African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6730

Full Length Research Paper

The screening wheat (Triticum aestivum L.) plants for moderate phytate content using colorimetric values of semi-grain seeds

Xinglin Li1,2*, Tianjian Xiao1,2, Dandan Wang1,2, Jie Gao1, Liming Zhang1,2, Dong Liu1, Limei Li1, Fuping Lu1,2 and Wen-Yuan Gao3
1Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin, 300457, People’s Republic of China. 2College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China. 3College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin, 300072, People’s Republic of China.  
Email: [email protected]

  •  Accepted: 31 August 2010
  •  Published: 04 October 2010


To select wheat cultivars with moderate phytate content, in this experiment the authors found out a new method quickly screening individual plant in huge offspring. Twenty-three wheat cultivars were twice planted at two places. For one part of the seeds harvested, they randomly cut them into two semi-grain seeds one by one, the semi-grain seeds without embryos were assayed by colorimetric values in some micro-test plates, and the semi-grain seeds with embryos were reserved temporarily; whereas for another part seeds, they determined their phytate content and inorganic phosphorus content, respectively. The results indicated that, there were significantly negative correlations (p = 0.01) not only between the phytate content and the inorganic phosphorus content but also between the phytate content and the colorimetric value, whereas there was a significantly positive correlation (p = 0.01) between the inorganic phosphorus content and the colorimetric value. In conclusion, using colorimetric value of one semi-grain seed without embryo, wheat breeders might appraise phytate content of another semi-grain seed with embryo; if the colorimetric value of the semi-grain seeds without embryo is fit for moderate phytate content, the corresponding semi-grain seed with embryo would be reserved for next generation planting.


Key words: Colorimetric method, individual selection, inorganic phosphorus, phytate, semi-grain seed, Triticum aestivum L.