African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Short Communication

Characterization of novel microsatellite loci for clam Moerella iridescens (Benson)

Jian-xin Wang1, Tian-jun Xu1, Li Wei2, Fan-xing Meng1, Ge Shi1 and Ri-xin Wang1*  
1Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316000, Peoples Republic of China. 2College of Life Science, East China Normal University, Shanghai 200062, China.
Email: [email protected]

  •  Accepted: 27 July 2010
  •  Published: 18 August 2011

Abstract

From the (CA)15 enriched genomic libraries of Moerella iridescens, eleven novel polymorphic microsatellite loci were isolated and characterized in M. iridescens (Benson), an important species of marine economic shellfish. The mean number of observed alleles per locus was 18 (range 5 – 34). The observed and expected heterozygosity values ranged from 0.043 to 0.979 and from 0.162 to 0.966 respectively. Three loci showed significant departure from Hardy-Weinberg equilibrium and non-significant linkage disequilibrium was found among all loci. These highly informative microsatellite markers should be useful for population genetic analyses of M. iridescens.

 

Key words: Microsatellite, Moerella iridescens, genetic diversity, polymorphism.