African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Factors influencing the biosynthesis of 1-deoxynojirimycin in Morus alba L.

  De-Shuai Lou1, Feng-Ming Zou1, Hui Yan1 and Zhong-Zheng Gui1,2*    
  1School of Biology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China. 2Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
Email: [email protected]

  •  Accepted: 21 June 2011
  •  Published: 04 July 2011

Abstract

 

1-deoxynojirimycin (DNJ), a piperidine alkaloid enriched in mulberry, exhibits strong competitive inhibition against the α-glucosidase. To maximally use mulberry and to understand the mechanism of DNJ formation, we studied factors influencing DNJ biosynthesis in Morus alba. The results showed that the aqueous HCl immersion was a relative high efficiency method. We first revealed the relationship between DNJ content and factors including geographic locations, soil properties, meteorological factors and harvest time. They are: mulberry shoots taken from the top part of the branches had the highest content of DNJ, followed by roots, young leaves and mature leaves; mulberry growing in areas with higher latitude had higher content of DNJ; alkaline and low total nitrogen content in soil was advantageous to the biosynthesis of DNJ; the active peak periods of DNJ biosynthesis in a day appeared at 06:00 to 10:00 a.m. and 18:00 p.m.

 

Key words: Morus alba L., 1-deoxynojirimycin, biosynthesis, factors.