea medium supplementation improves salinity tolerance of hybrid maize: Evidence from whole plant and cellular level studies

These experiments were conducted to evaluate the role of thiourea on accumulation of osmoprotectants and antioxidants in the salt stressed maize roots and callus of maize hybrids. Medium supplementation of 400 µM thiourea was applied on two maize hybrids; 33M15 and DK6789. The maize hybrid 33M15 performed better than DK6789. Fifteen days older plants and immature embryo were subjected to medium supplemented with thiourea...

Author(s):Tayyaba Sanaullah, Ali Noman, Muhammad Aqeel, Noreen Khalid, Abdul Wahid and Bushra Sadia

Article in Press

Zinc biofortification of wheat as influenced by zinc fertilization

Zinc (Zn) fertilization is an effective agronomic tool for Zn biofortification of wheat to overcome Zn deficiency in humans. Zinc deficiency in humans caused by inadequate dietary intake is a global nutritional problem. Biofortification of wheat with Zn is, therefore, an important challenge and a high-priority research work. In the present study, a field experiment was conducted to explore the impact of soil and foliar Zn...

Author(s):Mohammad Delwar Hossain, Motarab Hossain, Ahmad Khairul Hassan and Ahmad Ainuddin Nuruddin

Article in Press

Organic matter and N removal performance of a recirculating aquaculture system (RAS) for rainbow trout culture with aerobic-anaerobic reactor, three phase airlift fluidized bed reactor and filtration units

The performance of an aerobic three phase fluidized bed reactor for the treatment of surface water in the culture tanks effluent, and an anoxic-anaerobic reactor for the bottom effluent of the rearing tanks treatment is show in this study. The main objective of the study was to evaluate the organic matter and nitrogen removal efficiency in a recirculating aquaculture system (RAS) for intensive rainbow trout laboratory...

Author(s):Gloria Cárdenas Calvachi and Iván Sánchez Ortiz

Article in Press

Genetic dissection of quantitative trait loci (QTL) associated with agronomic traits in an interspecific rice population evaluated under drought and irrigated environment

Numerous studies have been conducted in rice to map quantitative trait loci (QTLs) associated with different agronomic traits. However, only a few of these studies have used interspecific mapping populations. Here, QTLs associated with tiller numbers, flowering time, plant height, and grain yield in an interspecific rice population were mapped. The population was evaluated under managed water-stressed (drought) and optimal...

Author(s):Marie Noelle Ndjiondjop, Peterson W. Wambugu, Mamadou Cissoko, and Kassa Semagn

Article in Press

Comparison of meristem culture and heat therapy to clean garlic (Allium sativum L.) infecting virus in Ethiopia

This experiment was aimed to detect garlic infecting virus for three improved garlic varieties (Bishoftu Nech, Tseday and Kuriftu) collected from different garlic production sites of Ethiopia. Garlic cloves were planted in screen house at Biosciences Eastern and Central Africa-International Livestock Research Institute Hub Nairobi, Kenya in 2014. Reverse Transcription polymerase chain reaction (*RT-PCR) technique was...

Author(s):Abel Debebe

Article in Press

In vitro propagation of the wild medicinal plant, caper (Capparis spinosa L.)

Micropropagation of caper (Capparis spinosa L.) was done using wild leaves from Libya. High percentage (95%) of callus induction was obtained from Murashige and Skoog (MS) medium supplemented with 1.2 mg l-1 2,4-D + maltose. The effect of various concentrations (0.0, 0.5. 1.0 and 2.0 mg l-1) of cytokines (BAP, 6-γ-γ-[Dimethylallylamino]-purine 2ip, Kin and Zea) supplemented with MS medium was evaluated for callus...

Author(s):Adel M. Elmaghrabi, Elmundr Abughnia and Salem Hamoud

Article in Press

Role of Toll-like receptor 4 in regulating nucleotide-binding oligomerization domain2 (NOD2)-mediated inflammatory responses through Aspergillus fumigatus conidia stimulation in RAW264.7 macrophages

Nucleotide-binding oligomerization domain2 (NOD2) of the NOD-like receptors (NLRs) and Toll-like receptor4 (TLR4) of the TLRs are major pattern-recognition receptors. They sense different microbial pathogens and play important roles in innate immunity. Current in vivo and in vitro studies have shown that TLR4 and NOD2 act in anti-Aspergillus fumigatus infection. Herein, the cross-interaction between membrane-bound TLR4 and...

Author(s):MA Guang-qiang, WU Su-fang, LUO Hong-dan, XIE Wei-hua, LONG Kai, SU Ming-sheng and XIE Xiao-mei

Article in Press

The oxidative stability of linolenic fatty acid in farmed rainbow trout (Oncorhynchus mykiss) under different storage conditions in Isfahan City of Iran

The aim of this study was to investigate the effect of different storage conditions in market (ice box, glassy refrigerator, normal refrigerator without light (natural and electric power) and freezer) on alpha linolenic acid (ALA), a nutritional value of rainbow trout (Oncorhynchus mykiss). To investigate the effect of storage duration on fatty acid quality, samples stored after 1, 3 and 5 days in refrigerator and those...

Author(s):Lakzadeh, L., Mirmohammadi, M., Amouheidari, M. and Bavarian, A.

Article in Press

pression, Purification and On-Column Refolding of Recombinant S-layer Protein from L. acidophilus ATCC 4356

One of the most significant challenges in biotechnology is technological utilization of self-assembled systems. Surface (S-) layer technology is advanced due to the intrinsic property of subunits to reassemble into two-dimensional arrays. Lactobacillus acidophilus ATCC 4356 composed of S-layer protein (SlpA) subunits which are organized into regular arrays. Herein, S-layer gene (slpA) of L. acidophilus was cloned in...

Author(s):Elaheh Sadat Hosseini , Rouha Kasra Kermanshahi , Saman Hosseinkhani and Mahboobeh Nazari

Article in Press

Scanning electron microscope studies of pollen of herbaceous species of Amritsar, North-Western Punjab, India

Palynological studies in combination with conventional morphology have a significant role in solving problems relating to plant systematics. Scanning electron microscope (SEM) is a useful tool for palynological studies with increased accuracy and precision. The pollen size, shape and details of sculpturing of the exine are of taxonomic value. The main objective of the present study was to provide a detailed morphological...

Author(s):Harpreet Kaur and Avinash Nagpal

Article in Press

Merzouga Sand Dunes Actinobacteria: antimicrobial potential and taxonomic diversity

Merzouga sand dunes were among the Moroccan extreme environments. Actinobacteria from this under investigated desert habitat were isolated and their antimicrobial activity was evaluated. One hundred sixty three Actinobacteria isolates were isolated from sand collected from several dunes and screened for their antibacterial and antifungal activities. To test the antimicrobial effect of isolates, four microorganisms known as...

Author(s):Lahcen Ouchari, Amal Boukeskasse, Brahim Bouizgarne and Yedir Ouhdouch

Article in Press

Protocol optimization of in vitro propagation of an endangered endemic Ethiopia medicinal plant, Echinops kebericho (Mesfin), using shoot tip

Echinops kebericho (Mesfin)isthe most important and critically endangered endemic Ethiopian medicinal plant. This plant is threatened due to over harvesting of its roots for medicinal purpose, and from seed viability and seed germination problem. The aim of this research was to build up an efficient in vitro propagation protocol for E. kebericho using shoot tip explants. Shoots excised from in vitro germinated seedlings...

Author(s):Muluken Enyew; Tileye Feyissa

Article in Press

Development of phage mediated quartz crystal microbalance (QCM) sensor for the detection of Escherichia coli, a food-borne pathogen

This study presents the development of phage mediated quartz crystal microbalance (QCM) sensor for the detection of Escherichia coli. Bacteriophage is very specific to host bacterial cell also known as parasite for bacteria. The Electrochemical Quartz Crystal Microbalance (EQCM) is a very sensitive device that measures a mass change per unit area by measuring the change in resonant frequency of a quartz crystal. A...

Author(s):Vipin Singh and Pranay Jain

Article in Press

Morphological diversity studies among sugarcane (Saccharum spp.) somaclones generated through callus culture

Callogenesis is one of the tools in sugarcane tissue culture for generating agronomically significant variation. Tissue culture derived variations are known as somaclonal variation. The study was carried out to investigate the effect of MS media supplemented with 1.0 mgL-1 of NAA (naphthalene acetic acid) and 2.0, 2.5, 3.0, 3.5 and 4.0 mgL-1 of 2,4-D (2,4-dichlorophenoxy acetic acid) on somaclonal variation in in vitro...

Author(s):Richard Wekesa, Justus M. Onguso, Bernard A. Nyende and Leonard S. Wamocho

Article in Press

Biodegradation of crude oil by Bacillus species isolated from different sources

The consequences of oil pollution to the environment and human health as a result of exploration activities, transportation and usage of petroleum compounds, has become a serious challenge to environmental scientists. This study evaluates the potential of using Bacillus subtilis in biodegradation of petroleum compounds. Ten Bacillus strains from non-petroleum contaminated sites were identified on the basis of microscopy...

Author(s):Mohammed Inuwa Ja’afaru, Chimezie Godwin Azuchukwuene and Aisha Sa’id Shittu

Article in Press

Analytical quantification of copper in frogs (Rana tigrina) found from various aquatic habitats

This study focused on the quantitative determination of Cu metal in various physical parts of frogs by using the Atomic Absorption Spectrometry technique). Cu was measured (2.72±0.77 µg/g) in kidney of Rana tigrina captured from industrial site and in R. tigrina captured from non-industrial site (28.10±27.02 µg/g). Similarly, 27.56±9.92 µg/g Cu was discovered in liver of R. tigrina captured from industrial site and...

Author(s):Muhammad Nasir, Tariq Mahmood Ansari, Ghulam Yasin, Aleem Ahmad Khan and Muhammad Shoaib

Page 2 of 3, showing 50 records out of 119 total, starting on record 51, ending on 100