design (CRD) with four replications. Sodium hypochlorite 25% for 10 min with mercuric chloride 0.1% for 30 s and 35 had the best results for disinfection of initial leaves. Six callus induction treatments in Murashig and Skoog (MS) based medium were studied in a factorial experiment in a CRD with 4 replications. MS based medium supplemented with 2,4-D in 3 mg l-1 along with BAP in 0.3 mg l-1 was the best treatment for callus induction. Five regeneration treatments in modified MS were studied in a CRD with four replications. Modified MS supplemented with 1 mg l-1 BAP and NAA in 0.2 mg l-1 and also modified MS supplemented with Kinetin in 1 mg l-1 and NAA in 0.2 mg l-1 had the most effects on regeneration of calli. Five root treatments in half-strength MS medium were studied in a CRD with four replications. Hormone-free half-strength MS medium with IBA in 0.01 mg l-1 was efficient significantly for root formation of variety NCO310. Depicted dendrogram after analysis of electerophoresis data, classified regenerants in different groups indicating that, somaclonal variation could be an efficient way to create genetic variation particularly in plants propagated vegetatively.
Key words: Callus, regeneration, root, shoot, isozymes.
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