African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002

AJB Articles in press

Article in Press

Psychrotrophic proteolytic bacteria and their characterization from landfill soil under temperate climatic conditions of Kashmir Himalaya

During cold season in Kashmir valley, the decomposition of municipal solid waste slows down due to slowing down of microbial activity as a result, the waste gets piled up and its management becomes a greater challenge. Psychrotrophic proteolytic bacteria play an important role in the degradation of protenaceous materials of the waste stream under low temperatures that produces nitrogen sources for rapid functioning of...

Author(s):Basharat Hamid, Arshid Jehangir and Zahoor Ahmad Baba

Article in Press

Holistic plant regeneration protocol from callus cultures of Salvia moorcroftiana, a medicinal herb

Salvia moorcroftiana is a medicinal herb of the family Lamiaceae. The objective of this study was to develop an in vitro protocol for the rapid propagation of this species. Organogenesis by means of callus culture was carried out on Murashige and Skoog medium by the transfer of callus from an induction medium supplemented with α-naphtalenacetic acid (NAA, 1.0 mg/L) to regeneration medium containing 6-benzyladenin (BA, 1.0...

Author(s):Tour Jan, Ali Hazrat ,Rashid Azam, Arshad Ali Mohammad Nisar, Nasrullah Khan and Muhammad Asif Nawaz

Article in Press

Genetic variability, heritability and genetic advance for green pod yield and yield related traits in chili (Capsicum annum L.) accessions in Ethiopia

A field experiment was conducted to study the genetic variability, heritability and genetic advance for 12 quantitative characters in chili (Capsicum annum L.) accessions in Ethiopia, during the off-season period of 2012/2013 under flood irrigation at the Adet Agricultural Research Centre field. The experiment was conducted using Randomized Complete Block Design with three replications and a total of 25 accessions and four...

Author(s):Tadesse Tilahun, Yohannes Petros and Yigzaw Dessalegn

Article in Press

Effects of temperature and time on oil extraction from some Nigerian indigenous fresh water microalgae species

This study determined the effects of temperature and time on oil extraction from indigenous freshwater microalgae: Dictyosphaerium, Chlorella, Desmodesmus and Cosmarium species, cultured in 15 2-L column photobioreactors (PBR) (three per species). Growth and specific growth rates for the mixotrophic>autotrophic cultivation of the species were determined. Oil was extracted from the dried microalgae species at temperatures...

Author(s):Felix Uzochukwu Asoiro, Wilfred Ifeanyi Okonkwo, Clement Onyeaghala Akubuo, Nkechinyere O. Nweze and Cosmas Ngozichukwu Anyanwu

Article in Press

Atomic absorption spectroscopic evaluation of some heavy metal contents in tomato (Lycopersicon esculentum Mill) fruit and Ribu River water used for irrigation at Fodugora Farm, Weliso Town, Ethiopia

The levels of some heavy metals in mature fruits of tomato (Lycopersicon esculentum Mill) grown at Fodugora Farm, Weliso Town (Ethiopia) with Ribu River effluent water, used for its irrigation, have been determined using flame atomic absorption spectroscopic (FAAS) technique. Whereas, the levels of Fe, Zn and Pb in tomato fruits were: 34.05, 4.05, and 1.32 mg/L respectively, their concentrations in the river effluent water...

Author(s):Gera Techane, O. P. Yadav and LokeshYadav

Article in Press

Genetic diversity assessment of yams (Dioscorea spp.) from Ethiopia using inter simple sequence repeat (ISSR) markers

The genetic diversity and relationships of 70 accessions of yam belonging to Dioscorea cayenensis/Dioscorea rotundata complex (55), Dioscorea bulbifera (13) and Dioscorea alata (2, as a reference) were assessed using six inter simple sequence repeat (ISSR) primers. DNA was extracted from a bulk of two plants per accession using a modified cetyl trimethyl ammonium bromide (CTAB) method. Six ISSR primers amplified 77...

Author(s):Kedra Mohammed Ousmael

Article in Press

Study of secondary metabolites produced by Aspergillus flavus and evaluation of the antibacterial and antifungal activity

The objective of this study was the analysis of the secondary metabolite products and evaluation of antibacterial activity of Aspergillus flavus. Bioactives are chemical compounds often referred to as secondary metabolites. Thirty one bioactive compounds were identified in the methanolic extract of A. flavus. The identification of bioactive chemical compounds is based on the peak area, retention time, molecular...

Author(s):Ghaidaa J. Mohammed, Imad H. Hameed and Sabreen A. Kamal

Article in Press

Opaque-2 Gene and Beta Carotene Content in 20 Yellow-, Red and Purple-Kernelled Maize (Zea Mays L.) Accessions in Ghana.

Twenty yellow-, red- and purple-kernel maize accessions were collected from three geographic regions in Ghana for the study. The objective was to determine the presence of opaque -2 gene and -carotene content of the grains as a means of assessing nutritional quality. Variability in -carotene content was determined by HPLC while presence of opaque 2-gene was determined by a light box. Significant variations were found in...

Author(s):GILBERT ANSAH and KOJO AHIAKPA

Article in Press

Microbial machinery based synthesis of nanoparticulate hydroxyapatite using Serratia marcescens and its characterization for human bone implant

Hydroxyapatite (HAp), chemically and compositionally similar to the material presents in bone and has a unique biocompatibility which makes it an attractive material for bone implant. We aimed at studying the physical and chemical properties of HAp synthesized by Serratia sp. The total of 58 strains was isolated from 354 samples obtained from clinical and environmental sources like urine, sputum, soil, water and plant...

Author(s):Rathinasamy Subashkumar, MIDHUSHA JOHNY and Nandhagopal Gayathri

Article in Press

Fatty acids profiling of the seed oils of some varieties of peanut (A.hypogaea L.) towards the development by GC-MS analysis.

Twelve fatty acids were identified during maturity in the wild Tunisian and cultivar peanut kernels. These fatty acids include C16:0 (palmitic acid), C18:0 (stearic acid), C18:1 (oleic acid), C18:2 (linoleic acid), C19:0 (nonadecanoic acid), C20:1 (gadoleic acid), C20:0 (arachidic acid), C22:1 (erucic acid), C22:0 (behenic acid), C23:0 (tricosanoic acid), C24:0 (linoceric acid), and C26:0 (cerotic acid). Three fatty acids...

Author(s):Aicha. O. CHERIF, Mhamed BEN MESSAOUDA, Habib KALLEL, Claude PEPE

Article in Press

Isolation of a novel strain Bacillus sp.in producing neutral and heat stable beta-1,4 endoglucanase activity

Neutral a β-1-4 - endoglucanase was produced by aerobic bacteria a newly strain Bacillus sp .RL. Isolated from soil, Chong Qing province, China. In the present study the bacteria produce a significant amount of enzymes after 72 hrs of fermentation CMC medium were 0.1852 U/ml of endoglucanase, 0.5814 U/ml of FPase activity and 2.9940 mg/ml of extracellular protein. These strains showed heavy growth and liquefaction of 7.0,...

Author(s):Rahman Laibi Chelab, Dan Dan Wang and Xingyong Yang

Article in Press

In vitro propagation as a viable alternative to the continuous regeneration of three Eucalyptus species (Eucalyptus camaldulensis, E. gomphocephala and E. maidenii)

The objective of this work was to propagate three species of Eucalyptus: Eucalyptus camaldulensis, Eucalyptus gomphocephala and Eucalyptus maideni on a large scale using microcutting technique. For the test of microcutting, from in vitro germination, the best rate of multiplication of 4.75 were obtained for E. camaldulensis on a medium containing 1 mg/L 6-benzylaminopurine (BAP) and 1 mg/L -aminoisobutyric acid (AIB) in...

Author(s):Hamrouni Lamia, Hanana Mohsen, Derbali Fedia, Hwass Haifa , Khouja Mohamed Larbi and Bouzid Sadok

Article in Press

Cloning and expression of recombinant human insulin gene in Pichia pastoris

The main goal of this current study was the cloning and expression of the human insulin gene in Pichia pastoris expression system, using genetic engineering techniques and its treatment application. Total RNA was purified from fresh normal human pancreatic tissue. RNA of good quality was chosen to obtain a first single strand cDNA. Human preproinsulin gene was amplified from cDNA strand using two sets of specific primers...

Author(s):Rafid A. Abdulkareem, Samah A. Jassam and Majid J. Jabber

Article in Press

Physico-chemical properties of Brazilian biomass: Potential applications as renewable energy source

Biomass thermal behavior under different atmosphere: combustion, pyrolysis (N2) and typical oxy-fuel combustion (60 and 80% CO2) were investigated by thermal analysis (TG/DTG and DTA). Other techniques such as X-Ray, Fourier Transformed Infrared (FTIR), Wettability, Scanning Electron Microscopy (SEM) and Inductive Couple Plasma - Optical Emission Spectrometer (ICP-OES) were also used to characterize the samples. Five...

Author(s):Glauber CRUZ, Carlos E. M. BRAZ, Ivonete ÁVILA and Paula M. CRNKOVIC

Article in Press

Establishment of high frequency regeneration protocol for Lycopersicon esculentum from leaf explant using seaweed extracts

The ultimate aim of this study is to standardize the conditions for in vitro organogenesis of tomato using plant growth regulators, organic elicitors, polyamines and seaweed extracts. Initially excised leaf explant was cultured on to the medium containing different concentrations of auxins. The best responded auxin was tested in combination with efficient cytokinins (Kinetin [Kin], 6-Benzylaminopurine [BAP] and Thidiazuron...

Author(s):S. Vinoth, P. Gurusaravanan, S. Sivakumar, G. Siva, G. Premkumar and N. Jayabalan

Article in Press

Characterization of lactic acid bacteria isolated from dromedary raw milk in Syria

Lactic acid bacteria (LAB) are considered a micro flora of Syrian camel milk. A total of 235 isolates of LAB were obtained from 63 camel milk samples, which have been collected from producing areas of different regions of Syria during 2009 and 2012. The isolates were cocci and rods, gram-positive and catalase-negative. 132 isolates were identified as cocci including 76 isolates belonging to Enterococcus (45 were...

Author(s):Ayman Al-Mariri, Ahed Abou Younes and Laila AL-Hallab

Article in Press

In silico analysis of antimicrobial eupalitin isolated from Ageratina adenophora using docking method

The present study evaluated the efficacy of eupalitin compounds against the target protein, dihydrofolate reductase of bacterial and fungal pathogens using in silico approach. Structure based docking studies was performed using Schrodinger (Version: 1.8) tool to retrieve potential candidates with high affinities binding against eupalitin models with several targeted proteins repositories. The specific targets were selected...

Author(s):Suganya Mani, Kavitha Shivaji, Karthik Natesan, Ashaq Hussain Bhat, Mythili Gnanamangai Balasubramanian and Ponmurugan Ponnusamy

Article in Press

In vitro screening of traditional rice varieties of Assam, India for drought tolerance

Eight traditional rice varieties viz., bora, prosad bhog, kola joha, helash bora, salihoi bao, kola amona, ronga bora and sok-bonglong along with ‘sahbhagi dhan’ (drought tolerant) and ‘IR64’ (drought sensitive) were studied invitro under two different drought stress conditions. Different parameters studied were germination percentage, shoot length and root length. Drought stress was produced by ½ Murashige and Skoog (MS)...

Author(s):Shamsun Nahar and Bhaben Tanti

Article in Press

Potential application of nutrigenomics in livestock: Lesson from developed countries

Livestock performance is affected by genotype and environment. Nutrition is one of the greatest environmental determinants. Genes function according to the metabolic signals received by the nucleus from hormones and nutrients. Currently, nutrigenomics, which encompasses the three crucial omics stages (transcriptomics, proteomics and metabolomics), is emerging in the area of livestock to develop feeds that can be...

Author(s):Bruh Asmelash Gebremikael, Mahlet Dawit Teweldebrihan and Haftom Brhane Mesfin

Page 3 of 3, showing 19 records out of 119 total, starting on record 101, ending on 119

123next >