African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Acaricide resistance of Rhipicephalus microplus ticks in Benin

Safiou B. Adehan
  • Safiou B. Adehan
  • National Institute of Agronomic Research/Agronomic Research Centre of Agonkanmey 2900 Cotonou/Benin Ministry of Agriculture, Breeding and Fishing, Benin
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Abel Biguezoton
  • Abel Biguezoton
  • International Center of Research-Development on Breeding in Subhumid zone 01Box454 Bobo-Dioulasso01, Burkina-Faso
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Hassane Adakal
  • Hassane Adakal
  • International Center of Research-Development on Breeding in Subhumid zone 01Box454 Bobo-Dioulasso01, Burkina-Faso
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Marc N. Assogba
  • Marc N. Assogba
  • Polytecthnic School of Abomey-Calavi Research Unit in Biotechnology in Production and Animal Health O1 Box 2009 Cotonou, BÉNIN/Wecatic Project, Benin
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Sébastien Zoungrana
  • Sébastien Zoungrana
  • International Center of Research-Development on Breeding in Subhumid zone 01Box454 Bobo-Dioulasso01, Burkina-Faso
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A. Michel Gbaguidi
  • A. Michel Gbaguidi
  • National Institute of Agronomic Research/Agronomic Research Centre of Agonkanmey 2900 Cotonou/Benin Ministry of Agriculture, Breeding and Fishing, Benin
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Aretas Tonouhewa
  • Aretas Tonouhewa
  • Polytecthnic School of Abomey-Calavi Research Unit in Biotechnology in Production and Animal Health O1 Box 2009 Cotonou, BÉNIN/Wecatic Project, Benin
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Souleymane Kandé
  • Souleymane Kandé
  • Rural Development Institute (IDR)/Polytechnic University of Bobo-Dioulasso, Burkina Faso
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Louis Achi
  • Louis Achi
  • National Laboratory of Support to the Agricultural Development (LANADA) 04 BP 612 Abidjan 04, Côte d’Ivoire
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Hamade Kagone
  • Hamade Kagone
  • Manager of Livestock, Fisheries and Aquaculture Programme-CORAF/WECARD, Dakar, Senegal
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Razaki Adehan
  • Razaki Adehan
  • Polytecthnic School of Abomey-Calavi Research Unit in Biotechnology in Production and Animal Health O1 Box 2009 Cotonou, BÉNIN/Wecatic Project, Benin
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Guy A. Mensah
  • Guy A. Mensah
  • National Institute of Agronomic Research/Agronomic Research Centre of Agonkanmey 2900 Cotonou/Benin Ministry of Agriculture, Breeding and Fishing, Benin
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Reginald De Deken
  • Reginald De Deken
  • Vector Biology Unit, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium
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Maxime Madder
  • Maxime Madder
  • Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa
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Souaïbou Farougou
  • Souaïbou Farougou
  • Polytecthnic School of Abomey-Calavi Research Unit in Biotechnology in Production and Animal Health O1 Box 2009 Cotonou, BÉNIN/Wecatic Project, Benin
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  •  Received: 05 November 2015
  •  Accepted: 17 March 2016
  •  Published: 30 April 2016

Abstract

After the introduction of the invasive cattle tick Rhipicephalus microplus in West Africa in the last decade, farmers encounter  ticks resistance to the use of acaricides in different  region in Benin. In order to evaluate the level of resistance, an in vitro study was performed on five samples of R. (Boophilus) microplus collected from five farms in four of the eight agro-ecological zone of Benin. The districts concerned with the study in the agro-ecological zone were Houeyogbe (Kpinnou), Zangnanado (Samiondji), Tchaourou (Okpara), Gogounou (Fana) and Bassila (Manigri). A toxicological test, the Larval Packet Test (LPT) was performed in the laboratory of Biotechnology Research Unit of the Animal Production and Health (URBPSA) at the Polytechnic School of University of Abomey-Calavi in Benin with the susceptible, Rhipicephalus geigyi strain from Hounde in Burkina Faso. Three (3) acaricides commonly used by farmers in Benin to control ticks were evaluated: alpha-cypermethrin, deltamethrin and amitraz. The results showed that the resistance ratio at 50% (RR50 95% CI) for the whole experiment varies from 1.96 to 338.5. Based on RR50 and RR90 values, only the population of Samiondji’s state farm was susceptible to the alpha-cypermethrin with a resistance ratio RR50 = 1.64 (95% CI: 0.2 to 12.6 ), all the other resistance tests conducted on moderate or high resistance Bassila and Kpinnou appear to host the most resistant samples. Moreover, a certain high variability of dose response relationship has been noticed with amitraz on the base of the higher slope of the related curves.

 

Key words: Resistance ratio, distribution, Rhipicephalus microplus, acaricide, larval packet test (LPT), Benin