African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Analysis of protein by colorimetric and computer color based intensity measurement method with anatomical sections of gram (Cicer arietinum) stem at three stages of growth

Shah A. M.1*, Memon M. S.1, Arain B. A.2, Ansari A. W.3 and Memon A. N.1    
1Institute of Biochemistry, University of Sindh, Jamshoro, Sindh, Pakistan. 2Institute of Botany, University of Sindh, Jamshoro, Sindh, Pakistan. 3Department of Computer Science, ISRA University, Hyderabad, Sindh, Pakistan.    
Email: [email protected] , [email protected]

  •  Published: 26 May 2012

Abstract

This research work is based on the establishment of new and easy techniques for the analysis of total proteins from anatomical sections. The proteins were measured from three different growth stages of stems of gram (Cicer arietinum). Firstly, protein was measured by colorimetric method and then analyzed from the cross sections of the same part of plant, sections were then stained with two different dyes and after that color intensity of stained sections was measured through computer software. Results of Lowry’s and Bradford methods showed that the third stage of both plants have a maximum amount of proteins. The staining period of dye was also established, the staining of sections (stems of gram) with all dyes gave maximum amount of protein at 2 h staining when compared to 1, 3 and 24 h of staining periods. The results of the computer analysis showed more concentration of protein content when compared to colorimetric methods in the visible regions. By this method, we can see the migration, destination and distribution of different compounds from the root to the top of the plant in the near future.

 

Key words: Colorimetric methods, stained anatomical sections, computer software.