African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Short Communication

Peroxidase and polyphenol oxidase activity on the yield of grafted and ungrafted cucumber plants

Douglas Seijum Kohatsu
  • Douglas Seijum Kohatsu
  • Professores Doutores, UEM/CCA/ DCA Agronomia Umuarama – PR, Brazil.
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Valdir Zucareli
  • Valdir Zucareli
  • Professores Doutores, UEM/CCA/ DCA Agronomia Umuarama – PR, Brazil.
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Wilian Polaco Brambilla
  • Wilian Polaco Brambilla
  • Pós graduando em Agronomia, Unesp - Botucatu-SP, Brazil.
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Elizabeth Orika Ono
  • Elizabeth Orika Ono
  • Professores Doutores, Departamento de Botânica, Instituto de Biociências, Unesp - Botucatu-SP, Brazil.
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Tiago Roque Benetoli da Silva
  • Tiago Roque Benetoli da Silva
  • Professores Doutores, UEM/CCA/ DCA Agronomia Umuarama – PR, Brazil.
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João Domingos Rodrigues
  • João Domingos Rodrigues
  • Professores Doutores, Departamento de Botânica, Instituto de Biociências, Unesp - Botucatu-SP, Brazil.
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  •  Accepted: 19 December 2012
  •  Published: 31 January 2013

Abstract

 

The first experiment was carried out under green house and involved nine treatments: ‘Tsuyoi’ cucumber, ‘Shelper’ squash and ‘Green-stripped cushaw squash’ ungrafted plants and ‘Tsuyoi’ cucumber plants grafted onto ‘Shelper’ squash and ‘Green-stripped cushaw squash (lower, mid and upper region of the recommended and non-recommended rootstock, respectively). After grafting, plant tissue samples were collected 1, 4, 7, 10 and 13 days after grafting for analysis of peroxidase (EC 1.11.1.7) and polyphenol oxidase (EC 1.10.3.1) activity. In the second experiment, yield and number of marketable fruits were evaluated. The differences in peroxidase activity at the rootstock region and in polyphenol activity at the region between the scion and the rootstock seem to be determining factors for a successful grafting process, increasing the yield and the number of marketable fruits.

 

Key wordsCucumis sativus, antioxidant enzymes, yield, Japanese cucumber.