African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6709

Full Length Research Paper

Influence of seed co-inoculation with Bradyrhizobium species and Azospirillum brasilense on soybean development in Southern and Southeastern Brazil

Alessandro Lucca Braccini
  • Alessandro Lucca Braccini
  • Department of Agronomy, Maringa State University (UEM), CEP 87020-900, Maringa, PR, Brazil.
  • Google Scholar
Marcos Vinícius Ribas Milléo
  • Marcos Vinícius Ribas Milléo
  • Department of Agronomy, Ponta Grossa State University (UEPG), CEP 84030-900, Ponta Grossa, PR, Brazil.
  • Google Scholar
Bernardo Alleoni
  • Bernardo Alleoni
  • Agroplanbras Serviços Agrícolas Ltda., Road PR 438, Km 46 – Distrito de Guaragi, Ponta Grossa, PR, Brazil.
  • Google Scholar
Artur Berbel Lirio Rondina
  • Artur Berbel Lirio Rondina
  • Biology Department, Centro Universitário de Ourinhos (Unifio), Road BR-153, km 338 S/N, Ourinhos – SP, Brazil.
  • Google Scholar
Fabiane Paulitsch
  • Fabiane Paulitsch
  • Department of Structural and Molecular Biology and Genetics, Ponta Grossa State University (UEPG), CEP 84030-900, Ponta Grossa, PR, Brazil.
  • Google Scholar
Luciane Muller Martins
  • Luciane Muller Martins
  • Agrocete Industria de Fertilizantes Ltda, CEP 84043-465, Ponta Grossa, PR, Brazil.
  • Google Scholar
Fernanda Gravina
  • Fernanda Gravina
  • Agrocete Industria de Fertilizantes Ltda, CEP 84043-465, Ponta Grossa, PR, Brazil.
  • Google Scholar
Tárik Hanai Yoshida
  • Tárik Hanai Yoshida
  • Agrocete Industria de Fertilizantes Ltda, CEP 84043-465, Ponta Grossa, PR, Brazil.
  • Google Scholar
Fernanda Gravina
  • Fernanda Gravina
  • Agrocete Industria de Fertilizantes Ltda, CEP 84043-465, Ponta Grossa, PR, Brazil.
  • Google Scholar
Vinícius Tavares Ávila
  • Vinícius Tavares Ávila
  • Agrocete Industria de Fertilizantes Ltda, CEP 84043-465, Ponta Grossa, PR, Brazil.
  • Google Scholar
Fernanda M. Rampazzo Schena de Figueiredo
  • Fernanda M. Rampazzo Schena de Figueiredo
  • Agrocete Industria de Fertilizantes Ltda, CEP 84043-465, Ponta Grossa, PR, Brazil.
  • Google Scholar
Raul Almeida
  • Raul Almeida
  • Agrocete Industria de Fertilizantes Ltda, CEP 84043-465, Ponta Grossa, PR, Brazil.
  • Google Scholar


  •  Received: 03 October 2022
  •  Accepted: 06 January 2023
  •  Published: 31 January 2023

Abstract

Bioinoculants are widely used in Brazil, based on efficient and low-cost. This study aimed to evaluate the agronomic efficiency of co-inoculation with Bradyrhizobium species and Azospirillum brasilense in soybean seeds. Field trials were established in four regions of Brazil using a randomized block design  with 8 treatments and 4 replications as follows: T1 - Control, T2 - 200 kg ha-1 N, T3 - Bradyrhizobium spp. – peat; T4 - Bradyrhizobium spp. - liquid, T5 – A. brasilense, T6 - Standard co-inoculation Bradyrhizobium spp. + A. brasilense, T7 - Co-inoculation ratios: 1:1 Bradyrhizobium ssp. GRAP NOD L®  + A. brasilense GRAP NOD AL®, T8 - Co-inoculation ratios: 1:1.5 Bradyrhizobium ssp. GRAP NOD L® + A. brasilense GRAP NOD AL®. The study results demonstrated the influence of co-inoculation 1:1.5, providing increments in shoots and root dry mass, nodules number and dry mass, pods number per plant, and yield when compared with the control and conventional N fertilization. The co-inoculation in the ratios 1:1 and 1:1.5, positively influenced the soybean development. These results confirmed that potential of the co-inoculation in incrementing soybean yield, which justify the recommendation of these treatments to reduce mineral N fertilization, improving good agronomic practices.

Key words: Rhizobia, Glycine max, BNF, PGPB, inoculation, plant nutrition, crop yield, plant-bacteria symbiosis, nitrogen fertilizer.