African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Assessment the genetic diversity of common bean Phaseolus vulgaris collection by microsatellite SSR markers

Jawaher M. Shabib, Afaf I. Shehata*, Amal A. Al–Hazzani and Muna Al-rumaih
Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh  11451, Saudi Arabia.
Email: [email protected]

  •  Accepted: 11 September 2013
  •  Published: 17 October 2013

Abstract

Common bean plants (Phaseolus vulgaris L.) are the most important edible beans for their high nutritional value as an important source of dietary protein. However, in spite of the importance of this crop species, its genetics has not been fully characterized. Advances in molecular biology techniques have provided the basis for uncovering virtually unlimited numbers of DNA markers. Polymerase Chain Reaction (PCR) -based markers are more convenient and accurate determination of the genetic relationships. The utility of PCR-DNA based marker is generally determined by the technology that is used to reveal DNA-based polymorphism between and within different organisms. Thus, the present investigation aimed to assess the genetic variability among the different varieties of common bean based on the genetic distances that obtained by simple sequence repeat (SSR) or microsatellite techniques. SSR-DNA markers will identify closely related genotypes of different varieties of common bean. In the present study, a total of eleven bean varieties, nine of P. vulgaris L., one of Phaseolus coccineus, and one of Phaseolus lunatus namely: Bean CORA, Judia cilena, Diamant, palati, Distinction, Contender, Fagiolo Nano, Ambra, Bronco, Merit, Lima bean were selected SSR markers that were relatively more efficient in detecting similarities among Pharsalus genotypes studied with similarities up to 95%. SSR profiling provides useful information on the level of polymorphism and genetic diversity in common bean.

 

Key words: Phaseolus vulgaris L., common bean, polymerase chain reaction, dietary protein.