The study was carried out to assess whether nutrients could potentially affect chestnut trees caused by chestnut blight disease. These strains (JS-6, TC-4 and LT-1) were isolated and identified as Cryphonectriaparasitica by morphologic and molecular method. Cultures in the medium replaced carbon by one of six carbon sources, or nitrogen replaced by one of six nitrogen sources, or element added by one of four different minerals. The results showed the strains used a monosaccharide more efficiently than a disaccharide. The mycelial growth of strains cultured in the medium containing soluble amylum was not significantly different from the one in medium lacking a carbon source. Mycelial growth was more rapid when cultured in the medium containing Ca(NO3)2 rather than the other nitrogen sources investigated. However, there was no significant difference in mycelial growth in the medium where CO(NH2)2 was nitrogen and the medium that lacked nitrogen. The medium with 600 mg/L of MnCl2•4H2O inhibited the mycelial growth of strain LT-1, whereas 600 mg/L of Na2B4O7•10H2O increased mycelial growth. Strains JS-6 and TC-4 showed increased mycelial growth when CaCl2•4H2O was present in the medium. The results show the medium containing glucose, or CaCl2•4H2O, or Ca(NO3)2 could be used to increase the rate of mycelial growth in the laboratory and suggest avoiding soils with high calcium content for chestnut production and avoiding use of calcium fertilizers could potentially reduce the severity of the disease in chestnut trees.
Key words: Chestnut blight, nutrient components, growth of mycelia.
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