African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002

AJB Articles in press

Article in Press

In vitro propagation of the wild medicinal plant, caper (Capparis spinosa L.)

Micropropagation of caper (Capparis spinosa L.) was done using wild leaves from Libya. High percentage (95%) of callus induction was obtained from Murashige and Skoog (MS) medium supplemented with 1.2 mg l-1 2,4-D + maltose. The effect of various concentrations (0.0, 0.5. 1.0 and 2.0 mg l-1) of cytokines (BAP, 6-γ-γ-[Dimethylallylamino]-purine 2ip, Kin and Zea) supplemented with MS medium was evaluated for callus...

Author(s):Adel M. Elmaghrabi, Elmundr Abughnia and Salem Hamoud

Article in Press

Role of Toll-like receptor 4 in regulating nucleotide-binding oligomerization domain2 (NOD2)-mediated inflammatory responses through Aspergillus fumigatus conidia stimulation in RAW264.7 macrophages

Nucleotide-binding oligomerization domain2 (NOD2) of the NOD-like receptors (NLRs) and Toll-like receptor4 (TLR4) of the TLRs are major pattern-recognition receptors. They sense different microbial pathogens and play important roles in innate immunity. Current in vivo and in vitro studies have shown that TLR4 and NOD2 act in anti-Aspergillus fumigatus infection. Herein, the cross-interaction between membrane-bound TLR4 and...

Author(s):MA Guang-qiang, WU Su-fang, LUO Hong-dan, XIE Wei-hua, LONG Kai, SU Ming-sheng and XIE Xiao-mei

Article in Press

The oxidative stability of linolenic fatty acid in farmed rainbow trout (Oncorhynchus mykiss) under different storage conditions in Isfahan City of Iran

The aim of this study was to investigate the effect of different storage conditions in market (ice box, glassy refrigerator, normal refrigerator without light (natural and electric power) and freezer) on alpha linolenic acid (ALA), a nutritional value of rainbow trout (Oncorhynchus mykiss). To investigate the effect of storage duration on fatty acid quality, samples stored after 1, 3 and 5 days in refrigerator and those...

Author(s):Lakzadeh, L., Mirmohammadi, M., Amouheidari, M. and Bavarian, A.

Article in Press

pression, Purification and On-Column Refolding of Recombinant S-layer Protein from L. acidophilus ATCC 4356

One of the most significant challenges in biotechnology is technological utilization of self-assembled systems. Surface (S-) layer technology is advanced due to the intrinsic property of subunits to reassemble into two-dimensional arrays. Lactobacillus acidophilus ATCC 4356 composed of S-layer protein (SlpA) subunits which are organized into regular arrays. Herein, S-layer gene (slpA) of L. acidophilus was cloned in...

Author(s):Elaheh Sadat Hosseini , Rouha Kasra Kermanshahi , Saman Hosseinkhani and Mahboobeh Nazari

Article in Press

Scanning electron microscope studies of pollen of herbaceous species of Amritsar, North-Western Punjab, India

Palynological studies in combination with conventional morphology have a significant role in solving problems relating to plant systematics. Scanning electron microscope (SEM) is a useful tool for palynological studies with increased accuracy and precision. The pollen size, shape and details of sculpturing of the exine are of taxonomic value. The main objective of the present study was to provide a detailed morphological...

Author(s):Harpreet Kaur and Avinash Nagpal

Article in Press

Merzouga Sand Dunes Actinobacteria: antimicrobial potential and taxonomic diversity

Merzouga sand dunes were among the Moroccan extreme environments. Actinobacteria from this under investigated desert habitat were isolated and their antimicrobial activity was evaluated. One hundred sixty three Actinobacteria isolates were isolated from sand collected from several dunes and screened for their antibacterial and antifungal activities. To test the antimicrobial effect of isolates, four microorganisms known as...

Author(s):Lahcen Ouchari, Amal Boukeskasse, Brahim Bouizgarne and Yedir Ouhdouch

Article in Press

Protocol optimization of in vitro propagation of an endangered endemic Ethiopia medicinal plant, Echinops kebericho (Mesfin), using shoot tip

Echinops kebericho (Mesfin)isthe most important and critically endangered endemic Ethiopian medicinal plant. This plant is threatened due to over harvesting of its roots for medicinal purpose, and from seed viability and seed germination problem. The aim of this research was to build up an efficient in vitro propagation protocol for E. kebericho using shoot tip explants. Shoots excised from in vitro germinated seedlings...

Author(s):Muluken Enyew; Tileye Feyissa

Article in Press

Development of phage mediated quartz crystal microbalance (QCM) sensor for the detection of Escherichia coli, a food-borne pathogen

This study presents the development of phage mediated quartz crystal microbalance (QCM) sensor for the detection of Escherichia coli. Bacteriophage is very specific to host bacterial cell also known as parasite for bacteria. The Electrochemical Quartz Crystal Microbalance (EQCM) is a very sensitive device that measures a mass change per unit area by measuring the change in resonant frequency of a quartz crystal. A...

Author(s):Vipin Singh and Pranay Jain

Article in Press

Morphological diversity studies among sugarcane (Saccharum spp.) somaclones generated through callus culture

Callogenesis is one of the tools in sugarcane tissue culture for generating agronomically significant variation. Tissue culture derived variations are known as somaclonal variation. The study was carried out to investigate the effect of MS media supplemented with 1.0 mgL-1 of NAA (naphthalene acetic acid) and 2.0, 2.5, 3.0, 3.5 and 4.0 mgL-1 of 2,4-D (2,4-dichlorophenoxy acetic acid) on somaclonal variation in in vitro...

Author(s):Richard Wekesa, Justus M. Onguso, Bernard A. Nyende and Leonard S. Wamocho

Article in Press

Biodegradation of crude oil by Bacillus species isolated from different sources

The consequences of oil pollution to the environment and human health as a result of exploration activities, transportation and usage of petroleum compounds, has become a serious challenge to environmental scientists. This study evaluates the potential of using Bacillus subtilis in biodegradation of petroleum compounds. Ten Bacillus strains from non-petroleum contaminated sites were identified on the basis of microscopy...

Author(s):Mohammed Inuwa Ja’afaru, Chimezie Godwin Azuchukwuene and Aisha Sa’id Shittu

Article in Press

Analytical quantification of copper in frogs (Rana tigrina) found from various aquatic habitats

This study focused on the quantitative determination of Cu metal in various physical parts of frogs by using the Atomic Absorption Spectrometry technique). Cu was measured (2.72±0.77 µg/g) in kidney of Rana tigrina captured from industrial site and in R. tigrina captured from non-industrial site (28.10±27.02 µg/g). Similarly, 27.56±9.92 µg/g Cu was discovered in liver of R. tigrina captured from industrial site and...

Author(s):Muhammad Nasir, Tariq Mahmood Ansari, Ghulam Yasin, Aleem Ahmad Khan and Muhammad Shoaib

Article in Press

Psychrotrophic proteolytic bacteria and their characterization from landfill soil under temperate climatic conditions of Kashmir Himalaya

During cold season in Kashmir valley, the decomposition of municipal solid waste slows down due to slowing down of microbial activity as a result, the waste gets piled up and its management becomes a greater challenge. Psychrotrophic proteolytic bacteria play an important role in the degradation of protenaceous materials of the waste stream under low temperatures that produces nitrogen sources for rapid functioning of...

Author(s):Basharat Hamid, Arshid Jehangir and Zahoor Ahmad Baba

Article in Press

Holistic plant regeneration protocol from callus cultures of Salvia moorcroftiana, a medicinal herb

Salvia moorcroftiana is a medicinal herb of the family Lamiaceae. The objective of this study was to develop an in vitro protocol for the rapid propagation of this species. Organogenesis by means of callus culture was carried out on Murashige and Skoog medium by the transfer of callus from an induction medium supplemented with α-naphtalenacetic acid (NAA, 1.0 mg/L) to regeneration medium containing 6-benzyladenin (BA, 1.0...

Author(s):Tour Jan, Ali Hazrat ,Rashid Azam, Arshad Ali Mohammad Nisar, Nasrullah Khan and Muhammad Asif Nawaz

Article in Press

Genetic variability, heritability and genetic advance for green pod yield and yield related traits in chili (Capsicum annum L.) accessions in Ethiopia

A field experiment was conducted to study the genetic variability, heritability and genetic advance for 12 quantitative characters in chili (Capsicum annum L.) accessions in Ethiopia, during the off-season period of 2012/2013 under flood irrigation at the Adet Agricultural Research Centre field. The experiment was conducted using Randomized Complete Block Design with three replications and a total of 25 accessions and four...

Author(s):Tadesse Tilahun, Yohannes Petros and Yigzaw Dessalegn

Article in Press

Effects of temperature and time on oil extraction from some Nigerian indigenous fresh water microalgae species

This study determined the effects of temperature and time on oil extraction from indigenous freshwater microalgae: Dictyosphaerium, Chlorella, Desmodesmus and Cosmarium species, cultured in 15 2-L column photobioreactors (PBR) (three per species). Growth and specific growth rates for the mixotrophic>autotrophic cultivation of the species were determined. Oil was extracted from the dried microalgae species at temperatures...

Author(s):Felix Uzochukwu Asoiro, Wilfred Ifeanyi Okonkwo, Clement Onyeaghala Akubuo, Nkechinyere O. Nweze and Cosmas Ngozichukwu Anyanwu

Article in Press

Atomic absorption spectroscopic evaluation of some heavy metal contents in tomato (Lycopersicon esculentum Mill) fruit and Ribu River water used for irrigation at Fodugora Farm, Weliso Town, Ethiopia

The levels of some heavy metals in mature fruits of tomato (Lycopersicon esculentum Mill) grown at Fodugora Farm, Weliso Town (Ethiopia) with Ribu River effluent water, used for its irrigation, have been determined using flame atomic absorption spectroscopic (FAAS) technique. Whereas, the levels of Fe, Zn and Pb in tomato fruits were: 34.05, 4.05, and 1.32 mg/L respectively, their concentrations in the river effluent water...

Author(s):Gera Techane, O. P. Yadav and LokeshYadav

Article in Press

Genetic diversity assessment of yams (Dioscorea spp.) from Ethiopia using inter simple sequence repeat (ISSR) markers

The genetic diversity and relationships of 70 accessions of yam belonging to Dioscorea cayenensis/Dioscorea rotundata complex (55), Dioscorea bulbifera (13) and Dioscorea alata (2, as a reference) were assessed using six inter simple sequence repeat (ISSR) primers. DNA was extracted from a bulk of two plants per accession using a modified cetyl trimethyl ammonium bromide (CTAB) method. Six ISSR primers amplified 77...

Author(s):Kedra Mohammed Ousmael

Article in Press

Study of secondary metabolites produced by Aspergillus flavus and evaluation of the antibacterial and antifungal activity

The objective of this study was the analysis of the secondary metabolite products and evaluation of antibacterial activity of Aspergillus flavus. Bioactives are chemical compounds often referred to as secondary metabolites. Thirty one bioactive compounds were identified in the methanolic extract of A. flavus. The identification of bioactive chemical compounds is based on the peak area, retention time, molecular...

Author(s):Ghaidaa J. Mohammed, Imad H. Hameed and Sabreen A. Kamal

Article in Press

Opaque-2 Gene and Beta Carotene Content in 20 Yellow-, Red and Purple-Kernelled Maize (Zea Mays L.) Accessions in Ghana.

Twenty yellow-, red- and purple-kernel maize accessions were collected from three geographic regions in Ghana for the study. The objective was to determine the presence of opaque -2 gene and -carotene content of the grains as a means of assessing nutritional quality. Variability in -carotene content was determined by HPLC while presence of opaque 2-gene was determined by a light box. Significant variations were found in...

Author(s):GILBERT ANSAH and KOJO AHIAKPA

Article in Press

Microbial machinery based synthesis of nanoparticulate hydroxyapatite using Serratia marcescens and its characterization for human bone implant

Hydroxyapatite (HAp), chemically and compositionally similar to the material presents in bone and has a unique biocompatibility which makes it an attractive material for bone implant. We aimed at studying the physical and chemical properties of HAp synthesized by Serratia sp. The total of 58 strains was isolated from 354 samples obtained from clinical and environmental sources like urine, sputum, soil, water and plant...

Author(s):Rathinasamy Subashkumar, MIDHUSHA JOHNY and Nandhagopal Gayathri

Article in Press

Fatty acids profiling of the seed oils of some varieties of peanut (A.hypogaea L.) towards the development by GC-MS analysis.

Twelve fatty acids were identified during maturity in the wild Tunisian and cultivar peanut kernels. These fatty acids include C16:0 (palmitic acid), C18:0 (stearic acid), C18:1 (oleic acid), C18:2 (linoleic acid), C19:0 (nonadecanoic acid), C20:1 (gadoleic acid), C20:0 (arachidic acid), C22:1 (erucic acid), C22:0 (behenic acid), C23:0 (tricosanoic acid), C24:0 (linoceric acid), and C26:0 (cerotic acid). Three fatty acids...

Author(s):Aicha. O. CHERIF, Mhamed BEN MESSAOUDA, Habib KALLEL, Claude PEPE

Article in Press

Isolation of a novel strain Bacillus sp.in producing neutral and heat stable beta-1,4 endoglucanase activity

Neutral a β-1-4 - endoglucanase was produced by aerobic bacteria a newly strain Bacillus sp .RL. Isolated from soil, Chong Qing province, China. In the present study the bacteria produce a significant amount of enzymes after 72 hrs of fermentation CMC medium were 0.1852 U/ml of endoglucanase, 0.5814 U/ml of FPase activity and 2.9940 mg/ml of extracellular protein. These strains showed heavy growth and liquefaction of 7.0,...

Author(s):Rahman Laibi Chelab, Dan Dan Wang and Xingyong Yang

Article in Press

In vitro propagation as a viable alternative to the continuous regeneration of three Eucalyptus species (Eucalyptus camaldulensis, E. gomphocephala and E. maidenii)

The objective of this work was to propagate three species of Eucalyptus: Eucalyptus camaldulensis, Eucalyptus gomphocephala and Eucalyptus maideni on a large scale using microcutting technique. For the test of microcutting, from in vitro germination, the best rate of multiplication of 4.75 were obtained for E. camaldulensis on a medium containing 1 mg/L 6-benzylaminopurine (BAP) and 1 mg/L -aminoisobutyric acid (AIB) in...

Author(s):Hamrouni Lamia, Hanana Mohsen, Derbali Fedia, Hwass Haifa , Khouja Mohamed Larbi and Bouzid Sadok

Article in Press

Cloning and expression of recombinant human insulin gene in Pichia pastoris

The main goal of this current study was the cloning and expression of the human insulin gene in Pichia pastoris expression system, using genetic engineering techniques and its treatment application. Total RNA was purified from fresh normal human pancreatic tissue. RNA of good quality was chosen to obtain a first single strand cDNA. Human preproinsulin gene was amplified from cDNA strand using two sets of specific primers...

Author(s):Rafid A. Abdulkareem, Samah A. Jassam and Majid J. Jabber

Article in Press

Physico-chemical properties of Brazilian biomass: Potential applications as renewable energy source

Biomass thermal behavior under different atmosphere: combustion, pyrolysis (N2) and typical oxy-fuel combustion (60 and 80% CO2) were investigated by thermal analysis (TG/DTG and DTA). Other techniques such as X-Ray, Fourier Transformed Infrared (FTIR), Wettability, Scanning Electron Microscopy (SEM) and Inductive Couple Plasma - Optical Emission Spectrometer (ICP-OES) were also used to characterize the samples. Five...

Author(s):Glauber CRUZ, Carlos E. M. BRAZ, Ivonete ÁVILA and Paula M. CRNKOVIC

Article in Press

Establishment of high frequency regeneration protocol for Lycopersicon esculentum from leaf explant using seaweed extracts

The ultimate aim of this study is to standardize the conditions for in vitro organogenesis of tomato using plant growth regulators, organic elicitors, polyamines and seaweed extracts. Initially excised leaf explant was cultured on to the medium containing different concentrations of auxins. The best responded auxin was tested in combination with efficient cytokinins (Kinetin [Kin], 6-Benzylaminopurine [BAP] and Thidiazuron...

Author(s):S. Vinoth, P. Gurusaravanan, S. Sivakumar, G. Siva, G. Premkumar and N. Jayabalan

Article in Press

Characterization of lactic acid bacteria isolated from dromedary raw milk in Syria

Lactic acid bacteria (LAB) are considered a micro flora of Syrian camel milk. A total of 235 isolates of LAB were obtained from 63 camel milk samples, which have been collected from producing areas of different regions of Syria during 2009 and 2012. The isolates were cocci and rods, gram-positive and catalase-negative. 132 isolates were identified as cocci including 76 isolates belonging to Enterococcus (45 were...

Author(s):Ayman Al-Mariri, Ahed Abou Younes and Laila AL-Hallab

Article in Press

In silico analysis of antimicrobial eupalitin isolated from Ageratina adenophora using docking method

The present study evaluated the efficacy of eupalitin compounds against the target protein, dihydrofolate reductase of bacterial and fungal pathogens using in silico approach. Structure based docking studies was performed using Schrodinger (Version: 1.8) tool to retrieve potential candidates with high affinities binding against eupalitin models with several targeted proteins repositories. The specific targets were selected...

Author(s):Suganya Mani, Kavitha Shivaji, Karthik Natesan, Ashaq Hussain Bhat, Mythili Gnanamangai Balasubramanian and Ponmurugan Ponnusamy

Article in Press

In vitro screening of traditional rice varieties of Assam, India for drought tolerance

Eight traditional rice varieties viz., bora, prosad bhog, kola joha, helash bora, salihoi bao, kola amona, ronga bora and sok-bonglong along with ‘sahbhagi dhan’ (drought tolerant) and ‘IR64’ (drought sensitive) were studied invitro under two different drought stress conditions. Different parameters studied were germination percentage, shoot length and root length. Drought stress was produced by ½ Murashige and Skoog (MS)...

Author(s):Shamsun Nahar and Bhaben Tanti

Article in Press

Potential application of nutrigenomics in livestock: Lesson from developed countries

Livestock performance is affected by genotype and environment. Nutrition is one of the greatest environmental determinants. Genes function according to the metabolic signals received by the nucleus from hormones and nutrients. Currently, nutrigenomics, which encompasses the three crucial omics stages (transcriptomics, proteomics and metabolomics), is emerging in the area of livestock to develop feeds that can be...

Author(s):Bruh Asmelash Gebremikael, Mahlet Dawit Teweldebrihan and Haftom Brhane Mesfin

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