African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Isolation and molecular characterization of DgZFP2: A gene encoding a Cys2/His2-type zinc finger protein in chrysanthemum

Qing-Lin Liu1*, Fang-Li Dong2, Fang Xiao1, Jiao Wu1 and Zhu-Jing Li1      
1Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan 611130, P.R. China. 2Department of Biology, Hebei Jizhou High School, 86 Zhenshang Street, Jizhou, Hebei, 053200, P.R. China.  
Email: [email protected]

  •  Accepted: 22 June 2012
  •  Published: 21 August 2012

Abstract

The plant Cys2/His2-type zinc finger protein genes play critical roles in response to abiotic stresses. However, the roles of the chrysanthemum Cys2/His2-type zinc finger protein genes in plant stress responses are still not well known. In the present study, a full-length cDNA designated DgZFP2, containing two conserved Cys2/His2-type zinc finger motifs with a plant-specific QALGGH motif in each zinc finger domain, a L-box (EXEXXAXCLXXL), and an EAR-box (DLNL) at C-terminus, was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE). It encodes a protein of 178 amino acids residues with a calculated molecular mass of 20.05 kDa and theoretical isoelectric point of 8.24. The transcript of DgZFP2 was enriched in flowers and leaves than in roots and stems of the adult chrysanthemum plants. The gene expression was strongly induced by NaCl, drought, cold, and abscisic acid (ABA) treatment in the seedlings. We argued thatDgZFP2 is a new member of the Cys2/His2-type zinc finger protein genes, and it may be involved in the abiotic-stress response in plants.

 

Key words: Chrysanthemum, DgZFP2, gene expression, Cys2/His2-type zinc finger protein.