African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Chemical composition and antibacterial activity of the essential oils of some medicinal plants

Mohammadi Abolfazl1, Malek Frhad2, Dehpour Ahmadreza3 and Nazari Hossein1*
1Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Science, Semnan, Iran. 2Department of Internal Medicine, Faculty of Medicine, Semnan University of Medical Science, Semnan, Iran. 3Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran.
Email: [email protected]

  •  Accepted: 19 June 2013
  •  Published: 27 June 2013

Abstract

Infectious diseases are the second leading cause of death worldwide. Treatment of infections continues to be a problem in modern time because of the side effects of some drugs and resistance to antimicrobial agents. Herbal medicines have received much attention as a source of safe and effective antibacterial drugs. In the present study, essential oils of some medicinal plants: Stachys pubescensThymus kotschyanus, Thymus daenensis and Bupleurum falcatum were investigated for antibacterial activity against Staphylococcus aureus, Listeria monocytogenes, Streptococcus pneumoniae, Pseudomonas aeruginosa, Klebsiella pneumoniae,Escherichia coli, and Salmonella typhi. This study determines the MIC and MBC of the essential oils and comparison with reference antibiotics "Ciprofloxacin and Gentamicin". The chemical composition of the essential oils was analyzed by Gas chromatography (GC) and Gas chromatography-mass spectrometry (GC-MS). The essential oils isolated from the leaves of S. pubescensT. kotschyanus and B. falcatum showed a high level antibacterial activity. T. daenensis essential oil showed less activity comparing to other oils. The result showed the presence of 24, 23, 20 and 15 components in the essential oils of T. kotschyanusS. pubescens,T. daenensis, and B. falcatum respectively. Resistance to first-line drugs in most of the pathogens causing these diseases ranges from zero to almost 100%. Thus, these essential oils exhibited interesting bacteriostatic and bactericidal activity.

 

Key words: Antibacterial, Stachys pubescensThymus kotschyanus, Thymus daenensis. Bupleurum falcatum, GC-MS.